Kinetics of corneal leukocytes by intravital multiphoton microscopy

Autores
Seyed-Razavi, Yashar; Lopez, Maria J.; Mantopoulos, Dimosthenis; Zheng, Lixin; Massberg, Steffen; Sendra, Victor German; Harris, Deshea L.; Hamrah, Pedram
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Corneal immune privilege is integral in maintaining the clear avascular window to the foreign world. The presence of distinct populations of corneal leukocytes (CLs) in the normal cornea has been firmly established. However, their precise function and kinetics remain, as of yet, unclear. Through intravital multiphoton microscopy (IV-MPM), allowing themeans to accumulate critical spatial and temporal cellular information, we provide details for long-term investigation of CL morphology and kinetics under steady state and following inflammation. Significant alterations in size and morphology of corneal CD11c+ dendritic cells (DCs) were noted following acute sterile inflammation, including cellvolume(4364.4±489.6 vs. 1787.6±111.0mm3,P<0.001)andsphericity (0.82±0.01 vs. 0.42± 0.02,P<0.001) comparedwith steady state. Furthermore, IV-MPManalyses revealed alterations in both theCD11c+DC and major histocompatibility complex class II (MHC)-II+ mature antigen-presenting cell population kinetics during inflammation, including track displacement length (CD11c: 16.57±1.41 vs. 4.64±0.56 μm, P < 0.001;MHC-II: 9.03± 0.37 vs. 4.09±0.39,P<0.001) andvelocity (CD11c: 1.91±0.07μm/minvs. 1.73±0.1302μm/min; MHC-II: 2.97±0.07 vs. 1.62 ± 0.08, P < 0.001) compared with steady state. Our results reveal in vivo evidence of sessile CL populations exhibiting dendritic morphology under steady state and increased velocity of spherical leukocytes following inflammation. IV-MPMrepresents a powerful tool to study leukocytes in corneal diseases in context.
Fil: Seyed-Razavi, Yashar. Tufts University; Estados Unidos
Fil: Lopez, Maria J.. Tufts University; Estados Unidos
Fil: Mantopoulos, Dimosthenis. Harvard Medical School; Estados Unidos
Fil: Zheng, Lixin. Harvard Medical School; Estados Unidos
Fil: Massberg, Steffen. Ludwig Maximilians Universitat; Alemania. Harvard Medical School; Estados Unidos
Fil: Sendra, Victor German. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología; Argentina. Tufts University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Harris, Deshea L.. Tufts University; Estados Unidos
Fil: Hamrah, Pedram. Harvard Medical School; Estados Unidos
Materia
ANTIGEN-PRESENTING CELLS
CELL MORPHOLOGY
DENDRITIC CELLS
KERATITIS
MHC-II
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/124739
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/124739
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Kinetics of corneal leukocytes by intravital multiphoton microscopySeyed-Razavi, YasharLopez, Maria J.Mantopoulos, DimosthenisZheng, LixinMassberg, SteffenSendra, Victor GermanHarris, Deshea L.Hamrah, PedramANTIGEN-PRESENTING CELLSCELL MORPHOLOGYDENDRITIC CELLSKERATITISMHC-IIhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Corneal immune privilege is integral in maintaining the clear avascular window to the foreign world. The presence of distinct populations of corneal leukocytes (CLs) in the normal cornea has been firmly established. However, their precise function and kinetics remain, as of yet, unclear. Through intravital multiphoton microscopy (IV-MPM), allowing themeans to accumulate critical spatial and temporal cellular information, we provide details for long-term investigation of CL morphology and kinetics under steady state and following inflammation. Significant alterations in size and morphology of corneal CD11c+ dendritic cells (DCs) were noted following acute sterile inflammation, including cellvolume(4364.4±489.6 vs. 1787.6±111.0mm3,P<0.001)andsphericity (0.82±0.01 vs. 0.42± 0.02,P<0.001) comparedwith steady state. Furthermore, IV-MPManalyses revealed alterations in both theCD11c+DC and major histocompatibility complex class II (MHC)-II+ mature antigen-presenting cell population kinetics during inflammation, including track displacement length (CD11c: 16.57±1.41 vs. 4.64±0.56 μm, P < 0.001;MHC-II: 9.03± 0.37 vs. 4.09±0.39,P<0.001) andvelocity (CD11c: 1.91±0.07μm/minvs. 