Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (P...

Autores: Villar, Luis; Lienqueo, Ixia; Llanes, Analia Susana; Rojas, Pamela; Perez, Jorge; Correa, Francisco; Sagredo, Boris; Masciarelli, Oscar Alberto; Luna, Maria Virginia; Almada, Rubén
Año de publicación: 2020
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: In sweet cherry trees, flowering is commercially important because the flowers, after fertilization, will generate the fruits. In P. avium, the flowering induction and flower organogensis are the first developmental steps towards flower formation and they occur within specialized organs known as floral buds during the summer, nine months before blooming. During this period the number of floral buds per tree and the bud fruitfulness (number of flowers per bud) are stablished affecting the potential yield of orchards and the plant architecture. The floral bud development is sensitive to any type of stress and the hotter and drier summers will interfere with this process and are calling for new adapted cultivars. A better understanding of the underlying molecular and hormonal mechanisms would be of help, but unlike the model plant Arabidopsis, very little is known about floral induction in sweet cherry. To explore the molecular mechanism of floral bud differentiation, high-throughput RNA sequencing was used to detect differences in the gene expression of P. avium floral buds at five differentiation stages. We found 2,982 differentially expressed genes during floral bud development. We identified genes associated with floral initiation or floral organ identity that appear to be useful biomarkers of floral development and several transcription factor families (ERF, MYB, bHLH, MADS-box and NAC gene family) with novel potential roles during floral transition in this species. We analyzed in deep the MADS-box gene family and we shed light about their key role during floral bud and organs development in P. avium. Furthermore, the hormonal-related signatures in the gene regulatory networks and the dynamic changes of absicic acid, zeatin and indolacetic acid contents in buds suggest an important role for these hormones during floral bud differentiation in sweet cherry. These data provide a rich source of novel informacion for functional and evolutionary studies about floral bud development in sweet cherry and new tools for biotechnology and breeding.
Fil: Villar, Luis. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile
Fil: Lienqueo, Ixia. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile
Fil: Llanes, Analia Susana. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Rojas, Pamela. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile
Fil: Perez, Jorge. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile
Fil: Correa, Francisco. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile
Fil: Sagredo, Boris. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile
Fil: Masciarelli, Oscar Alberto. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Luna, Maria Virginia. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Almada, Rubén. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile
Materia: BUDS
FLOWERS
TRANSCRIPTION FACTORS
CHERRIES
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/144545

