Antibiotic use during pregnancy is linked to offspring gut microbial dysbiosis, barrier disruption, and altered immunity along the gut–lung axis

Autores
Alhasan, Moumen M.; Hölsken, Oliver; Duerr, Claudia; Helfrich, Sofia; Branzk, Nora; Philipp, Alina; Leitz, Dominik; Duerr, Julia; Almousa, Yahia; Barrientos, Gabriela Laura; Mohn, William W.; Gamradt, Stefanie; Conrad, Melanie L.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Antibiotic use during pregnancy is associated with increased asthma risk in children. Since approximately 25% of women use antibiotics during pregnancy, it is important to identify the pathways involved in this phenomenon. We investigate how mother-to-offspring transfer of antibiotic-induced gut microbial dysbiosis influences immune system development along the gut–lung axis. Using a mouse model of maternal antibiotic exposure during pregnancy, we immunophenotyped offspring in early life and after asthma induction. In early life, prenatal-antibiotic exposed offspring exhibited gut microbial dysbiosis, intestinal inflammation (increased fecal lipocalin-2 and IgA), and dysregulated intestinal ILC3 subtypes. Intestinal barrier dysfunction in the offspring was indicated by a FITC-dextran intestinal permeability assay and circulating lipopolysaccharide. This was accompanied by increased T-helper (Th)17 cell percentages in the offspring's blood and lungs in both early life and after allergy induction. Lung tissue additionally showed increased percentages of RORγt T-regulatory (Treg) cells at both time points. Our investigation of the gut–lung axis identifies early-life gut dysbiosis, intestinal inflammation, and barrier dysfunction as a possible developmental programming event promoting increased expression of RORγt in blood and lung CD4+ T cells that may contribute to increased asthma risk.
Fil: Alhasan, Moumen M.. Universität zu Berlin; Alemania
Fil: Hölsken, Oliver. Freie Universität Berlin; Alemania
Fil: Duerr, Claudia. Freie Universität Berlin; Alemania
Fil: Helfrich, Sofia. Freie Universität Berlin; Alemania
Fil: Branzk, Nora. Freie Universität Berlin; Alemania
Fil: Philipp, Alina. Freie Universität Berlin; Alemania
Fil: Leitz, Dominik. Freie Universität Berlin; Alemania
Fil: Duerr, Julia. Freie Universität Berlin; Alemania
Fil: Almousa, Yahia. Freie Universität Berlin; Alemania
Fil: Barrientos, Gabriela Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman; Argentina
Fil: Mohn, William W.. University of British Columbia; Canadá
Fil: Gamradt, Stefanie. Freie Universität Berlin; Alemania
Fil: Conrad, Melanie L.. Freie Universität Berlin; Alemania
Materia
ANTIBIOTICS
ASTHMA
GUT–LUNG AXIS
PREGNANCY
TH17 CELL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/219931
Acceder
id CONICETDig_47bee8db2fe5d454f5f971c35f8a4672
oai_identifier_str oai:ri.conicet.gov.ar:11336/219931
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antibiotic use during pregnancy is linked to offspring gut microbial dysbiosis, barrier disruption, and altered immunity along the gut–lung axisAlhasan, Moumen M.Hölsken, OliverDuerr, ClaudiaHelfrich, SofiaBranzk, NoraPhilipp, AlinaLeitz, DominikDuerr, JuliaAlmousa, YahiaBarrientos, Gabriela LauraMohn, William W.Gamradt, StefanieConrad, Melanie L.ANTIBIOTICSASTHMAGUT–LUNG AXISPREGNANCYTH17 CELLhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Antibiotic use during pregnancy is associated with increased asthma risk in children. Since approximately 25% of women use antibiotics during pregnancy, it is important to identify the pathways involved in this phenomenon. We investigate how mother-to-offspring transfer of antibiotic-induced gut microbial dysbiosis influences immune system development along the gut–lung axis. Using a mouse model of maternal antibiotic exposure during pregnancy, we immunophenotyped offspring in early life and after asthma induction. In early life, prenatal-antibiotic exposed offspring exhibited gut microbial dysbiosis, intestinal inflammation (increased fecal lipocalin-2 and IgA), and dysregulated intestinal ILC3 subtypes. Intestinal barrier dysfunction in the