DNAzymes and ribozymes carrying 2'-C-methyl nucleotides

Autores
Pontiggia, Rodrigo Martin; Robaldo, Laura; Montserrat, Javier Marcelo; Iribarren, Adolfo Marcelo
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
DNAzymes and Ribozymes find applications as inhibitors of gene expression and in detection systems such as biosensors, among others. In particular, we are interested in the properties of hammerhead ribozymes carrying 2′-C-methylnucleotides and 10–23 DNAzymes containing (2′R) or (2′S)- 2′-C-methyl 2′-deoxynucleotides. In this work a new synthesis of 2′-C-Methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies as well as the stability under oligonucleotide synthesis conditions is discussed. 2′-C-methyl-2′-deoxynucleosides show differential preferred conformations depending on the configuration of the 2′-carbon. The influence of these modifications on the activity of 10–23 DNAzymes is also assessed.
Fil: Pontiggia, Rodrigo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Robaldo, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Montserrat, Javier Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
Fil: Iribarren, Adolfo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de Quilmes; Argentina
Materia
Dnazymes
Ribozymes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79645
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79645
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling DNAzymes and ribozymes carrying 2'-C-methyl nucleotidesPontiggia, Rodrigo MartinRobaldo, LauraMontserrat, Javier MarceloIribarren, Adolfo MarceloDnazymesRibozymeshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1DNAzymes and Ribozymes find applications as inhibitors of gene expression and in detection systems such as biosensors, among others. In particular, we are interested in the properties of hammerhead ribozymes carrying 2′-C-methylnucleotides and 10–23 DNAzymes containing (2′R) or (2′S)- 2′-C-methyl 2′-deoxynucleotides. In this work a new synthesis of 2′-C-Methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies as well as the stability under oligonucleotide synthesis conditions is discussed. 2′-C-methyl-2′-deoxynucleosides show differential preferred conformations depending on the configuration of the 2′-carbon. The influence of these modifications on the activity of 10–23 DNAzymes is also assessed.Fil: Pontiggia, Rodrigo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Robaldo, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Montserrat, Javier Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de General Sarmiento. Instituto de Ciencias; ArgentinaFil: Iribarren, Adolfo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de Quilmes; ArgentinaOxford University Press2008-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79645Pontiggia, Rodrigo Martin; Robaldo, Laura; Montserrat, Javier Marcelo; Iribarren, Adolfo Marcelo; DNAzymes and ribozymes carrying 2'-C-methyl nucleotides; Oxford University Press; Nucleic Acids Symposium Series; 52; 1; 9-2008; 521-5220305-10481746-8272CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/nass/article/52/1/521/1108375info:eu-repo/semantics/altIdentifier/doi/10.1093/nass/nrn264info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:24Zoai:ri.conicet.gov.ar:11336/79645instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:24.781CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv DNAzymes and ribozymes carrying 2'-C-methyl nucleotides
title DNAzymes and ribozymes carrying 2'-C-methyl nucleotides
spellingShingle DNAzymes and ribozymes carrying 2'-C-methyl nucleotides
Pontiggia, Rodrigo Martin
Dnazymes
Ribozymes
title_short DNAzymes and ribozymes carrying 2'-C-methyl nucleotides
title_full DNAzymes and ribozymes carrying 2'-C-methyl nucleotides
title_fullStr DNAzymes and ribozymes carrying 2'-C-methyl nucleotides
title_full_unstemmed DNAzymes and ribozymes carrying 2'-C-methyl nucleotides
title_sort DNAzymes and ribozymes carrying 2'-C-methyl nucleotides
dc.creator.none.fl_str_mv Pontiggia, Rodrigo Martin
Robaldo, Laura
Montserrat, Javier Marcelo
Iribarren, Adolfo Marcelo
author Pontiggia, Rodrigo Martin
author_facet Pontiggia, Rodrigo Martin
Robaldo, Laura
Montserrat, Javier Marcelo
Iribarren, Adolfo Marcelo
author_role author
author2 Robaldo, Laura
Montserrat, Javier Marcelo
Iribarren, Adolfo Marcelo
author2_role author
author
author
dc.subject.none.fl_str_mv Dnazymes
Ribozymes
topic Dnazymes
Ribozymes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv DNAzymes and Ribozymes find applications as inhibitors of gene expression and in detection systems such as biosensors, among others. In particular, we are interested in the properties of hammerhead ribozymes carrying 2′-C-methylnucleotides and 10–23 DNAzymes containing (2′R) or (2′S)- 2′-C-methyl 2′-deoxynucleotides. In this work a new synthesis of 2′-C-Methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies as well as the stability under oligonucleotide synthesis conditions is discussed. 2′-C-methyl-2′-deoxynucleosides show differential preferred conformations depending on the configuration of the 2′-carbon. The influence of these modifications on the activity of 10–23 DNAzymes is also assessed.
Fil: Pontiggia, Rodrigo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Robaldo, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Montserrat, Javier Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
Fil: Iribarren, Adolfo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de Quilmes; Argentina
description DNAzymes and Ribozymes find applications as inhibitors of gene expression and in detection systems such as biosensors, among others. In particular, we are interested in the properties of hammerhead ribozymes carrying 2′-C-methylnucleotides and 10–23 DNAzymes containing (2′R) or (2′S)- 2′-C-methyl 2′-deoxynucleotides. In this work a new synthesis of 2′-C-Methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2′-hydroxyl group. Comparison to previous protecting strategies as well as the stability under oligonucleotide synthesis conditions is discussed. 2′-C-methyl-2′-deoxynucleosides show differential preferred conformations depending on the configuration of the 2′-carbon. The influence of these modifications on the activity of 10–23 DNAzymes is also assessed.
publishDate 2008
dc.date.none.fl_str_mv 2008-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79645
Pontiggia, Rodrigo Martin; Robaldo, Laura; Montserrat, Javier Marcelo; Iribarren, Adolfo Marcelo; DNAzymes and ribozymes carrying 2'-C-methyl nucleotides; Oxford University Press; Nucleic Acids Symposium Series; 52; 1; 9-2008; 521-522
0305-1048
1746-8272
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79645
identifier_str_mv Pontiggia, Rodrigo Martin; Robaldo, Laura; Montserrat, Javier Marcelo; Iribarren, Adolfo Marcelo; DNAzymes and ribozymes carrying 2'-C-methyl nucleotides; Oxford University Press; Nucleic Acids Symposium Series; 52; 1; 9-2008; 521-522
0305-1048
1746-8272
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/nass/article/52/1/521/1108375
info:eu-repo/semantics/altIdentifier/doi/10.1093/nass/nrn264
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270002850299904
score 13.13397

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