Brain development is impaired in c-fos -/- mice

Autores
Velazquez, Fabiola Noelia; Prucca, Cesar German; Etienne, Olivier; D'Astolfo, Diego S.; Silvestre, David C.; Boussin, Francois D.; Caputto, Beatriz Leonor
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
c-Fos is a proto-oncogene involved in diverse cellular functions. Its deregulation has been associated to abnormal development and oncogenic progression. c-fos-/- mice are viable but present a reduction in their body weight and brain size. We examined the importance of c-Fos during neocortex development at 13.5, 14.5 and 16.5 days of gestation. At E14.5, neocortex thickness, apoptosis, mitosis and expression of markers along the different stages of Neural Stem Progenitor Cells (NSPCs) differentiation in c-fos-/- and wild-type mice were analyzed. A ~15% reduction in the neocortex thickness of c-fos-/- embryos was observed which correlates with a decrease in the number of differentiated cells and an increase in apoptosis at the ventricular zone. No difference in mitosis rate was observed, although the mitotic angle was predominantly vertical in c-fos-/- embryos, suggesting a reduced trend of NSPCs to differentiate. At E13.5, changes in differentiation markers start to be apparent and are still clearly observed at E16.5. A tendency of more AP-1/DNA complexes present in nuclear extracts of cerebral cortex from c-fos-/- embryos with no differences in the lipid synthesis activity was found. These results suggest that c-Fos is involved in the normal development of NSPCs by means of its AP-1 activity.
Fil: Velazquez, Fabiola Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Prucca, Cesar German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Etienne, Olivier. Laboratoire de Radiopathologie, Cea, Irsm; Francia. Université Paris Diderot - Paris 7; Francia
Fil: D'Astolfo, Diego S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Silvestre, David C.. Laboratoire de Radiopathologie, Cea, Irsm; Francia. Université Paris Diderot - Paris 7; Francia
Fil: Boussin, Francois D.. Université Paris Diderot - Paris 7; Francia. Laboratoire de Radiopathologie, Cea, Irsm; Francia
Fil: Caputto, Beatriz Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Materia
APOPTOSIS
DIFFERENTIATION
NEOCORTEX
NEURAL STEM PROGENITOR CELLS (NSPCS)
NEUROGENESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50818
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/50818
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Brain development is impaired in c-fos -/- miceVelazquez, Fabiola NoeliaPrucca, Cesar GermanEtienne, OlivierD'Astolfo, Diego S.Silvestre, David C.Boussin, Francois D.Caputto, Beatriz LeonorAPOPTOSISDIFFERENTIATIONNEOCORTEXNEURAL STEM PROGENITOR CELLS (NSPCS)NEUROGENESIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1c-Fos is a proto-oncogene involved in diverse cellular functions. Its deregulation has been associated to abnormal development and oncogenic progression. c-fos-/- mice are viable but present a reduction in their body weight and brain size. We examined the importance of c-Fos during neocortex development at 13.5, 14.5 and 16.5 days of gestation. At E14.5, neocortex thickness, apoptosis, mitosis and expression of markers along the different stages of Neural Stem Progenitor Cells (NSPCs) differentiation in c-fos-/- and wild-type mice were analyzed. A ~15% reduction in the neocortex thickness of c-fos-/- embryos was observed which correlates with a decrease in the number of differentiated cells and an increase in apoptosis at the ventricular zone. No difference in mitosis rate was observed, although the mitotic angle was predominantly vertical in c-fos-/- embryos, suggesting a reduced trend of NSPCs to differentiate. At E13.5, changes in differentiation markers start to be apparent and are still clearly observed at E16.5. A tendency of more AP-1/DNA complexes present in nuclear extracts of cerebral cortex from c-fos-/- embryos with no differences in the lipid synthesis activity was found. These results suggest that c-Fos is involved in the normal development of NSPCs by means of its AP-1 activity.Fil: Velazquez, Fabiola Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Prucca, Cesar German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Etienne, Olivier. Laboratoire de Radiopathologie, Cea, Irsm; Francia. Université Paris Diderot - Paris 7; FranciaFil: D'Astolfo, Diego S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Silvestre, David C.. Laboratoire de Radiopathologie, Cea, Irsm; Francia. Université Paris Diderot - Paris 7; FranciaFil: Boussin, Francois D.. Université Paris Diderot - Paris 7; Francia. Laboratoire de Radiopathologie, Cea, Irsm; FranciaFil: Caputto, Beatriz Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaImpact Journals LLC2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50818Velazquez, Fabiola Noelia; Prucca, Cesar German; Etienne, Olivier; D'Astolfo, Diego S.; Silvestre, David C.; et al.; Brain development is impaired in c-fos -/- mice; Impact Journals LLC; Oncotarget; 6; 19; 7-2015; 16883-169011949-2553CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/26143639info:eu-repo/semantics/altIdentifier/doi/10.18632/oncotarget.4527info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:23Zoai:ri.conicet.gov.ar:11336/50818instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:23.678CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Brain development is impaired in c-fos -/- mice
title Brain development is impaired in c-fos -/- mice
spellingShingle Brain development is impaired in c-fos -/- mice
Velazquez, Fabiola Noelia
APOPTOSIS
DIFFERENTIATION
NEOCORTEX
NEURAL STEM PROGENITOR CELLS (NSPCS)
NEUROGENESIS
title_short Brain development is impaired in c-fos -/- mice
title_full Brain development is impaired in c-fos -/- mice
title_fullStr Brain development is impaired in c-fos -/- mice
title_full_unstemmed Brain development is impaired in c-fos -/- mice
title_sort Brain development is impaired in c-fos -/- mice
dc.creator.none.fl_str_mv Velazquez, Fabiola Noelia
Prucca, Cesar German
Etienne, Olivier
D'Astolfo, Diego S.
