Role of regulatory and angiogenic cytokines in invasion of trophoblastic cells

Autores
Dubinsky, Valeria Rosalía; Poehlmann, Tobias G.; Pankaj, Suman; Gentile, Maria Teresa; Markert, Udo R.; Gutierrez, Gabriela Lidia
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
PROBLEM: Trophoblast invasion is a temporally and locally restricted process, which regulates implantation and oxygen arrival to the embryo through the dialog with spiral artery endothelium. Trophoblast factors with angiogenic potential are activated by hypoxia. Their capacities to induce proliferation, migration, and invasion of trophoblastic cells have been investigated. METHOD OF STUDY: The expression of interleukin (IL)-6, CD126, CD130, vascular endothelial growth factor (VEGF), and hypoxia inducible factor-1alpha (HIF-1alpha) has been silenced in JEG-3 choriocarcinoma cells by using siRNA. Silencing efficacy has been assessed by ELISA, PCR or Western blotting. Proliferation has been measured by flow cytometry, migration by a transwell assay, and invasion by a Matrigel assay. RESULTS: Proliferation was significantly reduced by silencing of CD126 or CD130, migration by silencing of IL-6, VEGF, or HIF-1alpha, and invasion by silencing of IL-6 and HIF-1alpha. CONCLUSION: The expression of IL-6, VEGF, and HIF-1alpha in trophoblastic cells is involved in the control of trophoblast invasion and migration.
Fil: Dubinsky, Valeria Rosalía. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad "juan Agustin Maza". Facultad de Farmacia y Bioquimica; Argentina. Universitat Jena; Alemania
Fil: Poehlmann, Tobias G.. Universitat Jena; Alemania
Fil: Pankaj, Suman. Aruna Asaf Ali Marg. National Institute of Immunology. Reproductive Cell Biology Laboratory; India
Fil: Gentile, Maria Teresa. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Markert, Udo R.. Universitat Jena; Alemania
Fil: Gutierrez, Gabriela Lidia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universitat Jena; Alemania
Materia
Hif-1a
Il-6
Implantation
Sirna
Vegf
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13894
Acceder
id CONICETDig_495dc906eebdad1ddac38f4d37f9f6fd
oai_identifier_str oai:ri.conicet.gov.ar:11336/13894
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of regulatory and angiogenic cytokines in invasion of trophoblastic cellsDubinsky, Valeria RosalíaPoehlmann, Tobias G.Pankaj, SumanGentile, Maria TeresaMarkert, Udo R.Gutierrez, Gabriela LidiaHif-1aIl-6ImplantationSirnaVegfhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3PROBLEM: Trophoblast invasion is a temporally and locally restricted process, which regulates implantation and oxygen arrival to the embryo through the dialog with spiral artery endothelium. Trophoblast factors with angiogenic potential are activated by hypoxia. Their capacities to induce proliferation, migration, and invasion of trophoblastic cells have been investigated. METHOD OF STUDY: The expression of interleukin (IL)-6, CD126, CD130, vascular endothelial growth factor (VEGF), and hypoxia inducible factor-1alpha (HIF-1alpha) has been silenced in JEG-3 choriocarcinoma cells by using siRNA. Silencing efficacy has been assessed by ELISA, PCR or Western blotting. Proliferation has been measured by flow cytometry, migration by a transwell assay, and invasion by a Matrigel assay. RESULTS: Proliferation was significantly reduced by silencing of CD126 or CD130, migration by silencing of IL-6, VEGF, or HIF-1alpha, and invasion by silencing of IL-6 and HIF-1alpha. CONCLUSION: The expression of IL-6, VEGF, and HIF-1alpha in trophoblastic cells is involved in the control of trophoblast invasion and migration.Fil: Dubinsky, Valeria Rosalía. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad "juan Agustin Maza". Facultad de Farmacia y Bioquimica; Argentina. Universitat Jena; AlemaniaFil: Poehlmann, Tobias G.. Universitat Jena; AlemaniaFil: Pankaj, Suman. Aruna Asaf Ali Marg. National Institute of Immunology. Reproductive Cell Biology Laboratory; IndiaFil: Gentile, Maria Teresa. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Markert, Udo R.. Universitat Jena; AlemaniaFil: Gutierrez, Gabriela Lidia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universitat Jena; AlemaniaWiley2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13894Dubinsky, Valeria Rosalía; Poehlmann, Tobias G.; Pankaj, Suman; Gentile, Maria Teresa; Markert, Udo R.; et al.; Role of regulatory and angiogenic cytokines in invasion of trophoblastic cells; Wiley; American Journal Of Reproductive Immunology; 63; 3; 3-2010; 193-1991046-7408enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0897.2009.00778.x/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1600-0897.2009.00778.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:20:14Zoai:ri.conicet.gov.ar:11336/13894instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:20:14.566CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of regulatory and angiogenic cytokines in invasion of trophoblastic cells
title Role of regulatory and angiogenic cytokines in invasion of trophoblastic cells
spellingShingle Role of regulatory and angiogenic cytokines in invasion of trophoblastic cells
Dubinsky, Valeria Rosalía
Hif-1a
Il-6
Implantation
Sirna
Vegf
title_short Role of regulatory and angiogenic cytokines in invasion of trophoblastic cells
title_full Role of regulatory and angiogenic cytokines in invasion of trophoblastic cells
title_fullStr Role of regulatory and angiogenic cytokines in invasion of trophoblastic cells
title_full_unstemmed Role of regulatory and angiogenic cytokines in invasion of trophoblastic cells
title_sort Role of regulatory and angiogenic cytokines in invasion of trophoblastic cells
dc.creator.none.fl_str_mv Dubinsky, Valeria Rosalía
Poehlmann, Tobias G.
