Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments

Autores
Lamprecht Grandío, María; Cortesão, Marta; Mirete, Salvador; Benguigui de la Cámara, Macarena; de Figueras, Carolina G.; Pérez Pantoja, Danilo; White, Joseph John; Farias, Maria Eugenia; Rosselló Móra, Ramon; González Pastor, José Eduardo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microorganisms that thrive in hypersaline environments on the surface of our planet are exposed to the harmful effects of ultraviolet radiation. Therefore, for their protection, they have sunscreen pigments and highly efficient DNA repair and protection systems. The present study aimed to identify new genes involved in UV radiation resistance from these microorganisms, many of which cannot be cultured in the laboratory. Thus, a functional metagenomic approach was used and for this, small-insert libraries were constructed with DNA isolated from microorganisms of high-altitude Andean hypersaline lakes in Argentina (Diamante and Ojo Seco lakes, 4,589 and 3,200 m, respectively) and from the Es Trenc solar saltern in Spain. The libraries were hosted in a UV radiation-sensitive strain of Escherichia coli (recA mutant) and they were exposed to UVB. The resistant colonies were analyzed and as a result, four clones were identified with environmental DNA fragments containing five genes that conferred resistance to UV radiation in E. coli. One gene encoded a RecA-like protein, complementing the mutation in recA that makes the E. coli host strain more sensitive to UV radiation. Two other genes from the same DNA fragment encoded a TATA-box binding protein and an unknown protein, both responsible for UV resistance. Interestingly, two other genes from different and remote environments, the Ojo Seco Andean lake and the Es Trenc saltern, encoded two hypothetical proteins that can be considered homologous based on their significant amino acid similarity (49%). All of these genes also conferred resistance to 4-nitroquinoline 1-oxide (4-NQO), a compound that mimics the effect of UV radiation on DNA, and also to perchlorate, a powerful oxidant that can induce DNA damage. Furthermore, the hypothetical protein from the Es Trenc salterns was localized as discrete foci possibly associated with damaged sites in the DNA in cells treated with 4-NQO, so it could be involved in the repair of damaged DNA. In summary, novel genes involved in resistance to UV radiation, 4-NQO and perchlorate have been identified in this work and two of them encoding hypothetical proteins that could be involved in DNA damage repair activities not previously described.
Fil: Lamprecht Grandío, María. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; España
Fil: Cortesão, Marta. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; España
Fil: Mirete, Salvador. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; España
Fil: Benguigui de la Cámara, Macarena. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; España
Fil: de Figueras, Carolina G.. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; España
Fil: Pérez Pantoja, Danilo. Universidad Tecnológica Metropolitana; España
Fil: White, Joseph John. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; España
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Rosselló Móra, Ramon. Instituto Mediterráneo Estudios Avanzados; España
Fil: González Pastor, José Eduardo. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; España
Materia
UV
EXTREMOPHILES
ANDEAN LAKES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/111663
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/111663
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline EnvironmentsLamprecht Grandío, MaríaCortesão, MartaMirete, SalvadorBenguigui de la Cámara, Macarenade Figueras, Carolina G.Pérez Pantoja, DaniloWhite, Joseph JohnFarias, Maria EugeniaRosselló Móra, RamonGonzález Pastor, José EduardoUVEXTREMOPHILESANDEAN LAKEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Microorganisms that thrive in hypersaline environments on the surface of our planet are exposed to the harmful effects of ultraviolet radiation. Therefore, for their protection, they have sunscreen pigments and highly efficient DNA repair and protection systems. The present study aimed to identify new genes involved in UV radiation resistance from these microorganisms, many of which cannot be cultured in the laboratory. Thus, a functional metagenomic approach was used and for this, small-insert libraries were constructed with DNA isolated from microorganisms of high-altitude Andean hypersaline lakes in Argentina (Diamante and Ojo Seco lakes, 4,589 and 3,200 m, respectively) and from the Es Trenc solar saltern in Spain. The libraries were hosted in a UV radiation-sensitive strain of Escherichia coli (recA mutant) and they were exposed to UVB. The resistant colonies were analyzed and as a result, four clones were identified with environmental DNA fragments containing five genes that conferred resistance to UV radiation in E. coli. One gene encoded a RecA-like protein, complementing the mutation in recA that makes the E. coli host strain more sensitive to UV radiation. Two other genes from the same DNA fragment encoded a TATA-box binding protein and an unknown protein, both responsible for UV resistance. Interestingly, two other genes from different and remote environments, the Ojo Seco Andean lake and the Es Trenc saltern, encoded two hypothetical proteins that can be considered homologous based on their significant amino acid similarity (49%). All of these genes also conferred resistance to 4-nitroquinoline 1-oxide (4-NQO), a compound that mimics the effect of UV radiation on DNA, and also to perchlorate, a powerful oxidant that can induce DNA damage. Furthermore, the hypothetical protein from the Es Trenc salterns was localized as discrete foci possibly associated with damaged sites in the DNA in cells treated with 4-NQO, so it could be involved in the repair of damaged DNA. In summary, novel genes involved in resistance to UV radiation, 4-NQO and perchlorate have been identified in this work and two of them encoding hypothetical proteins that could be involved in DNA damage repair activities not previously described.Fil: Lamprecht Grandío, María. