Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean Puna

Autores
Dib, Julian Rafael; Maldonado, Marcos Javier; Thürmer, Andrea; Schuldes, J.; Rolf, Daniel; Farias, Maria Eugenia
Año de publicación
2011
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Living or ‘modern’ stromatolites are mostly found in hypersaline lakes and marine lagoons with extreme conditions. The most recently found and as yet oldest living stromatolites were discovered by our lab in February 2009, in Laguna Socompa and Tolar Grande, in Salta province, Argentina; at about 4,000 m. A collection of Archaea isolated from these stromatolites has been established. Among the domain of the Archaea, haloarchaea are considered suitable for the study of archaeal genetics and a rich source of plasmids as well. The main of this work was the isolation, sequencing and bioinformatic analysis of plasmid pAT23 from Halopeiger sp. strain AT23, a haloarchaea isolated from living stromatolites. Plasmid DNA was isolated by QIAGEN Plasmid Mini Kit and sequenced by Sanger technology followed by standard bioinformatic analyses. DNA sequence of a novel plasmid pAT23 (7,843 bp) from haloarchaeal Halopeiger sp. AT23 was determined. A total of 15 open reading frames (ORFs) were identified; putative functions were assigned to 4 of them among others with no annotatable function. Regarding the replication region, one ORF was found with significant homology to the RepA of Halorubrum marismortui. Analysis of the nucleotide sequence also revealed the presence of type 1 fimbriae regulatory protein FimB as well as a protein PemK, responsible for mediating cell death through inhibiting protein synthesis through the cleavage of single-stranded RNA. The largest ORF encodes a putative RloA protein which shares homology with Lutella nitroferrum. pAT23 is a novel plasmid isolated from the halophilic archaeon Halopeiger sp. strain AT23, which has little homology with those haloarchaeal plasmids reported so far. To our knowledge this is the first report of the sequencing of a plasmid for the genus Halopeiger.
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Maldonado, Marcos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Thürmer, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Schuldes, J.. Universität Göttingen; Alemania
Fil: Rolf, Daniel. Universität Göttingen; Alemania
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
VII Congreso Argentino de Microbiología General
San Miguel de Tucumán
Argentina
Sociedad Argentina de Microbiología General
Materia
PLASMID
ARCHAEA
SEQUENCE
STROMATOLITES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263540
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean PunaDib, Julian RafaelMaldonado, Marcos JavierThürmer, AndreaSchuldes, J.Rolf, DanielFarias, Maria EugeniaPLASMIDARCHAEASEQUENCESTROMATOLITEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Living or ‘modern’ stromatolites are mostly found in hypersaline lakes and marine lagoons with extreme conditions. The most recently found and as yet oldest living stromatolites were discovered by our lab in February 2009, in Laguna Socompa and Tolar Grande, in Salta province, Argentina; at about 4,000 m. A collection of Archaea isolated from these stromatolites has been established. Among the domain of the Archaea, haloarchaea are considered suitable for the study of archaeal genetics and a rich source of plasmids as well. The main of this work was the isolation, sequencing and bioinformatic analysis of plasmid pAT23 from Halopeiger sp. strain AT23, a haloarchaea isolated from living stromatolites. Plasmid DNA was isolated by QIAGEN Plasmid Mini Kit and sequenced by Sanger technology followed by standard bioinformatic analyses. DNA sequence of a novel plasmid pAT23 (7,843 bp) from haloarchaeal Halopeiger sp. AT23 was determined. A total of 15 open reading frames (ORFs) were identified; putative functions were assigned to 4 of them among others with no annotatable function. Regarding the replication region, one ORF was found with significant homology to the RepA of Halorubrum marismortui. Analysis of the nucleotide sequence also revealed the presence of type 1 fimbriae regulatory protein FimB as well as a protein PemK, responsible for mediating cell death through inhibiting protein synthesis through the cleavage of single-stranded RNA. The largest ORF encodes a putative RloA protein which shares homology with Lutella nitroferrum. pAT23 is a novel plasmid isolated from the halophilic archaeon Halopeiger sp. strain AT23, which has little homology with those haloarchaeal plasmids reported so far. To our knowledge this is the first report of the sequencing of a plasmid for the genus Halopeiger.Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Maldonado, Marcos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Thürmer, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Schuldes, J.. Universität Göttingen; AlemaniaFil: Rolf, Daniel. Universität Göttingen; AlemaniaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaVII Congreso Argentino de Microbiología GeneralSan Miguel de TucumánArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263540Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean Puna; VII Congreso Argentino de Microbiología General; San Miguel de Tucumán; Argentina; 2011; 54-54CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/congresos-anteriores/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:07Zoai:ri.conicet.gov.ar:11336/263540instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:08.232CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean Puna
title Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean Puna
spellingShingle Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean Puna
Dib, Julian Rafael
PLASMID
ARCHAEA
SEQUENCE
STROMATOLITES
title_short Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean Puna
title_full Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean Puna
title_fullStr Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean Puna
title_full_unstemmed Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean Puna
title_sort Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean Puna
dc.creator.none.fl_str_mv Dib, Julian Rafael
Maldonado, Marcos Javier
Thürmer, Andrea
Schuldes, J.
