Clinical, Functional and Structural Studies of Humans and Merino Sheep Corneas from Two Different Regions of Argentina

Autores
Suarez, Maria Fernanda; Crim, Nicolás; Monti, Rodolfo; Esposito, Evangelina; Urrets Zavalía, Julio Alberto; Serra, Horacio Marcelo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: The purpose of our work was to study and compare the cornea and tear film of people and sheep inhabiting in a region of Argentinean Patagonia (PATG), and in a geographically and climatically different region in the Argentinean Pampa (CAPT).Methods: Using cross sectional study, corneal examinations were performed in people and Merino sheep inhabiting PATG and CAPT regions, respectively. All people completed a questionnaire related to work activity, diet, and the use of hats or sunglasses during their life. Eyes from all participants were examined with a portable hand- held slit-lamp biomicroscope (BM) to evaluate corneal appearance, epithelium integrity, and transparency. Later on, in a subgroup of participants we studied eye blinking frequency (EBF), ocular surface staining (FS), breakup time (BUT), Schirmer Tear Test (STT), corneal structure using confocal laser scanning microscopy (CLSM) and light microscopy (LM), and concentrations of serum ascorbate (sAA).Results: BM studies revealed numerous cases of climatic droplet keratopathy (CDK) only in people inhabiting the PATG region. CLSM studies confirmed typical punctate deposits at the Bowman ́s layer in CDK patients living in that region. CLSM images from sheep did not show any abnormalities at the Bowman ́s layer but exhibited small hyper reflective dots at the epithelium only in animals pasturing in the PATG region. FS and EBF mean values were significant higher in sheep grazing in the PATG region (p <0.05). No differences were found in surface eye tests when people from both regions were analyzed. Low sAA levels were found only in CDK people living in PATG region.Conclusions: CDK is a multi-factorial disease not only associated to harsh climate. We provide data that low sAA levels can play a role in its genesis in Patagonia patients?... Sheep pasturing in this region with a high prevalence of CDK have high concentration of sAA and did not present any sub epithelial corneal abnormalities.
Fil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Crim, Nicolás. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola; Argentina
Fil: Monti, Rodolfo. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola; Argentina
Fil: Esposito, Evangelina. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola; Argentina
Fil: Urrets Zavalía, Julio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola; Argentina
Fil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Materia
CLIMATIC DROPLET KERATOPATHY
CORNEAL DEGENERATION
ARGENTINIAN PATAGONIA
ARGENTINIAN PAMPA
HUMAN
SHEEP
OCULAR SURGACE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/46689

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network_name_str CONICET Digital (CONICET)
spelling Clinical, Functional and Structural Studies of Humans and Merino Sheep Corneas from Two Different Regions of ArgentinaSuarez, Maria FernandaCrim, NicolásMonti, RodolfoEsposito, EvangelinaUrrets Zavalía, Julio AlbertoSerra, Horacio MarceloCLIMATIC DROPLET KERATOPATHYCORNEAL DEGENERATIONARGENTINIAN PATAGONIAARGENTINIAN PAMPAHUMANSHEEPOCULAR SURGACEhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Objective: The purpose of our work was to study and compare the cornea and tear film of people and sheep inhabiting in a region of Argentinean Patagonia (PATG), and in a geographically and climatically different region in the Argentinean Pampa (CAPT).Methods: Using cross sectional study, corneal examinations were performed in people and Merino sheep inhabiting PATG and CAPT regions, respectively. All people completed a questionnaire related to work activity, diet, and the use of hats or sunglasses during their life. Eyes from all participants were examined with a portable hand- held slit-lamp biomicroscope (BM) to evaluate corneal appearance, epithelium integrity, and transparency. Later on, in a subgroup of participants we studied eye blinking frequency (EBF), ocular surface staining (FS), breakup time (BUT), Schirmer Tear Test (STT), corneal structure using confocal laser scanning microscopy (CLSM) and light microscopy (LM), and concentrations of serum ascorbate (sAA).Results: BM studies revealed numerous cases of climatic droplet keratopathy (CDK) only in people inhabiting the PATG region. CLSM studies confirmed typical punctate deposits at the Bowman ́s layer in CDK patients living in that region. CLSM images from sheep did not show any abnormalities at the Bowman ́s layer but exhibited small hyper reflective dots at the epithelium only in animals pasturing in the PATG region. FS and EBF mean values were significant higher in sheep grazing in the PATG region (p <0.05). No differences were found in surface eye tests when people from both regions were analyzed. Low sAA levels were found only in CDK people living in PATG region.Conclusions: CDK is a multi-factorial disease not only associated to harsh climate. We provide data that low sAA levels can play a role in its genesis in Patagonia patients?... Sheep pasturing in this region with a high prevalence of CDK have high concentration of sAA and did not present any sub epithelial corneal abnormalities.Fil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Crim, Nicolás. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola; ArgentinaFil: Monti, Rodolfo. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola; ArgentinaFil: Esposito, Evangelina. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola; ArgentinaFil: Urrets Zavalía, Julio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola; ArgentinaFil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaWiley Blackwell Publishing, Inc2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/46689Suarez, Maria Fernanda; Crim, Nicolás; Monti, Rodolfo; Esposito, Evangelina; Urrets Zavalía, Julio Alberto; et al.; Clinical, Functional and Structural Studies of Humans and Merino Sheep Corneas from Two Different Regions of Argentina; Wiley Blackwell Publishing, Inc; Journal of Clinical & Experimental Ophthalmology; 06; 06; 11-2015; 1-82155-95701442-6404CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2155-9570.1000499info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/clinical-functional-and-structural-studies-of-humans-and-merino-sheepcorneas-from-two-different-regions-of-argentina-2155-9570-1000499.php?aid=66076info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:58Zoai:ri.conicet.gov.ar:11336/46689instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:58.894CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Clinical, Functional and Structural Studies of Humans and Merino Sheep Corneas from Two Different Regions of Argentina
title Clinical, Functional and Structural Studies of Humans and Merino Sheep Corneas from Two Different Regions of Argentina
spellingShingle Clinical, Functional and Structural Studies of Humans and Merino Sheep Corneas from Two Different Regions of Argentina
Suarez, Maria Fernanda
CLIMATIC DROPLET KERATOPATHY
CORNEAL DEGENERATION
ARGENTINIAN PATAGONIA
ARGENTINIAN PAMPA
HUMAN
SHEEP
OCULAR SURGACE
title_short Clinical, Functional and Structural Studies of Humans and Merino Sheep Corneas from Two Different Regions of Argentina
title_full Clinical, Functional and Structural Studies of Humans and Merino Sheep Corneas from Two Different Regions of Argentina
title_fullStr Clinical, Functional and Structural Studies of Humans and Merino Sheep Corneas from Two Different Regions of Argentina
title_full_unstemmed Clinical, Functional and Structural Studies of Humans and Merino Sheep Corneas from Two Different Regions of Argentina
title_sort Clinical, Functional and Structural Studies of Humans and Merino Sheep Corneas from Two Different Regions of Argentina
dc.creator.none.fl_str_mv Suarez, Maria Fernanda
Crim, Nicolás
Monti, Rodolfo
Esposito, Evangelina
Urrets Zavalía, Julio Alberto
Serra, Horacio Marcelo
author Suarez, Maria Fernanda
author_facet Suarez, Maria Fernanda
Crim, Nicolás
Monti, Rodolfo
Esposito, Evangelina
Urrets Zavalía, Julio Alberto
Serra, Horacio Marcelo
author_role author
author2 Crim, Nicolás
Monti, Rodolfo
Esposito, Evangelina
Urrets Zavalía, Julio Alberto
Serra, Horacio Marcelo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CLIMATIC DROPLET KERATOPATHY
CORNEAL DEGENERATION
ARGENTINIAN PATAGONIA
ARGENTINIAN PAMPA
HUMAN
SHEEP
OCULAR SURGACE
topic CLIMATIC DROPLET KERATOPATHY
CORNEAL DEGENERATION
ARGENTINIAN PATAGONIA
ARGENTINIAN PAMPA
HUMAN
SHEEP
OCULAR SURGACE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective: The purpose of our work was to study and compare the cornea and tear film of people and sheep inhabiting in a region of Argentinean Patagonia (PATG), and in a geographically and climatically different region in the Argentinean Pampa (CAPT).Methods: Using cross sectional study, corneal examinations were performed in people and Merino sheep inhabiting PATG and CAPT regions, respectively. All people completed a questionnaire related to work activity, diet, and the use of hats or sunglasses during their life. Eyes from all participants were examined with a portable hand- held slit-lamp biomicroscope (BM) to evaluate corneal appearance, epithelium integrity, and transparency. Later on, in a subgroup of participants we studied eye blinking frequency (EBF), ocular surface staining (FS), breakup time (BUT), Schirmer Tear Test (STT), corneal structure using confocal laser scanning microscopy (CLSM) and light microscopy (LM), and concentrations of serum ascorbate (sAA).Results: BM studies revealed numerous cases of climatic droplet keratopathy (CDK) only in people inhabiting the PATG region. CLSM studies confirmed typical punctate deposits at the Bowman ́s layer in CDK patients living in that region. CLSM images from sheep did not show any abnormalities at the Bowman ́s layer but exhibited small hyper reflective dots at the epithelium only in animals pasturing in the PATG region. FS and EBF mean values were significant higher in sheep grazing in the PATG region (p <0.05). No differences were found in surface eye tests when people from both regions were analyzed. Low sAA levels were found only in CDK people living in PATG region.Conclusions: CDK is a multi-factorial disease not only associated to harsh climate. We provide data that low sAA levels can play a role in its genesis in Patagonia patients?... Sheep pasturing in this region with a high prevalence of CDK have high concentration of sAA and did not present any sub epithelial corneal abnormalities.
