General biochemical and immunological characterization of the venom from the scorpion Tityus trivittatus of Argentina

Autores
de Roodt, Adolfo Rafael; Coronas, Fredy I.V.; Lago, Néstor Rubén; González, María E.; Laskowicz, Rodrigo D.; Beltramino, Juan C.; Saavedra, Silvina; López, Raúl A.; Reati, Gustavo J.; Vucharchuk, Miriam G.; Bazán, Eduardo; Varni, Liliana; Salomón, Oscar Daniel; Possani, Lourival D.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tityus trivittatus is the Argentinean scorpion reported to cause the majority of human fatalities in the country, however no systematic studies have been conducted with the venom of this species. This communication describes a general biochemical and immunological characterization of the venom obtained from T. trivittatus scorpions collected in the city of Buenos Aires and various provinces of Argentina: Catamarca, Cordoba, Entre Rios, La Rioja, Santa Fe and Santiago del Estero. These are places where human accidents were reported to occur due to this scorpion. For comparative purposes two types of samples were assayed: whole soluble venom obtained by electrical stimulation and supernatant from homogenized venomous glands. Two strains of mice (NIH and CF-1) were used for LD50 determinations by two distinct routes of administration (intravenously and intraperitoneally). Important variations were found that goes from 0.5 to 12 mg/kg mouse body weight. Samples of soluble venom were always more potent than Telson homogenates. More complex pattern was observed in homogenates compared to soluble venom, as expected. This was supported by gel electrophoretic analysis and high performance liquid chromatographic (HPLC) separations. Additionally, the HPLC profile was enriched in proteins resolved at similar elution times as other known toxins from scorpion venoms studied. Immune enzymatic assays were also conducted comparatively, using four different anti-venoms commercially available for treatment of scorpion stings (Argentinean antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn from the Mexican Bioclon Institute). Cross-reactivities were observed and are reported among the various venoms and anti-venoms used. Lung, heart, liver and pancreas pathological modifications were observed on tissues of intoxicated mice. It seems that there are important variations on the venom compositions of the various samples studied and reported here, depending on the geographical area where the scorpions were captured. The results reported here are important for the clinical outcome of human accidents.
Fil: de Roodt, Adolfo Rafael. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Coronas, Fredy I.V.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Lago, Néstor Rubén. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología; Argentina
Fil: González, María E.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Laskowicz, Rodrigo D.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Beltramino, Juan C.. Provincia de Santa Fe. Ministerio de Salud. Hospital de Niños “Orlando Alassia”; Argentina
Fil: Saavedra, Silvina. Provincia de Entre Ríos. Ministerio de Salud. Dirección de Zoonosis; Argentina
Fil: López, Raúl A.. Provincia de Catamarca. Ministerio de Salud. Departamento de Zoonosis; Argentina
Fil: Reati, Gustavo J.. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Zoología Aplicada; Argentina
Fil: Vucharchuk, Miriam G.. Provincia de Santiago del Estero. Ministerio de Salud. Instituto de Animales Venenosos “Jorge W. Abalos"; Argentina
Fil: Bazán, Eduardo. Provincia de La Rioja. Ministerio de Salud. Dirección de Epidemiología; Argentina
Fil: Varni, Liliana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Salomón, Oscar Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Possani, Lourival D.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Materia
Chromatographic Separation
Ld50
Scorpion
Tityus Trivittatus
Toxicity
Venom
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79349
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79349
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network_name_str CONICET Digital (CONICET)
spelling General biochemical and immunological characterization of the venom from the scorpion Tityus trivittatus of Argentinade Roodt, Adolfo RafaelCoronas, Fredy I.V.Lago, Néstor RubénGonzález, María E.Laskowicz, Rodrigo D.Beltramino, Juan C.Saavedra, SilvinaLópez, Raúl A.Reati, Gustavo J.Vucharchuk, Miriam G.Bazán, EduardoVarni, LilianaSalomón, Oscar DanielPossani, Lourival D.Chromatographic SeparationLd50ScorpionTityus TrivittatusToxicityVenomhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Tityus trivittatus is the Argentinean scorpion reported to cause the majority of human fatalities in the country, however no systematic studies have been conducted with the venom of this species. This communication describes a general biochemical and immunological characterization of the venom obtained from T. trivittatus scorpions collected in the city of Buenos Aires and various provinces of Argentina: Catamarca, Cordoba, Entre Rios, La Rioja, Santa Fe and Santiago del Estero. These are places where human accidents were reported to occur due to this scorpion. For comparative purposes two types of samples were assayed: whole soluble venom obtained by electrical stimulation and supernatant from homogenized