Is apomictic seed development influenced/controlled by epigenetic factors?

Autores
Marconi, G.; Soliman, Mariano; Zappacosta, Diego Carlos; Di Marsico, M.; Bocchini, M.; Gallardo, Jimena Alicia; Delgado, L.; Echenique, Carmen Viviana; Albertini, E.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Several lines of evidence suggest that transitions during reproduction and early seeddevelopment are epigenetically regulated by dynamic changes in chromatin state. Moreover,deregulation of key developmental steps in sexual processes are thought to causeapomixis, and supporters of this hypothesis justify it based on the coexistence of sex andapomixis in the same individual. For example, some authors found that DNA methylationderegulation in reproductive cells induce apomeiosis-like phenotypes suggesting thatspecialization of a DNA methylation pathway acts upon germline or germline associatedcells. With this aim in mind, we decided to investigate DNA methylation differencesbetween apomictic and sexual genotypes of two species, Paspalum rufum and Eragrostis curvula. High-throughput DNA sequencing technologies have enabled the measurementof cytosine methylation on a genome-wide scale. Many technologies have been developedover the past decade to measure DNA methylation. MCSeEd (Methylation ContentSensitive Enzyme ddRAD) is a reduced-representation, reference-free, cost-effective approachfor characterizing whole genome methylation patterns across different methylationcontexts (CG, CHG, CHH, 6 mA) that we have recently developed at the University ofPerugia. DNA from triplicate panicle samples of contrasting reproductive mode materialswere digested with enzymes sensitive to DNA methylation (AciI, PstI, EcoT22I, and DpnII,for the CG, CHG, CHH and 6 mA contexts, respectively), and libraries were generated andsequenced in Illumina platform. Several differentially methylated genomic regions werefound and associated to annotated genes that were classified with the BLAST2GO software.Several genes already linked to apomixis (i.e., SERK, APOSTART) were found to be bothdifferentially methylated and differentially expressed.
Fil: Marconi, G.. Università di Perugia; Italia
Fil: Soliman, Mariano. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Di Marsico, M.. Università di Perugia; Italia
Fil: Bocchini, M.. Università di Perugia; Italia
Fil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Delgado, L.. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Albertini, E.. Università di Perugia; Italia
7th biennial Seminars on Advances in Apomixis Research
Bahía Blanca
Argentina
Centro de Recursos Naturales Renovables de la Zona Semiarida
Materia
EPIGENETICA
METILACION DEL ADN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195365
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/195365
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network_name_str CONICET Digital (CONICET)
spelling Is apomictic seed development influenced/controlled by epigenetic factors?Marconi, G.Soliman, MarianoZappacosta, Diego CarlosDi Marsico, M.Bocchini, M.Gallardo, Jimena AliciaDelgado, L.Echenique, Carmen VivianaAlbertini, E.EPIGENETICAMETILACION DEL ADNhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Several lines of evidence suggest that transitions during reproduction and early seeddevelopment are epigenetically regulated by dynamic changes in chromatin state. Moreover,deregulation of key developmental steps in sexual processes are thought to causeapomixis, and supporters of this hypothesis justify it based on the coexistence of sex andapomixis in the same individual. For example, some authors found that DNA methylationderegulation in reproductive cells induce apomeiosis-like phenotypes suggesting thatspecialization of a DNA methylation pathway acts upon germline or germline associatedcells. With this aim in mind, we decided to investigate DNA methylation differencesbetween apomictic and sexual genotypes of two species, Paspalum rufum and Eragrostis curvula. High-throughput DNA sequencing technologies have enabled the measurementof cytosine methylation on a genome-wide scale. Many technologies have been developedover the past decade to measure DNA methylation. MCSeEd (Methylation ContentSensitive Enzyme ddRAD) is a reduced-representation, reference-free, cost-effective approachfor characterizing whole genome methylation patterns across different methylationcontexts (CG, CHG, CHH, 6 mA) that we have recently developed at the University ofPerugia. DNA from triplicate panicle samples of contrasting reproductive mode materialswere digested with enzymes sensitive to DNA methylation (AciI, PstI, EcoT22I, and DpnII,for the CG, CHG, CHH and 6 mA contexts, respectively), and libraries were generated andsequenced in Illumina platform. Several differentially methylated genomic regions werefound and associated to annotated genes that were classified with the BLAST2GO software.Several genes already linked to apomixis (i.e., SERK, APOSTART) were found to be bothdifferentially methylated and differentially expressed.Fil: Marconi, G.. Università di Perugia; ItaliaFil: Soliman, Mariano. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Di Marsico, M.. Università di Perugia; ItaliaFil: Bocchini, M.. Università di Perugia; ItaliaFil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Delgado, L.. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Albertini, E.. Università di Perugia; Italia7th biennial Seminars on Advances in Apomixis ResearchBahía BlancaArgentinaCentro de Recursos Naturales Renovables de la Zona SemiaridaMultidisciplinary Digital Publishing Institute2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectWorkshopJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195365Is apomictic seed development influenced/controlled by epigenetic factors?; 7th biennial Seminars on Advances in Apomixis Research; Bahía Blanca; Argentina; 2020; 12-122223-7747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/3/565info:eu-repo/semantics/altIdentifier/doi/10.3390/plants10030565Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:37Zoai:ri.conicet.gov.ar:11336/195365instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:37.905CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Is apomictic seed development influenced/controlled by epigenetic factors?
title Is apomictic seed development influenced/controlled by epigenetic factors?
spellingShingle Is apomictic seed development influenced/controlled by epigenetic factors?