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; EspañaFil: Cortesão, Marta. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; EspañaFil: Mirete, Salvador. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; EspañaFil: Benguigui de la Cámara, Macarena. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; EspañaFil: de Figueras, Carolina G.. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; EspañaFil: Pérez Pantoja, Danilo. Universidad Tecnológica Metropolitana; EspañaFil: White, Joseph John. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; EspañaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Rosselló Móra, Ramon. Instituto Mediterráneo Estudios Avanzados; EspañaFil: González Pastor, José Eduardo. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; EspañaFrontiers Media S.A.2020-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/111663Lamprecht Grandío, María; Cortesão, Marta; Mirete, Salvador; Benguigui de la Cámara, Macarena; de Figueras, Carolina G.; et al.; Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments; Frontiers Media S.A.; Frontiers in Microbiology; 11; 3-20201664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2020.00453/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.00453info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7135895/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:49Zoai:ri.conicet.gov.ar:11336/111663instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:49.362CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments
title Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments
spellingShingle Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments
Lamprecht Grandío, María
UV
EXTREMOPHILES
ANDEAN LAKES
title_short Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments
title_full Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments
title_fullStr Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments
title_full_unstemmed Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments
title_sort Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments
dc.creator.none.fl_str_mv Lamprecht Grandío, María
Cortesão, Marta
Mirete, Salvador
Benguigui de la Cámara, Macarena
de Figueras, Carolina G.
Pérez Pantoja, Danilo
White, Joseph John
Farias, Maria Eugenia
Rosselló Móra, Ramon
González Pastor, José Eduardo
author Lamprecht Grandío, María
author_facet Lamprecht Grandío, María
Cortesão, Marta
Mirete, Salvador
Benguigui de la Cámara, Macarena
de Figueras, Carolina G.
Pérez Pantoja, Danilo
White, Joseph John
Farias, Maria Eugenia
Rosselló Móra, Ramon
González Pastor, José Eduardo
author_role author
author2 Cortesão, Marta
Mirete, Salvador
Benguigui de la Cámara, Macarena
de Figueras, Carolina G.
Pérez Pantoja, Danilo
White, Joseph John
Farias, Maria Eugenia
Rosselló Móra, Ramon
González Pastor, José Eduardo
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv UV
EXTREMOPHILES
ANDEAN LAKES
topic UV
EXTREMOPHILES
ANDEAN LAKES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Microorganisms that thrive in hypersaline environments on the surface of our planet are exposed to the harmful effects of ultraviolet radiation. Therefore, for their protection, they have sunscreen pigments and highly efficient DNA repair and protection systems. The present study aimed to identify new genes involved in UV radiation resistance from these microorganisms, many of which cannot be cultured in the laboratory. Thus, a functional metagenomic approach was used and for this, small-insert libraries were constructed with DNA isolated from microorganisms of high-altitude Andean hypersaline lakes in Argentina (Diamante and Ojo Seco lakes, 4,589 and 3,200 m, respectively) and from the Es Trenc solar saltern in Spain. The libraries were hosted in a UV radiation-sensitive strain of Escherichia coli (recA mutant) and they were exposed to UVB. The resistant colonies were analyzed and as a result, four clones were identified with environmental DNA fragments containing five genes that conferred resistance to UV radiation in E. coli. One gene encoded a RecA-like protein, complementing the mutation in recA that makes the E. coli host strain more sensitive to UV radiation. Two other genes from the same DNA fragment encoded a TATA-box binding protein and an unknown protein, both responsible for UV resistance. Interestingly, two other genes from different and remote environments, the Ojo Seco Andean lake and the Es Trenc saltern, encoded two hypothetical proteins that can be considered homologous based on their significant amino acid similarity (49%). All of these genes also conferred resistance to 4-nitroquinoline 1-oxide (4-NQO), a compound that mimics the effect of UV radiation on DNA, and also to perchlorate, a powerful oxidant that can induce DNA damage. Furthermore, the hypothetical protein from the Es Trenc salterns was localized as discrete foci possibly associated with damaged sites in the DNA in cells treated with 4-NQO, so it could be involved in the repair of damaged DNA. In summary, novel genes involved in resistance to UV radiation, 4-NQO and perchlorate have been identified in this work and two of them encoding hypothetical proteins that could be involved in DNA damage repair activities not previously described.
