Embryogenic cell suspensions from different explants and cultivars of Eragrostis curvula (Schrad.) Nees

Autores
Echenique, Carmen Viviana; Díaz, Marina; Polci, Pablo Aldo; Mroginski, Luis Amado
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was the establishment of embryogenic calli and cell suspensions from different explants and cultivars ofweeping lovegrass, Eragrostis curvula (Schrad.) Nees, to be used as targets for biolistic transformation. Calli were initiated from immature inflorescences, seeds, embryos, leafbases and root tips. Modified MS medium (Murashige and Skoog, 1962) was used for calli induction and proliferation. Cell suspensions were established and maintained in AAF medium (Wang et al., 1993). Morphogenic calli, embryogenic cell suspensions of moderate growth rate - consisting mainly of compact proembryogenic cell c1usters- and green plants were obtained from all the explants and cultivars assayed, except root tips. Both, explant and genotype were very important factors to be considered in order to obtain a morphogenic response and to establish cell suspensions from this grass. The statistical analysis detected interaction between both factors, explants and genotypes. Immature inflorescences were the best source of explant and Kromdraai was the cultivar that showed the best morphogenic response (expressed as the percentage of calli/explant and the percentage of calli with green spots -every green spot developed into green plants-) with inflorescences, embryos and leafbases. For Morpa and Don Pablo embryos as explants were less responsive than seeds and leafbases. There were no differences in leafbases for all the three cultivars analysed.
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Díaz, Marina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Polci, Pablo Aldo. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Materia
PLANT TISSUE CULTURE
CELL SUSPENSIONS
GRAMINEAE
PLANT REGENERATION
Eragrostis curvula
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/45111
Acceder
id CONICETDig_4a52d13a1f46c4de4d7fd4d2876020ac
oai_identifier_str oai:ri.conicet.gov.ar:11336/45111
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Embryogenic cell suspensions from different explants and cultivars of Eragrostis curvula (Schrad.) NeesEchenique, Carmen VivianaDíaz, MarinaPolci, Pablo AldoMroginski, Luis AmadoPLANT TISSUE CULTURECELL SUSPENSIONSGRAMINEAEPLANT REGENERATIONEragrostis curvulahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this work was the establishment of embryogenic calli and cell suspensions from different explants and cultivars ofweeping lovegrass, Eragrostis curvula (Schrad.) Nees, to be used as targets for biolistic transformation. Calli were initiated from immature inflorescences, seeds, embryos, leafbases and root tips. Modified MS medium (Murashige and Skoog, 1962) was used for calli induction and proliferation. Cell suspensions were established and maintained in AAF medium (Wang et al., 1993). Morphogenic calli, embryogenic cell suspensions of moderate growth rate - consisting mainly of compact proembryogenic cell c1usters- and green plants were obtained from all the explants and cultivars assayed, except root tips. Both, explant and genotype were very important factors to be considered in order to obtain a morphogenic response and to establish cell suspensions from this grass. The statistical analysis detected interaction between both factors, explants and genotypes. Immature inflorescences were the best source of explant and Kromdraai was the cultivar that showed the best morphogenic response (expressed as the percentage of calli/explant and the percentage of calli with green spots -every green spot developed into green plants-) with inflorescences, embryos and leafbases. For Morpa and Don Pablo embryos as explants were less responsive than seeds and leafbases. There were no differences in leafbases for all the three cultivars analysed.Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Díaz, Marina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Polci, Pablo Aldo. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaInstituto de Histología y Embriología “Dr. Mario H. Burgos”2001-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/45111Echenique, Carmen Viviana; Díaz, Marina; Polci, Pablo Aldo; Mroginski, Luis Amado; Embryogenic cell suspensions from different explants and cultivars of Eragrostis curvula (Schrad.) Nees; Instituto de Histología y Embriología “Dr. Mario H. Burgos”; Biocell; 25; 2; 12-2001; 131-1381667-57460327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:11:21Zoai:ri.conicet.gov.ar:11336/45111instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:11:21.717CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Embryogenic cell suspensions from different explants and cultivars of Eragrostis curvula (Schrad.) Nees
title Embryogenic cell suspensions from different explants and cultivars of Eragrostis curvula (Schrad.) Nees
spellingShingle Embryogenic cell suspensions from different explants and cultivars of Eragrostis curvula (Schrad.) Nees
Echenique, Carmen Viviana
PLANT TISSUE CULTURE
CELL SUSPENSIONS
GRAMINEAE
PLANT REGENERATION
Eragrostis curvula
title_short Embryogenic cell suspensions from different explants and cultivars of Eragrostis curvula (Schrad.) Nees