offspring was indicated by a FITC-dextran intestinal permeability assay and circulating lipopolysaccharide. This was accompanied by increased T-helper (Th)17 cell percentages in the offspring's blood and lungs in both early life and after allergy induction. Lung tissue additionally showed increased percentages of RORγt T-regulatory (Treg) cells at both time points. Our investigation of the gut–lung axis identifies early-life gut dysbiosis, intestinal inflammation, and barrier dysfunction as a possible developmental programming event promoting increased expression of RORγt in blood and lung CD4+ T cells that may contribute to increased asthma risk.Fil: Alhasan, Moumen M.. Universität zu Berlin; AlemaniaFil: Hölsken, Oliver. Freie Universität Berlin; AlemaniaFil: Duerr, Claudia. Freie Universität Berlin; AlemaniaFil: Helfrich, Sofia. Freie Universität Berlin; AlemaniaFil: Branzk, Nora. Freie Universität Berlin; AlemaniaFil: Philipp, Alina. Freie Universität Berlin; AlemaniaFil: Leitz, Dominik. Freie Universität Berlin; AlemaniaFil: Duerr, Julia. Freie Universität Berlin; AlemaniaFil: Almousa, Yahia. Freie Universität Berlin; AlemaniaFil: Barrientos, Gabriela Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman; ArgentinaFil: Mohn, William W.. University of British Columbia; CanadáFil: Gamradt, Stefanie. Freie Universität Berlin; AlemaniaFil: Conrad, Melanie L.. Freie Universität Berlin; AlemaniaWiley VCH Verlag2023-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/219931Alhasan, Moumen M.; Hölsken, Oliver; Duerr, Claudia; Helfrich, Sofia; Branzk, Nora; et al.; Antibiotic use during pregnancy is linked to offspring gut microbial dysbiosis, barrier disruption, and altered immunity along the gut–lung axis; Wiley VCH Verlag; European Journal of Immunology; 53; 10; 7-2023; 1-150014-2980CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/eji.202350394info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:39:59Zoai:ri.conicet.gov.ar:11336/219931instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:39:59.726CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibiotic use during pregnancy is linked to offspring gut microbial dysbiosis, barrier disruption, and altered immunity along the gut–lung axis
title Antibiotic use during pregnancy is linked to offspring gut microbial dysbiosis, barrier disruption, and altered immunity along the gut–lung axis
spellingShingle Antibiotic use during pregnancy is linked to offspring gut microbial dysbiosis, barrier disruption, and altered immunity along the gut–lung axis
Alhasan, Moumen M.
ANTIBIOTICS
ASTHMA
GUT–LUNG AXIS
PREGNANCY
TH17 CELL
title_short Antibiotic use during pregnancy is linked to offspring gut microbial dysbiosis, barrier disruption, and altered immunity along the gut–lung axis
title_full Antibiotic use during pregnancy is linked to offspring gut microbial dysbiosis, barrier disruption, and altered immunity along the gut–lung axis
title_fullStr Antibiotic use during pregnancy is linked to offspring gut microbial dysbiosis, barrier disruption, and altered immunity along the gut–lung axis
title_full_unstemmed Antibiotic use during pregnancy is linked to offspring gut microbial dysbiosis, barrier disruption, and altered immunity along the gut–lung axis
title_sort Antibiotic use during pregnancy is linked to offspring gut microbial dysbiosis, barrier disruption, and altered immunity along the gut–lung axis
dc.creator.none.fl_str_mv Alhasan, Moumen M.
Hölsken, Oliver
Duerr, Claudia
Helfrich, Sofia
Branzk, Nora
Philipp, Alina
Leitz, Dominik
Duerr, Julia
Almousa, Yahia
Barrientos, Gabriela Laura
Mohn, William W.
Gamradt, Stefanie
Conrad, Melanie L.
author Alhasan, Moumen M.
author_facet Alhasan, Moumen M.
Hölsken, Oliver
Duerr, Claudia
Helfrich, Sofia
Branzk, Nora
Philipp, Alina
Leitz, Dominik
Duerr, Julia
Almousa, Yahia
Barrientos, Gabriela Laura
Mohn, William W.
Gamradt, Stefanie
Conrad, Melanie L.
author_role author
author2 Hölsken, Oliver
Duerr, Claudia
Helfrich, Sofia
Branzk, Nora
Philipp, Alina
Leitz, Dominik
Duerr, Julia
Almousa, Yahia
Barrientos, Gabriela Laura
Mohn, William W.
