Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species

Autores
Acuña, Cintia Vanesa; Villalba, Pamela Victoria; Hopp, Horacio Esteban; Marcucci Poltri, Susana Noemí
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
AIM OF STUDY: To analyze the feasibility of extrapolating conclusions on wood quality genetic control between different Eucalyptus species, particularly from species with better genomic information, to those less characterized. For this purpose, the first step is to analyze the conservation and cross-transferability of microsatellites markers (SSRs) located in candidate genes. AREA OF STUDY: Eucalyptus species implanted in Argentina coming from different Australian origins. MATERIALS AND METHODS: Twelve validated and polymorphic SSRs in candidate genes (SSR-CGs) for wood quality in E. globulus were selected for cross species amplification in six species: E. grandis, E. saligna, E. dunnii, E. viminalis, E. camaldulensis and E. tereticornis. MAIN RESULTS: High cross-species transferability (92% to 100%) was found for the 12 polymorphic SSRs detected in E. globulus. These markers revealed allelic diversity in nine important candidate genes: cinnamoyl CoA reductase (CCR), cellulose synthase 3 (CesA3), the transcription factor LIM1, homocysteine S-methyltransferase (HMT), shikimate kinase (SK), xyloglucan endotransglycosylase 2 (XTH2), glutathione S-transferase (GST), glutamate decarboxylase (GAD) and peroxidase (PER). RESEARCH HIGHLIGHTS: The markers described are potentially suitable for comparative QTL mapping, molecular marker assisted breeding (MAB) and for population genetic studies across different species within the subgenus Symphyomyrtus.
Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Villalba, Pamela Victoria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Marcucci Poltri, Susana Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; Argentina
Materia
microsatélites
calidad madera
Eucaliptus
Symphyomyrtus
Validation
Cross-transferability
SSR
Functional markers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/35131
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/35131
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus speciesAcuña, Cintia VanesaVillalba, Pamela VictoriaHopp, Horacio EstebanMarcucci Poltri, Susana Noemímicrosatélitescalidad maderaEucaliptusSymphyomyrtusValidationCross-transferabilitySSRFunctional markershttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2AIM OF STUDY: To analyze the feasibility of extrapolating conclusions on wood quality genetic control between different Eucalyptus species, particularly from species with better genomic information, to those less characterized. For this purpose, the first step is to analyze the conservation and cross-transferability of microsatellites markers (SSRs) located in candidate genes. AREA OF STUDY: Eucalyptus species implanted in Argentina coming from different Australian origins. MATERIALS AND METHODS: Twelve validated and polymorphic SSRs in candidate genes (SSR-CGs) for wood quality in E. globulus were selected for cross species amplification in six species: E. grandis, E. saligna, E. dunnii, E. viminalis, E. camaldulensis and E. tereticornis. MAIN RESULTS: High cross-species transferability (92% to 100%) was found for the 12 polymorphic SSRs detected in E. globulus. These markers revealed allelic diversity in nine important candidate genes: cinnamoyl CoA reductase (CCR), cellulose synthase 3 (CesA3), the transcription factor LIM1, homocysteine S-methyltransferase (HMT), shikimate kinase (SK), xyloglucan endotransglycosylase 2 (XTH2), glutathione S-transferase (GST), glutamate decarboxylase (GAD) and peroxidase (PER). RESEARCH HIGHLIGHTS: The markers described are potentially suitable for comparative QTL mapping, molecular marker assisted breeding (MAB) and for population genetic studies across different species within the subgenus Symphyomyrtus.Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Villalba, Pamela Victoria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Marcucci Poltri, Susana Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; ArgentinaInstituto Nacional de Investigación y Tecnología Agraria y Alimentaria2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/35131Acuña, Cintia Vanesa; Villalba, Pamela Victoria; Hopp, Horacio Esteban; Marcucci Poltri, Susana Noemí; Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species; Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; Forest Systems; 23; 3; 3-2014; 506-5122171-50682171-9845CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://revistas.inia.es/index.php/fs/article/view/5279info:eu-repo/semantics/altIdentifier/url/https://dialnet.unirioja.es/servlet/articulo?codigo=6240019info:eu-repo/semantics/altIdentifier/doi/10.5424/fs/2014233-05279info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:51:15Zoai:ri.conicet.gov.ar:11336/35131instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:51:15.254CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species
title Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species
spellingShingle Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species
Acuña, Cintia Vanesa
microsatélites
calidad madera
Eucaliptus
Symphyomyrtus
Validation
Cross-transferability
SSR
Functional markers
title_short Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species
