Hydatid fluid form Echinococcus granulosus is recognized by Clec9A in BMDCs, induces autophagy and polarizes the T cell response towards Th1/Th17 profiles in vitro

Autores
Chop, Maia; Ledo, Camila; Nicolao, María Celeste; Loos, Julia Alexandra; Cumino, Andrea Carina; Rodríguez Rodrígues, Christian Fernando Ariel
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Background: Cystic echinococcosis is caused by Echinococcus granulosus. This parasite develops cysts in the host that are filled with hydatid fluid (HF). Autophagy plays a key role in antigen presentation promoting activation of T cells. The aim of this work is to analyze if HF could polarize the T cell response in vitro. Methods: HF was collected from infected cattle. Expression of Clec9a was evaluated by FACS in FLT3L-BMDCs. Autophagy induction was evaluated by FACS, qPCR, confocal and TEM. Rapamycin (20 nM) and chloroquine (100μM) were used to analyze autophagic flux. T cell cytokine profiles were analyzed by qPCR. Results: HF induces a downmodulation of Clec9a in BMDCs (n=3 **p<0.01). Enhanced number of autophagosomes were observed by TEM in HF-stimulated BMDCs compared to control cells. Also, HF-stimulated BMDCs significantly enhanced the MFI of LC3+ structures in comparison with unstimulated cells (**p<0.01). The autophagic flux was analyzed by measuring LC3II attached to autophagosome membranes by FACS. HF induced an increased in the percentage of LC3+ cells compared to untreated cells (30.2% vs 23.1%) and this phenomenon was enhanced by the inhibition of lysosomal acidification (28.4% vs 40.6%). Then, we evaluated the expression of beclin-1, lc3, tfeb and atg16l-1 genes. We observed that HF induces a significant increase in the transcriptional expression of lc3 and beclin-1 (n=3, **p <0.01 vs control). Finally, we measured the expression of il-4, il-5, il-6, il-10, il-12, il-13, il-17, tnf-, tgf- and inf-in splenocytes by qPCR, finding that HF-stimulated BMDCs increases significantly the levels of il-6, il-10, il-12, tnf-a, tgf-b and inf-g(n = 3, *p<0,05, **p<0,01, vs control). Conclusions: These results suggest that HF could be recognized by Clec9a in BMDCs, inducing autophagy and promoting antigen presentation. HF-stimulated BMDCs modulate gene expression of cytokines related to the Th1, Th17 and Treg profiles, and strongly inhibit Th2 response.
Fil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Ledo, Camila. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Nicolao, María Celeste. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Loos, Julia Alexandra. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Rodríguez Rodrígues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Inmunología
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
HYDATID FLUID
BONE MARROW DENDRITIC CELLS
AUTOPHAGY
T-CELL RESPONSE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/259396
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/259396
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network_name_str CONICET Digital (CONICET)
spelling Hydatid fluid form Echinococcus granulosus is recognized by Clec9A in BMDCs, induces autophagy and polarizes the T cell response towards Th1/Th17 profiles in vitroChop, MaiaLedo, CamilaNicolao, María CelesteLoos, Julia AlexandraCumino, Andrea CarinaRodríguez Rodrígues, Christian Fernando ArielHYDATID FLUIDBONE MARROW DENDRITIC CELLSAUTOPHAGYT-CELL RESPONSEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Cystic echinococcosis is caused by Echinococcus granulosus. This parasite develops cysts in the host that are filled with hydatid fluid (HF). Autophagy plays a key role in antigen presentation promoting activation of T cells. The aim of this work is to analyze if HF could polarize the T cell response in vitro. Methods: HF was collected from infected cattle. Expression of Clec9a was evaluated by FACS in FLT3L-BMDCs. Autophagy induction was evaluated by FACS, qPCR, confocal and TEM. Rapamycin (20 nM) and chloroquine (100μM) were used to analyze autophagic flux. T cell cytokine profiles were analyzed by qPCR. Results: HF induces a downmodulation of Clec9a in BMDCs (n=3 **p<0.01). Enhanced number of autophagosomes were observed by TEM in HF-stimulated BMDCs compared to control cells. Also, HF-stimulated BMDCs significantly enhanced the MFI of LC3+ structures in comparison with unstimulated cells (**p<0.01). The autophagic flux was analyzed by measuring LC3II attached to autophagosome membranes by FACS. HF induced an increased in the percentage of LC3+ cells compared to untreated cells (30.2% vs 23.1%) and this phenomenon was enhanced by the inhibition of lysosomal acidification (28.4% vs 40.6%). Then, we evaluated the expression