Dedifferentiation of prostate smooth muscle cells in response to bacterial LPS

Autores
Leimgruber, Carolina; Quintar, Amado Alfredo; Sosa, Liliana del Valle; García, Luciana Noemí; Figueredo, Carlos Mauricio; Maldonado, Cristina Alicia
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Prostate smooth muscle cells (SMCs) are strongly involved in the development and progression of benign prostatic hyperplasia and prostate cancer. However, their participation in prostatitis has not been completely elucidated. Thus, we aimed to characterize the response of normal SMC to bacterial lipopolysaccharide (LPS). Methods Primary prostate SMCs from normal rats were stimulated with LPS (0.1, 1, or 10Âμg/ml) for 24 or 48hr. The phenotype was evaluated by electron microscopy, immunofluorescence, and Western blot of SMCα-actin (ACTA2), calponin, vimentin, and tenascin-C, while the innate immune response was assessed by immunodetection of TLR4, CD14, and nuclear NF-κB. The secretion of TNFα and IL6 was determined using ELISA. Results Bacterial LPS induces SMCs to develop a secretory phenotype including dilated rough endoplasmic reticulum cisternae with well-developed Golgi complexes. Furthermore, SMCs displayed a decrease in ACTA2 and calponin, and an increase in vimentin levels after LPS challenge. The co-expression of ACTA2 and vimentin, together with the induction of tenascin-C expression indicate that a myofibroblastic-like phenotype was induced by the endotoxin. Moreover, LPS elicited a TLR4 increase, with a peak in NF-κB activation occurring after 10min of treatment. Finally, LPS stimulated the secretion of IL6 and TNFα. ConclusionS Prostate SMCs are capable of responding to LPS in vitro by dedifferentiating from a contractile to a miofibroblastic-like phenotype and secreting cytokines, with the TLR4 signaling pathway being involved in this response. In this way, prostate SMCs may contribute to the pathophysiology of inflammatory diseases by modifying the epithelial-stromal interactions.
Fil: Leimgruber, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Quintar, Amado Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Sosa, Liliana del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: García, Luciana Noemí. Universidad Nacional de Córdoba; Argentina. Centro de Investigación de la Fundación Repro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Figueredo, Carlos Mauricio. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Maldonado, Cristina Alicia. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Materia
DEDIFFERENTIATION
INNATE IMMUNE RESPONSE
LPS
SMOOTH MUSCLE CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194361
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/194361
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dedifferentiation of prostate smooth muscle cells in response to bacterial LPSLeimgruber, CarolinaQuintar, Amado AlfredoSosa, Liliana del ValleGarcía, Luciana NoemíFigueredo, Carlos MauricioMaldonado, Cristina AliciaDEDIFFERENTIATIONINNATE IMMUNE RESPONSELPSSMOOTH MUSCLE CELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Prostate smooth muscle cells (SMCs) are strongly involved in the development and progression of benign prostatic hyperplasia and prostate cancer. However, their participation in prostatitis has not been completely elucidated. Thus, we aimed to characterize the response of normal SMC to bacterial lipopolysaccharide (LPS). Methods Primary prostate SMCs from normal rats were stimulated with LPS (0.1, 1, or 10Âμg/ml) for 24 or 48hr. The phenotype was evaluated by electron microscopy, immunofluorescence, and Western blot of SMCα-actin (ACTA2), calponin, vimentin, and tenascin-C, while the innate immune response was assessed by immunodetection of TLR4, CD14, and nuclear NF-κB. The secretion of TNFα and IL6 was determined using ELISA. Results Bacterial LPS induces SMCs to develop a secretory phenotype including dilated rough endoplasmic reticulum cisternae with well-developed Golgi complexes. Furthermore, SMCs displayed a decrease in ACTA2 and calponin, and an increase in vimentin levels after LPS challenge. The co-expression of ACTA2 and vimentin, together with the induction of tenascin-C expression indicate that a myofibroblastic-like phenotype was induced by the endotoxin. Moreover, LPS elicited a TLR4 increase, with a peak in NF-κB activation occurring after 10min of treatment. Finally, LPS stimulated the secretion of IL6 and TNFα. ConclusionS Prostate SMCs are capable of responding to LPS in vitro by dedifferentiating from a contractile to a miofibroblastic-like phenotype and secreting cytokines, with the TLR4 signaling pathway being involved in this response. In this way, prostate SMCs may contribute to the pathophysiology of inflammatory diseases by modifying the epithelial-stromal interactions.Fil: Leimgruber, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Quintar, Amado Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Sosa, Liliana del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: García, Luciana Noemí. Universidad Nacional de Córdoba; Argentina. Centro de Investigación de la Fundación Repro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Figueredo, Carlos Mauricio. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Maldonado, Cristina Alicia. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaWiley-liss, div John Wiley & Sons Inc.2011-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194361Leimgruber, Carolina; Quintar, Amado Alfredo; Sosa, Liliana del Valle; García, Luciana Noemí; Figueredo, Carlos Mauricio; et al.; Dedifferentiation of prostate smooth muscle cells in response to bacterial LPS; Wiley-liss, div John Wiley & Sons Inc.; Prostate; 71; 10; 7-2011; 1097-11070270-41371097-0045CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/pros.21322info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/pros.21322info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:45:36Zoai:ri.conicet.gov.ar:11336/194361instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:45:37.074CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dedifferentiation of prostate smooth muscle cells in response to bacterial LPS
title Dedifferentiation of prostate smooth muscle cells in response to bacterial LPS
spellingShingle Dedifferentiation of prostate smooth muscle cells in response to bacterial LPS
Leimgruber, Carolina
DEDIFFERENTIATION
INNATE IMMUNE RESPONSE
LPS
SMOOTH MUSCLE CELLS
title_short Dedifferentiation of prostate smooth muscle cells in response to bacterial LPS
title_full Dedifferentiation of prostate smooth muscle cells in response to bacterial LPS
title_fullStr Dedifferentiation of prostate smooth muscle cells in response to bacterial LPS
title_full_unstemmed Dedifferentiation of prostate smooth muscle cells in response to bacterial LPS
title_sort Dedifferentiation of prostate smooth muscle cells in response to bacterial LPS
dc.creator.none.fl_str_mv Leimgruber, Carolina
Quintar, Amado Alfredo
Sosa, Liliana del Valle
García, Luciana Noemí
Figueredo, Carlos Mauricio
Maldonado, Cristina Alicia
author Leimgruber, Carolina
author_facet Leimgruber, Carolina
Quintar, Amado Alfredo
Sosa, Liliana del Valle
García, Luciana Noemí
Figueredo, Carlos Mauricio
Maldonado, Cristina Alicia
author_role author
author2 Quintar, Amado Alfredo
Sosa, Liliana del Valle
García, Luciana Noemí
Figueredo, Carlos Mauricio
Maldonado, Cristina Alicia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv DEDIFFERENTIATION
INNATE IMMUNE RESPONSE
LPS
SMOOTH MUSCLE CELLS
topic DEDIFFERENTIATION
INNATE IMMUNE RESPONSE
LPS
SMOOTH MUSCLE CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Prostate smooth muscle cells (SMCs) are strongly involved in the development and progression of benign prostatic hyperplasia and prostate cancer. However, their participation in prostatitis has not been completely elucidated. Thus, we aimed to characterize the response of normal SMC to bacterial lipopolysaccharide (LPS). Methods Primary prostate SMCs from normal rats were stimulated with LPS (0.1, 1, or 10Âμg/ml) for 24 or 48hr. The phenotype was evaluated by electron microscopy, immunofluorescence, and Western blot of SMCα-actin (ACTA2), calponin, vimentin, and tenascin-C, while the innate immune response was assessed by immunodetection of TLR4, CD14, and nuclear NF-κB. The secretion of TNFα and IL6 was determined using ELISA. Results Bacterial LPS induces SMCs to develop a secretory phenotype including dilated rough endoplasmic reticulum cisternae with well-developed Golgi complexes. Furthermore, SMCs displayed a decrease in ACTA2 and calponin, and an increase in vimentin levels after LPS challenge. The co-expression of ACTA2 and vimentin, together with the induction of tenascin-C expression indicate that a myofibroblastic-like phenotype was induced by the endotoxin. Moreover, LPS elicited a TLR4 increase, with a peak in NF-κB activation occurring after 10min of treatment. Finally, LPS stimulated the secretion of IL6 and TNFα. ConclusionS Prostate SMCs are capable of responding to LPS in vitro by dedifferentiating from a contractile to a miofibroblastic-like phenotype and secreting cytokines, with the TLR4 signaling pathway being involved in this response. In this way, prostate SMCs may contribute to the pathophysiology of inflammatory diseases by modifying the epithelial-stromal interactions.
