Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells

Autores
Montaner, Aneley Natalia; Da Silva Santana, Themis Taynah; Schroeder, Timm; Einiker Lamas, Marcelo; Girardini Brovelli, Javier Enrique; Costa, Marcos Romualdo; Banchio, Claudia Elena
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells in vitro. Phosphatidylcholine (PtdCho)-enriched media enhances neuronal differentiation at the expense of astroglial and unspecified cells. Conversely, phosphatidylethanolamine (PtdEtn) enhances astroglial differentiation and accelerates astrocyte maturation. The ability of phospholipids to modify the fate of post-mitotic cells depends on its presence during a narrow time-window during cell differentiation and it is mediated by the selective activation of particular signaling pathways. While PtdCho-mediated effect on neuronal differentiation depends on cAMP-dependent kinase (PKA)/calcium responsive element binding protein (CREB), PtdEtn stimulates astrogliogenesis through the activation of the MEK/ERK signaling pathway. Collectively, our results provide an additional degree of plasticity in neural cell specification and further support the notion that cell differentiation is a reversible phenomenon. They also contribute to our understanding of neuronal and glial lineage specification in the central nervous system, opening up new avenues to retrieve neurogenic capacity in the brain.
Fil: Montaner, Aneley. Universidad Nacional de Rosario; Argentina. Instituto de Biología Molecular y Celular de Rosario Conicet; Argentina
Fil: Da Silva Santana, Themis Taynah. Universidade Federal do Rio Grande do Norte;Brasil; Argentina
Fil: Schroeder, Timm. Eth Zürich;
Fil: Einiker-Lamas, Marcelo. Universidade Federal do Rio de Janeiro; Brasil
Fil: Girardini, Javier. Universidad Nacional de Rosario; Argentina. Instituto de Biología Molecular y Celular de Rosario Conicet; Argentina
Fil: Costa, Marcos Romualdo. Universidade Federal do Rio Grande do Norte; Brasil
Fil: Banchio, Claudia Elena. Universidad Nacional de Rosario; Argentina. Instituto de Biología Molecular y Celular de Rosario Conicet; Argentina
Materia
PHOSPHOLIPIDS
DIFFERENTIATION
NEUROGENESIS
STEM CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92614
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/92614
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic CellsMontaner, Aneley NataliaDa Silva Santana, Themis TaynahSchroeder, TimmEiniker Lamas, MarceloGirardini Brovelli, Javier EnriqueCosta, Marcos RomualdoBanchio, Claudia ElenaPHOSPHOLIPIDSDIFFERENTIATIONNEUROGENESISSTEM CELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Hitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells in vitro. Phosphatidylcholine (PtdCho)-enriched media enhances neuronal differentiation at the expense of astroglial and unspecified cells. Conversely, phosphatidylethanolamine (PtdEtn) enhances astroglial differentiation and accelerates astrocyte maturation. The ability of phospholipids to modify the fate of post-mitotic cells depends on its presence during a narrow time-window during cell differentiation and it is mediated by the selective activation of particular signaling pathways. While PtdCho-mediated effect on neuronal differentiation depends on cAMP-dependent kinase (PKA)/calcium responsive element binding protein (CREB), PtdEtn stimulates astrogliogenesis through the activation of the MEK/ERK signaling pathway. Collectively, our results provide an additional degree of plasticity in neural cell specification and further support the notion that cell differentiation is a reversible phenomenon. They also contribute to our understanding of neuronal and glial lineage specification in the central nervous system, opening up new avenues to retrieve neurogenic capacity in the brain.Fil: Montaner, Aneley. Universidad Nacional de Rosario; Argentina. Instituto de Biología Molecular y Celular de Rosario Conicet; ArgentinaFil: Da Silva Santana, Themis Taynah. Universidade Federal do Rio Grande do Norte;Brasil; ArgentinaFil: Schroeder, Timm. Eth Zürich;Fil: Einiker-Lamas, Marcelo. Universidade Federal do Rio de Janeiro; BrasilFil: Girardini, Javier. Universidad Nacional de Rosario; Argentina. Instituto de Biología Molecular y Celular de Rosario Conicet; ArgentinaFil: Costa, Marcos Romualdo. Universidade Federal do Rio Grande do Norte; BrasilFil: Banchio, Claudia Elena. Universidad Nacional de Rosario; Argentina. Instituto de Biología Molecular y Celular de Rosario Conicet; ArgentinaNature Publishing Group2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92614Montaner, Aneley Natalia; Da Silva Santana, Themis Taynah; Schroeder, Timm; Einiker Lamas, Marcelo; Girardini Brovelli, Javier Enrique; et al.; Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-132045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-18700-4info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-017-18700-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:16Zoai:ri.conicet.gov.ar:11336/92614instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:16.59CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells
title Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells
spellingShingle Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells
Montaner, Aneley Natalia
PHOSPHOLIPIDS
DIFFERENTIATION
