Characterization of genetic diversity of Trachemys dorbigni and Phrynops hilarii

Autores
Guidetti, Brenda Yamile; Siroski, Pablo Ariel; Amavet, Patricia Susana
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The utilization of RAPD and ISSR molecular markers is proposed to initiate studies of genetic variability in Phrynops hilarii and Trachemys dorbigni, two species of fresh water turtles distributed in South America. Three primers of RAPD and four of ISSR were selected and the amplified products of these markers were evaluated by electrophoretic runs in agarose and polyacrylamide gels. The levels of heterozygosity, Shannon index and different allele numbers were slightly higher in P. hilarii for both types of markers. Levels of polymorphism were also higher in P. hilarii than T. dorbigni and both were elevated compared to those recorded for other species. The fact that similar results were obtained with both types of markers for all estimates of diversity highlights the usefulness and validity of the RAPD technique. The molecular markers used were found potentially useful for analysing future temporal and spatial distribution of genetic diversity in both species, expanding scales work.
Fil: Guidetti, Brenda Yamile. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Siroski, Pablo Ariel. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Amavet, Patricia Susana. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
Fresh Water Turtles
Variability
Molecular Markers
Rapd
Issr
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16795
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of genetic diversity of Trachemys dorbigni and Phrynops hilariiGuidetti, Brenda YamileSiroski, Pablo ArielAmavet, Patricia SusanaFresh Water TurtlesVariabilityMolecular MarkersRapdIssrhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The utilization of RAPD and ISSR molecular markers is proposed to initiate studies of genetic variability in Phrynops hilarii and Trachemys dorbigni, two species of fresh water turtles distributed in South America. Three primers of RAPD and four of ISSR were selected and the amplified products of these markers were evaluated by electrophoretic runs in agarose and polyacrylamide gels. The levels of heterozygosity, Shannon index and different allele numbers were slightly higher in P. hilarii for both types of markers. Levels of polymorphism were also higher in P. hilarii than T. dorbigni and both were elevated compared to those recorded for other species. The fact that similar results were obtained with both types of markers for all estimates of diversity highlights the usefulness and validity of the RAPD technique. The molecular markers used were found potentially useful for analysing future temporal and spatial distribution of genetic diversity in both species, expanding scales work.Fil: Guidetti, Brenda Yamile. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Siroski, Pablo Ariel. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Amavet, Patricia Susana. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaScience Press2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16795Guidetti, Brenda Yamile; Siroski, Pablo Ariel; Amavet, Patricia Susana; Characterization of genetic diversity of Trachemys dorbigni and Phrynops hilarii; Science Press; Asian Herpetological Research; 6; 3; 7-2015; 213-2192095-0357enginfo:eu-repo/semantics/altIdentifier/doi/10.16373/j.cnki.ahr.140055info:eu-repo/semantics/altIdentifier/url/http://www.cnki.net/kcms/doi/10.16373/j.cnki.ahr.140055.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:19Zoai:ri.conicet.gov.ar:11336/16795instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:19.899CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of genetic diversity of Trachemys dorbigni and Phrynops hilarii
title Characterization of genetic diversity of Trachemys dorbigni and Phrynops hilarii
spellingShingle Characterization of genetic diversity of Trachemys dorbigni and Phrynops hilarii
Guidetti, Brenda Yamile
Fresh Water Turtles
Variability
Molecular Markers
Rapd
Issr
title_short Characterization of genetic diversity of Trachemys dorbigni and Phrynops hilarii
title_full Characterization of genetic diversity of Trachemys dorbigni and Phrynops hilarii
title_fullStr Characterization of genetic diversity of Trachemys dorbigni and Phrynops hilarii
title_full_unstemmed Characterization of genetic diversity of Trachemys dorbigni and Phrynops hilarii
title_sort Characterization of genetic diversity of Trachemys dorbigni and Phrynops hilarii
dc.creator.none.fl_str_mv Guidetti, Brenda Yamile
Siroski, Pablo Ariel
Amavet, Patricia Susana
author Guidetti, Brenda Yamile
author_facet Guidetti, Brenda Yamile
Siroski, Pablo Ariel
Amavet, Patricia Susana
author_role author
author2 Siroski, Pablo Ariel
Amavet, Patricia Susana
author2_role author
author
dc.subject.none.fl_str_mv Fresh Water Turtles
Variability
Molecular Markers
Rapd
Issr
topic Fresh Water Turtles
Variability
Molecular Markers
Rapd
Issr
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The utilization of RAPD and ISSR molecular markers is proposed to initiate studies of genetic variability in Phrynops hilarii and Trachemys dorbigni, two species of fresh water turtles distributed in South America. Three primers of RAPD and four of ISSR were selected and the amplified products of these markers were evaluated by electrophoretic runs in agarose and polyacrylamide gels. The levels of heterozygosity, Shannon index and different allele numbers were slightly higher in P. hilarii for both types of markers. Levels of polymorphism were also higher in P. hilarii than T. dorbigni and both were elevated compared to those recorded for other species. The fact that similar results were obtained with both types of markers for all estimates of diversity highlights the usefulness and validity of the RAPD technique. The molecular markers used were found potentially useful for analysing future temporal and spatial distribution of genetic diversity in both species, expanding scales work.
Fil: Guidetti, Brenda Yamile. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Siroski, Pablo Ariel. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Cs.naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (fhuc-unl/maspyma); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Amavet, Patricia Susana. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description The utilization of RAPD and ISSR molecular markers is proposed to initiate studies of genetic variability in Phrynops hilarii and Trachemys dorbigni, two species of fresh water turtles distributed in South America. Three primers of RAPD and four of ISSR were selected and the amplified products of these markers were evaluated by electrophoretic runs in agarose and polyacrylamide gels. The levels of heterozygosity, Shannon index and different allele numbers were slightly higher in P. hilarii for both types of markers. Levels of polymorphism were also higher in P. hilarii than T. dorbigni and both were elevated compared to those recorded for other species. The fact that similar results were obtained with both types of markers for all estimates of diversity highlights the usefulness and validity of the RAPD technique. The molecular markers used were found potentially useful for analysing future temporal and spatial distribution of genetic diversity in both species, expanding scales work.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16795
Guidetti, Brenda Yamile; Siroski, Pablo Ariel; Amavet, Patricia Susana; Characterization of genetic diversity of Trachemys dorbigni and Phrynops hilarii; Science Press; Asian Herpetological Research; 6; 3; 7-2015; 213-219
2095-0357
url http://hdl.handle.net/11336/16795
identifier_str_mv Guidetti, Brenda Yamile; Siroski, Pablo Ariel; Amavet, Patricia Susana; Characterization of genetic diversity of Trachemys dorbigni and Phrynops hilarii; Science Press; Asian Herpetological Research; 6; 3; 7-2015; 213-219
2095-0357
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.16373/j.cnki.ahr.140055
info:eu-repo/semantics/altIdentifier/url/http://www.cnki.net/kcms/doi/10.16373/j.cnki.ahr.140055.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Science Press
publisher.none.fl_str_mv Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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