Assessment of induced genetic variability through physical mutations in Cenchrus ciliaris L. By Issr markers
- Autores
- López Colomba, Eliana; Carrizo, María; Ribotta, Andrea; Quiroga, Mariana; Carloni, Edgardo; Griffa, Sabrina; Tommasino, Exequiel; Grunberg, Karina
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Fil: López Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Fil: Carrizo, María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Fil: Ribotta, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Fil: Quiroga, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Fil: Carloni, Edgardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Fil: Griffa, Sabrina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Fil: Tommasino, Exequiel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Cenchrus ciliaris L., is an important gramineous used as forage for ruminants in north-west areas of Argentina. It reproduces mainly by aposporous apomixis. One way of obtaining a novel germplasm is by using induced physical mutations as X rays. Because of the random nature of mutagenic agents, it is necessary to use accurate techniques that enable to characterize the new genotypes. In the last decade, the inter-simple sequence repeat (ISSR) markers have been recognized as low-cost and useful molecular markers for genetic variability analysis. The aims of this work were to estimate the induced genetic variability among putative mutants by means ISSR markers and evaluate the discrimination capacity of this technique. For induce mutations, mature seeds of RN 49, RN 51 and RN 148 accessions were exposed to X rays (400 Gy). Following mutagenic treatment, fresh leaves of obtained seedlings were used to perform DNA extraction. A total of 28 primers were screened initially. The 19 putative mutants obtained and the three respective controls generated highly reproducible patterns, with multiple discrete bands when they were amplified with the three most polymorphic ISSR primers. Of the 35 amplified bands, 30 corresponded to polymorphic fragments (85.71%) detected with the primers UBC840A, UBC840B and UBC816. All evaluated plants were different respect to respective controls. The present work is the first report of molecular characterization through ISSR markers of Cenchrus ciliaris mutants. This technique revealed a high degree of polymorphism and was successfully used to differentiate these materials. The results suggest that induced mutations may be used as a novel approach within the framework of a genetic improvement program to increase genetic diversity in Cenchrus ciliaris. Further analysis will be necessary to evaluate the suitability of these new genetic resources in terms of biomass production and quality to be incorporated to cattle raising systems.
Fil: López Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Fil: Carrizo, María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Fil: Ribotta, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Fil: Quiroga, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Fil: Carloni, Edgardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Fil: Griffa, Sabrina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Fil: Tommasino, Exequiel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.
Otras Ciencias Agrícolas - Materia
-
Genetic variability
Physical mutations
Cenchrus ciliaris
Issr markers - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Universidad Nacional de Córdoba
- OAI Identificador
- oai:rdu.unc.edu.ar:11086/558471
Ver los metadatos del registro completo
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Assessment of induced genetic variability through physical mutations in Cenchrus ciliaris L. By Issr markersLópez Colomba, ElianaCarrizo, MaríaRibotta, AndreaQuiroga, MarianaCarloni, EdgardoGriffa, SabrinaTommasino, ExequielGrunberg, KarinaGenetic variabilityPhysical mutationsCenchrus ciliarisIssr markersFil: López Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Fil: Carrizo, María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Fil: Ribotta, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Fil: Quiroga, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Fil: Carloni, Edgardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Fil: Griffa, Sabrina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Fil: Tommasino, Exequiel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Cenchrus ciliaris L., is an important gramineous used as forage for ruminants in north-west areas of Argentina. It reproduces mainly by aposporous apomixis. One way of obtaining a novel germplasm is by using induced physical mutations as X rays. Because of the random nature of mutagenic agents, it is necessary to use accurate techniques that enable to characterize the new genotypes. In the last decade, the inter-simple sequence repeat (ISSR) markers have been recognized as low-cost and useful molecular markers for genetic variability analysis. The aims of this work were to estimate the induced genetic variability among putative mutants by means ISSR markers and evaluate the discrimination capacity of this technique. For induce mutations, mature seeds of RN 49, RN 51 and RN 148 accessions were exposed to X rays (400 Gy). Following mutagenic treatment, fresh leaves of obtained seedlings were used to perform DNA extraction. A total of 28 primers were screened initially. The 19 putative mutants obtained and the three respective controls generated highly reproducible patterns, with multiple discrete bands when they were amplified with the three most polymorphic ISSR primers. Of the 35 amplified bands, 30 corresponded to polymorphic fragments (85.71%) detected with the primers UBC840A, UBC840B and UBC816. All evaluated plants were different respect to respective controls. The present work is the first report of molecular characterization through ISSR markers of Cenchrus ciliaris mutants. This technique revealed a high degree of polymorphism and was successfully used to differentiate these materials. The results suggest that induced mutations may be used as a novel approach within the framework of a genetic improvement program to increase genetic diversity in Cenchrus