Pankaj, Suman
Gentile, Maria Teresa
Markert, Udo R.
Gutierrez, Gabriela Lidia
author Dubinsky, Valeria Rosalía
author_facet Dubinsky, Valeria Rosalía
Poehlmann, Tobias G.
Pankaj, Suman
Gentile, Maria Teresa
Markert, Udo R.
Gutierrez, Gabriela Lidia
author_role author
author2 Poehlmann, Tobias G.
Pankaj, Suman
Gentile, Maria Teresa
Markert, Udo R.
Gutierrez, Gabriela Lidia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Hif-1a
Il-6
Implantation
Sirna
Vegf
topic Hif-1a
Il-6
Implantation
Sirna
Vegf
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv PROBLEM: Trophoblast invasion is a temporally and locally restricted process, which regulates implantation and oxygen arrival to the embryo through the dialog with spiral artery endothelium. Trophoblast factors with angiogenic potential are activated by hypoxia. Their capacities to induce proliferation, migration, and invasion of trophoblastic cells have been investigated. METHOD OF STUDY: The expression of interleukin (IL)-6, CD126, CD130, vascular endothelial growth factor (VEGF), and hypoxia inducible factor-1alpha (HIF-1alpha) has been silenced in JEG-3 choriocarcinoma cells by using siRNA. Silencing efficacy has been assessed by ELISA, PCR or Western blotting. Proliferation has been measured by flow cytometry, migration by a transwell assay, and invasion by a Matrigel assay. RESULTS: Proliferation was significantly reduced by silencing of CD126 or CD130, migration by silencing of IL-6, VEGF, or HIF-1alpha, and invasion by silencing of IL-6 and HIF-1alpha. CONCLUSION: The expression of IL-6, VEGF, and HIF-1alpha in trophoblastic cells is involved in the control of trophoblast invasion and migration.
Fil: Dubinsky, Valeria Rosalía. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad "juan Agustin Maza". Facultad de Farmacia y Bioquimica; Argentina. Universitat Jena; Alemania
Fil: Poehlmann, Tobias G.. Universitat Jena; Alemania
Fil: Pankaj, Suman. Aruna Asaf Ali Marg. National Institute of Immunology. Reproductive Cell Biology Laboratory; India
Fil: Gentile, Maria Teresa. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Markert, Udo R.. Universitat Jena; Alemania
Fil: Gutierrez, Gabriela Lidia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral "Profesor R. A. Margni"; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universitat Jena; Alemania
description PROBLEM: Trophoblast invasion is a temporally and locally restricted process, which regulates implantation and oxygen arrival to the embryo through the dialog with spiral artery endothelium. Trophoblast factors with angiogenic potential are activated by hypoxia. Their capacities to induce proliferation, migration, and invasion of trophoblastic cells have been investigated. METHOD OF STUDY: The expression of interleukin (IL)-6, CD126, CD130, vascular endothelial growth factor (VEGF), and hypoxia inducible factor-1alpha (HIF-1alpha) has been silenced in JEG-3 choriocarcinoma cells by using siRNA. Silencing efficacy has been assessed by ELISA, PCR or Western blotting. Proliferation has been measured by flow cytometry, migration by a transwell assay, and invasion by a Matrigel assay. RESULTS: Proliferation was significantly reduced by silencing of CD126 or CD130, migration by silencing of IL-6, VEGF, or HIF-1alpha, and invasion by silencing of IL-6 and HIF-1alpha. CONCLUSION: The expression of IL-6, VEGF, and HIF-1alpha in trophoblastic cells is involved in the control of trophoblast invasion and migration.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13894
Dubinsky, Valeria Rosalía; Poehlmann, Tobias G.; Pankaj, Suman; Gentile, Maria Teresa; Markert, Udo R.; et al.; Role of regulatory and angiogenic cytokines in invasion of trophoblastic cells; Wiley; American Journal Of Reproductive Immunology; 63; 3; 3-2010; 193-199
1046-7408
url http://hdl.handle.net/11336/13894
identifier_str_mv Dubinsky, Valeria Rosalía; Poehlmann, Tobias G.; Pankaj, Suman; Gentile, Maria Teresa; Markert, Udo R.; et al.; Role of regulatory and angiogenic cytokines in invasion of trophoblastic cells; Wiley; American Journal Of Reproductive Immunology; 63; 3; 3-2010; 193-199
1046-7408
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0897.2009.00778.x/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1600-0897.2009.00778.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083351138533376
score 13.22299

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