Fil: Leimgruber, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Quintar, Amado Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Sosa, Liliana del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: García, Luciana Noemí. Universidad Nacional de Córdoba; Argentina. Centro de Investigación de la Fundación Repro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Figueredo, Carlos Mauricio. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Maldonado, Cristina Alicia. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
description Prostate smooth muscle cells (SMCs) are strongly involved in the development and progression of benign prostatic hyperplasia and prostate cancer. However, their participation in prostatitis has not been completely elucidated. Thus, we aimed to characterize the response of normal SMC to bacterial lipopolysaccharide (LPS). Methods Primary prostate SMCs from normal rats were stimulated with LPS (0.1, 1, or 10Âμg/ml) for 24 or 48hr. The phenotype was evaluated by electron microscopy, immunofluorescence, and Western blot of SMCα-actin (ACTA2), calponin, vimentin, and tenascin-C, while the innate immune response was assessed by immunodetection of TLR4, CD14, and nuclear NF-κB. The secretion of TNFα and IL6 was determined using ELISA. Results Bacterial LPS induces SMCs to develop a secretory phenotype including dilated rough endoplasmic reticulum cisternae with well-developed Golgi complexes. Furthermore, SMCs displayed a decrease in ACTA2 and calponin, and an increase in vimentin levels after LPS challenge. The co-expression of ACTA2 and vimentin, together with the induction of tenascin-C expression indicate that a myofibroblastic-like phenotype was induced by the endotoxin. Moreover, LPS elicited a TLR4 increase, with a peak in NF-κB activation occurring after 10min of treatment. Finally, LPS stimulated the secretion of IL6 and TNFα. ConclusionS Prostate SMCs are capable of responding to LPS in vitro by dedifferentiating from a contractile to a miofibroblastic-like phenotype and secreting cytokines, with the TLR4 signaling pathway being involved in this response. In this way, prostate SMCs may contribute to the pathophysiology of inflammatory diseases by modifying the epithelial-stromal interactions.
publishDate 2011
dc.date.none.fl_str_mv 2011-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194361
Leimgruber, Carolina; Quintar, Amado Alfredo; Sosa, Liliana del Valle; García, Luciana Noemí; Figueredo, Carlos Mauricio; et al.; Dedifferentiation of prostate smooth muscle cells in response to bacterial LPS; Wiley-liss, div John Wiley & Sons Inc.; Prostate; 71; 10; 7-2011; 1097-1107
0270-4137
1097-0045
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194361
identifier_str_mv Leimgruber, Carolina; Quintar, Amado Alfredo; Sosa, Liliana del Valle; García, Luciana Noemí; Figueredo, Carlos Mauricio; et al.; Dedifferentiation of prostate smooth muscle cells in response to bacterial LPS; Wiley-liss, div John Wiley & Sons Inc.; Prostate; 71; 10; 7-2011; 1097-1107
0270-4137
1097-0045
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/pros.21322
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/pros.21322
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
publisher.none.fl_str_mv Wiley-liss, div John Wiley & Sons Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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