Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats

Autores
Rossi, Andrea Silvana; Oliva, Maria Eugenia; Ferreira, María R.; Chicco, Adriana Graciela; Lombardo, Yolanda B.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study analyses the effect of dietary chia seed rich in n-3 a-linolenic acid on the mechanisms underlying dyslipidaemia and liver steatosis developed in rats fed a sucrose-rich diet (SRD) for either 3 weeks or 5 months. The key hepatic enzyme activities such as fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), glucose-6-phosphate dehydrogenase (G-6-PDH), carnitine palmitoyltransferase-1 (CPT-1) and fatty acid oxidase (FAO) involved in lipid metabolism and the protein mass levels of sterol regulatory element-binding protein-1 (SREBP-1) and PPARa were studied. (1) For 3 weeks, Wistar rats were fed either a SRD with 11% of maize oil (MO) as dietary fat or a SRD in which chia seed replaced MO (SRD þ Chia). (2) A second group of rats were fed a SRD for 3 months. Afterwards, half the rats continued with the SRD while for the other half, MO was replaced by chia for 2 months (SRD þ Chia). In a control group, maize starch replaced sucrose. Liver TAG and the aforementioned parameters were analysed in all groups. The replacement of MO by chia in the SRD prevented (3 weeks) or improved/normalised (5 months) increases in dyslipidaemia, liver TAG, FAS, ACC and G-6-PDH activities, and increased FAO and CPT-1 activities. Protein levels of PPARa increased, and the increased mature form of SREBP-1 protein levels in the SRD was normalised by chia in both protocols (1 and 2). The present study provides new data regarding some key mechanisms related to the fate of hepatic fatty acid metabolism that seem to be involved in the effect of dietary chia seed in preventing and normalising/ improving dyslipidaemia and liver steatosis in an insulin-resistant rat model.
Fil: Rossi, Andrea Silvana. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; Argentina
Fil: Oliva, Maria Eugenia. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; Argentina
Fil: Ferreira, María R.. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; Argentina
Fil: Chicco, Adriana Graciela. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
Fil: Lombardo, Yolanda B.. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; Argentina
Materia
Semilla de Chia Dietaria
Dislipidemia
Ppar Alfa
Srebp-1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6516
Acceder
id CONICETDig_4a20daf9a335237abe8323f574065180
oai_identifier_str oai:ri.conicet.gov.ar:11336/6516
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant ratsRossi, Andrea SilvanaOliva, Maria EugeniaFerreira, María R.Chicco, Adriana GracielaLombardo, Yolanda B.Semilla de Chia DietariaDislipidemiaPpar AlfaSrebp-1https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The present study analyses the effect of dietary chia seed rich in n-3 a-linolenic acid on the mechanisms underlying dyslipidaemia and liver steatosis developed in rats fed a sucrose-rich diet (SRD) for either 3 weeks or 5 months. The key hepatic enzyme activities such as fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), glucose-6-phosphate dehydrogenase (G-6-PDH), carnitine palmitoyltransferase-1 (CPT-1) and fatty acid oxidase (FAO) involved in lipid metabolism and the protein mass levels of sterol regulatory element-binding protein-1 (SREBP-1) and PPARa were studied. (1) For 3 weeks, Wistar rats were fed either a SRD with 11% of maize oil (MO) as dietary fat or a SRD in which chia seed replaced MO (SRD þ Chia). (2) A second group of rats were fed a SRD for 3 months. Afterwards, half the rats continued with the SRD while for the other half, MO was replaced by chia for 2 months (SRD þ Chia). In a control group, maize starch replaced sucrose. Liver TAG and the aforementioned parameters were analysed in all groups. The replacement of MO by chia in the SRD prevented (3 weeks) or improved/normalised (5 months) increases in dyslipidaemia, liver TAG, FAS, ACC and G-6-PDH activities, and increased FAO and CPT-1 activities. Protein levels of PPARa increased, and the increased mature form of SREBP-1 protein levels in the SRD was normalised by chia in both protocols (1 and 2). The present study provides new data regarding some key mechanisms related to the fate of hepatic fatty acid metabolism that seem to be involved in the effect of dietary chia seed in preventing and normalising/ improving dyslipidaemia and liver steatosis in an insulin-resistant rat model.Fil: Rossi, Andrea Silvana. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; ArgentinaFil: Oliva, Maria Eugenia. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; ArgentinaFil: Ferreira, María R.. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; ArgentinaFil: Chicco, Adriana Graciela. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; ArgentinaFil: Lombardo, Yolanda B.. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; ArgentinaCambridge University Press2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6516Rossi, Andrea Silvana; Oliva, Maria Eugenia; Ferreira, María R.; Chicco, Adriana Graciela; Lombardo, Yolanda B.; Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats; Cambridge University Press; British Journal of Nutrition; 109; 9; 5-2013; 1617-16270007-1145enginfo:eu-repo/semantics/altIdentifier/url/http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8904742&fileId=S0007114512003558info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1017/S0007114512003558info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:51:20Zoai:ri.conicet.gov.ar:11336/6516instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:51:21.163CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats
title Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats
spellingShingle Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats
Rossi, Andrea Silvana
Semilla de Chia Dietaria
Dislipidemia
Ppar Alfa
Srebp-1
title_short Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats
title_full Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats
title_fullStr Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats
title_full_unstemmed Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats
title_sort Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats
dc.creator.none.fl_str_mv Rossi, Andrea Silvana
Oliva, Maria Eugenia
Ferreira, María R.
