Culturable organotrofic bacteria from high-altitude andean lakes

Autores
Gorriti, Marta Fabiana; Farias, Maria Eugenia
Año de publicación
2011
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The Andean Altiplano is a sedimentary volcanic plateau at about 4.000 m. Several lakes in the Altiplano are formed over evaporitic endorheic basins. These aquatic ecosystems present extreme conditions (high solar irradiance, arid conditions, low nutrient concentrations, heavy metals, large temperature fluctuations) and are a source of microorganisms with potential biotechnological applications. Lately, many studies have reported that the assessment of bacterial diversity by cultivation-independent methods is the most appropriate for studying diversity, owing to the existence of a host of unculturable. However, cultivation remains the preferred method for the acquisition of an accurate picture of the physiology and complex ecological interactions in which microorganisms engage. In this study, we attempted to determine the culturable bacterial diversity and extend the culture collections of Andean lakes in Argentina. Bacteria were isolated from Socompa and Diamante lakes in the Argentinean Puna. They were grown in different broth: LB, MGM, PY and 6WS. The pure and cellular morphology were determined by phase contrast microscopy and Gram stain. Bacterial diversity among the isolated was evaluated by PCR-amplified 16S rDNAs followed by phylogenetic analysis of 16S rDNA sequences. Twenty six strains were found. A neighbor-joining tree of the partial 16S rDNA sequences resulted in the division of the 18 strains of Socompa into two major groups, 14 strains of γ-Proteobacteria (77.7%) and 4 strains of Firmicutes (22.3%). Eigth strains of Diamante resulted in the division into 6 strains of Firmicutes (75%) and 2 strains Actinobacteria (25%). The γ-proteobacteria was group more diversity, including several genus: Salinivibrio, Shewanella, Pseudomona, Halomona, Aeromona, Idiomarina and Chromohalobacter. Additionally, seven new species candidates were found in Socompa, based on similarities of the 16S rDNA sequences to those of previously reported species.
Fil: Gorriti, Marta Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
VII Congreso Argentino de Microbiología General
Tucuman
Argentina
Sociedad Argentina de Microbiología General
Materia
BACTERIA ORGANOTROFICA
LAGUNAS DE ALTURA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194935
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/194935
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Culturable organotrofic bacteria from high-altitude andean lakesGorriti, Marta FabianaFarias, Maria EugeniaBACTERIA ORGANOTROFICALAGUNAS DE ALTURAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Andean Altiplano is a sedimentary volcanic plateau at about 4.000 m. Several lakes in the Altiplano are formed over evaporitic endorheic basins. These aquatic ecosystems present extreme conditions (high solar irradiance, arid conditions, low nutrient concentrations, heavy metals, large temperature fluctuations) and are a source of microorganisms with potential biotechnological applications. Lately, many studies have reported that the assessment of bacterial diversity by cultivation-independent methods is the most appropriate for studying diversity, owing to the existence of a host of unculturable. However, cultivation remains the preferred method for the acquisition of an accurate picture of the physiology and complex ecological interactions in which microorganisms engage. In this study, we attempted to determine the culturable bacterial diversity and extend the culture collections of Andean lakes in Argentina. Bacteria were isolated from Socompa and Diamante lakes in the Argentinean Puna. They were grown in different broth: LB, MGM, PY and 6WS. The pure and cellular morphology were determined by phase contrast microscopy and Gram stain. Bacterial diversity among the isolated was evaluated by PCR-amplified 16S rDNAs followed by phylogenetic analysis of 16S rDNA sequences. Twenty six strains were found. A neighbor-joining tree of the partial 16S rDNA sequences resulted in the division of the 18 strains of Socompa into two major groups, 14 strains of γ-Proteobacteria (77.7%) and 4 strains of Firmicutes (22.3%). Eigth strains of Diamante resulted in the division into 6 strains of Firmicutes (75%) and 2 strains Actinobacteria (25%). The γ-proteobacteria was group more diversity, including several genus: Salinivibrio, Shewanella, Pseudomona, Halomona, Aeromona, Idiomarina and Chromohalobacter. Additionally, seven new species candidates were found in Socompa, based on similarities of the 16S rDNA sequences to those of previously reported species.Fil: Gorriti, Marta Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaVII Congreso Argentino de Microbiología GeneralTucumanArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiologia General2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194935Culturable organotrofic bacteria from high-altitude andean lakes; VII Congreso Argentino de Microbiología General; Tucuman; Argentina; 2011; 33-33CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2011.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:20Zoai:ri.conicet.gov.ar:11336/194935instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:20.34CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Culturable organotrofic bacteria from high-altitude andean lakes
title Culturable organotrofic bacteria from high-altitude andean lakes
spellingShingle Culturable organotrofic bacteria from high-altitude andean lakes
Gorriti, Marta Fabiana
BACTERIA ORGANOTROFICA
LAGUNAS DE ALTURA
