On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to th...

Autores
Toledano, Rosa; Cortés, José; Andini, Juan Carlos; Vázquez, Ana; Villén, Jesús
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present work on-line derivatization is combined with on-line normal phase liquid chromatography-gas chromatography (NPLC-GC) to analyze total sterols in edible oils. The method uses the TOTAD interface with an additional LC injection valve to automatically introduce the derivatization reagent. The derivatization reaction takes place in the adsorbent material located inside the liner of the TOTAD interface. The samples were saponified with potassium hydroxide in an ethanolic solution and the unsaponifiable fraction was extracted with diethyl ether. The extract was then analyzed by online derivatization with on-line NPLC?GC, avoiding the laborious thin layer chromatography and off-line derivatization steps used in the Official European Union (EU) method. The relative standard deviations (RSDs) from the absolute peak area were lower than 14% except for campestanol. No variability in retention time was observed. Limits of detection (LODs) were less than 8.82 mg/kg. Different edible oils were analyzed using the proposed method and the results obtained were compared with those obtained using the Official EU method. There was good agreement between both methods for all the sterols analyzed.
Fil: Toledano, Rosa. Universidad de Castilla-La Mancha; España
Fil: Cortés, José. Universidad de Castilla-La Mancha; España
Fil: Andini, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
Fil: Vázquez, Ana. Universidad de Castilla-La Mancha; España
Fil: Villén, Jesús. Universidad de Castilla-La Mancha; España
Materia
On-Line Derivatization
On-Line Nplcgc
Totad Interface
Total Sterols
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10731
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10731
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to the analysis of total sterols in edible oilsToledano, RosaCortés, JoséAndini, Juan CarlosVázquez, AnaVillén, JesúsOn-Line DerivatizationOn-Line NplcgcTotad InterfaceTotal Sterolshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In the present work on-line derivatization is combined with on-line normal phase liquid chromatography-gas chromatography (NPLC-GC) to analyze total sterols in edible oils. The method uses the TOTAD interface with an additional LC injection valve to automatically introduce the derivatization reagent. The derivatization reaction takes place in the adsorbent material located inside the liner of the TOTAD interface. The samples were saponified with potassium hydroxide in an ethanolic solution and the unsaponifiable fraction was extracted with diethyl ether. The extract was then analyzed by online derivatization with on-line NPLC?GC, avoiding the laborious thin layer chromatography and off-line derivatization steps used in the Official European Union (EU) method. The relative standard deviations (RSDs) from the absolute peak area were lower than 14% except for campestanol. No variability in retention time was observed. Limits of detection (LODs) were less than 8.82 mg/kg. Different edible oils were analyzed using the proposed method and the results obtained were compared with those obtained using the Official EU method. There was good agreement between both methods for all the sterols analyzed.Fil: Toledano, Rosa. Universidad de Castilla-La Mancha; EspañaFil: Cortés, José. Universidad de Castilla-La Mancha; EspañaFil: Andini, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); ArgentinaFil: Vázquez, Ana. Universidad de Castilla-La Mancha; EspañaFil: Villén, Jesús. Universidad de Castilla-La Mancha; EspañaElsevier Science2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10731Toledano, Rosa; Cortés, José; Andini, Juan Carlos; Vázquez, Ana; Villén, Jesús; On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to the analysis of total sterols in edible oils; Elsevier Science; Journal Of Chromatography - A; 1256; 7-2012; 191-1960021-9673enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2012.07.057info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0021967312011120info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:46Zoai:ri.conicet.gov.ar:11336/10731instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:47.049CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to the analysis of total sterols in edible oils
title On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to the analysis of total sterols in edible oils
spellingShingle On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to the analysis of total sterols in edible oils
Toledano, Rosa
On-Line Derivatization
On-Line Nplcgc
Totad Interface
Total Sterols
title_short On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to the analysis of total sterols in edible oils
