Prevention of in vitro oxidation of low density lipoproteins (LDL) by amaranth peptides released by gastrointestinal digestion
- Autores
- García Fillería, Susan Fiorella; Tironi, Valeria Anahi
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The objective of this work was to analyze the capacity of amaranth peptides generated by gastrointestinal digestion to prevent LDL oxidation. A simulated gastrointestinal digest from protein isolate (Id); Id fractions separated by gel filtration FPLC, and synthetic peptides (products of digestion) were evaluated. The evolution of conjugated dienes (CD) and, for most active samples, TBARS, fluorescent compounds (FC) evolution and electrophoretic mobility (EM) during Cu+2/H2O2-induced-LDL-oxidation were evaluated. Id was able to increase the lag time and to decrease the propagation rate for CD and FC formation; however, EM was not modified. Most active FPLC fractions (0.59–1.4 kDa) attained a complete inhibition of CD, and partial or total prevention of FC formation and electrophoretic changes. Peptides evaluation indicated that the presence of histidine, hydrophobic and aromatic residues would be important in the inhibition of Cu+2/H2O2-induced LDL oxidation. The most active were cationic or neutral peptides.
Fil: García Fillería, Susan Fiorella. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Tironi, Valeria Anahi. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina - Materia
-
Amaranth Proteins
Gastrointestinal Digestion
Ldl Oxidation
Peptides - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/57197
Ver los metadatos del registro completo
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Prevention of in vitro oxidation of low density lipoproteins (LDL) by amaranth peptides released by gastrointestinal digestionGarcía Fillería, Susan FiorellaTironi, Valeria AnahiAmaranth ProteinsGastrointestinal DigestionLdl OxidationPeptideshttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The objective of this work was to analyze the capacity of amaranth peptides generated by gastrointestinal digestion to prevent LDL oxidation. A simulated gastrointestinal digest from protein isolate (Id); Id fractions separated by gel filtration FPLC, and synthetic peptides (products of digestion) were evaluated. The evolution of conjugated dienes (CD) and, for most active samples, TBARS, fluorescent compounds (FC) evolution and electrophoretic mobility (EM) during Cu+2/H2O2-induced-LDL-oxidation were evaluated. Id was able to increase the lag time and to decrease the propagation rate for CD and FC formation; however, EM was not modified. Most active FPLC fractions (0.59–1.4 kDa) attained a complete inhibition of CD, and partial or total prevention of FC formation and electrophoretic changes. Peptides evaluation indicated that the presence of histidine, hydrophobic and aromatic residues would be important in the inhibition of Cu+2/H2O2-induced LDL oxidation. The most active were cationic or neutral peptides.Fil: García Fillería, Susan Fiorella. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Tironi, Valeria Anahi. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaElsevier2017-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/57197García Fillería, Susan Fiorella; Tironi, Valeria Anahi; Prevention of in vitro oxidation of low density lipoproteins (LDL) by amaranth peptides released by gastrointestinal digestion; Elsevier; Journal of Functional Foods; 34; 7-2017; 197-2061756-4646CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jff.2017.04.032info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1756464617302219info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:09Zoai:ri.conicet.gov.ar:11336/57197instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:09.859CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Prevention of in vitro oxidation of low density lipoproteins (LDL) by amaranth peptides released by gastrointestinal digestion |
title |
Prevention of in vitro oxidation of low density lipoproteins (LDL) by amaranth peptides released by gastrointestinal digestion |
spellingShingle |
Prevention of in vitro oxidation of low density lipoproteins (LDL) by amaranth peptides released by gastrointestinal digestion García Fillería, Susan Fiorella Amaranth Proteins Gastrointestinal Digestion Ldl Oxidation Peptides |
title_short |
Prevention of in vitro oxidation of low density lipoproteins (LDL) by amaranth peptides released by gastrointestinal digestion |
title_full |
Prevention of in vitro oxidation of low density lipoproteins (LDL) by amaranth peptides released by gastrointestinal digestion |
title_fullStr |
Prevention of in vitro oxidation of low density lipoproteins (LDL) by amaranth peptides released by gastrointestinal digestion |
title_full_unstemmed |
Prevention of in vitro oxidation of low density lipoproteins (LDL) by amaranth peptides released by gastrointestinal digestion |
title_sort |
Prevention of in vitro oxidation of low density lipoproteins (LDL) by amaranth peptides released by gastrointestinal digestion |
dc.creator.none.fl_str_mv |
García Fillería, Susan Fiorella Tironi, Valeria Anahi |
author |
García Fillería, Susan Fiorella |
author_facet |
García Fillería, Susan Fiorella Tironi, Valeria Anahi |
author_role |
author |
author2 |
Tironi, Valeria Anahi |
author2_role |
author |
dc.subject.none.fl_str_mv |
Amaranth Proteins Gastrointestinal Digestion Ldl Oxidation Peptides |
topic |
Amaranth Proteins Gastrointestinal Digestion Ldl Oxidation Peptides |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
The objective of this work was to analyze the capacity of amaranth peptides generated by gastrointestinal digestion to prevent LDL oxidation. A simulated gastrointestinal digest from protein isolate (Id); Id fractions separated by gel filtration FPLC, and synthetic peptides (products of digestion) were evaluated. The evolution of conjugated dienes (CD) and, for most active samples, TBARS, fluorescent compounds (FC) evolution and electrophoretic mobility (EM) during Cu+2/H2O2-induced-LDL-oxidation were evaluated. Id was able to increase the lag time and to decrease the propagation rate for CD and FC formation; however, EM was not modified. Most active FPLC fractions (0.59–1.4 kDa) attained a complete inhibition of CD, and partial or total prevention of FC formation and electrophoretic changes. Peptides evaluation indicated that the presence of histidine, hydrophobic and aromatic residues would be important in the inhibition of Cu+2/H2O2-induced LDL oxidation. The most active were cationic or neutral peptides. Fil: García Fillería, Susan Fiorella. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina Fil: Tironi, Valeria Anahi. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina |
description |
The objective of this work was to analyze the capacity of amaranth peptides generated by gastrointestinal digestion to prevent LDL oxidation. A simulated gastrointestinal digest from protein isolate (Id); Id fractions separated by gel filtration FPLC, and synthetic peptides (products of digestion) were evaluated. The evolution of conjugated dienes (CD) and, for most active samples, TBARS, fluorescent compounds (FC) evolution and electrophoretic mobility (EM) during Cu+2/H2O2-induced-LDL-oxidation were evaluated. Id was able to increase the lag time and to decrease the propagation rate for CD and FC formation; however, EM was not modified. Most active FPLC fractions (0.59–1.4 kDa) attained a complete inhibition of CD, and partial or total prevention of FC formation and electrophoretic changes. Peptides evaluation indicated that the presence of histidine, hydrophobic and aromatic residues would be important in the inhibition of Cu+2/H2O2-induced LDL oxidation. The most active were cationic or neutral peptides. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/57197 García Fillería, Susan Fiorella; Tironi, Valeria Anahi; Prevention of in vitro oxidation of low density lipoproteins (LDL) by amaranth peptides released by gastrointestinal digestion; Elsevier; Journal of Functional Foods; 34; 7-2017; 197-206 1756-4646 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/57197 |
identifier_str_mv |
García Fillería, Susan Fiorella; Tironi, Valeria Anahi; Prevention of in vitro oxidation of low density lipoproteins (LDL) by amaranth peptides released by gastrointestinal digestion; Elsevier; Journal of Functional Foods; 34; 7-2017; 197-206 1756-4646 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jff.2017.04.032 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1756464617302219 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613601037385728 |
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13.070432 |