The Mitochondrial Interplay of Ubiquinol and Nitric Oxide in Endotoxemia

Autores
Lisdero, Constanza L.; Carreras, Maria Cecilia; Meulemans, Alain; Melani, Mariana; Aubier, Michel; Boczkowski, Jorge; Poderoso, Juan José
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This chapter focuses on role of ubiquinol (UQ) and nitric oxide in endotoxemia. UQ is endogenously synthesized in every organ in a specific pathway branched from cholesterol biosynthesis. Organs have different ubiquinol content depending on the activity of the biosynthetic pathway. In addition to its role as electron carrier in the mitochondrial respiratory chain, UQ exhibits prooxidant and antioxidant properties. The chapter provides the methodological bases that support the relation between UQ content, mitochondrial NO steady-state concentration, and peroxynitrite-mediated mitochondrial damage in representative organs. The chapter also focuses on the contribution of mtNOS to peroxynitrite formation in mitochondria. The chapter presents an experimental model, elaborating endotoxemic animals and sample preparation, and homogenate and mitochondrial preparations. Sources of NO in endotoxemia are also analyzed in the chapter. The chapter discusses the measurement of NOS expression and activity and analyzes the role of ubiquinol in endotoxemia. The endotoxemic mitochondrial damage is also analyzed in the chapter. The chapter elaborates the ubiquinone content and NO-induced hydrogen peroxide production by mitochondria..
Fil: Lisdero, Constanza L.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Carreras, Maria Cecilia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Meulemans, Alain. Universite de Paris; Francia
Fil: Melani, Mariana. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Aubier, Michel. Universite de Paris; Francia
Fil: Boczkowski, Jorge. Universite de Paris; Francia
Fil: Poderoso, Juan José. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Materia
ubiquinol
nitric oxide
endotoxemia
mitochondria
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/118443
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/118443
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Mitochondrial Interplay of Ubiquinol and Nitric Oxide in EndotoxemiaLisdero, Constanza L.Carreras, Maria CeciliaMeulemans, AlainMelani, MarianaAubier, MichelBoczkowski, JorgePoderoso, Juan Joséubiquinolnitric oxideendotoxemiamitochondriahttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3This chapter focuses on role of ubiquinol (UQ) and nitric oxide in endotoxemia. UQ is endogenously synthesized in every organ in a specific pathway branched from cholesterol biosynthesis. Organs have different ubiquinol content depending on the activity of the biosynthetic pathway. In addition to its role as electron carrier in the mitochondrial respiratory chain, UQ exhibits prooxidant and antioxidant properties. The chapter provides the methodological bases that support the relation between UQ content, mitochondrial NO steady-state concentration, and peroxynitrite-mediated mitochondrial damage in representative organs. The chapter also focuses on the contribution of mtNOS to peroxynitrite formation in mitochondria. The chapter presents an experimental model, elaborating endotoxemic animals and sample preparation, and homogenate and mitochondrial preparations. Sources of NO in endotoxemia are also analyzed in the chapter. The chapter discusses the measurement of NOS expression and activity and analyzes the role of ubiquinol in endotoxemia. The endotoxemic mitochondrial damage is also analyzed in the chapter. The chapter elaborates the ubiquinone content and NO-induced hydrogen peroxide production by mitochondria..Fil: Lisdero, Constanza L.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Carreras, Maria Cecilia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Meulemans, Alain. Universite de Paris; FranciaFil: Melani, Mariana. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Aubier, Michel. Universite de Paris; FranciaFil: Boczkowski, Jorge. Universite de Paris; FranciaFil: Poderoso, Juan José. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaElsevier Academic Press Inc2004-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/118443Lisdero, Constanza L.; Carreras, Maria Cecilia; Meulemans, Alain; Melani, Mariana; Aubier, Michel; et al.; The Mitochondrial Interplay of Ubiquinol and Nitric Oxide in Endotoxemia; Elsevier Academic Press Inc; Methods In Enzymology.; 382; 12-2004; 67-810076-6879CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0076-6879(04)82004-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:16:47Zoai:ri.conicet.gov.ar:11336/118443instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:16:47.49CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Mitochondrial Interplay of Ubiquinol and Nitric Oxide in Endotoxemia
title The Mitochondrial Interplay of Ubiquinol and Nitric Oxide in Endotoxemia
spellingShingle The Mitochondrial Interplay of Ubiquinol and Nitric Oxide in Endotoxemia
Lisdero, Constanza L.
ubiquinol
nitric oxide
endotoxemia
mitochondria
title_short The Mitochondrial Interplay of Ubiquinol and Nitric Oxide in Endotoxemia
title_full The Mitochondrial Interplay of Ubiquinol and Nitric Oxide in Endotoxemia
title_fullStr The Mitochondrial Interplay of Ubiquinol and Nitric Oxide in Endotoxemia
title_full_unstemmed The Mitochondrial Interplay of Ubiquinol and Nitric Oxide in Endotoxemia
title_sort The Mitochondrial Interplay of Ubiquinol and Nitric Oxide in Endotoxemia
dc.creator.none.fl_str_mv Lisdero, Constanza L.
