α-Lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction associated to inflammatory conditions

Autores
Cimolai, María Cecilia; Vanasco, Virginia; Marchini, Timoteo Oscar; Magnani, Natalia Daniela; Evelson, Pablo Andrés; Alvarez, Silvia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An adequate redox status is important for maintaining mitochondrial function in inflammatory conditions. The aim of this work was to evaluate the effects of α-lipoic acid (LA) in kidney oxidative metabolism and mitochondrial function in lipopolysaccharide (LPS) treated rats. Sprague-Dawley rats (female, 45 ± 5 days old) were treated with LPS (10 mg/kg) and/or LA (100 mg/kg). LA prevented the 1.2 fold increase in NO production, decreased (30-40%) mitochondrial complex I-III and IV activities, and decreased (26%) membrane potential and cardiolipin oxidation (76%) observed in LPS-treated animals. No differences were observed in mitochondrial O2 consumption, mitochondrial complex II-III activity, and ATP production when LPS group was compared to LA+LPS group. Based on the improvement of the mitochondrial function, decreased production of mitochondrial NO and restoration of cardiolipin levels, this work provides new evidence that α-lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction.
Fil: Cimolai, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Vanasco, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Marchini, Timoteo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Magnani, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Alvarez, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Materia
Α-Lipoic Acid
Inflammation
Oxidative Metabolism
Kidney
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30634
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/30634
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling α-Lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction associated to inflammatory conditionsCimolai, María CeciliaVanasco, VirginiaMarchini, Timoteo OscarMagnani, Natalia DanielaEvelson, Pablo AndrésAlvarez, SilviaΑ-Lipoic AcidInflammationOxidative MetabolismKidneyhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3An adequate redox status is important for maintaining mitochondrial function in inflammatory conditions. The aim of this work was to evaluate the effects of α-lipoic acid (LA) in kidney oxidative metabolism and mitochondrial function in lipopolysaccharide (LPS) treated rats. Sprague-Dawley rats (female, 45 ± 5 days old) were treated with LPS (10 mg/kg) and/or LA (100 mg/kg). LA prevented the 1.2 fold increase in NO production, decreased (30-40%) mitochondrial complex I-III and IV activities, and decreased (26%) membrane potential and cardiolipin oxidation (76%) observed in LPS-treated animals. No differences were observed in mitochondrial O2 consumption, mitochondrial complex II-III activity, and ATP production when LPS group was compared to LA+LPS group. Based on the improvement of the mitochondrial function, decreased production of mitochondrial NO and restoration of cardiolipin levels, this work provides new evidence that α-lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction.Fil: Cimolai, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Vanasco, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Marchini, Timoteo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Magnani, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Alvarez, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; ArgentinaRoyal Society of Chemistry2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30634Cimolai, María Cecilia; Vanasco, Virginia; Marchini, Timoteo Oscar; Magnani, Natalia Daniela; Evelson, Pablo Andrés; et al.; α-Lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction associated to inflammatory conditions; Royal Society of Chemistry; Food and Function; 5; 12; 9-2014; 3143-31502042-64962042-650XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/C4FO00489Binfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2014/FO/C4FO00489B#!divAbstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:45Zoai:ri.conicet.gov.ar:11336/30634instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:45.515CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv α-Lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction associated to inflammatory conditions
title α-Lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction associated to inflammatory conditions
spellingShingle α-Lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction associated to inflammatory conditions
Cimolai, María Cecilia
Α-Lipoic Acid
Inflammation
Oxidative Metabolism
Kidney
title_short α-Lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction associated to inflammatory conditions
title_full α-Lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction associated to inflammatory conditions
title_fullStr α-Lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction associated to inflammatory conditions
title_full_unstemmed α-Lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction associated to inflammatory conditions
title_sort α-Lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction associated to inflammatory conditions
dc.creator.none.fl_str_mv Cimolai, María Cecilia
Vanasco, Virginia
Marchini, Timoteo Oscar
Magnani, Natalia Daniela
Evelson, Pablo Andrés
Alvarez, Silvia
author Cimolai, María Cecilia
author_facet Cimolai, María Cecilia
Vanasco, Virginia
Marchini, Timoteo Oscar
Magnani, Natalia Daniela
Evelson, Pablo Andrés
Alvarez, Silvia
author_role author
author2 Vanasco, Virginia
Marchini, Timoteo Oscar
Magnani, Natalia Daniela
Evelson, Pablo Andrés
Alvarez, Silvia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Α-Lipoic Acid
Inflammation
Oxidative Metabolism
Kidney
topic Α-Lipoic Acid
Inflammation
Oxidative Metabolism
Kidney
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv An adequate redox status is important for maintaining mitochondrial function in inflammatory conditions. The aim of this work was to evaluate the effects of α-lipoic acid (LA) in kidney oxidative metabolism and mitochondrial function in lipopolysaccharide (LPS) treated rats. Sprague-Dawley rats (female, 45 ± 5 days old) were treated with LPS (10 mg/kg) and/or LA (100 mg/kg). LA prevented the 1.2 fold increase in NO production, decreased (30-40%) mitochondrial complex I-III and IV activities, and decreased (26%) membrane potential and cardiolipin oxidation (76%) observed in LPS-treated animals. No differences were observed in mitochondrial O2 consumption, mitochondrial complex II-III activity, and ATP production when LPS group was compared to LA+LPS group. Based on the improvement of the mitochondrial function, decreased production of mitochondrial NO and restoration of cardiolipin levels, this work provides new evidence that α-lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction.
Fil: Cimolai, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Vanasco, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Marchini, Timoteo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Magnani, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Alvarez, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Bioquímica y Medicina Molecular; Argentina
description An adequate redox status is important for maintaining mitochondrial function in inflammatory conditions. The aim of this work was to evaluate the effects of α-lipoic acid (LA) in kidney oxidative metabolism and mitochondrial function in lipopolysaccharide (LPS) treated rats. Sprague-Dawley rats (female, 45 ± 5 days old) were treated with LPS (10 mg/kg) and/or LA (100 mg/kg). LA prevented the 1.2 fold increase in NO production, decreased (30-40%) mitochondrial complex I-III and IV activities, and decreased (26%) membrane potential and cardiolipin oxidation (76%) observed in LPS-treated animals. No differences were observed in mitochondrial O2 consumption, mitochondrial complex II-III activity, and ATP production when LPS group was compared to LA+LPS group. Based on the improvement of the mitochondrial function, decreased production of mitochondrial NO and restoration of cardiolipin levels, this work provides new evidence that α-lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30634
Cimolai, María Cecilia; Vanasco, Virginia; Marchini, Timoteo Oscar; Magnani, Natalia Daniela; Evelson, Pablo Andrés; et al.; α-Lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction associated to inflammatory conditions; Royal Society of Chemistry; Food and Function; 5; 12; 9-2014; 3143-3150
2042-6496
2042-650X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30634
identifier_str_mv Cimolai, María Cecilia; Vanasco, Virginia; Marchini, Timoteo Oscar; Magnani, Natalia Daniela; Evelson, Pablo Andrés; et al.; α-Lipoic acid protects kidney from oxidative stress and mitochondrial dysfunction associated to inflammatory conditions; Royal Society of Chemistry; Food and Function; 5; 12; 9-2014; 3143-3150
2042-6496
2042-650X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/C4FO00489B
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2014/FO/C4FO00489B#!divAbstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
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application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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