title_short Culturable organotrofic bacteria from high-altitude andean lakes
title_full Culturable organotrofic bacteria from high-altitude andean lakes
title_fullStr Culturable organotrofic bacteria from high-altitude andean lakes
title_full_unstemmed Culturable organotrofic bacteria from high-altitude andean lakes
title_sort Culturable organotrofic bacteria from high-altitude andean lakes
dc.creator.none.fl_str_mv Gorriti, Marta Fabiana
Farias, Maria Eugenia
author Gorriti, Marta Fabiana
author_facet Gorriti, Marta Fabiana
Farias, Maria Eugenia
author_role author
author2 Farias, Maria Eugenia
author2_role author
dc.subject.none.fl_str_mv BACTERIA ORGANOTROFICA
LAGUNAS DE ALTURA
topic BACTERIA ORGANOTROFICA
LAGUNAS DE ALTURA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Andean Altiplano is a sedimentary volcanic plateau at about 4.000 m. Several lakes in the Altiplano are formed over evaporitic endorheic basins. These aquatic ecosystems present extreme conditions (high solar irradiance, arid conditions, low nutrient concentrations, heavy metals, large temperature fluctuations) and are a source of microorganisms with potential biotechnological applications. Lately, many studies have reported that the assessment of bacterial diversity by cultivation-independent methods is the most appropriate for studying diversity, owing to the existence of a host of unculturable. However, cultivation remains the preferred method for the acquisition of an accurate picture of the physiology and complex ecological interactions in which microorganisms engage. In this study, we attempted to determine the culturable bacterial diversity and extend the culture collections of Andean lakes in Argentina. Bacteria were isolated from Socompa and Diamante lakes in the Argentinean Puna. They were grown in different broth: LB, MGM, PY and 6WS. The pure and cellular morphology were determined by phase contrast microscopy and Gram stain. Bacterial diversity among the isolated was evaluated by PCR-amplified 16S rDNAs followed by phylogenetic analysis of 16S rDNA sequences. Twenty six strains were found. A neighbor-joining tree of the partial 16S rDNA sequences resulted in the division of the 18 strains of Socompa into two major groups, 14 strains of γ-Proteobacteria (77.7%) and 4 strains of Firmicutes (22.3%). Eigth strains of Diamante resulted in the division into 6 strains of Firmicutes (75%) and 2 strains Actinobacteria (25%). The γ-proteobacteria was group more diversity, including several genus: Salinivibrio, Shewanella, Pseudomona, Halomona, Aeromona, Idiomarina and Chromohalobacter. Additionally, seven new species candidates were found in Socompa, based on similarities of the 16S rDNA sequences to those of previously reported species.
Fil: Gorriti, Marta Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
VII Congreso Argentino de Microbiología General
Tucuman
Argentina
Sociedad Argentina de Microbiología General
description The Andean Altiplano is a sedimentary volcanic plateau at about 4.000 m. Several lakes in the Altiplano are formed over evaporitic endorheic basins. These aquatic ecosystems present extreme conditions (high solar irradiance, arid conditions, low nutrient concentrations, heavy metals, large temperature fluctuations) and are a source of microorganisms with potential biotechnological applications. Lately, many studies have reported that the assessment of bacterial diversity by cultivation-independent methods is the most appropriate for studying diversity, owing to the existence of a host of unculturable. However, cultivation remains the preferred method for the acquisition of an accurate picture of the physiology and complex ecological interactions in which microorganisms engage. In this study, we attempted to determine the culturable bacterial diversity and extend the culture collections of Andean lakes in Argentina. Bacteria were isolated from Socompa and Diamante lakes in the Argentinean Puna. They were grown in different broth: LB, MGM, PY and 6WS. The pure and cellular morphology were determined by phase contrast microscopy and Gram stain. Bacterial diversity among the isolated was evaluated by PCR-amplified 16S rDNAs followed by phylogenetic analysis of 16S rDNA sequences. Twenty six strains were found. A neighbor-joining tree of the partial 16S rDNA sequences resulted in the division of the 18 strains of Socompa into two major groups, 14 strains of γ-Proteobacteria (77.7%) and 4 strains of Firmicutes (22.3%). Eigth strains of Diamante resulted in the division into 6 strains of Firmicutes (75%) and 2 strains Actinobacteria (25%). The γ-proteobacteria was group more diversity, including several genus: Salinivibrio, Shewanella, Pseudomona, Halomona, Aeromona, Idiomarina and Chromohalobacter. Additionally, seven new species candidates were found in Socompa, based on similarities of the 16S rDNA sequences to those of previously reported species.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
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info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194935
Culturable organotrofic bacteria from high-altitude andean lakes; VII Congreso Argentino de Microbiología General; Tucuman; Argentina; 2011; 33-33
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194935
identifier_str_mv Culturable organotrofic bacteria from high-altitude andean lakes; VII Congreso Argentino de Microbiología General; Tucuman; Argentina; 2011; 33-33
CONICET Digital
CONICET
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language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2011.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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