Amaranth peptides from simulated gastrointestinal digestion: antioxidant activity against reactive species

Autores
Orsini Delgado, María Cecilia; Galleano, Monica Liliana; Añon, Maria Cristina; Tironi, Valeria Anahi
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We evaluated the capacity of simulated gastrointestinal digests or alcalase hydrolysates of protein isolates from amaranth to scavenge diverse physiologically relevant reactive species. The more active hydrolysate was obtained with the former method. Moreover, a prior alcalase treatment of the isolate followed by the same simulated gastrointestinal digestion did not improve the antioxidant capacity in any of the assays performed and even produced a negative effect under some conditions. Gastrointestinal digestion produced a strong increment in the scavenging capacity against peroxyl radicals (ORAC assay), hydroxyl radicals (ESR-OH assay), and peroxynitrites; thus decreasing the IC50 values to approximately 20, 25, and 20 %, respectively, of the levels attained with the nonhydrolyzed proteins. Metal chelation (HORAC assay) also enhanced respect to isolate levels, but to a lesser extent (decreasing IC50 values to only 50 %). The nitric-oxide– and superoxide-scavenging capacities of the digests were not relevant with respect to the methodologies used. The gastrointestinal digests from amaranth proteins acted against reactive species by different mechanisms, thus indicating the protein isolate to be a potential polyfunctional antioxidant ingredient.
Fil: Orsini Delgado, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Galleano, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires; Argentina
Fil: Añon, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Tironi, Valeria Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina
Materia
Amaranth Proteins And Peptides
Gastrointestinal Digestion
Antioxidant Activity
Oxygen And Nitrogen Reactive Species
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10101
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10101
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Amaranth peptides from simulated gastrointestinal digestion: antioxidant activity against reactive speciesOrsini Delgado, María CeciliaGalleano, Monica LilianaAñon, Maria CristinaTironi, Valeria AnahiAmaranth Proteins And PeptidesGastrointestinal DigestionAntioxidant ActivityOxygen And Nitrogen Reactive Specieshttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2We evaluated the capacity of simulated gastrointestinal digests or alcalase hydrolysates of protein isolates from amaranth to scavenge diverse physiologically relevant reactive species. The more active hydrolysate was obtained with the former method. Moreover, a prior alcalase treatment of the isolate followed by the same simulated gastrointestinal digestion did not improve the antioxidant capacity in any of the assays performed and even produced a negative effect under some conditions. Gastrointestinal digestion produced a strong increment in the scavenging capacity against peroxyl radicals (ORAC assay), hydroxyl radicals (ESR-OH assay), and peroxynitrites; thus decreasing the IC50 values to approximately 20, 25, and 20 %, respectively, of the levels attained with the nonhydrolyzed proteins. Metal chelation (HORAC assay) also enhanced respect to isolate levels, but to a lesser extent (decreasing IC50 values to only 50 %). The nitric-oxide– and superoxide-scavenging capacities of the digests were not relevant with respect to the methodologies used. The gastrointestinal digests from amaranth proteins acted against reactive species by different mechanisms, thus indicating the protein isolate to be a potential polyfunctional antioxidant ingredient.Fil: Orsini Delgado, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; ArgentinaFil: Galleano, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires; ArgentinaFil: Añon, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; ArgentinaFil: Tironi, Valeria Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; ArgentinaSpringer2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10101Orsini Delgado, María Cecilia; Galleano, Monica Liliana; Añon, Maria Cristina; Tironi, Valeria Anahi; Amaranth peptides from simulated gastrointestinal digestion: antioxidant activity against reactive species; Springer; Plant Foods For Human Nutrition; 70; 1; 3-2015; 27-340921-96681573-9104enginfo:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1007/s11130-014-0457-2info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs11130-014-0457-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:09Zoai:ri.conicet.gov.ar:11336/10101instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:10.198CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Amaranth peptides from simulated gastrointestinal digestion: antioxidant activity against reactive species
title Amaranth peptides from simulated gastrointestinal digestion: antioxidant activity against reactive species
spellingShingle Amaranth peptides from simulated gastrointestinal digestion: antioxidant activity against reactive species
Orsini Delgado, María Cecilia
Amaranth Proteins And Peptides
Gastrointestinal Digestion
Antioxidant Activity