NEUROGENESIS
STEM CELLS
title_short Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells
title_full Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells
title_fullStr Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells
title_full_unstemmed Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells
title_sort Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells
dc.creator.none.fl_str_mv Montaner, Aneley Natalia
Da Silva Santana, Themis Taynah
Schroeder, Timm
Einiker Lamas, Marcelo
Girardini Brovelli, Javier Enrique
Costa, Marcos Romualdo
Banchio, Claudia Elena
author Montaner, Aneley Natalia
author_facet Montaner, Aneley Natalia
Da Silva Santana, Themis Taynah
Schroeder, Timm
Einiker Lamas, Marcelo
Girardini Brovelli, Javier Enrique
Costa, Marcos Romualdo
Banchio, Claudia Elena
author_role author
author2 Da Silva Santana, Themis Taynah
Schroeder, Timm
Einiker Lamas, Marcelo
Girardini Brovelli, Javier Enrique
Costa, Marcos Romualdo
Banchio, Claudia Elena
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PHOSPHOLIPIDS
DIFFERENTIATION
NEUROGENESIS
STEM CELLS
topic PHOSPHOLIPIDS
DIFFERENTIATION
NEUROGENESIS
STEM CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Hitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells in vitro. Phosphatidylcholine (PtdCho)-enriched media enhances neuronal differentiation at the expense of astroglial and unspecified cells. Conversely, phosphatidylethanolamine (PtdEtn) enhances astroglial differentiation and accelerates astrocyte maturation. The ability of phospholipids to modify the fate of post-mitotic cells depends on its presence during a narrow time-window during cell differentiation and it is mediated by the selective activation of particular signaling pathways. While PtdCho-mediated effect on neuronal differentiation depends on cAMP-dependent kinase (PKA)/calcium responsive element binding protein (CREB), PtdEtn stimulates astrogliogenesis through the activation of the MEK/ERK signaling pathway. Collectively, our results provide an additional degree of plasticity in neural cell specification and further support the notion that cell differentiation is a reversible phenomenon. They also contribute to our understanding of neuronal and glial lineage specification in the central nervous system, opening up new avenues to retrieve neurogenic capacity in the brain.
Fil: Montaner, Aneley. Universidad Nacional de Rosario; Argentina. Instituto de Biología Molecular y Celular de Rosario Conicet; Argentina
Fil: Da Silva Santana, Themis Taynah. Universidade Federal do Rio Grande do Norte;Brasil; Argentina
Fil: Schroeder, Timm. Eth Zürich;
Fil: Einiker-Lamas, Marcelo. Universidade Federal do Rio de Janeiro; Brasil
Fil: Girardini, Javier. Universidad Nacional de Rosario; Argentina. Instituto de Biología Molecular y Celular de Rosario Conicet; Argentina
Fil: Costa, Marcos Romualdo. Universidade Federal do Rio Grande do Norte; Brasil
Fil: Banchio, Claudia Elena. Universidad Nacional de Rosario; Argentina. Instituto de Biología Molecular y Celular de Rosario Conicet; Argentina
description Hitherto, the known mechanisms underpinning cell-fate specification act on neural progenitors, affecting their commitment to generate neuron or glial cells. Here, we show that particular phospholipids supplemented in the culture media modify the commitment of post-mitotic neural cells in vitro. Phosphatidylcholine (PtdCho)-enriched media enhances neuronal differentiation at the expense of astroglial and unspecified cells. Conversely, phosphatidylethanolamine (PtdEtn) enhances astroglial differentiation and accelerates astrocyte maturation. The ability of phospholipids to modify the fate of post-mitotic cells depends on its presence during a narrow time-window during cell differentiation and it is mediated by the selective activation of particular signaling pathways. While PtdCho-mediated effect on neuronal differentiation depends on cAMP-dependent kinase (PKA)/calcium responsive element binding protein (CREB), PtdEtn stimulates astrogliogenesis through the activation of the MEK/ERK signaling pathway. Collectively, our results provide an additional degree of plasticity in neural cell specification and further support the notion that cell differentiation is a reversible phenomenon. They also contribute to our understanding of neuronal and glial lineage specification in the central nervous system, opening up new avenues to retrieve neurogenic capacity in the brain.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92614
Montaner, Aneley Natalia; Da Silva Santana, Themis Taynah; Schroeder, Timm; Einiker Lamas, Marcelo; Girardini Brovelli, Javier Enrique; et al.; Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-13
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92614
identifier_str_mv Montaner, Aneley Natalia; Da Silva Santana, Themis Taynah; Schroeder, Timm; Einiker Lamas, Marcelo; Girardini Brovelli, Javier Enrique; et al.; Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells; Nature Publishing Group; Scientific Reports; 8; 1; 12-2018; 1-13
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-18700-4
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-017-18700-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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