Silvestre, David C.
Boussin, Francois D.
Caputto, Beatriz Leonor
author Velazquez, Fabiola Noelia
author_facet Velazquez, Fabiola Noelia
Prucca, Cesar German
Etienne, Olivier
D'Astolfo, Diego S.
Silvestre, David C.
Boussin, Francois D.
Caputto, Beatriz Leonor
author_role author
author2 Prucca, Cesar German
Etienne, Olivier
D'Astolfo, Diego S.
Silvestre, David C.
Boussin, Francois D.
Caputto, Beatriz Leonor
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
DIFFERENTIATION
NEOCORTEX
NEURAL STEM PROGENITOR CELLS (NSPCS)
NEUROGENESIS
topic APOPTOSIS
DIFFERENTIATION
NEOCORTEX
NEURAL STEM PROGENITOR CELLS (NSPCS)
NEUROGENESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv c-Fos is a proto-oncogene involved in diverse cellular functions. Its deregulation has been associated to abnormal development and oncogenic progression. c-fos-/- mice are viable but present a reduction in their body weight and brain size. We examined the importance of c-Fos during neocortex development at 13.5, 14.5 and 16.5 days of gestation. At E14.5, neocortex thickness, apoptosis, mitosis and expression of markers along the different stages of Neural Stem Progenitor Cells (NSPCs) differentiation in c-fos-/- and wild-type mice were analyzed. A ~15% reduction in the neocortex thickness of c-fos-/- embryos was observed which correlates with a decrease in the number of differentiated cells and an increase in apoptosis at the ventricular zone. No difference in mitosis rate was observed, although the mitotic angle was predominantly vertical in c-fos-/- embryos, suggesting a reduced trend of NSPCs to differentiate. At E13.5, changes in differentiation markers start to be apparent and are still clearly observed at E16.5. A tendency of more AP-1/DNA complexes present in nuclear extracts of cerebral cortex from c-fos-/- embryos with no differences in the lipid synthesis activity was found. These results suggest that c-Fos is involved in the normal development of NSPCs by means of its AP-1 activity.
Fil: Velazquez, Fabiola Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Prucca, Cesar German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Etienne, Olivier. Laboratoire de Radiopathologie, Cea, Irsm; Francia. Université Paris Diderot - Paris 7; Francia
Fil: D'Astolfo, Diego S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Silvestre, David C.. Laboratoire de Radiopathologie, Cea, Irsm; Francia. Université Paris Diderot - Paris 7; Francia
Fil: Boussin, Francois D.. Université Paris Diderot - Paris 7; Francia. Laboratoire de Radiopathologie, Cea, Irsm; Francia
Fil: Caputto, Beatriz Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
description c-Fos is a proto-oncogene involved in diverse cellular functions. Its deregulation has been associated to abnormal development and oncogenic progression. c-fos-/- mice are viable but present a reduction in their body weight and brain size. We examined the importance of c-Fos during neocortex development at 13.5, 14.5 and 16.5 days of gestation. At E14.5, neocortex thickness, apoptosis, mitosis and expression of markers along the different stages of Neural Stem Progenitor Cells (NSPCs) differentiation in c-fos-/- and wild-type mice were analyzed. A ~15% reduction in the neocortex thickness of c-fos-/- embryos was observed which correlates with a decrease in the number of differentiated cells and an increase in apoptosis at the ventricular zone. No difference in mitosis rate was observed, although the mitotic angle was predominantly vertical in c-fos-/- embryos, suggesting a reduced trend of NSPCs to differentiate. At E13.5, changes in differentiation markers start to be apparent and are still clearly observed at E16.5. A tendency of more AP-1/DNA complexes present in nuclear extracts of cerebral cortex from c-fos-/- embryos with no differences in the lipid synthesis activity was found. These results suggest that c-Fos is involved in the normal development of NSPCs by means of its AP-1 activity.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50818
Velazquez, Fabiola Noelia; Prucca, Cesar German; Etienne, Olivier; D'Astolfo, Diego S.; Silvestre, David C.; et al.; Brain development is impaired in c-fos -/- mice; Impact Journals LLC; Oncotarget; 6; 19; 7-2015; 16883-16901
1949-2553
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50818
identifier_str_mv Velazquez, Fabiola Noelia; Prucca, Cesar German; Etienne, Olivier; D'Astolfo, Diego S.; Silvestre, David C.; et al.; Brain development is impaired in c-fos -/- mice; Impact Journals LLC; Oncotarget; 6; 19; 7-2015; 16883-16901
1949-2553
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/26143639
info:eu-repo/semantics/altIdentifier/doi/10.18632/oncotarget.4527
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Impact Journals LLC
publisher.none.fl_str_mv Impact Journals LLC
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.260194

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