Acceder

id	CONICETDig_47883f786e77737f7de16ecae670fb76
oai_identifier_str	oai:ri.conicet.gov.ar:11336/144545
network_acronym_str	CONICETDig
repository_id_str	3498
network_name_str	CONICET Digital (CONICET)
spelling	Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. Cv. Bing)Villar, LuisLienqueo, IxiaLlanes, Analia SusanaRojas, PamelaPerez, JorgeCorrea, FranciscoSagredo, BorisMasciarelli, Oscar AlbertoLuna, Maria VirginiaAlmada, RubénBUDSFLOWERSTRANSCRIPTION FACTORSCHERRIEShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4In sweet cherry trees, flowering is commercially important because the flowers, after fertilization, will generate the fruits. In P. avium, the flowering induction and flower organogensis are the first developmental steps towards flower formation and they occur within specialized organs known as floral buds during the summer, nine months before blooming. During this period the number of floral buds per tree and the bud fruitfulness (number of flowers per bud) are stablished affecting the potential yield of orchards and the plant architecture. The floral bud development is sensitive to any type of stress and the hotter and drier summers will interfere with this process and are calling for new adapted cultivars. A better understanding of the underlying molecular and hormonal mechanisms would be of help, but unlike the model plant Arabidopsis, very little is known about floral induction in sweet cherry. To explore the molecular mechanism of floral bud differentiation, high-throughput RNA sequencing was used to detect differences in the gene expression of P. avium floral buds at five differentiation stages. We found 2,982 differentially expressed genes during floral bud development. We identified genes associated with floral initiation or floral organ identity that appear to be useful biomarkers of floral development and several transcription factor families (ERF, MYB, bHLH, MADS-box and NAC gene family) with novel potential roles during floral transition in this species. We analyzed in deep the MADS-box gene family and we shed light about their key role during floral bud and organs development in P. avium. Furthermore, the hormonal-related signatures in the gene regulatory networks and the dynamic changes of absicic acid, zeatin and indolacetic acid contents in buds suggest an important role for these hormones during floral bud differentiation in sweet cherry. These data provide a rich source of novel informacion for functional and evolutionary studies about floral bud development in sweet cherry and new tools for biotechnology and breeding.Fil: Villar, Luis. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Lienqueo, Ixia. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Llanes, Analia Susana. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Rojas, Pamela. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Perez, Jorge. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Correa, Francisco. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Sagredo, Boris. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChileFil: Masciarelli, Oscar Alberto. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Luna, Maria Virginia. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Almada, Rubén. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; ChilePublic Library of Science2020-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/144545Villar, Luis; Lienqueo, Ixia; Llanes, Analia Susana; Rojas, Pamela; Perez, Jorge; et al.; Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. Cv. Bing); Public Library of Science; Plos One; 15; 3; 3-2020; 1-281932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0230110info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230110info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:30:15Zoai:ri.conicet.gov.ar:11336/144545instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:30:16.154CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. Cv. Bing)
title	Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. Cv. Bing)
spellingShingle	Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. Cv. Bing) Villar, Luis BUDS FLOWERS TRANSCRIPTION FACTORS CHERRIES
title_short	Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. Cv. Bing)
title_full	Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. Cv. Bing)
title_fullStr	Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. Cv. Bing)
title_full_unstemmed	Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. Cv. Bing)
title_sort	Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. Cv. Bing)
dc.creator.none.fl_str_mv	Villar, Luis Lienqueo, Ixia Llanes, Analia Susana Rojas, Pamela Perez, Jorge Correa, Francisco Sagredo, Boris Masciarelli, Oscar Alberto Luna, Maria Virginia Almada, Rubén
author	Villar, Luis
author_facet	Villar, Luis Lienqueo, Ixia Llanes, Analia Susana Rojas, Pamela Perez, Jorge Correa, Francisco Sagredo, Boris Masciarelli, Oscar Alberto Luna, Maria Virginia Almada, Rubén
author_role	author
author2	Lienqueo, Ixia Llanes, Analia Susana Rojas, Pamela Perez, Jorge Correa, Francisco Sagredo, Boris Masciarelli, Oscar Alberto Luna, Maria Virginia Almada, Rubén
author2_role	author author author author author author author author author
dc.subject.none.fl_str_mv	BUDS FLOWERS TRANSCRIPTION FACTORS CHERRIES
topic	BUDS FLOWERS TRANSCRIPTION FACTORS CHERRIES
purl_subject.fl_str_mv	https://purl.org/becyt/ford/4.4 https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv	In sweet cherry trees, flowering is commercially important because the flowers, after fertilization, will generate the fruits. In P. avium, the flowering induction and flower organogensis are the first developmental steps towards flower formation and they occur within specialized organs known as floral buds during the summer, nine months before blooming. During this period the number of floral buds per tree and the bud fruitfulness (number of flowers per bud) are stablished affecting the potential yield of orchards and the plant architecture. The floral bud development is sensitive to any type of stress and the hotter and drier summers will interfere with this process and are calling for new adapted cultivars. A better understanding of the underlying molecular and hormonal mechanisms would be of help, but unlike the model plant Arabidopsis, very little is known about floral induction in sweet cherry. To explore the molecular mechanism of floral bud differentiation, high-throughput RNA sequencing was used to detect differences in the gene expression of P. avium floral buds at five differentiation stages. We found 2,982 differentially expressed genes during floral bud development. We identified genes associated with floral initiation or floral organ identity that appear to be useful biomarkers of floral development and several transcription factor families (ERF, MYB, bHLH, MADS-box and NAC gene family) with novel potential roles during floral transition in this species. We analyzed in deep the MADS-box gene family and we shed light about their key role during floral bud and organs development in P. avium. Furthermore, the hormonal-related signatures in the gene regulatory networks and the dynamic changes of absicic acid, zeatin and indolacetic acid contents in buds suggest an important role for these hormones during floral bud differentiation in sweet cherry. These data provide a rich source of novel informacion for functional and evolutionary studies about floral bud development in sweet cherry and new tools for biotechnology and breeding. Fil: Villar, Luis. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile Fil: Lienqueo, Ixia. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile Fil: Llanes, Analia Susana. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Rojas, Pamela. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile Fil: Perez, Jorge. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile Fil: Correa, Francisco. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile Fil: Sagredo, Boris. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile Fil: Masciarelli, Oscar Alberto. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Luna, Maria Virginia. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina Fil: Almada, Rubén. Comision Nacional de Investigacion Cientifica y Tecnologica. Centro de Investigacion Regional. Centro de Estudios Avanzados En Fruticultura.; Chile
description	In sweet cherry trees, flowering is commercially important because the flowers, after fertilization, will generate the fruits. In P. avium, the flowering induction and flower organogensis are the first developmental steps towards flower formation and they occur within specialized organs known as floral buds during the summer, nine months before blooming. During this period the number of floral buds per tree and the bud fruitfulness (number of flowers per bud) are stablished affecting the potential yield of orchards and the plant architecture. The floral bud development is sensitive to any type of stress and the hotter and drier summers will interfere with this process and are calling for new adapted cultivars. A better understanding of the underlying molecular and hormonal mechanisms would be of help, but unlike the model plant Arabidopsis, very little is known about floral induction in sweet cherry. To explore the molecular mechanism of floral bud differentiation, high-throughput RNA sequencing was used to detect differences in the gene expression of P. avium floral buds at five differentiation stages. We found 2,982 differentially expressed genes during floral bud development. We identified genes associated with floral initiation or floral organ identity that appear to be useful biomarkers of floral development and several transcription factor families (ERF, MYB, bHLH, MADS-box and NAC gene family) with novel potential roles during floral transition in this species. We analyzed in deep the MADS-box gene family and we shed light about their key role during floral bud and organs development in P. avium. Furthermore, the hormonal-related signatures in the gene regulatory networks and the dynamic changes of absicic acid, zeatin and indolacetic acid contents in buds suggest an important role for these hormones during floral bud differentiation in sweet cherry. These data provide a rich source of novel informacion for functional and evolutionary studies about floral bud development in sweet cherry and new tools for biotechnology and breeding.
publishDate	2020
dc.date.none.fl_str_mv	2020-03
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/144545 Villar, Luis; Lienqueo, Ixia; Llanes, Analia Susana; Rojas, Pamela; Perez, Jorge; et al.; Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. Cv. Bing); Public Library of Science; Plos One; 15; 3; 3-2020; 1-28 1932-6203 CONICET Digital CONICET
url	http://hdl.handle.net/11336/144545
identifier_str_mv	Villar, Luis; Lienqueo, Ixia; Llanes, Analia Susana; Rojas, Pamela; Perez, Jorge; et al.; Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. Cv. Bing); Public Library of Science; Plos One; 15; 3; 3-2020; 1-28 1932-6203 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0230110 info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0230110
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Public Library of Science
publisher.none.fl_str_mv	Public Library of Science
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_	1846781894957465600
score	12.982451

Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (P...

Publicaciones similares