Rolf, Daniel
Farias, Maria Eugenia
author Dib, Julian Rafael
author_facet Dib, Julian Rafael
Maldonado, Marcos Javier
Thürmer, Andrea
Schuldes, J.
Rolf, Daniel
Farias, Maria Eugenia
author_role author
author2 Maldonado, Marcos Javier
Thürmer, Andrea
Schuldes, J.
Rolf, Daniel
Farias, Maria Eugenia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv PLASMID
ARCHAEA
SEQUENCE
STROMATOLITES
topic PLASMID
ARCHAEA
SEQUENCE
STROMATOLITES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Living or ‘modern’ stromatolites are mostly found in hypersaline lakes and marine lagoons with extreme conditions. The most recently found and as yet oldest living stromatolites were discovered by our lab in February 2009, in Laguna Socompa and Tolar Grande, in Salta province, Argentina; at about 4,000 m. A collection of Archaea isolated from these stromatolites has been established. Among the domain of the Archaea, haloarchaea are considered suitable for the study of archaeal genetics and a rich source of plasmids as well. The main of this work was the isolation, sequencing and bioinformatic analysis of plasmid pAT23 from Halopeiger sp. strain AT23, a haloarchaea isolated from living stromatolites. Plasmid DNA was isolated by QIAGEN Plasmid Mini Kit and sequenced by Sanger technology followed by standard bioinformatic analyses. DNA sequence of a novel plasmid pAT23 (7,843 bp) from haloarchaeal Halopeiger sp. AT23 was determined. A total of 15 open reading frames (ORFs) were identified; putative functions were assigned to 4 of them among others with no annotatable function. Regarding the replication region, one ORF was found with significant homology to the RepA of Halorubrum marismortui. Analysis of the nucleotide sequence also revealed the presence of type 1 fimbriae regulatory protein FimB as well as a protein PemK, responsible for mediating cell death through inhibiting protein synthesis through the cleavage of single-stranded RNA. The largest ORF encodes a putative RloA protein which shares homology with Lutella nitroferrum. pAT23 is a novel plasmid isolated from the halophilic archaeon Halopeiger sp. strain AT23, which has little homology with those haloarchaeal plasmids reported so far. To our knowledge this is the first report of the sequencing of a plasmid for the genus Halopeiger.
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Maldonado, Marcos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Thürmer, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Schuldes, J.. Universität Göttingen; Alemania
Fil: Rolf, Daniel. Universität Göttingen; Alemania
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
VII Congreso Argentino de Microbiología General
San Miguel de Tucumán
Argentina
Sociedad Argentina de Microbiología General
description Living or ‘modern’ stromatolites are mostly found in hypersaline lakes and marine lagoons with extreme conditions. The most recently found and as yet oldest living stromatolites were discovered by our lab in February 2009, in Laguna Socompa and Tolar Grande, in Salta province, Argentina; at about 4,000 m. A collection of Archaea isolated from these stromatolites has been established. Among the domain of the Archaea, haloarchaea are considered suitable for the study of archaeal genetics and a rich source of plasmids as well. The main of this work was the isolation, sequencing and bioinformatic analysis of plasmid pAT23 from Halopeiger sp. strain AT23, a haloarchaea isolated from living stromatolites. Plasmid DNA was isolated by QIAGEN Plasmid Mini Kit and sequenced by Sanger technology followed by standard bioinformatic analyses. DNA sequence of a novel plasmid pAT23 (7,843 bp) from haloarchaeal Halopeiger sp. AT23 was determined. A total of 15 open reading frames (ORFs) were identified; putative functions were assigned to 4 of them among others with no annotatable function. Regarding the replication region, one ORF was found with significant homology to the RepA of Halorubrum marismortui. Analysis of the nucleotide sequence also revealed the presence of type 1 fimbriae regulatory protein FimB as well as a protein PemK, responsible for mediating cell death through inhibiting protein synthesis through the cleavage of single-stranded RNA. The largest ORF encodes a putative RloA protein which shares homology with Lutella nitroferrum. pAT23 is a novel plasmid isolated from the halophilic archaeon Halopeiger sp. strain AT23, which has little homology with those haloarchaeal plasmids reported so far. To our knowledge this is the first report of the sequencing of a plasmid for the genus Halopeiger.
publishDate 2011
dc.date.none.fl_str_mv 2011
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status_str publishedVersion
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263540
Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean Puna; VII Congreso Argentino de Microbiología General; San Miguel de Tucumán; Argentina; 2011; 54-54
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263540
identifier_str_mv Complete Nucleotide Sequence of a Novel Plasmid pAT23 from Halopiger sp. AT23 Isolated from Living Stromatolites in the Argentinean Puna; VII Congreso Argentino de Microbiología General; San Miguel de Tucumán; Argentina; 2011; 54-54
CONICET Digital
CONICET
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
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