In vitro evaluation of synergistic activity between ciprofloxacin and broad snouted caiman serum against Escherichia coli

Autores
Siroski, Pablo Ariel; Russi, N. B.; Ortega, Hugo Hector; Formentini, Enrique Antonio
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The in vitro synergistic activity between ciprofloxacin and serum of broad snouted caiman on Escherichia coli was studied. The estimated MIC value of ciprofloxacin was 0.0188 Μg/ml, and two assays of kill curve during 5 hours were performed: the first one in a standard culture medium and the second one in the presence of caiman serum. Different concentrations of ciprofloxacin were tested. Ciprofloxacin showed higher values of bacterial elimination rate in the presence of caiman serum in all concentrations tested. The combined activity of sub-inhibitory concentrations of ciprofloxacin and the humoral immune factors present in caiman serum determined an increase in the bacterial elimination observed in this assay. We suggest that the antibacterial activity of complement and natural antibodies present in caiman serum, which can bind to both Gram-negative and Gram-positive bacteria and acting through the classical complement pathway, can inhibit bacterial growth of Escherichia coli by lysis.
Fil: Siroski, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina
Fil: Russi, N. B.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Formentini, Enrique Antonio. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Materia
Caiman Serum
Ciprofloxacin
Escherichia Coli
Synergism
Zootechnical Interest
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39587
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro evaluation of synergistic activity between ciprofloxacin and broad snouted caiman serum against Escherichia coliSiroski, Pablo ArielRussi, N. B.Ortega, Hugo HectorFormentini, Enrique AntonioCaiman SerumCiprofloxacinEscherichia ColiSynergismZootechnical Interesthttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The in vitro synergistic activity between ciprofloxacin and serum of broad snouted caiman on Escherichia coli was studied. The estimated MIC value of ciprofloxacin was 0.0188 Μg/ml, and two assays of kill curve during 5 hours were performed: the first one in a standard culture medium and the second one in the presence of caiman serum. Different concentrations of ciprofloxacin were tested. Ciprofloxacin showed higher values of bacterial elimination rate in the presence of caiman serum in all concentrations tested. The combined activity of sub-inhibitory concentrations of ciprofloxacin and the humoral immune factors present in caiman serum determined an increase in the bacterial elimination observed in this assay. We suggest that the antibacterial activity of complement and natural antibodies present in caiman serum, which can bind to both Gram-negative and Gram-positive bacteria and acting through the classical complement pathway, can inhibit bacterial growth of Escherichia coli by lysis.Fil: Siroski, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); ArgentinaFil: Russi, N. B.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Formentini, Enrique Antonio. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaElsevier2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39587Siroski, Pablo Ariel; Russi, N. B.; Ortega, Hugo Hector; Formentini, Enrique Antonio; In vitro evaluation of synergistic activity between ciprofloxacin and broad snouted caiman serum against Escherichia coli; Elsevier; Research in Veterinary Science; 98; 2-2015; 98-1050034-5288CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0034528814003087info:eu-repo/semantics/altIdentifier/doi/10.1016/j.rvsc.2014.11.007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:36Zoai:ri.conicet.gov.ar:11336/39587instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:37.053CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro evaluation of synergistic activity between ciprofloxacin and broad snouted caiman serum against Escherichia coli
title In vitro evaluation of synergistic activity between ciprofloxacin and broad snouted caiman serum against Escherichia coli
spellingShingle In vitro evaluation of synergistic activity between ciprofloxacin and broad snouted caiman serum against Escherichia coli
Siroski, Pablo Ariel
Caiman Serum
Ciprofloxacin
Escherichia Coli
Synergism
Zootechnical Interest
title_short In vitro evaluation of synergistic activity between ciprofloxacin and broad snouted caiman serum against Escherichia coli
title_full In vitro evaluation of synergistic activity between ciprofloxacin and broad snouted caiman serum against Escherichia coli
title_fullStr In vitro evaluation of synergistic activity between ciprofloxacin and broad snouted caiman serum against Escherichia coli
title_full_unstemmed In vitro evaluation of synergistic activity between ciprofloxacin and broad snouted caiman serum against Escherichia coli
title_sort In vitro evaluation of synergistic activity between ciprofloxacin and broad snouted caiman serum against Escherichia coli
dc.creator.none.fl_str_mv Siroski, Pablo Ariel
Russi, N. B.
Ortega, Hugo Hector
Formentini, Enrique Antonio
author Siroski, Pablo Ariel
author_facet Siroski, Pablo Ariel
Russi, N. B.
Ortega, Hugo Hector
Formentini, Enrique Antonio
author_role author
author2 Russi, N. B.
Ortega, Hugo Hector
Formentini, Enrique Antonio
author2_role author
author
author
dc.subject.none.fl_str_mv Caiman Serum
Ciprofloxacin
Escherichia Coli
Synergism
Zootechnical Interest
topic Caiman Serum
Ciprofloxacin
Escherichia Coli
Synergism
Zootechnical Interest
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The in vitro synergistic activity between ciprofloxacin and serum of broad snouted caiman on Escherichia coli was studied. The estimated MIC value of ciprofloxacin was 0.0188 Μg/ml, and two assays of kill curve during 5 hours were performed: the first one in a standard culture medium and the second one in the presence of caiman serum. Different concentrations of ciprofloxacin were tested. Ciprofloxacin showed higher values of bacterial elimination rate in the presence of caiman serum in all concentrations tested. The combined activity of sub-inhibitory concentrations of ciprofloxacin and the humoral immune factors present in caiman serum determined an increase in the bacterial elimination observed in this assay. We suggest that the antibacterial activity of complement and natural antibodies present in caiman serum, which can bind to both Gram-negative and Gram-positive bacteria and acting through the classical complement pathway, can inhibit bacterial growth of Escherichia coli by lysis.
Fil: Siroski, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina
Fil: Russi, N. B.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Formentini, Enrique Antonio. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
description The in vitro synergistic activity between ciprofloxacin and serum of broad snouted caiman on Escherichia coli was studied. The estimated MIC value of ciprofloxacin was 0.0188 Μg/ml, and two assays of kill curve during 5 hours were performed: the first one in a standard culture medium and the second one in the presence of caiman serum. Different concentrations of ciprofloxacin were tested. Ciprofloxacin showed higher values of bacterial elimination rate in the presence of caiman serum in all concentrations tested. The combined activity of sub-inhibitory concentrations of ciprofloxacin and the humoral immune factors present in caiman serum determined an increase in the bacterial elimination observed in this assay. We suggest that the antibacterial activity of complement and natural antibodies present in caiman serum, which can bind to both Gram-negative and Gram-positive bacteria and acting through the classical complement pathway, can inhibit bacterial growth of Escherichia coli by lysis.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39587
Siroski, Pablo Ariel; Russi, N. B.; Ortega, Hugo Hector; Formentini, Enrique Antonio; In vitro evaluation of synergistic activity between ciprofloxacin and broad snouted caiman serum against Escherichia coli; Elsevier; Research in Veterinary Science; 98; 2-2015; 98-105
0034-5288
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39587
identifier_str_mv Siroski, Pablo Ariel; Russi, N. B.; Ortega, Hugo Hector; Formentini, Enrique Antonio; In vitro evaluation of synergistic activity between ciprofloxacin and broad snouted caiman serum against Escherichia coli; Elsevier; Research in Veterinary Science; 98; 2-2015; 98-105
0034-5288
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0034528814003087
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.rvsc.2014.11.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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