of beclin-1, lc3, tfeb and atg16l-1 genes. We observed that HF induces a significant increase in the transcriptional expression of lc3 and beclin-1 (n=3, **p <0.01 vs control). Finally, we measured the expression of il-4, il-5, il-6, il-10, il-12, il-13, il-17, tnf-, tgf- and inf-in splenocytes by qPCR, finding that HF-stimulated BMDCs increases significantly the levels of il-6, il-10, il-12, tnf-a, tgf-b and inf-g(n = 3, *p<0,05, **p<0,01, vs control). Conclusions: These results suggest that HF could be recognized by Clec9a in BMDCs, inducing autophagy and promoting antigen presentation. HF-stimulated BMDCs modulate gene expression of cytokines related to the Th1, Th17 and Treg profiles, and strongly inhibit Th2 response.Fil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Ledo, Camila. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Nicolao, María Celeste. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Loos, Julia Alexandra. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; ArgentinaFil: Rodríguez Rodrígues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaLXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de InmunologíaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/259396Hydatid fluid form Echinococcus granulosus is recognized by Clec9A in BMDCs, induces autophagy and polarizes the T cell response towards Th1/Th17 profiles in vitro; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 148-1481669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol82-22/s5/1s5.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:02Zoai:ri.conicet.gov.ar:11336/259396instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:02.424CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hydatid fluid form Echinococcus granulosus is recognized by Clec9A in BMDCs, induces autophagy and polarizes the T cell response towards Th1/Th17 profiles in vitro
title Hydatid fluid form Echinococcus granulosus is recognized by Clec9A in BMDCs, induces autophagy and polarizes the T cell response towards Th1/Th17 profiles in vitro
spellingShingle Hydatid fluid form Echinococcus granulosus is recognized by Clec9A in BMDCs, induces autophagy and polarizes the T cell response towards Th1/Th17 profiles in vitro
Chop, Maia
HYDATID FLUID
BONE MARROW DENDRITIC CELLS
AUTOPHAGY
T-CELL RESPONSE
title_short Hydatid fluid form Echinococcus granulosus is recognized by Clec9A in BMDCs, induces autophagy and polarizes the T cell response towards Th1/Th17 profiles in vitro
title_full Hydatid fluid form Echinococcus granulosus is recognized by Clec9A in BMDCs, induces autophagy and polarizes the T cell response towards Th1/Th17 profiles in vitro
title_fullStr Hydatid fluid form Echinococcus granulosus is recognized by Clec9A in BMDCs, induces autophagy and polarizes the T cell response towards Th1/Th17 profiles in vitro
title_full_unstemmed Hydatid fluid form Echinococcus granulosus is recognized by Clec9A in BMDCs, induces autophagy and polarizes the T cell response towards Th1/Th17 profiles in vitro
title_sort Hydatid fluid form Echinococcus granulosus is recognized by Clec9A in BMDCs, induces autophagy and polarizes the T cell response towards Th1/Th17 profiles in vitro
dc.creator.none.fl_str_mv Chop, Maia
Ledo, Camila
Nicolao, María Celeste
Loos, Julia Alexandra
Cumino, Andrea Carina
Rodríguez Rodrígues, Christian Fernando Ariel
author Chop, Maia
author_facet Chop, Maia
Ledo, Camila
Nicolao, María Celeste
Loos, Julia Alexandra
Cumino, Andrea Carina
Rodríguez Rodrígues, Christian Fernando Ariel
author_role author
author2 Ledo, Camila
Nicolao, María Celeste
Loos, Julia Alexandra
Cumino, Andrea Carina
Rodríguez Rodrígues, Christian Fernando Ariel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv HYDATID FLUID
BONE MARROW DENDRITIC CELLS
AUTOPHAGY
T-CELL RESPONSE
topic HYDATID FLUID
BONE MARROW DENDRITIC CELLS
AUTOPHAGY
T-CELL RESPONSE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Cystic echinococcosis is caused by Echinococcus granulosus. This parasite develops cysts in the host that are filled with hydatid fluid (HF). Autophagy plays a key role in antigen presentation promoting activation of T cells. The aim of this work is to analyze if HF could polarize the T cell response in vitro. Methods: HF was collected from infected cattle. Expression of Clec9a was evaluated by FACS in FLT3L-BMDCs. Autophagy induction was evaluated by FACS, qPCR, confocal and TEM. Rapamycin (20 nM) and chloroquine (100μM) were used to analyze autophagic flux. T cell cytokine profiles were analyzed by qPCR. Results: HF induces a downmodulation of Clec9a in BMDCs (n=3 **p<0.01). Enhanced number of autophagosomes were observed by TEM in HF-stimulated BMDCs compared to