venomous glands. Two strains of mice (NIH and CF-1) were used for LD50 determinations by two distinct routes of administration (intravenously and intraperitoneally). Important variations were found that goes from 0.5 to 12 mg/kg mouse body weight. Samples of soluble venom were always more potent than Telson homogenates. More complex pattern was observed in homogenates compared to soluble venom, as expected. This was supported by gel electrophoretic analysis and high performance liquid chromatographic (HPLC) separations. Additionally, the HPLC profile was enriched in proteins resolved at similar elution times as other known toxins from scorpion venoms studied. Immune enzymatic assays were also conducted comparatively, using four different anti-venoms commercially available for treatment of scorpion stings (Argentinean antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn from the Mexican Bioclon Institute). Cross-reactivities were observed and are reported among the various venoms and anti-venoms used. Lung, heart, liver and pancreas pathological modifications were observed on tissues of intoxicated mice. It seems that there are important variations on the venom compositions of the various samples studied and reported here, depending on the geographical area where the scorpions were captured. The results reported here are important for the clinical outcome of human accidents.Fil: de Roodt, Adolfo Rafael. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Coronas, Fredy I.V.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Lago, Néstor Rubén. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología; ArgentinaFil: González, María E.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Laskowicz, Rodrigo D.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Beltramino, Juan C.. Provincia de Santa Fe. Ministerio de Salud. Hospital de Niños “Orlando Alassia”; ArgentinaFil: Saavedra, Silvina. Provincia de Entre Ríos. Ministerio de Salud. Dirección de Zoonosis; ArgentinaFil: López, Raúl A.. Provincia de Catamarca. Ministerio de Salud. Departamento de Zoonosis; ArgentinaFil: Reati, Gustavo J.. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Zoología Aplicada; ArgentinaFil: Vucharchuk, Miriam G.. Provincia de Santiago del Estero. Ministerio de Salud. Instituto de Animales Venenosos “Jorge W. Abalos"; ArgentinaFil: Bazán, Eduardo. Provincia de La Rioja. Ministerio de Salud. Dirección de Epidemiología; ArgentinaFil: Varni, Liliana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Salomón, Oscar Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Possani, Lourival D.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoPergamon-Elsevier Science Ltd2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79349de Roodt, Adolfo Rafael; Coronas, Fredy I.V.; Lago, Néstor Rubén; González, María E.; Laskowicz, Rodrigo D.; et al.; General biochemical and immunological characterization of the venom from the scorpion Tityus trivittatus of Argentina; Pergamon-Elsevier Science Ltd; Toxicon; 55; 2-3; 2-2010; 307-3190041-01011879-3150CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2009.08.014info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0041010109004231info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:33Zoai:ri.conicet.gov.ar:11336/79349instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:33.472CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv General biochemical and immunological characterization of the venom from the scorpion Tityus trivittatus of Argentina
title General biochemical and immunological characterization of the venom from the scorpion Tityus trivittatus of Argentina
spellingShingle General biochemical and immunological characterization of the venom from the scorpion Tityus trivittatus of Argentina
de Roodt, Adolfo Rafael
Chromatographic Separation
Ld50
Scorpion
Tityus Trivittatus
Toxicity
Venom
title_short General biochemical and immunological characterization of the venom from the scorpion Tityus trivittatus of Argentina
title_full General biochemical and immunological characterization of the venom from the scorpion Tityus trivittatus of Argentina
title_fullStr General biochemical and immunological characterization of the venom from the scorpion Tityus trivittatus of Argentina
title_full_unstemmed General biochemical and immunological characterization of the venom from the scorpion Tityus trivittatus of Argentina
title_sort General biochemical and immunological characterization of the venom from the scorpion Tityus trivittatus of Argentina
dc.creator.none.fl_str_mv de Roodt, Adolfo Rafael
Coronas, Fredy I.V.
Lago, Néstor Rubén
González, María E.
Laskowicz, Rodrigo D.
Beltramino, Juan C.
Saavedra, Silvina
López, Raúl A.
Reati, Gustavo J.
Vucharchuk, Miriam G.
Bazán, Eduardo
Varni, Liliana
Salomón, Oscar Daniel
Possani, Lourival D.
author de Roodt, Adolfo Rafael
author_facet de Roodt, Adolfo Rafael
Coronas, Fredy I.V.
Lago, Néstor Rubén
González, María E.
Laskowicz, Rodrigo D.
Beltramino, Juan C.
Saavedra, Silvina
López, Raúl A.
Reati, Gustavo J.
Vucharchuk, Miriam G.
Bazán, Eduardo
Varni, Liliana
Salomón, Oscar Daniel
Possani, Lourival D.
author_role author
author2 Coronas, Fredy I.V.
Lago, Néstor Rubén
González, María E.
Laskowicz, Rodrigo D.
Beltramino, Juan C.
Saavedra, Silvina
López, Raúl A.
Reati, Gustavo J.
Vucharchuk, Miriam G.