title_full Embryogenic cell suspensions from different explants and cultivars of Eragrostis curvula (Schrad.) Nees
title_fullStr Embryogenic cell suspensions from different explants and cultivars of Eragrostis curvula (Schrad.) Nees
title_full_unstemmed Embryogenic cell suspensions from different explants and cultivars of Eragrostis curvula (Schrad.) Nees
title_sort Embryogenic cell suspensions from different explants and cultivars of Eragrostis curvula (Schrad.) Nees
dc.creator.none.fl_str_mv Echenique, Carmen Viviana
Díaz, Marina
Polci, Pablo Aldo
Mroginski, Luis Amado
author Echenique, Carmen Viviana
author_facet Echenique, Carmen Viviana
Díaz, Marina
Polci, Pablo Aldo
Mroginski, Luis Amado
author_role author
author2 Díaz, Marina
Polci, Pablo Aldo
Mroginski, Luis Amado
author2_role author
author
author
dc.subject.none.fl_str_mv PLANT TISSUE CULTURE
CELL SUSPENSIONS
GRAMINEAE
PLANT REGENERATION
Eragrostis curvula
topic PLANT TISSUE CULTURE
CELL SUSPENSIONS
GRAMINEAE
PLANT REGENERATION
Eragrostis curvula
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this work was the establishment of embryogenic calli and cell suspensions from different explants and cultivars ofweeping lovegrass, Eragrostis curvula (Schrad.) Nees, to be used as targets for biolistic transformation. Calli were initiated from immature inflorescences, seeds, embryos, leafbases and root tips. Modified MS medium (Murashige and Skoog, 1962) was used for calli induction and proliferation. Cell suspensions were established and maintained in AAF medium (Wang et al., 1993). Morphogenic calli, embryogenic cell suspensions of moderate growth rate - consisting mainly of compact proembryogenic cell c1usters- and green plants were obtained from all the explants and cultivars assayed, except root tips. Both, explant and genotype were very important factors to be considered in order to obtain a morphogenic response and to establish cell suspensions from this grass. The statistical analysis detected interaction between both factors, explants and genotypes. Immature inflorescences were the best source of explant and Kromdraai was the cultivar that showed the best morphogenic response (expressed as the percentage of calli/explant and the percentage of calli with green spots -every green spot developed into green plants-) with inflorescences, embryos and leafbases. For Morpa and Don Pablo embryos as explants were less responsive than seeds and leafbases. There were no differences in leafbases for all the three cultivars analysed.
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Díaz, Marina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Polci, Pablo Aldo. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
description The aim of this work was the establishment of embryogenic calli and cell suspensions from different explants and cultivars ofweeping lovegrass, Eragrostis curvula (Schrad.) Nees, to be used as targets for biolistic transformation. Calli were initiated from immature inflorescences, seeds, embryos, leafbases and root tips. Modified MS medium (Murashige and Skoog, 1962) was used for calli induction and proliferation. Cell suspensions were established and maintained in AAF medium (Wang et al., 1993). Morphogenic calli, embryogenic cell suspensions of moderate growth rate - consisting mainly of compact proembryogenic cell c1usters- and green plants were obtained from all the explants and cultivars assayed, except root tips. Both, explant and genotype were very important factors to be considered in order to obtain a morphogenic response and to establish cell suspensions from this grass. The statistical analysis detected interaction between both factors, explants and genotypes. Immature inflorescences were the best source of explant and Kromdraai was the cultivar that showed the best morphogenic response (expressed as the percentage of calli/explant and the percentage of calli with green spots -every green spot developed into green plants-) with inflorescences, embryos and leafbases. For Morpa and Don Pablo embryos as explants were less responsive than seeds and leafbases. There were no differences in leafbases for all the three cultivars analysed.
publishDate 2001
dc.date.none.fl_str_mv 2001-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/45111
Echenique, Carmen Viviana; Díaz, Marina; Polci, Pablo Aldo; Mroginski, Luis Amado; Embryogenic cell suspensions from different explants and cultivars of Eragrostis curvula (Schrad.) Nees; Instituto de Histología y Embriología “Dr. Mario H. Burgos”; Biocell; 25; 2; 12-2001; 131-138
1667-5746
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/45111
identifier_str_mv Echenique, Carmen Viviana; Díaz, Marina; Polci, Pablo Aldo; Mroginski, Luis Amado; Embryogenic cell suspensions from different explants and cultivars of Eragrostis curvula (Schrad.) Nees; Instituto de Histología y Embriología “Dr. Mario H. Burgos”; Biocell; 25; 2; 12-2001; 131-138
1667-5746
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
publisher.none.fl_str_mv Instituto de Histología y Embriología “Dr. Mario H. Burgos”
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980580930617344
score 13.004268

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