Marconi, G.
EPIGENETICA
METILACION DEL ADN
title_short Is apomictic seed development influenced/controlled by epigenetic factors?
title_full Is apomictic seed development influenced/controlled by epigenetic factors?
title_fullStr Is apomictic seed development influenced/controlled by epigenetic factors?
title_full_unstemmed Is apomictic seed development influenced/controlled by epigenetic factors?
title_sort Is apomictic seed development influenced/controlled by epigenetic factors?
dc.creator.none.fl_str_mv Marconi, G.
Soliman, Mariano
Zappacosta, Diego Carlos
Di Marsico, M.
Bocchini, M.
Gallardo, Jimena Alicia
Delgado, L.
Echenique, Carmen Viviana
Albertini, E.
author Marconi, G.
author_facet Marconi, G.
Soliman, Mariano
Zappacosta, Diego Carlos
Di Marsico, M.
Bocchini, M.
Gallardo, Jimena Alicia
Delgado, L.
Echenique, Carmen Viviana
Albertini, E.
author_role author
author2 Soliman, Mariano
Zappacosta, Diego Carlos
Di Marsico, M.
Bocchini, M.
Gallardo, Jimena Alicia
Delgado, L.
Echenique, Carmen Viviana
Albertini, E.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv EPIGENETICA
METILACION DEL ADN
topic EPIGENETICA
METILACION DEL ADN
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Several lines of evidence suggest that transitions during reproduction and early seeddevelopment are epigenetically regulated by dynamic changes in chromatin state. Moreover,deregulation of key developmental steps in sexual processes are thought to causeapomixis, and supporters of this hypothesis justify it based on the coexistence of sex andapomixis in the same individual. For example, some authors found that DNA methylationderegulation in reproductive cells induce apomeiosis-like phenotypes suggesting thatspecialization of a DNA methylation pathway acts upon germline or germline associatedcells. With this aim in mind, we decided to investigate DNA methylation differencesbetween apomictic and sexual genotypes of two species, Paspalum rufum and Eragrostis curvula. High-throughput DNA sequencing technologies have enabled the measurementof cytosine methylation on a genome-wide scale. Many technologies have been developedover the past decade to measure DNA methylation. MCSeEd (Methylation ContentSensitive Enzyme ddRAD) is a reduced-representation, reference-free, cost-effective approachfor characterizing whole genome methylation patterns across different methylationcontexts (CG, CHG, CHH, 6 mA) that we have recently developed at the University ofPerugia. DNA from triplicate panicle samples of contrasting reproductive mode materialswere digested with enzymes sensitive to DNA methylation (AciI, PstI, EcoT22I, and DpnII,for the CG, CHG, CHH and 6 mA contexts, respectively), and libraries were generated andsequenced in Illumina platform. Several differentially methylated genomic regions werefound and associated to annotated genes that were classified with the BLAST2GO software.Several genes already linked to apomixis (i.e., SERK, APOSTART) were found to be bothdifferentially methylated and differentially expressed.
Fil: Marconi, G.. Università di Perugia; Italia
Fil: Soliman, Mariano. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Di Marsico, M.. Università di Perugia; Italia
Fil: Bocchini, M.. Università di Perugia; Italia
Fil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Delgado, L.. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Albertini, E.. Università di Perugia; Italia
7th biennial Seminars on Advances in Apomixis Research
Bahía Blanca
Argentina
Centro de Recursos Naturales Renovables de la Zona Semiarida
description Several lines of evidence suggest that transitions during reproduction and early seeddevelopment are epigenetically regulated by dynamic changes in chromatin state. Moreover,deregulation of key developmental steps in sexual processes are thought to causeapomixis, and supporters of this hypothesis justify it based on the coexistence of sex andapomixis in the same individual. For example, some authors found that DNA methylationderegulation in reproductive cells induce apomeiosis-like phenotypes suggesting thatspecialization of a DNA methylation pathway acts upon germline or germline associatedcells. With this aim in mind, we decided to investigate DNA methylation differencesbetween apomictic and sexual genotypes of two species, Paspalum rufum and Eragrostis curvula. High-throughput DNA sequencing technologies have enabled the measurementof cytosine methylation on a genome-wide scale. Many technologies have been developedover the past decade to measure DNA methylation. MCSeEd (Methylation ContentSensitive Enzyme ddRAD) is a reduced-representation, reference-free, cost-effective approachfor characterizing whole genome methylation patterns across different methylationcontexts (CG, CHG, CHH, 6 mA) that we have recently developed at the University ofPerugia. DNA from triplicate panicle samples of contrasting reproductive mode materialswere digested with enzymes sensitive to DNA methylation (AciI, PstI, EcoT22I, and DpnII,for the CG, CHG, CHH and 6 mA contexts, respectively), and libraries were generated andsequenced in Illumina platform. Several differentially methylated genomic regions werefound and associated to annotated genes that were classified with the BLAST2GO software.Several genes already linked to apomixis (i.e., SERK, APOSTART) were found to be bothdifferentially methylated and differentially expressed.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Workshop
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195365
Is apomictic seed development influenced/controlled by epigenetic factors?; 7th biennial Seminars on Advances in Apomixis Research; Bahía Blanca; Argentina; 2020; 12-12
2223-7747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195365
identifier_str_mv Is apomictic seed development influenced/controlled by epigenetic factors?; 7th biennial Seminars on Advances in Apomixis Research; Bahía Blanca; Argentina; 2020; 12-12
2223-7747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/3/565
info:eu-repo/semantics/altIdentifier/doi/10.3390/plants10030565
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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