Chicco, Adriana Graciela
Lombardo, Yolanda B.
author Rossi, Andrea Silvana
author_facet Rossi, Andrea Silvana
Oliva, Maria Eugenia
Ferreira, María R.
Chicco, Adriana Graciela
Lombardo, Yolanda B.
author_role author
author2 Oliva, Maria Eugenia
Ferreira, María R.
Chicco, Adriana Graciela
Lombardo, Yolanda B.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Semilla de Chia Dietaria
Dislipidemia
Ppar Alfa
Srebp-1
topic Semilla de Chia Dietaria
Dislipidemia
Ppar Alfa
Srebp-1
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The present study analyses the effect of dietary chia seed rich in n-3 a-linolenic acid on the mechanisms underlying dyslipidaemia and liver steatosis developed in rats fed a sucrose-rich diet (SRD) for either 3 weeks or 5 months. The key hepatic enzyme activities such as fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), glucose-6-phosphate dehydrogenase (G-6-PDH), carnitine palmitoyltransferase-1 (CPT-1) and fatty acid oxidase (FAO) involved in lipid metabolism and the protein mass levels of sterol regulatory element-binding protein-1 (SREBP-1) and PPARa were studied. (1) For 3 weeks, Wistar rats were fed either a SRD with 11% of maize oil (MO) as dietary fat or a SRD in which chia seed replaced MO (SRD þ Chia). (2) A second group of rats were fed a SRD for 3 months. Afterwards, half the rats continued with the SRD while for the other half, MO was replaced by chia for 2 months (SRD þ Chia). In a control group, maize starch replaced sucrose. Liver TAG and the aforementioned parameters were analysed in all groups. The replacement of MO by chia in the SRD prevented (3 weeks) or improved/normalised (5 months) increases in dyslipidaemia, liver TAG, FAS, ACC and G-6-PDH activities, and increased FAO and CPT-1 activities. Protein levels of PPARa increased, and the increased mature form of SREBP-1 protein levels in the SRD was normalised by chia in both protocols (1 and 2). The present study provides new data regarding some key mechanisms related to the fate of hepatic fatty acid metabolism that seem to be involved in the effect of dietary chia seed in preventing and normalising/ improving dyslipidaemia and liver steatosis in an insulin-resistant rat model.
Fil: Rossi, Andrea Silvana. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; Argentina
Fil: Oliva, Maria Eugenia. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; Argentina
Fil: Ferreira, María R.. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; Argentina
Fil: Chicco, Adriana Graciela. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
Fil: Lombardo, Yolanda B.. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Cs.biologicas; Argentina
description The present study analyses the effect of dietary chia seed rich in n-3 a-linolenic acid on the mechanisms underlying dyslipidaemia and liver steatosis developed in rats fed a sucrose-rich diet (SRD) for either 3 weeks or 5 months. The key hepatic enzyme activities such as fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), glucose-6-phosphate dehydrogenase (G-6-PDH), carnitine palmitoyltransferase-1 (CPT-1) and fatty acid oxidase (FAO) involved in lipid metabolism and the protein mass levels of sterol regulatory element-binding protein-1 (SREBP-1) and PPARa were studied. (1) For 3 weeks, Wistar rats were fed either a SRD with 11% of maize oil (MO) as dietary fat or a SRD in which chia seed replaced MO (SRD þ Chia). (2) A second group of rats were fed a SRD for 3 months. Afterwards, half the rats continued with the SRD while for the other half, MO was replaced by chia for 2 months (SRD þ Chia). In a control group, maize starch replaced sucrose. Liver TAG and the aforementioned parameters were analysed in all groups. The replacement of MO by chia in the SRD prevented (3 weeks) or improved/normalised (5 months) increases in dyslipidaemia, liver TAG, FAS, ACC and G-6-PDH activities, and increased FAO and CPT-1 activities. Protein levels of PPARa increased, and the increased mature form of SREBP-1 protein levels in the SRD was normalised by chia in both protocols (1 and 2). The present study provides new data regarding some key mechanisms related to the fate of hepatic fatty acid metabolism that seem to be involved in the effect of dietary chia seed in preventing and normalising/ improving dyslipidaemia and liver steatosis in an insulin-resistant rat model.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6516
Rossi, Andrea Silvana; Oliva, Maria Eugenia; Ferreira, María R.; Chicco, Adriana Graciela; Lombardo, Yolanda B.; Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats; Cambridge University Press; British Journal of Nutrition; 109; 9; 5-2013; 1617-1627
0007-1145
url http://hdl.handle.net/11336/6516
identifier_str_mv Rossi, Andrea Silvana; Oliva, Maria Eugenia; Ferreira, María R.; Chicco, Adriana Graciela; Lombardo, Yolanda B.; Dietary chia seed induced changes in hepatic transcription factors and their target lipogenic and oxidative enzyme activities in dyslipidaemic insulin-resistant rats; Cambridge University Press; British Journal of Nutrition; 109; 9; 5-2013; 1617-1627
0007-1145
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=8904742&fileId=S0007114512003558
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1017/S0007114512003558
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083039650643968
score 13.22299

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