title_full On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to the analysis of total sterols in edible oils
title_fullStr On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to the analysis of total sterols in edible oils
title_full_unstemmed On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to the analysis of total sterols in edible oils
title_sort On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to the analysis of total sterols in edible oils
dc.creator.none.fl_str_mv Toledano, Rosa
Cortés, José
Andini, Juan Carlos
Vázquez, Ana
Villén, Jesús
author Toledano, Rosa
author_facet Toledano, Rosa
Cortés, José
Andini, Juan Carlos
Vázquez, Ana
Villén, Jesús
author_role author
author2 Cortés, José
Andini, Juan Carlos
Vázquez, Ana
Villén, Jesús
author2_role author
author
author
author
dc.subject.none.fl_str_mv On-Line Derivatization
On-Line Nplcgc
Totad Interface
Total Sterols
topic On-Line Derivatization
On-Line Nplcgc
Totad Interface
Total Sterols
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the present work on-line derivatization is combined with on-line normal phase liquid chromatography-gas chromatography (NPLC-GC) to analyze total sterols in edible oils. The method uses the TOTAD interface with an additional LC injection valve to automatically introduce the derivatization reagent. The derivatization reaction takes place in the adsorbent material located inside the liner of the TOTAD interface. The samples were saponified with potassium hydroxide in an ethanolic solution and the unsaponifiable fraction was extracted with diethyl ether. The extract was then analyzed by online derivatization with on-line NPLC?GC, avoiding the laborious thin layer chromatography and off-line derivatization steps used in the Official European Union (EU) method. The relative standard deviations (RSDs) from the absolute peak area were lower than 14% except for campestanol. No variability in retention time was observed. Limits of detection (LODs) were less than 8.82 mg/kg. Different edible oils were analyzed using the proposed method and the results obtained were compared with those obtained using the Official EU method. There was good agreement between both methods for all the sterols analyzed.
Fil: Toledano, Rosa. Universidad de Castilla-La Mancha; España
Fil: Cortés, José. Universidad de Castilla-La Mancha; España
Fil: Andini, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina
Fil: Vázquez, Ana. Universidad de Castilla-La Mancha; España
Fil: Villén, Jesús. Universidad de Castilla-La Mancha; España
description In the present work on-line derivatization is combined with on-line normal phase liquid chromatography-gas chromatography (NPLC-GC) to analyze total sterols in edible oils. The method uses the TOTAD interface with an additional LC injection valve to automatically introduce the derivatization reagent. The derivatization reaction takes place in the adsorbent material located inside the liner of the TOTAD interface. The samples were saponified with potassium hydroxide in an ethanolic solution and the unsaponifiable fraction was extracted with diethyl ether. The extract was then analyzed by online derivatization with on-line NPLC?GC, avoiding the laborious thin layer chromatography and off-line derivatization steps used in the Official European Union (EU) method. The relative standard deviations (RSDs) from the absolute peak area were lower than 14% except for campestanol. No variability in retention time was observed. Limits of detection (LODs) were less than 8.82 mg/kg. Different edible oils were analyzed using the proposed method and the results obtained were compared with those obtained using the Official EU method. There was good agreement between both methods for all the sterols analyzed.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10731
Toledano, Rosa; Cortés, José; Andini, Juan Carlos; Vázquez, Ana; Villén, Jesús; On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to the analysis of total sterols in edible oils; Elsevier Science; Journal Of Chromatography - A; 1256; 7-2012; 191-196
0021-9673
url http://hdl.handle.net/11336/10731
identifier_str_mv Toledano, Rosa; Cortés, José; Andini, Juan Carlos; Vázquez, Ana; Villén, Jesús; On-line derivatization with on-line coupled normal phase liquid chromatography-gas chromatography using the through oven transfer adsorption desorption interface: Application to the analysis of total sterols in edible oils; Elsevier Science; Journal Of Chromatography - A; 1256; 7-2012; 191-196
0021-9673
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chroma.2012.07.057
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0021967312011120
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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