Fil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Crim, Nicolás. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola; Argentina
Fil: Monti, Rodolfo. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola; Argentina
Fil: Esposito, Evangelina. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola; Argentina
Fil: Urrets Zavalía, Julio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola; Argentina
Fil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
description Objective: The purpose of our work was to study and compare the cornea and tear film of people and sheep inhabiting in a region of Argentinean Patagonia (PATG), and in a geographically and climatically different region in the Argentinean Pampa (CAPT).Methods: Using cross sectional study, corneal examinations were performed in people and Merino sheep inhabiting PATG and CAPT regions, respectively. All people completed a questionnaire related to work activity, diet, and the use of hats or sunglasses during their life. Eyes from all participants were examined with a portable hand- held slit-lamp biomicroscope (BM) to evaluate corneal appearance, epithelium integrity, and transparency. Later on, in a subgroup of participants we studied eye blinking frequency (EBF), ocular surface staining (FS), breakup time (BUT), Schirmer Tear Test (STT), corneal structure using confocal laser scanning microscopy (CLSM) and light microscopy (LM), and concentrations of serum ascorbate (sAA).Results: BM studies revealed numerous cases of climatic droplet keratopathy (CDK) only in people inhabiting the PATG region. CLSM studies confirmed typical punctate deposits at the Bowman ́s layer in CDK patients living in that region. CLSM images from sheep did not show any abnormalities at the Bowman ́s layer but exhibited small hyper reflective dots at the epithelium only in animals pasturing in the PATG region. FS and EBF mean values were significant higher in sheep grazing in the PATG region (p <0.05). No differences were found in surface eye tests when people from both regions were analyzed. Low sAA levels were found only in CDK people living in PATG region.Conclusions: CDK is a multi-factorial disease not only associated to harsh climate. We provide data that low sAA levels can play a role in its genesis in Patagonia patients?... Sheep pasturing in this region with a high prevalence of CDK have high concentration of sAA and did not present any sub epithelial corneal abnormalities.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/46689
Suarez, Maria Fernanda; Crim, Nicolás; Monti, Rodolfo; Esposito, Evangelina; Urrets Zavalía, Julio Alberto; et al.; Clinical, Functional and Structural Studies of Humans and Merino Sheep Corneas from Two Different Regions of Argentina; Wiley Blackwell Publishing, Inc; Journal of Clinical & Experimental Ophthalmology; 06; 06; 11-2015; 1-8
2155-9570
1442-6404
CONICET Digital
CONICET
url http://hdl.handle.net/11336/46689
identifier_str_mv Suarez, Maria Fernanda; Crim, Nicolás; Monti, Rodolfo; Esposito, Evangelina; Urrets Zavalía, Julio Alberto; et al.; Clinical, Functional and Structural Studies of Humans and Merino Sheep Corneas from Two Different Regions of Argentina; Wiley Blackwell Publishing, Inc; Journal of Clinical & Experimental Ophthalmology; 06; 06; 11-2015; 1-8
2155-9570
1442-6404
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4172/2155-9570.1000499
info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/open-access/clinical-functional-and-structural-studies-of-humans-and-merino-sheepcorneas-from-two-different-regions-of-argentina-2155-9570-1000499.php?aid=66076
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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