Archaea Isolates from five hypersaline environments in Argentinean Puna

Autores
Maldonado, Marcos Javier; Farias, Maria Eugenia
Año de publicación
2012
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The objective of this study was isolate and study halophilic archaea in five hypersaline environments from the Argentinean Puna. Puna is a unique natural area covering northern Chile, western Bolivia, central and southern Peru and north-western Argentina (NOA). These environments are characterized by extreme conditions where salt concentration is very high, near saturation (up to 4 M or 25%), low oxygen tension, large daily air temperature fluctuations, ranging from 20°C during the day to −40°C at night, high UV incidence, high arsenic concentrations and oligotrophy. There is not much information about halophilic archaea in Argentina. The water samples were collected from five different wetlands: Antofalla lake (Catamarca), Socompa lake (Salta), Tolar Grande “Sea eye” (Salta), Pocitos salt flat (Salta) and Llullaillaco salt flat (Salta). These lakes are located above 3500 masl. The samples were cultivated in 6 ws medium for 7 days at 37ºC. 32 strains were isolates. The isolates corresponded to genera Halorubrum, Haloarcula and Halomicrobium. Pigments from the isolates were extracted with methanol. The identification of pigments was based on absorbance spectra. The absorbance was measured at wavelength range 200-800 nm. The most were carotenoids pigments with the highest absorption peak at 492 nm. Plasmid DNA was isolated by using a modified method of Anderson and McKay and the QIAprep® Spin Miniprep Kit (QIAGEN). Ten of the isolates contain plasmids with sizes ranging from 1.5 kb to 5 kb approximately. The presence of plasmids in Archaea from hypersaline environments apparently is a widespread phenomenon. For UV resistance qualitative analysis were plated 5 ul of dilutions between 10-1 and 10-7 in drop form, on 1.5% agar (w/v). The plates were exposed 1 to 13 hours to UV – B light. Then the plates were incubated inverted for 7 days at 37 °C. All the strain had resistance to UV - B light.
Fil: Maldonado, Marcos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
VIII Congreso Argentino de Microbiología General
Mar del Plata
Argentina
Sociedad Argentina de Microbiología General
Materia
ARCHAEA
ISOLATE
LAKES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263541
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Archaea Isolates from five hypersaline environments in Argentinean PunaMaldonado, Marcos JavierFarias, Maria EugeniaARCHAEAISOLATELAKEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The objective of this study was isolate and study halophilic archaea in five hypersaline environments from the Argentinean Puna. Puna is a unique natural area covering northern Chile, western Bolivia, central and southern Peru and north-western Argentina (NOA). These environments are characterized by extreme conditions where salt concentration is very high, near saturation (up to 4 M or 25%), low oxygen tension, large daily air temperature fluctuations, ranging from 20°C during the day to −40°C at night, high UV incidence, high arsenic concentrations and oligotrophy. There is not much information about halophilic archaea in Argentina. The water samples were collected from five different wetlands: Antofalla lake (Catamarca), Socompa lake (Salta), Tolar Grande “Sea eye” (Salta), Pocitos salt flat (Salta) and Llullaillaco salt flat (Salta). These lakes are located above 3500 masl. The samples were cultivated in 6 ws medium for 7 days at 37ºC. 32 strains were isolates. The isolates corresponded to genera Halorubrum, Haloarcula and Halomicrobium. Pigments from the isolates were extracted with methanol. The identification of pigments was based on absorbance spectra. The absorbance was measured at wavelength range 200-800 nm. The most were carotenoids pigments with the highest absorption peak at 492 nm. Plasmid DNA was isolated by using a modified method of Anderson and McKay and the QIAprep® Spin Miniprep Kit (QIAGEN). Ten of the isolates contain plasmids with sizes ranging from 1.5 kb to 5 kb approximately. The presence of plasmids in Archaea from hypersaline environments apparently is a widespread phenomenon. For UV resistance qualitative analysis were plated 5 ul of dilutions between 10-1 and 10-7 in drop form, on 1.5% agar (w/v). The plates were exposed 1 to 13 hours to UV – B light. Then the plates were incubated inverted for 7 days at 37 °C. All the strain had resistance to UV - B light.Fil: Maldonado, Marcos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaVIII Congreso Argentino de Microbiología GeneralMar del PlataArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263541Archaea Isolates from five hypersaline environments in Argentinean Puna; VIII Congreso Argentino de Microbiología General; Mar del Plata; Argentina; 2012; 140-140CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/congresos-anteriores/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:10Zoai:ri.conicet.gov.ar:11336/263541instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:10.95CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Archaea Isolates from five hypersaline environments in Argentinean Puna
title Archaea Isolates from five hypersaline environments in Argentinean Puna
spellingShingle Archaea Isolates from five hypersaline environments in Argentinean Puna
Maldonado, Marcos Javier
ARCHAEA
ISOLATE
LAKES
