From the epididymis to the egg: participation of CRISP proteins in mammalian fertilization

Autores
Da Ros, Vanina Gabriela; Weigel Muñoz, Mariana; Battistone, Maria Agustina; Brukman, Nicolás Gastón; Carvajal, Guillermo; Curci, Ludmila; Gómez Elías, Matías Daniel; Cohen, Debora Juana; Cuasnicu, Patricia Sara
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mammalian fertilization is a complex process that involves different steps of interaction between the male and female gametes. In spite of its relevance, the molecular mechanisms underlying this process still remain to be elucidated. The present review describes the contribution of our laboratory to the understanding of mammalian fertilization by using CRISP (Cysteine-RIch Secretory Proteins) as model molecules. Substantial evidence obtained using in vitro assays and knockout models shows that epididymal CRISP1 associates with the sperm surface with two different affinities during maturation and participates in the regulation of signaling pathways during capacitation as well as in both sperm-zona pellucida interaction and gamete fusion. These observations can be extended to humans as judged by our findings showing that the human homologue of the rodent protein (hCRISP1) is also involved in both stages of fertilization. Evidence supports that other members of the CRISP family secreted in the testes (CRISP2), epididymis (CRISP3-4) or during ejaculation (CRISP3) are also involved in sperm-egg interaction, supporting the existence of a functional redundancy and cooperation between homologue proteins to ensure the success of fertilization. Together, our observations indicate that CRISP proteins escort sperm along their transit through both the male and female reproductive tracts. We believe these results not only contribute to a better mechanistic understanding of fertilization but also support CRISP proteins as excellent candidates for future research on infertility and contraception.
Fil: Da Ros, Vanina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Battistone, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Brukman, Nicolás Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Carvajal, Guillermo. Fundacion de Instituto de Biologia y Medicina Experimental; Argentina
Fil: Curci, Ludmila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Gómez Elías, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Materia
SPERM
EPIDIDYMAL PROTEINS
FERTILIZATION
CRISP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3059
Acceder
id CONICETDig_4d476fe5e0a236ab6e5e18ae8ecbfa43
oai_identifier_str oai:ri.conicet.gov.ar:11336/3059
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling From the epididymis to the egg: participation of CRISP proteins in mammalian fertilizationDa Ros, Vanina GabrielaWeigel Muñoz, MarianaBattistone, Maria AgustinaBrukman, Nicolás GastónCarvajal, GuillermoCurci, LudmilaGómez Elías, Matías DanielCohen, Debora JuanaCuasnicu, Patricia SaraSPERMEPIDIDYMAL PROTEINSFERTILIZATIONCRISPhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mammalian fertilization is a complex process that involves different steps of interaction between the male and female gametes. In spite of its relevance, the molecular mechanisms underlying this process still remain to be elucidated. The present review describes the contribution of our laboratory to the understanding of mammalian fertilization by using CRISP (Cysteine-RIch Secretory Proteins) as model molecules. Substantial evidence obtained using in vitro assays and knockout models shows that epididymal CRISP1 associates with the sperm surface with two different affinities during maturation and participates in the regulation of signaling pathways during capacitation as well as in both sperm-zona pellucida interaction and gamete fusion. These observations can be extended to humans as judged by our findings showing that the human homologue of the rodent protein (hCRISP1) is also involved in both stages of fertilization. Evidence supports that other members of the CRISP family secreted in the testes (CRISP2), epididymis (CRISP3-4) or during ejaculation (CRISP3) are also involved in sperm-egg interaction, supporting the existence of a functional redundancy and cooperation between homologue proteins to ensure the success of fertilization. Together, our observations indicate that CRISP proteins escort sperm along their transit through both the male and female reproductive tracts. We believe these results not only contribute to a better mechanistic understanding of fertilization but also support CRISP proteins as excellent candidates for future research on infertility and contraception.Fil: Da Ros, Vanina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Battistone, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Brukman, Nicolás Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Carvajal, Guillermo. Fundacion de Instituto de Biologia y Medicina Experimental; ArgentinaFil: Curci, Ludmila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Gómez Elías, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaNature Publishing Group2015-06-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3059Da Ros, Vanina Gabriela; Weigel Muñoz, Mariana; Battistone, Maria Agustina; Brukman, Nicolás Gastón; Carvajal, Guillermo; et al.; From the epididymis to the egg: participation of CRISP proteins in mammalian fertilization; Nature Publishing Group; Asian Journal Of Andrology; 17; 5; 26-6-2015; 711-7151008-682Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4577577/info:eu-repo/semantics/altIdentifier/doi/doi:10.4103/1008-682X.155769info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/articles/PMC4577577;jsessionid=PglLxTP0bGwgt08FIP57.0info:eu-repo/semantics/altIdentifier/url/http://www.ajandrology.com/article.asp?issn=1008-682X;year=2015;volume=17;issue=5;spage=711;epage=715;aulast=Dainfo:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)https://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:40:52Zoai:ri.conicet.gov.ar:11336/3059instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:40:52.488CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv From the epididymis to the egg: participation of CRISP proteins in mammalian fertilization
title From the epididymis to the egg: participation of CRISP proteins in mammalian fertilization
spellingShingle From the epididymis to the egg: participation of CRISP proteins in mammalian fertilization