Bazán, Eduardo
Varni, Liliana
Salomón, Oscar Daniel
Possani, Lourival D.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chromatographic Separation
Ld50
Scorpion
Tityus Trivittatus
Toxicity
Venom
topic Chromatographic Separation
Ld50
Scorpion
Tityus Trivittatus
Toxicity
Venom
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Tityus trivittatus is the Argentinean scorpion reported to cause the majority of human fatalities in the country, however no systematic studies have been conducted with the venom of this species. This communication describes a general biochemical and immunological characterization of the venom obtained from T. trivittatus scorpions collected in the city of Buenos Aires and various provinces of Argentina: Catamarca, Cordoba, Entre Rios, La Rioja, Santa Fe and Santiago del Estero. These are places where human accidents were reported to occur due to this scorpion. For comparative purposes two types of samples were assayed: whole soluble venom obtained by electrical stimulation and supernatant from homogenized venomous glands. Two strains of mice (NIH and CF-1) were used for LD50 determinations by two distinct routes of administration (intravenously and intraperitoneally). Important variations were found that goes from 0.5 to 12 mg/kg mouse body weight. Samples of soluble venom were always more potent than Telson homogenates. More complex pattern was observed in homogenates compared to soluble venom, as expected. This was supported by gel electrophoretic analysis and high performance liquid chromatographic (HPLC) separations. Additionally, the HPLC profile was enriched in proteins resolved at similar elution times as other known toxins from scorpion venoms studied. Immune enzymatic assays were also conducted comparatively, using four different anti-venoms commercially available for treatment of scorpion stings (Argentinean antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn from the Mexican Bioclon Institute). Cross-reactivities were observed and are reported among the various venoms and anti-venoms used. Lung, heart, liver and pancreas pathological modifications were observed on tissues of intoxicated mice. It seems that there are important variations on the venom compositions of the various samples studied and reported here, depending on the geographical area where the scorpions were captured. The results reported here are important for the clinical outcome of human accidents.
Fil: de Roodt, Adolfo Rafael. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Coronas, Fredy I.V.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Lago, Néstor Rubén. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología; Argentina
Fil: González, María E.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Laskowicz, Rodrigo D.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Beltramino, Juan C.. Provincia de Santa Fe. Ministerio de Salud. Hospital de Niños “Orlando Alassia”; Argentina
Fil: Saavedra, Silvina. Provincia de Entre Ríos. Ministerio de Salud. Dirección de Zoonosis; Argentina
Fil: López, Raúl A.. Provincia de Catamarca. Ministerio de Salud. Departamento de Zoonosis; Argentina
Fil: Reati, Gustavo J.. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Zoología Aplicada; Argentina
Fil: Vucharchuk, Miriam G.. Provincia de Santiago del Estero. Ministerio de Salud. Instituto de Animales Venenosos “Jorge W. Abalos"; Argentina
Fil: Bazán, Eduardo. Provincia de La Rioja. Ministerio de Salud. Dirección de Epidemiología; Argentina
Fil: Varni, Liliana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Salomón, Oscar Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Possani, Lourival D.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
description Tityus trivittatus is the Argentinean scorpion reported to cause the majority of human fatalities in the country, however no systematic studies have been conducted with the venom of this species. This communication describes a general biochemical and immunological characterization of the venom obtained from T. trivittatus scorpions collected in the city of Buenos Aires and various provinces of Argentina: Catamarca, Cordoba, Entre Rios, La Rioja, Santa Fe and Santiago del Estero. These are places where human accidents were reported to occur due to this scorpion. For comparative purposes two types of samples were assayed: whole soluble venom obtained by electrical stimulation and supernatant from homogenized venomous glands. Two strains of mice (NIH and CF-1) were used for LD50 determinations by two distinct routes of administration (intravenously and intraperitoneally). Important variations were found that goes from 0.5 to 12 mg/kg mouse body weight. Samples of soluble venom were always more potent than Telson homogenates. More complex pattern was observed in homogenates compared to soluble venom, as expected. This was supported by gel electrophoretic analysis and high performance liquid chromatographic (HPLC) separations. Additionally, the HPLC profile was enriched in proteins resolved at similar elution times as other known toxins from scorpion venoms studied. Immune enzymatic assays were also conducted comparatively, using four different anti-venoms commercially available for treatment of scorpion stings (Argentinean antidote from INPB, two anti-venoms from Butantan Institute of Brazil and Alacramyn from the Mexican Bioclon Institute). Cross-reactivities were observed and are reported among the various venoms and anti-venoms used. Lung, heart, liver and pancreas pathological modifications were observed on tissues of intoxicated mice. It seems that there are important variations on the venom compositions of the various samples studied and reported here, depending on the geographical area where the scorpions were captured. The results reported here are important for the clinical outcome of human accidents.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79349
de Roodt, Adolfo Rafael; Coronas, Fredy I.V.; Lago, Néstor Rubén; González, María E.; Laskowicz, Rodrigo D.; et al.; General biochemical and immunological characterization of the venom from the scorpion Tityus trivittatus of Argentina; Pergamon-Elsevier Science Ltd; Toxicon; 55; 2-3; 2-2010; 307-319
0041-0101
1879-3150
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79349
identifier_str_mv de Roodt, Adolfo Rafael; Coronas, Fredy I.V.; Lago, Néstor Rubén; González, María E.; Laskowicz, Rodrigo D.; et al.; General biochemical and immunological characterization of the venom from the scorpion Tityus trivittatus of Argentina; Pergamon-Elsevier Science Ltd; Toxicon; 55; 2-3; 2-2010; 307-319
0041-0101
1879-3150
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2009.08.014
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0041010109004231
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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