Fil: Lamprecht Grandío, María. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; España
Fil: Cortesão, Marta. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; España
Fil: Mirete, Salvador. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; España
Fil: Benguigui de la Cámara, Macarena. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; España
Fil: de Figueras, Carolina G.. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; España
Fil: Pérez Pantoja, Danilo. Universidad Tecnológica Metropolitana; España
Fil: White, Joseph John. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; España
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Rosselló Móra, Ramon. Instituto Mediterráneo Estudios Avanzados; España
Fil: González Pastor, José Eduardo. Consejo Superior de Investigaciones Cientificas. Centro de Astrobiologia.; España
description Microorganisms that thrive in hypersaline environments on the surface of our planet are exposed to the harmful effects of ultraviolet radiation. Therefore, for their protection, they have sunscreen pigments and highly efficient DNA repair and protection systems. The present study aimed to identify new genes involved in UV radiation resistance from these microorganisms, many of which cannot be cultured in the laboratory. Thus, a functional metagenomic approach was used and for this, small-insert libraries were constructed with DNA isolated from microorganisms of high-altitude Andean hypersaline lakes in Argentina (Diamante and Ojo Seco lakes, 4,589 and 3,200 m, respectively) and from the Es Trenc solar saltern in Spain. The libraries were hosted in a UV radiation-sensitive strain of Escherichia coli (recA mutant) and they were exposed to UVB. The resistant colonies were analyzed and as a result, four clones were identified with environmental DNA fragments containing five genes that conferred resistance to UV radiation in E. coli. One gene encoded a RecA-like protein, complementing the mutation in recA that makes the E. coli host strain more sensitive to UV radiation. Two other genes from the same DNA fragment encoded a TATA-box binding protein and an unknown protein, both responsible for UV resistance. Interestingly, two other genes from different and remote environments, the Ojo Seco Andean lake and the Es Trenc saltern, encoded two hypothetical proteins that can be considered homologous based on their significant amino acid similarity (49%). All of these genes also conferred resistance to 4-nitroquinoline 1-oxide (4-NQO), a compound that mimics the effect of UV radiation on DNA, and also to perchlorate, a powerful oxidant that can induce DNA damage. Furthermore, the hypothetical protein from the Es Trenc salterns was localized as discrete foci possibly associated with damaged sites in the DNA in cells treated with 4-NQO, so it could be involved in the repair of damaged DNA. In summary, novel genes involved in resistance to UV radiation, 4-NQO and perchlorate have been identified in this work and two of them encoding hypothetical proteins that could be involved in DNA damage repair activities not previously described.
publishDate 2020
dc.date.none.fl_str_mv 2020-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/111663
Lamprecht Grandío, María; Cortesão, Marta; Mirete, Salvador; Benguigui de la Cámara, Macarena; de Figueras, Carolina G.; et al.; Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments; Frontiers Media S.A.; Frontiers in Microbiology; 11; 3-2020
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/111663
identifier_str_mv Lamprecht Grandío, María; Cortesão, Marta; Mirete, Salvador; Benguigui de la Cámara, Macarena; de Figueras, Carolina G.; et al.; Novel Genes Involved in Resistance to Both Ultraviolet Radiation and Perchlorate From the Metagenomes of Hypersaline Environments; Frontiers Media S.A.; Frontiers in Microbiology; 11; 3-2020
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2020.00453/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2020.00453
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7135895/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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