Gamradt, Stefanie
Conrad, Melanie L.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIBIOTICS
ASTHMA
GUT–LUNG AXIS
PREGNANCY
TH17 CELL
topic ANTIBIOTICS
ASTHMA
GUT–LUNG AXIS
PREGNANCY
TH17 CELL
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Antibiotic use during pregnancy is associated with increased asthma risk in children. Since approximately 25% of women use antibiotics during pregnancy, it is important to identify the pathways involved in this phenomenon. We investigate how mother-to-offspring transfer of antibiotic-induced gut microbial dysbiosis influences immune system development along the gut–lung axis. Using a mouse model of maternal antibiotic exposure during pregnancy, we immunophenotyped offspring in early life and after asthma induction. In early life, prenatal-antibiotic exposed offspring exhibited gut microbial dysbiosis, intestinal inflammation (increased fecal lipocalin-2 and IgA), and dysregulated intestinal ILC3 subtypes. Intestinal barrier dysfunction in the offspring was indicated by a FITC-dextran intestinal permeability assay and circulating lipopolysaccharide. This was accompanied by increased T-helper (Th)17 cell percentages in the offspring's blood and lungs in both early life and after allergy induction. Lung tissue additionally showed increased percentages of RORγt T-regulatory (Treg) cells at both time points. Our investigation of the gut–lung axis identifies early-life gut dysbiosis, intestinal inflammation, and barrier dysfunction as a possible developmental programming event promoting increased expression of RORγt in blood and lung CD4+ T cells that may contribute to increased asthma risk.
Fil: Alhasan, Moumen M.. Universität zu Berlin; Alemania
Fil: Hölsken, Oliver. Freie Universität Berlin; Alemania
Fil: Duerr, Claudia. Freie Universität Berlin; Alemania
Fil: Helfrich, Sofia. Freie Universität Berlin; Alemania
Fil: Branzk, Nora. Freie Universität Berlin; Alemania
Fil: Philipp, Alina. Freie Universität Berlin; Alemania
Fil: Leitz, Dominik. Freie Universität Berlin; Alemania
Fil: Duerr, Julia. Freie Universität Berlin; Alemania
Fil: Almousa, Yahia. Freie Universität Berlin; Alemania
Fil: Barrientos, Gabriela Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Aleman; Argentina
Fil: Mohn, William W.. University of British Columbia; Canadá
Fil: Gamradt, Stefanie. Freie Universität Berlin; Alemania
Fil: Conrad, Melanie L.. Freie Universität Berlin; Alemania
description Antibiotic use during pregnancy is associated with increased asthma risk in children. Since approximately 25% of women use antibiotics during pregnancy, it is important to identify the pathways involved in this phenomenon. We investigate how mother-to-offspring transfer of antibiotic-induced gut microbial dysbiosis influences immune system development along the gut–lung axis. Using a mouse model of maternal antibiotic exposure during pregnancy, we immunophenotyped offspring in early life and after asthma induction. In early life, prenatal-antibiotic exposed offspring exhibited gut microbial dysbiosis, intestinal inflammation (increased fecal lipocalin-2 and IgA), and dysregulated intestinal ILC3 subtypes. Intestinal barrier dysfunction in the offspring was indicated by a FITC-dextran intestinal permeability assay and circulating lipopolysaccharide. This was accompanied by increased T-helper (Th)17 cell percentages in the offspring's blood and lungs in both early life and after allergy induction. Lung tissue additionally showed increased percentages of RORγt T-regulatory (Treg) cells at both time points. Our investigation of the gut–lung axis identifies early-life gut dysbiosis, intestinal inflammation, and barrier dysfunction as a possible developmental programming event promoting increased expression of RORγt in blood and lung CD4+ T cells that may contribute to increased asthma risk.
publishDate 2023
dc.date.none.fl_str_mv 2023-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/219931
Alhasan, Moumen M.; Hölsken, Oliver; Duerr, Claudia; Helfrich, Sofia; Branzk, Nora; et al.; Antibiotic use during pregnancy is linked to offspring gut microbial dysbiosis, barrier disruption, and altered immunity along the gut–lung axis; Wiley VCH Verlag; European Journal of Immunology; 53; 10; 7-2023; 1-15
0014-2980
CONICET Digital
CONICET
url http://hdl.handle.net/11336/219931
identifier_str_mv Alhasan, Moumen M.; Hölsken, Oliver; Duerr, Claudia; Helfrich, Sofia; Branzk, Nora; et al.; Antibiotic use during pregnancy is linked to offspring gut microbial dysbiosis, barrier disruption, and altered immunity along the gut–lung axis; Wiley VCH Verlag; European Journal of Immunology; 53; 10; 7-2023; 1-15
0014-2980
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/eji.202350394
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614426632650752
score 13.070432

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