Novel Insights Into Basic Research: Exploring Molecular Effects Of Trans-Cinnamaldehyde And Eugenol On α7 And Muscle Acetylcholine Receptors Via Electrophysiological Assessment

Autores
Hernando, Guillermina Silvana; Chrestia, Juan Facundo; Leda, Sol; Bouzat, Cecilia Beatriz
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Naturally occurring bioactive compound have been used in tradi- tional medicine for the treatment of various diseases for many cen- turies. In modern times, there has been a renewed interest in nat- ural compounds and essential oils for their medicinal properties. In recent years, it has been demonstrated that many of these natural compounds act on neurotransmitter receptors, including Cys-loop receptors such as the nicotinic acetylcholine receptor (nAChR). nA- ChRs form a family of ACh-gated ion channels found in the central and peripheral nervous systems, involved in processes like muscle contraction, memory, and attention. In this project, we assessed the molecular-level effects of trans-cinnamaldehyde (TC) and eugenol (EGN) -the two naturally occurring phenylpropanoids present in Cinnamomum oil- on two types of mammalians nAChR, which are involved in several pathological disorders. As TC and EGN are mul- titarget drug, there is a need to establish the molecular mechanisms by which they may exert therapeutic as well as adverse effects. Through single-channel recordings, we observed that TC exerts a negative modulatory effect on both α7 and muscular nAChR. On the one hand, TC leads to a marked reduction of α7 activity by decreas- ing the frequency of activation episodes without causing changes in amplitude and in the open durations. On the other hand, recordings from muscular nAChR exhibit a concentration-dependent reduction in open channel duration within the micromolar range, induced by both TC and EGN. This change is accompanied by a shift towards shorter durations in the main closed component. The modulation of nAChRs is of pharmacological relevance and should be considered in the evaluation of the potential therapeutic uses of TC and EGN as. Our findings offer insights into how natural compounds influence Cys-loop receptors, which remain unexplored and are important tar- gets in various therapeutic approaches
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Chrestia, Juan Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Leda, Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal)
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
bioactive compound
α7 nicotinic acetylcholine receptor
trans-cinnamaldehyde
muscular nAChR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/236310
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/236310
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Novel Insights Into Basic Research: Exploring Molecular Effects Of Trans-Cinnamaldehyde And Eugenol On α7 And Muscle Acetylcholine Receptors Via Electrophysiological AssessmentHernando, Guillermina SilvanaChrestia, Juan FacundoLeda, SolBouzat, Cecilia Beatrizbioactive compoundα7 nicotinic acetylcholine receptortrans-cinnamaldehydemuscular nAChRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Naturally occurring bioactive compound have been used in tradi- tional medicine for the treatment of various diseases for many cen- turies. In modern times, there has been a renewed interest in nat- ural compounds and essential oils for their medicinal properties. In recent years, it has been demonstrated that many of these natural compounds act on neurotransmitter receptors, including Cys-loop receptors such as the nicotinic acetylcholine receptor (nAChR). nA- ChRs form a family of ACh-gated ion channels found in the central and peripheral nervous systems, involved in processes like muscle contraction, memory, and attention. In this project, we assessed the molecular-level effects of trans-cinnamaldehyde (TC) and eugenol (EGN) -the two naturally occurring phenylpropanoids present in Cinnamomum oil- on two types of mammalians nAChR, which are involved in several pathological disorders. As TC and EGN are mul- titarget drug, there is a need to establish the molecular mechanisms by which they may exert therapeutic as well as adverse effects. Through single-channel recordings, we observed that TC exerts a negative modulatory effect on both α7 and muscular nAChR. On the one hand, TC leads to a marked reduction of α7 activity by decreas- ing the frequency of activation episodes without causing changes in amplitude and in the open durations. On the other hand, recordings from muscular nAChR exhibit a concentration-dependent reduction in open channel duration within the micromolar range, induced by both TC and EGN. This change is accompanied by a shift towards shorter durations in the main closed component. The modulation of nAChRs is of pharmacological relevance and should be considered in the evaluation of the potential therapeutic uses of TC and EGN as. Our findings offer insights into how natural compounds influence Cys-loop receptors, which remain unexplored and are important tar- gets in various therapeutic approachesFil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Chrestia, Juan Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Leda, Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaReunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal)Mar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de BiologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista MedicinaLuthy, Isabel AliciaPerez Martinez, Silvina LauraSimonovich, VenturaPinto, Gabriel2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/236310Novel Insights Into Basic Research: Exploring Molecular Effects Of Trans-Cinnamaldehyde And Eugenol On α7 And Muscle Acetylcholine Receptors Via Electrophysiological Assessment; Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal); Mar del Plata; Argentina; 2023; 213-2131669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2020/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:05Zoai:ri.conicet.gov.ar:11336/236310instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:06.047CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Novel Insights Into Basic Research: Exploring Molecular Effects Of Trans-Cinnamaldehyde And Eugenol On α7 And Muscle Acetylcholine Receptors Via Electrophysiological Assessment
title Novel Insights Into Basic Research: Exploring Molecular Effects Of Trans-Cinnamaldehyde And Eugenol On α7 And Muscle Acetylcholine Receptors Via Electrophysiological Assessment
spellingShingle Novel Insights Into Basic Research: Exploring Molecular Effects Of Trans-Cinnamaldehyde And Eugenol On α7 And Muscle Acetylcholine Receptors Via Electrophysiological Assessment
Hernando, Guillermina Silvana
bioactive compound