Carreras, Maria Cecilia
Meulemans, Alain
Melani, Mariana
Aubier, Michel
Boczkowski, Jorge
Poderoso, Juan José
author Lisdero, Constanza L.
author_facet Lisdero, Constanza L.
Carreras, Maria Cecilia
Meulemans, Alain
Melani, Mariana
Aubier, Michel
Boczkowski, Jorge
Poderoso, Juan José
author_role author
author2 Carreras, Maria Cecilia
Meulemans, Alain
Melani, Mariana
Aubier, Michel
Boczkowski, Jorge
Poderoso, Juan José
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ubiquinol
nitric oxide
endotoxemia
mitochondria
topic ubiquinol
nitric oxide
endotoxemia
mitochondria
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv This chapter focuses on role of ubiquinol (UQ) and nitric oxide in endotoxemia. UQ is endogenously synthesized in every organ in a specific pathway branched from cholesterol biosynthesis. Organs have different ubiquinol content depending on the activity of the biosynthetic pathway. In addition to its role as electron carrier in the mitochondrial respiratory chain, UQ exhibits prooxidant and antioxidant properties. The chapter provides the methodological bases that support the relation between UQ content, mitochondrial NO steady-state concentration, and peroxynitrite-mediated mitochondrial damage in representative organs. The chapter also focuses on the contribution of mtNOS to peroxynitrite formation in mitochondria. The chapter presents an experimental model, elaborating endotoxemic animals and sample preparation, and homogenate and mitochondrial preparations. Sources of NO in endotoxemia are also analyzed in the chapter. The chapter discusses the measurement of NOS expression and activity and analyzes the role of ubiquinol in endotoxemia. The endotoxemic mitochondrial damage is also analyzed in the chapter. The chapter elaborates the ubiquinone content and NO-induced hydrogen peroxide production by mitochondria..
Fil: Lisdero, Constanza L.. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Carreras, Maria Cecilia. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Meulemans, Alain. Universite de Paris; Francia
Fil: Melani, Mariana. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Aubier, Michel. Universite de Paris; Francia
Fil: Boczkowski, Jorge. Universite de Paris; Francia
Fil: Poderoso, Juan José. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
description This chapter focuses on role of ubiquinol (UQ) and nitric oxide in endotoxemia. UQ is endogenously synthesized in every organ in a specific pathway branched from cholesterol biosynthesis. Organs have different ubiquinol content depending on the activity of the biosynthetic pathway. In addition to its role as electron carrier in the mitochondrial respiratory chain, UQ exhibits prooxidant and antioxidant properties. The chapter provides the methodological bases that support the relation between UQ content, mitochondrial NO steady-state concentration, and peroxynitrite-mediated mitochondrial damage in representative organs. The chapter also focuses on the contribution of mtNOS to peroxynitrite formation in mitochondria. The chapter presents an experimental model, elaborating endotoxemic animals and sample preparation, and homogenate and mitochondrial preparations. Sources of NO in endotoxemia are also analyzed in the chapter. The chapter discusses the measurement of NOS expression and activity and analyzes the role of ubiquinol in endotoxemia. The endotoxemic mitochondrial damage is also analyzed in the chapter. The chapter elaborates the ubiquinone content and NO-induced hydrogen peroxide production by mitochondria..
publishDate 2004
dc.date.none.fl_str_mv 2004-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/118443
Lisdero, Constanza L.; Carreras, Maria Cecilia; Meulemans, Alain; Melani, Mariana; Aubier, Michel; et al.; The Mitochondrial Interplay of Ubiquinol and Nitric Oxide in Endotoxemia; Elsevier Academic Press Inc; Methods In Enzymology.; 382; 12-2004; 67-81
0076-6879
CONICET Digital
CONICET
url http://hdl.handle.net/11336/118443
identifier_str_mv Lisdero, Constanza L.; Carreras, Maria Cecilia; Meulemans, Alain; Melani, Mariana; Aubier, Michel; et al.; The Mitochondrial Interplay of Ubiquinol and Nitric Oxide in Endotoxemia; Elsevier Academic Press Inc; Methods In Enzymology.; 382; 12-2004; 67-81
0076-6879
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S0076-6879(04)82004-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Academic Press Inc
publisher.none.fl_str_mv Elsevier Academic Press Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083316503019520
score 13.22299

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