control cells. Also, HF-stimulated BMDCs significantly enhanced the MFI of LC3+ structures in comparison with unstimulated cells (**p<0.01). The autophagic flux was analyzed by measuring LC3II attached to autophagosome membranes by FACS. HF induced an increased in the percentage of LC3+ cells compared to untreated cells (30.2% vs 23.1%) and this phenomenon was enhanced by the inhibition of lysosomal acidification (28.4% vs 40.6%). Then, we evaluated the expression of beclin-1, lc3, tfeb and atg16l-1 genes. We observed that HF induces a significant increase in the transcriptional expression of lc3 and beclin-1 (n=3, **p <0.01 vs control). Finally, we measured the expression of il-4, il-5, il-6, il-10, il-12, il-13, il-17, tnf-, tgf- and inf-in splenocytes by qPCR, finding that HF-stimulated BMDCs increases significantly the levels of il-6, il-10, il-12, tnf-a, tgf-b and inf-g(n = 3, *p<0,05, **p<0,01, vs control). Conclusions: These results suggest that HF could be recognized by Clec9a in BMDCs, inducing autophagy and promoting antigen presentation. HF-stimulated BMDCs modulate gene expression of cytokines related to the Th1, Th17 and Treg profiles, and strongly inhibit Th2 response.
Fil: Chop, Maia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Ledo, Camila. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Nicolao, María Celeste. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Loos, Julia Alexandra. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Cumino, Andrea Carina. Universidad Nacional de Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Investigaciones En Produccion, Sanidad y Ambiente.; Argentina
Fil: Rodríguez Rodrígues, Christian Fernando Ariel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Inmunología
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description Background: Cystic echinococcosis is caused by Echinococcus granulosus. This parasite develops cysts in the host that are filled with hydatid fluid (HF). Autophagy plays a key role in antigen presentation promoting activation of T cells. The aim of this work is to analyze if HF could polarize the T cell response in vitro. Methods: HF was collected from infected cattle. Expression of Clec9a was evaluated by FACS in FLT3L-BMDCs. Autophagy induction was evaluated by FACS, qPCR, confocal and TEM. Rapamycin (20 nM) and chloroquine (100μM) were used to analyze autophagic flux. T cell cytokine profiles were analyzed by qPCR. Results: HF induces a downmodulation of Clec9a in BMDCs (n=3 **p<0.01). Enhanced number of autophagosomes were observed by TEM in HF-stimulated BMDCs compared to control cells. Also, HF-stimulated BMDCs significantly enhanced the MFI of LC3+ structures in comparison with unstimulated cells (**p<0.01). The autophagic flux was analyzed by measuring LC3II attached to autophagosome membranes by FACS. HF induced an increased in the percentage of LC3+ cells compared to untreated cells (30.2% vs 23.1%) and this phenomenon was enhanced by the inhibition of lysosomal acidification (28.4% vs 40.6%). Then, we evaluated the expression of beclin-1, lc3, tfeb and atg16l-1 genes. We observed that HF induces a significant increase in the transcriptional expression of lc3 and beclin-1 (n=3, **p <0.01 vs control). Finally, we measured the expression of il-4, il-5, il-6, il-10, il-12, il-13, il-17, tnf-, tgf- and inf-in splenocytes by qPCR, finding that HF-stimulated BMDCs increases significantly the levels of il-6, il-10, il-12, tnf-a, tgf-b and inf-g(n = 3, *p<0,05, **p<0,01, vs control). Conclusions: These results suggest that HF could be recognized by Clec9a in BMDCs, inducing autophagy and promoting antigen presentation. HF-stimulated BMDCs modulate gene expression of cytokines related to the Th1, Th17 and Treg profiles, and strongly inhibit Th2 response.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/259396
Hydatid fluid form Echinococcus granulosus is recognized by Clec9A in BMDCs, induces autophagy and polarizes the T cell response towards Th1/Th17 profiles in vitro; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 148-148
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/259396
identifier_str_mv Hydatid fluid form Echinococcus granulosus is recognized by Clec9A in BMDCs, induces autophagy and polarizes the T cell response towards Th1/Th17 profiles in vitro; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 148-148
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol82-22/s5/1s5.pdf
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application/pdf
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application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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