Da Ros, Vanina Gabriela
SPERM
EPIDIDYMAL PROTEINS
FERTILIZATION
CRISP
title_short From the epididymis to the egg: participation of CRISP proteins in mammalian fertilization
title_full From the epididymis to the egg: participation of CRISP proteins in mammalian fertilization
title_fullStr From the epididymis to the egg: participation of CRISP proteins in mammalian fertilization
title_full_unstemmed From the epididymis to the egg: participation of CRISP proteins in mammalian fertilization
title_sort From the epididymis to the egg: participation of CRISP proteins in mammalian fertilization
dc.creator.none.fl_str_mv Da Ros, Vanina Gabriela
Weigel Muñoz, Mariana
Battistone, Maria Agustina
Brukman, Nicolás Gastón
Carvajal, Guillermo
Curci, Ludmila
Gómez Elías, Matías Daniel
Cohen, Debora Juana
Cuasnicu, Patricia Sara
author Da Ros, Vanina Gabriela
author_facet Da Ros, Vanina Gabriela
Weigel Muñoz, Mariana
Battistone, Maria Agustina
Brukman, Nicolás Gastón
Carvajal, Guillermo
Curci, Ludmila
Gómez Elías, Matías Daniel
Cohen, Debora Juana
Cuasnicu, Patricia Sara
author_role author
author2 Weigel Muñoz, Mariana
Battistone, Maria Agustina
Brukman, Nicolás Gastón
Carvajal, Guillermo
Curci, Ludmila
Gómez Elías, Matías Daniel
Cohen, Debora Juana
Cuasnicu, Patricia Sara
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SPERM
EPIDIDYMAL PROTEINS
FERTILIZATION
CRISP
topic SPERM
EPIDIDYMAL PROTEINS
FERTILIZATION
CRISP
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mammalian fertilization is a complex process that involves different steps of interaction between the male and female gametes. In spite of its relevance, the molecular mechanisms underlying this process still remain to be elucidated. The present review describes the contribution of our laboratory to the understanding of mammalian fertilization by using CRISP (Cysteine-RIch Secretory Proteins) as model molecules. Substantial evidence obtained using in vitro assays and knockout models shows that epididymal CRISP1 associates with the sperm surface with two different affinities during maturation and participates in the regulation of signaling pathways during capacitation as well as in both sperm-zona pellucida interaction and gamete fusion. These observations can be extended to humans as judged by our findings showing that the human homologue of the rodent protein (hCRISP1) is also involved in both stages of fertilization. Evidence supports that other members of the CRISP family secreted in the testes (CRISP2), epididymis (CRISP3-4) or during ejaculation (CRISP3) are also involved in sperm-egg interaction, supporting the existence of a functional redundancy and cooperation between homologue proteins to ensure the success of fertilization. Together, our observations indicate that CRISP proteins escort sperm along their transit through both the male and female reproductive tracts. We believe these results not only contribute to a better mechanistic understanding of fertilization but also support CRISP proteins as excellent candidates for future research on infertility and contraception.
Fil: Da Ros, Vanina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Battistone, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Brukman, Nicolás Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Carvajal, Guillermo. Fundacion de Instituto de Biologia y Medicina Experimental; Argentina
Fil: Curci, Ludmila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Gómez Elías, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
description Mammalian fertilization is a complex process that involves different steps of interaction between the male and female gametes. In spite of its relevance, the molecular mechanisms underlying this process still remain to be elucidated. The present review describes the contribution of our laboratory to the understanding of mammalian fertilization by using CRISP (Cysteine-RIch Secretory Proteins) as model molecules. Substantial evidence obtained using in vitro assays and knockout models shows that epididymal CRISP1 associates with the sperm surface with two different affinities during maturation and participates in the regulation of signaling pathways during capacitation as well as in both sperm-zona pellucida interaction and gamete fusion. These observations can be extended to humans as judged by our findings showing that the human homologue of the rodent protein (hCRISP1) is also involved in both stages of fertilization. Evidence supports that other members of the CRISP family secreted in the testes (CRISP2), epididymis (CRISP3-4) or during ejaculation (CRISP3) are also involved in sperm-egg interaction, supporting the existence of a functional redundancy and cooperation between homologue proteins to ensure the success of fertilization. Together, our observations indicate that CRISP proteins escort sperm along their transit through both the male and female reproductive tracts. We believe these results not only contribute to a better mechanistic understanding of fertilization but also support CRISP proteins as excellent candidates for future research on infertility and contraception.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-26
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3059
Da Ros, Vanina Gabriela; Weigel Muñoz, Mariana; Battistone, Maria Agustina; Brukman, Nicolás Gastón; Carvajal, Guillermo; et al.; From the epididymis to the egg: participation of CRISP proteins in mammalian fertilization; Nature Publishing Group; Asian Journal Of Andrology; 17; 5; 26-6-2015; 711-715
1008-682X
url http://hdl.handle.net/11336/3059
identifier_str_mv Da Ros, Vanina Gabriela; Weigel Muñoz, Mariana; Battistone, Maria Agustina; Brukman, Nicolás Gastón; Carvajal, Guillermo; et al.; From the epididymis to the egg: participation of CRISP proteins in mammalian fertilization; Nature Publishing Group; Asian Journal Of Andrology; 17; 5; 26-6-2015; 711-715
1008-682X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4577577/
info:eu-repo/semantics/altIdentifier/doi/doi:10.4103/1008-682X.155769
info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/articles/PMC4577577;jsessionid=PglLxTP0bGwgt08FIP57.0
info:eu-repo/semantics/altIdentifier/url/http://www.ajandrology.com/article.asp?issn=1008-682X;year=2015;volume=17;issue=5;spage=711;epage=715;aulast=Da
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1848597536924762112
score 13.24909

Publicaciones similares