title_full Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species
title_fullStr Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species
title_full_unstemmed Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species
title_sort Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species
dc.creator.none.fl_str_mv Acuña, Cintia Vanesa
Villalba, Pamela Victoria
Hopp, Horacio Esteban
Marcucci Poltri, Susana Noemí
author Acuña, Cintia Vanesa
author_facet Acuña, Cintia Vanesa
Villalba, Pamela Victoria
Hopp, Horacio Esteban
Marcucci Poltri, Susana Noemí
author_role author
author2 Villalba, Pamela Victoria
Hopp, Horacio Esteban
Marcucci Poltri, Susana Noemí
author2_role author
author
author
dc.subject.none.fl_str_mv microsatélites
calidad madera
Eucaliptus
Symphyomyrtus
Validation
Cross-transferability
SSR
Functional markers
topic microsatélites
calidad madera
Eucaliptus
Symphyomyrtus
Validation
Cross-transferability
SSR
Functional markers
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv AIM OF STUDY: To analyze the feasibility of extrapolating conclusions on wood quality genetic control between different Eucalyptus species, particularly from species with better genomic information, to those less characterized. For this purpose, the first step is to analyze the conservation and cross-transferability of microsatellites markers (SSRs) located in candidate genes. AREA OF STUDY: Eucalyptus species implanted in Argentina coming from different Australian origins. MATERIALS AND METHODS: Twelve validated and polymorphic SSRs in candidate genes (SSR-CGs) for wood quality in E. globulus were selected for cross species amplification in six species: E. grandis, E. saligna, E. dunnii, E. viminalis, E. camaldulensis and E. tereticornis. MAIN RESULTS: High cross-species transferability (92% to 100%) was found for the 12 polymorphic SSRs detected in E. globulus. These markers revealed allelic diversity in nine important candidate genes: cinnamoyl CoA reductase (CCR), cellulose synthase 3 (CesA3), the transcription factor LIM1, homocysteine S-methyltransferase (HMT), shikimate kinase (SK), xyloglucan endotransglycosylase 2 (XTH2), glutathione S-transferase (GST), glutamate decarboxylase (GAD) and peroxidase (PER). RESEARCH HIGHLIGHTS: The markers described are potentially suitable for comparative QTL mapping, molecular marker assisted breeding (MAB) and for population genetic studies across different species within the subgenus Symphyomyrtus.
Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Villalba, Pamela Victoria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Marcucci Poltri, Susana Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Laboratorio de Agrobiotecnología; Argentina
description AIM OF STUDY: To analyze the feasibility of extrapolating conclusions on wood quality genetic control between different Eucalyptus species, particularly from species with better genomic information, to those less characterized. For this purpose, the first step is to analyze the conservation and cross-transferability of microsatellites markers (SSRs) located in candidate genes. AREA OF STUDY: Eucalyptus species implanted in Argentina coming from different Australian origins. MATERIALS AND METHODS: Twelve validated and polymorphic SSRs in candidate genes (SSR-CGs) for wood quality in E. globulus were selected for cross species amplification in six species: E. grandis, E. saligna, E. dunnii, E. viminalis, E. camaldulensis and E. tereticornis. MAIN RESULTS: High cross-species transferability (92% to 100%) was found for the 12 polymorphic SSRs detected in E. globulus. These markers revealed allelic diversity in nine important candidate genes: cinnamoyl CoA reductase (CCR), cellulose synthase 3 (CesA3), the transcription factor LIM1, homocysteine S-methyltransferase (HMT), shikimate kinase (SK), xyloglucan endotransglycosylase 2 (XTH2), glutathione S-transferase (GST), glutamate decarboxylase (GAD) and peroxidase (PER). RESEARCH HIGHLIGHTS: The markers described are potentially suitable for comparative QTL mapping, molecular marker assisted breeding (MAB) and for population genetic studies across different species within the subgenus Symphyomyrtus.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/35131
Acuña, Cintia Vanesa; Villalba, Pamela Victoria; Hopp, Horacio Esteban; Marcucci Poltri, Susana Noemí; Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species; Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; Forest Systems; 23; 3; 3-2014; 506-512
2171-5068
2171-9845
CONICET Digital
CONICET
url http://hdl.handle.net/11336/35131
identifier_str_mv Acuña, Cintia Vanesa; Villalba, Pamela Victoria; Hopp, Horacio Esteban; Marcucci Poltri, Susana Noemí; Transferability of microsatellite markers located in candidate genes for wood properties between Eucalyptus species; Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria; Forest Systems; 23; 3; 3-2014; 506-512
2171-5068
2171-9845
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://revistas.inia.es/index.php/fs/article/view/5279
info:eu-repo/semantics/altIdentifier/url/https://dialnet.unirioja.es/servlet/articulo?codigo=6240019
info:eu-repo/semantics/altIdentifier/doi/10.5424/fs/2014233-05279
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
publisher.none.fl_str_mv Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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