ciliaris. Further analysis will be necessary to evaluate the suitability of these new genetic resources in terms of biomass production and quality to be incorporated to cattle raising systems.Fil: López Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Fil: Carrizo, María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Fil: Ribotta, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Fil: Quiroga, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Fil: Carloni, Edgardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Fil: Griffa, Sabrina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Fil: Tommasino, Exequiel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina.Otras Ciencias Agrícolas2015info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/11086/558471enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-10-16T09:28:05Zoai:rdu.unc.edu.ar:11086/558471Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-10-16 09:28:05.741Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse |
| dc.title.none.fl_str_mv |
Assessment of induced genetic variability through physical mutations in Cenchrus ciliaris L. By Issr markers |
| title |
Assessment of induced genetic variability through physical mutations in Cenchrus ciliaris L. By Issr markers |
| spellingShingle |
Assessment of induced genetic variability through physical mutations in Cenchrus ciliaris L. By Issr markers López Colomba, Eliana Genetic variability Physical mutations Cenchrus ciliaris Issr markers |
| title_short |
Assessment of induced genetic variability through physical mutations in Cenchrus ciliaris L. By Issr markers |
| title_full |
Assessment of induced genetic variability through physical mutations in Cenchrus ciliaris L. By Issr markers |
| title_fullStr |
Assessment of induced genetic variability through physical mutations in Cenchrus ciliaris L. By Issr markers |
| title_full_unstemmed |
Assessment of induced genetic variability through physical mutations in Cenchrus ciliaris L. By Issr markers |
| title_sort |
Assessment of induced genetic variability through physical mutations in Cenchrus ciliaris L. By Issr markers |
| dc.creator.none.fl_str_mv |
López Colomba, Eliana Carrizo, María Ribotta, Andrea Quiroga, Mariana Carloni, Edgardo Griffa, Sabrina Tommasino, Exequiel Grunberg, Karina |
| author |
López Colomba, Eliana |
| author_facet |
López Colomba, Eliana Carrizo, María Ribotta, Andrea Quiroga, Mariana Carloni, Edgardo Griffa, Sabrina Tommasino, Exequiel Grunberg, Karina |
| author_role |
author |
| author2 |
Carrizo, María Ribotta, Andrea Quiroga, Mariana Carloni, Edgardo Griffa, Sabrina Tommasino, Exequiel Grunberg, Karina |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
Genetic variability Physical mutations Cenchrus ciliaris Issr markers |
| topic |
Genetic variability Physical mutations Cenchrus ciliaris Issr markers |
| dc.description.none.fl_txt_mv |
Fil: López Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Fil: Carrizo, María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Fil: Ribotta, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Fil: Quiroga, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Fil: Carloni, Edgardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Fil: Griffa, Sabrina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Fil: Tommasino, Exequiel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Cenchrus ciliaris L., is an important gramineous used as forage for ruminants in north-west areas of Argentina. It reproduces mainly by aposporous apomixis. One way of obtaining a novel germplasm is by using induced physical mutations as X rays. Because of the random nature of mutagenic agents, it is necessary to use accurate techniques that enable to characterize the new genotypes. In the last decade, the inter-simple sequence repeat (ISSR) markers have been recognized as low-cost and useful molecular markers for genetic variability analysis. The aims of this work were to estimate the induced genetic variability among putative mutants by means ISSR markers and evaluate the discrimination capacity of this technique. For induce mutations, mature seeds of RN 49, RN 51 and RN 148 accessions were exposed to X rays (400 Gy). Following mutagenic treatment, fresh leaves of obtained seedlings were used to perform DNA extraction. A total of 28 primers were screened initially. The 19 putative mutants obtained and the three respective controls generated highly reproducible patterns, with multiple discrete bands when they were amplified with the three most polymorphic ISSR primers. Of the 35 amplified bands, 30 corresponded to polymorphic fragments (85.71%) detected with the primers UBC840A, UBC840B and UBC816. All evaluated plants were different respect to respective controls. The present work is the first report of molecular characterization through ISSR markers of Cenchrus ciliaris mutants. This technique revealed a high degree of polymorphism and was successfully used to differentiate these materials. The results suggest that induced mutations may be used as a novel approach within the framework of a genetic improvement program to increase genetic diversity in Cenchrus ciliaris. Further analysis will be necessary to evaluate the suitability of these new genetic resources in terms of biomass production and quality to be incorporated to cattle raising systems. Fil: López Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Fil: Carrizo, María. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Fil: Ribotta, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Fil: Quiroga, Mariana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Fil: Carloni, Edgardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Fil: Griffa, Sabrina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Fil: Tommasino, Exequiel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. Otras Ciencias Agrícolas |
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Fil: López Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias; Argentina. |
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2015 |
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2015 |
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