1.73±0.1302μm/min; MHC-II: 2.97±0.07 vs. 1.62 ± 0.08, P < 0.001) compared with steady state. Our results reveal in vivo evidence of sessile CL populations exhibiting dendritic morphology under steady state and increased velocity of spherical leukocytes following inflammation. IV-MPMrepresents a powerful tool to study leukocytes in corneal diseases in context.Fil: Seyed-Razavi, Yashar. Tufts University; Estados UnidosFil: Lopez, Maria J.. Tufts University; Estados UnidosFil: Mantopoulos, Dimosthenis. Harvard Medical School; Estados UnidosFil: Zheng, Lixin. Harvard Medical School; Estados UnidosFil: Massberg, Steffen. Ludwig Maximilians Universitat; Alemania. Harvard Medical School; Estados UnidosFil: Sendra, Victor German. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología; Argentina. Tufts University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Harris, Deshea L.. Tufts University; Estados UnidosFil: Hamrah, Pedram. Harvard Medical School; Estados UnidosFederation of American Societies for Experimental Biology2019-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/124739Seyed-Razavi, Yashar; Lopez, Maria J.; Mantopoulos, Dimosthenis; Zheng, Lixin; Massberg, Steffen; et al.; Kinetics of corneal leukocytes by intravital multiphoton microscopy; Federation of American Societies for Experimental Biology; FASEB Journal; 33; 2; 2-2019; 2199-22110892-66381530-6860CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/30226811info:eu-repo/semantics/altIdentifier/doi/10.1096/fj.201800684RRinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:45:23Zoai:ri.conicet.gov.ar:11336/124739instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:45:23.243CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Kinetics of corneal leukocytes by intravital multiphoton microscopy
title Kinetics of corneal leukocytes by intravital multiphoton microscopy
spellingShingle Kinetics of corneal leukocytes by intravital multiphoton microscopy
Seyed-Razavi, Yashar
ANTIGEN-PRESENTING CELLS
CELL MORPHOLOGY
DENDRITIC CELLS
KERATITIS
MHC-II
title_short Kinetics of corneal leukocytes by intravital multiphoton microscopy
title_full Kinetics of corneal leukocytes by intravital multiphoton microscopy
title_fullStr Kinetics of corneal leukocytes by intravital multiphoton microscopy
title_full_unstemmed Kinetics of corneal leukocytes by intravital multiphoton microscopy
title_sort Kinetics of corneal leukocytes by intravital multiphoton microscopy
dc.creator.none.fl_str_mv Seyed-Razavi, Yashar
Lopez, Maria J.
Mantopoulos, Dimosthenis
Zheng, Lixin
Massberg, Steffen
Sendra, Victor German
Harris, Deshea L.
Hamrah, Pedram
author Seyed-Razavi, Yashar
author_facet Seyed-Razavi, Yashar
Lopez, Maria J.
Mantopoulos, Dimosthenis
Zheng, Lixin
Massberg, Steffen
Sendra, Victor German
Harris, Deshea L.
Hamrah, Pedram
author_role author
author2 Lopez, Maria J.
Mantopoulos, Dimosthenis
Zheng, Lixin
Massberg, Steffen
Sendra, Victor German
Harris, Deshea L.
Hamrah, Pedram
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIGEN-PRESENTING CELLS
CELL MORPHOLOGY
DENDRITIC CELLS
KERATITIS
MHC-II
topic ANTIGEN-PRESENTING CELLS
CELL MORPHOLOGY
DENDRITIC CELLS
KERATITIS
MHC-II
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Corneal immune privilege is integral in maintaining the clear avascular window to the foreign world. The presence of distinct populations of corneal leukocytes (CLs) in the normal cornea has been firmly established. However, their precise function and kinetics remain, as of yet, unclear. Through intravital multiphoton microscopy (IV-MPM), allowing themeans to accumulate critical spatial and temporal cellular information, we provide details for long-term investigation of CL morphology and kinetics under steady state and following inflammation. Significant alterations in size and morphology of corneal CD11c+ dendritic cells (DCs) were noted following acute sterile inflammation, including cellvolume(4364.4±489.6 vs. 1787.6±111.0mm3,P<0.001)andsphericity (0.82±0.01 vs. 0.42± 0.02,P<0.001) comparedwith steady state. Furthermore, IV-MPManalyses revealed alterations in both theCD11c+DC and major histocompatibility complex class II (MHC)-II+ mature antigen-presenting cell population kinetics during inflammation, including track displacement length (CD11c: 16.57±1.41 vs. 4.64±0.56 μm, P < 0.001;MHC-II: 9.03± 0.37 vs. 4.09±0.39,P<0.001) andvelocity (CD11c: 1.91±0.07μm/minvs. 1.73±0.1302μm/min; MHC-II: 2.97±0.07 vs. 1.62 ± 0.08, P < 0.001) compared with steady state. Our results reveal in vivo evidence of sessile CL populations exhibiting dendritic morphology under steady state and increased velocity of spherical leukocytes following inflammation. IV-MPMrepresents a powerful tool to study leukocytes in corneal diseases in context.