title_short Archaea Isolates from five hypersaline environments in Argentinean Puna
title_full Archaea Isolates from five hypersaline environments in Argentinean Puna
title_fullStr Archaea Isolates from five hypersaline environments in Argentinean Puna
title_full_unstemmed Archaea Isolates from five hypersaline environments in Argentinean Puna
title_sort Archaea Isolates from five hypersaline environments in Argentinean Puna
dc.creator.none.fl_str_mv Maldonado, Marcos Javier
Farias, Maria Eugenia
author Maldonado, Marcos Javier
author_facet Maldonado, Marcos Javier
Farias, Maria Eugenia
author_role author
author2 Farias, Maria Eugenia
author2_role author
dc.subject.none.fl_str_mv ARCHAEA
ISOLATE
LAKES
topic ARCHAEA
ISOLATE
LAKES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The objective of this study was isolate and study halophilic archaea in five hypersaline environments from the Argentinean Puna. Puna is a unique natural area covering northern Chile, western Bolivia, central and southern Peru and north-western Argentina (NOA). These environments are characterized by extreme conditions where salt concentration is very high, near saturation (up to 4 M or 25%), low oxygen tension, large daily air temperature fluctuations, ranging from 20°C during the day to −40°C at night, high UV incidence, high arsenic concentrations and oligotrophy. There is not much information about halophilic archaea in Argentina. The water samples were collected from five different wetlands: Antofalla lake (Catamarca), Socompa lake (Salta), Tolar Grande “Sea eye” (Salta), Pocitos salt flat (Salta) and Llullaillaco salt flat (Salta). These lakes are located above 3500 masl. The samples were cultivated in 6 ws medium for 7 days at 37ºC. 32 strains were isolates. The isolates corresponded to genera Halorubrum, Haloarcula and Halomicrobium. Pigments from the isolates were extracted with methanol. The identification of pigments was based on absorbance spectra. The absorbance was measured at wavelength range 200-800 nm. The most were carotenoids pigments with the highest absorption peak at 492 nm. Plasmid DNA was isolated by using a modified method of Anderson and McKay and the QIAprep® Spin Miniprep Kit (QIAGEN). Ten of the isolates contain plasmids with sizes ranging from 1.5 kb to 5 kb approximately. The presence of plasmids in Archaea from hypersaline environments apparently is a widespread phenomenon. For UV resistance qualitative analysis were plated 5 ul of dilutions between 10-1 and 10-7 in drop form, on 1.5% agar (w/v). The plates were exposed 1 to 13 hours to UV – B light. Then the plates were incubated inverted for 7 days at 37 °C. All the strain had resistance to UV - B light.
Fil: Maldonado, Marcos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
VIII Congreso Argentino de Microbiología General
Mar del Plata
Argentina
Sociedad Argentina de Microbiología General
description The objective of this study was isolate and study halophilic archaea in five hypersaline environments from the Argentinean Puna. Puna is a unique natural area covering northern Chile, western Bolivia, central and southern Peru and north-western Argentina (NOA). These environments are characterized by extreme conditions where salt concentration is very high, near saturation (up to 4 M or 25%), low oxygen tension, large daily air temperature fluctuations, ranging from 20°C during the day to −40°C at night, high UV incidence, high arsenic concentrations and oligotrophy. There is not much information about halophilic archaea in Argentina. The water samples were collected from five different wetlands: Antofalla lake (Catamarca), Socompa lake (Salta), Tolar Grande “Sea eye” (Salta), Pocitos salt flat (Salta) and Llullaillaco salt flat (Salta). These lakes are located above 3500 masl. The samples were cultivated in 6 ws medium for 7 days at 37ºC. 32 strains were isolates. The isolates corresponded to genera Halorubrum, Haloarcula and Halomicrobium. Pigments from the isolates were extracted with methanol. The identification of pigments was based on absorbance spectra. The absorbance was measured at wavelength range 200-800 nm. The most were carotenoids pigments with the highest absorption peak at 492 nm. Plasmid DNA was isolated by using a modified method of Anderson and McKay and the QIAprep® Spin Miniprep Kit (QIAGEN). Ten of the isolates contain plasmids with sizes ranging from 1.5 kb to 5 kb approximately. The presence of plasmids in Archaea from hypersaline environments apparently is a widespread phenomenon. For UV resistance qualitative analysis were plated 5 ul of dilutions between 10-1 and 10-7 in drop form, on 1.5% agar (w/v). The plates were exposed 1 to 13 hours to UV – B light. Then the plates were incubated inverted for 7 days at 37 °C. All the strain had resistance to UV - B light.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263541
Archaea Isolates from five hypersaline environments in Argentinean Puna; VIII Congreso Argentino de Microbiología General; Mar del Plata; Argentina; 2012; 140-140
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263541
identifier_str_mv Archaea Isolates from five hypersaline environments in Argentinean Puna; VIII Congreso Argentino de Microbiología General; Mar del Plata; Argentina; 2012; 140-140
CONICET Digital
CONICET
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language spa
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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