α7 nicotinic acetylcholine receptor
trans-cinnamaldehyde
muscular nAChR
title_short Novel Insights Into Basic Research: Exploring Molecular Effects Of Trans-Cinnamaldehyde And Eugenol On α7 And Muscle Acetylcholine Receptors Via Electrophysiological Assessment
title_full Novel Insights Into Basic Research: Exploring Molecular Effects Of Trans-Cinnamaldehyde And Eugenol On α7 And Muscle Acetylcholine Receptors Via Electrophysiological Assessment
title_fullStr Novel Insights Into Basic Research: Exploring Molecular Effects Of Trans-Cinnamaldehyde And Eugenol On α7 And Muscle Acetylcholine Receptors Via Electrophysiological Assessment
title_full_unstemmed Novel Insights Into Basic Research: Exploring Molecular Effects Of Trans-Cinnamaldehyde And Eugenol On α7 And Muscle Acetylcholine Receptors Via Electrophysiological Assessment
title_sort Novel Insights Into Basic Research: Exploring Molecular Effects Of Trans-Cinnamaldehyde And Eugenol On α7 And Muscle Acetylcholine Receptors Via Electrophysiological Assessment
dc.creator.none.fl_str_mv Hernando, Guillermina Silvana
Chrestia, Juan Facundo
Leda, Sol
Bouzat, Cecilia Beatriz
author Hernando, Guillermina Silvana
author_facet Hernando, Guillermina Silvana
Chrestia, Juan Facundo
Leda, Sol
Bouzat, Cecilia Beatriz
author_role author
author2 Chrestia, Juan Facundo
Leda, Sol
Bouzat, Cecilia Beatriz
author2_role author
author
author
dc.contributor.none.fl_str_mv Luthy, Isabel Alicia
Perez Martinez, Silvina Laura
Simonovich, Ventura
Pinto, Gabriel
dc.subject.none.fl_str_mv bioactive compound
α7 nicotinic acetylcholine receptor
trans-cinnamaldehyde
muscular nAChR
topic bioactive compound
α7 nicotinic acetylcholine receptor
trans-cinnamaldehyde
muscular nAChR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Naturally occurring bioactive compound have been used in tradi- tional medicine for the treatment of various diseases for many cen- turies. In modern times, there has been a renewed interest in nat- ural compounds and essential oils for their medicinal properties. In recent years, it has been demonstrated that many of these natural compounds act on neurotransmitter receptors, including Cys-loop receptors such as the nicotinic acetylcholine receptor (nAChR). nA- ChRs form a family of ACh-gated ion channels found in the central and peripheral nervous systems, involved in processes like muscle contraction, memory, and attention. In this project, we assessed the molecular-level effects of trans-cinnamaldehyde (TC) and eugenol (EGN) -the two naturally occurring phenylpropanoids present in Cinnamomum oil- on two types of mammalians nAChR, which are involved in several pathological disorders. As TC and EGN are mul- titarget drug, there is a need to establish the molecular mechanisms by which they may exert therapeutic as well as adverse effects. Through single-channel recordings, we observed that TC exerts a negative modulatory effect on both α7 and muscular nAChR. On the one hand, TC leads to a marked reduction of α7 activity by decreas- ing the frequency of activation episodes without causing changes in amplitude and in the open durations. On the other hand, recordings from muscular nAChR exhibit a concentration-dependent reduction in open channel duration within the micromolar range, induced by both TC and EGN. This change is accompanied by a shift towards shorter durations in the main closed component. The modulation of nAChRs is of pharmacological relevance and should be considered in the evaluation of the potential therapeutic uses of TC and EGN as. Our findings offer insights into how natural compounds influence Cys-loop receptors, which remain unexplored and are important tar- gets in various therapeutic approaches
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Chrestia, Juan Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Leda, Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal)
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description Naturally occurring bioactive compound have been used in tradi- tional medicine for the treatment of various diseases for many cen- turies. In modern times, there has been a renewed interest in nat- ural compounds and essential oils for their medicinal properties. In recent years, it has been demonstrated that many of these natural compounds act on neurotransmitter receptors, including Cys-loop receptors such as the nicotinic acetylcholine receptor (nAChR). nA- ChRs form a family of ACh-gated ion channels found in the central and peripheral nervous systems, involved in processes like muscle contraction, memory, and attention. In this project, we assessed the molecular-level effects of trans-cinnamaldehyde (TC) and eugenol (EGN) -the two naturally occurring phenylpropanoids present in Cinnamomum oil- on two types of mammalians nAChR, which are involved in several pathological disorders. As TC and EGN are mul- titarget drug, there is a need to establish the molecular mechanisms by which they may exert therapeutic as well as adverse effects. Through single-channel recordings, we observed that TC exerts a negative modulatory effect on both α7 and muscular nAChR. On the one hand, TC leads to a marked reduction of α7 activity by decreas- ing the frequency of activation episodes without causing changes in amplitude and in the open durations. On the other hand, recordings from muscular nAChR exhibit a concentration-dependent reduction in open channel duration within the micromolar range, induced by both TC and EGN. This change is accompanied by a shift towards shorter durations in the main closed component. The modulation of nAChRs is of pharmacological relevance and should be considered in the evaluation of the potential therapeutic uses of TC and EGN as. Our findings offer insights into how natural compounds influence Cys-loop receptors, which remain unexplored and are important tar- gets in various therapeutic approaches
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/236310
Novel Insights Into Basic Research: Exploring Molecular Effects Of Trans-Cinnamaldehyde And Eugenol On α7 And Muscle Acetylcholine Receptors Via Electrophysiological Assessment; Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal); Mar del Plata; Argentina; 2023; 213-213
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/236310
identifier_str_mv Novel Insights Into Basic Research: Exploring Molecular Effects Of Trans-Cinnamaldehyde And Eugenol On α7 And Muscle Acetylcholine Receptors Via Electrophysiological Assessment; Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal); Mar del Plata; Argentina; 2023; 213-213
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2020/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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