Oxygen And Nitrogen Reactive Species
title_short Amaranth peptides from simulated gastrointestinal digestion: antioxidant activity against reactive species
title_full Amaranth peptides from simulated gastrointestinal digestion: antioxidant activity against reactive species
title_fullStr Amaranth peptides from simulated gastrointestinal digestion: antioxidant activity against reactive species
title_full_unstemmed Amaranth peptides from simulated gastrointestinal digestion: antioxidant activity against reactive species
title_sort Amaranth peptides from simulated gastrointestinal digestion: antioxidant activity against reactive species
dc.creator.none.fl_str_mv Orsini Delgado, María Cecilia
Galleano, Monica Liliana
Añon, Maria Cristina
Tironi, Valeria Anahi
author Orsini Delgado, María Cecilia
author_facet Orsini Delgado, María Cecilia
Galleano, Monica Liliana
Añon, Maria Cristina
Tironi, Valeria Anahi
author_role author
author2 Galleano, Monica Liliana
Añon, Maria Cristina
Tironi, Valeria Anahi
author2_role author
author
author
dc.subject.none.fl_str_mv Amaranth Proteins And Peptides
Gastrointestinal Digestion
Antioxidant Activity
Oxygen And Nitrogen Reactive Species
topic Amaranth Proteins And Peptides
Gastrointestinal Digestion
Antioxidant Activity
Oxygen And Nitrogen Reactive Species
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv We evaluated the capacity of simulated gastrointestinal digests or alcalase hydrolysates of protein isolates from amaranth to scavenge diverse physiologically relevant reactive species. The more active hydrolysate was obtained with the former method. Moreover, a prior alcalase treatment of the isolate followed by the same simulated gastrointestinal digestion did not improve the antioxidant capacity in any of the assays performed and even produced a negative effect under some conditions. Gastrointestinal digestion produced a strong increment in the scavenging capacity against peroxyl radicals (ORAC assay), hydroxyl radicals (ESR-OH assay), and peroxynitrites; thus decreasing the IC50 values to approximately 20, 25, and 20 %, respectively, of the levels attained with the nonhydrolyzed proteins. Metal chelation (HORAC assay) also enhanced respect to isolate levels, but to a lesser extent (decreasing IC50 values to only 50 %). The nitric-oxide– and superoxide-scavenging capacities of the digests were not relevant with respect to the methodologies used. The gastrointestinal digests from amaranth proteins acted against reactive species by different mechanisms, thus indicating the protein isolate to be a potential polyfunctional antioxidant ingredient.
Fil: Orsini Delgado, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Galleano, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular; Argentina. Universidad de Buenos Aires; Argentina
Fil: Añon, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Tironi, Valeria Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigaciones en Criotecnología de Alimentos (i); Argentina. Universidad Nacional de La Plata; Argentina
description We evaluated the capacity of simulated gastrointestinal digests or alcalase hydrolysates of protein isolates from amaranth to scavenge diverse physiologically relevant reactive species. The more active hydrolysate was obtained with the former method. Moreover, a prior alcalase treatment of the isolate followed by the same simulated gastrointestinal digestion did not improve the antioxidant capacity in any of the assays performed and even produced a negative effect under some conditions. Gastrointestinal digestion produced a strong increment in the scavenging capacity against peroxyl radicals (ORAC assay), hydroxyl radicals (ESR-OH assay), and peroxynitrites; thus decreasing the IC50 values to approximately 20, 25, and 20 %, respectively, of the levels attained with the nonhydrolyzed proteins. Metal chelation (HORAC assay) also enhanced respect to isolate levels, but to a lesser extent (decreasing IC50 values to only 50 %). The nitric-oxide– and superoxide-scavenging capacities of the digests were not relevant with respect to the methodologies used. The gastrointestinal digests from amaranth proteins acted against reactive species by different mechanisms, thus indicating the protein isolate to be a potential polyfunctional antioxidant ingredient.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10101
Orsini Delgado, María Cecilia; Galleano, Monica Liliana; Añon, Maria Cristina; Tironi, Valeria Anahi; Amaranth peptides from simulated gastrointestinal digestion: antioxidant activity against reactive species; Springer; Plant Foods For Human Nutrition; 70; 1; 3-2015; 27-34
0921-9668
1573-9104
url http://hdl.handle.net/11336/10101
identifier_str_mv Orsini Delgado, María Cecilia; Galleano, Monica Liliana; Añon, Maria Cristina; Tironi, Valeria Anahi; Amaranth peptides from simulated gastrointestinal digestion: antioxidant activity against reactive species; Springer; Plant Foods For Human Nutrition; 70; 1; 3-2015; 27-34
0921-9668
1573-9104
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1007/s11130-014-0457-2
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs11130-014-0457-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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