Fil: Seyed-Razavi, Yashar. Tufts University; Estados Unidos
Fil: Lopez, Maria J.. Tufts University; Estados Unidos
Fil: Mantopoulos, Dimosthenis. Harvard Medical School; Estados Unidos
Fil: Zheng, Lixin. Harvard Medical School; Estados Unidos
Fil: Massberg, Steffen. Ludwig Maximilians Universitat; Alemania. Harvard Medical School; Estados Unidos
Fil: Sendra, Victor German. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología; Argentina. Tufts University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Harris, Deshea L.. Tufts University; Estados Unidos
Fil: Hamrah, Pedram. Harvard Medical School; Estados Unidos
description Corneal immune privilege is integral in maintaining the clear avascular window to the foreign world. The presence of distinct populations of corneal leukocytes (CLs) in the normal cornea has been firmly established. However, their precise function and kinetics remain, as of yet, unclear. Through intravital multiphoton microscopy (IV-MPM), allowing themeans to accumulate critical spatial and temporal cellular information, we provide details for long-term investigation of CL morphology and kinetics under steady state and following inflammation. Significant alterations in size and morphology of corneal CD11c+ dendritic cells (DCs) were noted following acute sterile inflammation, including cellvolume(4364.4±489.6 vs. 1787.6±111.0mm3,P<0.001)andsphericity (0.82±0.01 vs. 0.42± 0.02,P<0.001) comparedwith steady state. Furthermore, IV-MPManalyses revealed alterations in both theCD11c+DC and major histocompatibility complex class II (MHC)-II+ mature antigen-presenting cell population kinetics during inflammation, including track displacement length (CD11c: 16.57±1.41 vs. 4.64±0.56 μm, P < 0.001;MHC-II: 9.03± 0.37 vs. 4.09±0.39,P<0.001) andvelocity (CD11c: 1.91±0.07μm/minvs. 1.73±0.1302μm/min; MHC-II: 2.97±0.07 vs. 1.62 ± 0.08, P < 0.001) compared with steady state. Our results reveal in vivo evidence of sessile CL populations exhibiting dendritic morphology under steady state and increased velocity of spherical leukocytes following inflammation. IV-MPMrepresents a powerful tool to study leukocytes in corneal diseases in context.
publishDate 2019
dc.date.none.fl_str_mv 2019-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/124739
Seyed-Razavi, Yashar; Lopez, Maria J.; Mantopoulos, Dimosthenis; Zheng, Lixin; Massberg, Steffen; et al.; Kinetics of corneal leukocytes by intravital multiphoton microscopy; Federation of American Societies for Experimental Biology; FASEB Journal; 33; 2; 2-2019; 2199-2211
0892-6638
1530-6860
CONICET Digital
CONICET
url http://hdl.handle.net/11336/124739
identifier_str_mv Seyed-Razavi, Yashar; Lopez, Maria J.; Mantopoulos, Dimosthenis; Zheng, Lixin; Massberg, Steffen; et al.; Kinetics of corneal leukocytes by intravital multiphoton microscopy; Federation of American Societies for Experimental Biology; FASEB Journal; 33; 2; 2-2019; 2199-2211
0892-6638
1530-6860
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/30226811
info:eu-repo/semantics/altIdentifier/doi/10.1096/fj.201800684RR
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Federation of American Societies for Experimental Biology
publisher.none.fl_str_mv Federation of American Societies for Experimental Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083553832468480
score 13.22299

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