Antibacterial activity of fractions obtained from stem bark of geoffroea decorticans tincture

Autores
Pastoriza, Ana Cristina; Iriarte, María Luz; Soberon, Jose Rodolfo; Sgariglia, Melina Araceli
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Geoffroea decorticans, popularly known as "chañar", is traditionally used as anti-inflammatory, to treat infected wounds and dysentery. Chañar bark is an external tissue known for its medicinal properties, but its bioactive components were not reported yet. Objectives: To evaluate the antibacterial activity of polyphenolics (PG) and triterpenoids (TG) enriched fractions obtained from Geoffroea decorticans bark (CC) against Escherichia coli and Staphylococcus aureus. Methodology: CC grounded powder was washed with n-hexane and extracted with methanol. The extract was dried and fractionated by precipitation with acetone to separate TG from PG. The extracted material (EM) was determined for TG and PG. Antibacterial activity was evaluated against E. Coli (ATCC 25922) and S. aureus (ATCC 29213) by dot-blot bioautography and disk diffusion assay. Samples were assayed at 250 - 750 μg of EM, Ciprofloxacin was used as control. The plates were incubated at 37° C for 24 h. The bioactive fractions were analyzed by GC-MS. Results: PG (250 μg) was active against both strains. The inhibitory halo obtained against S. aureus was significant higher than for E. coli (p>0.05). TG did not showed activity against the assayed strains. GC-MS experiments performed for PG showed the presence of Dihydroquercetin, 3,4´-Dimethoxy-5,7,3´-trihydroxyflavone and protocatechic acid. Conclusions: CC exhibited antibacterial activity against Gram positive and Gram negative strains, which support ethnomedicinal use. This activity could be attributed to the phenolic compounds constituents.
Fil: Pastoriza, Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina
Fil: Iriarte, María Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina
Fil: Soberon, Jose Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina
Fil: Sgariglia, Melina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina
XXXVI Annual Scientific Meeting of the Tucuman Biology Association
Tafí Viejo
Argentina
Asociación de Biología de Tucumán
Materia
GEOFFROEA DECORTICANS
BURK
ANTIBACTERIAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/280956
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/280956
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antibacterial activity of fractions obtained from stem bark of geoffroea decorticans tincturePastoriza, Ana CristinaIriarte, María LuzSoberon, Jose RodolfoSgariglia, Melina AraceliGEOFFROEA DECORTICANSBURKANTIBACTERIALhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Geoffroea decorticans, popularly known as "chañar", is traditionally used as anti-inflammatory, to treat infected wounds and dysentery. Chañar bark is an external tissue known for its medicinal properties, but its bioactive components were not reported yet. Objectives: To evaluate the antibacterial activity of polyphenolics (PG) and triterpenoids (TG) enriched fractions obtained from Geoffroea decorticans bark (CC) against Escherichia coli and Staphylococcus aureus. Methodology: CC grounded powder was washed with n-hexane and extracted with methanol. The extract was dried and fractionated by precipitation with acetone to separate TG from PG. The extracted material (EM) was determined for TG and PG. Antibacterial activity was evaluated against E. Coli (ATCC 25922) and S. aureus (ATCC 29213) by dot-blot bioautography and disk diffusion assay. Samples were assayed at 250 - 750 μg of EM, Ciprofloxacin was used as control. The plates were incubated at 37° C for 24 h. The bioactive fractions were analyzed by GC-MS. Results: PG (250 μg) was active against both strains. The inhibitory halo obtained against S. aureus was significant higher than for E. coli (p>0.05). TG did not showed activity against the assayed strains. GC-MS experiments performed for PG showed the presence of Dihydroquercetin, 3,4´-Dimethoxy-5,7,3´-trihydroxyflavone and protocatechic acid. Conclusions: CC exhibited antibacterial activity against Gram positive and Gram negative strains, which support ethnomedicinal use. This activity could be attributed to the phenolic compounds constituents.Fil: Pastoriza, Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; ArgentinaFil: Iriarte, María Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; ArgentinaFil: Soberon, Jose Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; ArgentinaFil: Sgariglia, Melina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; ArgentinaXXXVI Annual Scientific Meeting of the Tucuman Biology AssociationTafí ViejoArgentinaAsociación de Biología de TucumánTech Science Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/280956Antibacterial activity of fractions obtained from stem bark of geoffroea decorticans tincture; XXXVI Annual Scientific Meeting of the Tucuman Biology Association; Tafí Viejo; Argentina; 2019; 25-250327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v44nSuppl.2Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T12:54:44Zoai:ri.conicet.gov.ar:11336/280956instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 12:54:45.133CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibacterial activity of fractions obtained from stem bark of geoffroea decorticans tincture
title Antibacterial activity of fractions obtained from stem bark of geoffroea decorticans tincture
spellingShingle Antibacterial activity of fractions obtained from stem bark of geoffroea decorticans tincture
Pastoriza, Ana Cristina
GEOFFROEA DECORTICANS
BURK
ANTIBACTERIAL
title_short Antibacterial activity of fractions obtained from stem bark of geoffroea decorticans tincture
title_full Antibacterial activity of fractions obtained from stem bark of geoffroea decorticans tincture
title_fullStr Antibacterial activity of fractions obtained from stem bark of geoffroea decorticans tincture
title_full_unstemmed Antibacterial activity of fractions obtained from stem bark of geoffroea decorticans tincture
title_sort Antibacterial activity of fractions obtained from stem bark of geoffroea decorticans tincture
dc.creator.none.fl_str_mv Pastoriza, Ana Cristina
Iriarte, María Luz
Soberon, Jose Rodolfo
Sgariglia, Melina Araceli
author Pastoriza, Ana Cristina
author_facet Pastoriza, Ana Cristina
Iriarte, María Luz
Soberon, Jose Rodolfo
Sgariglia, Melina Araceli
author_role author
author2 Iriarte, María Luz
Soberon, Jose Rodolfo
Sgariglia, Melina Araceli
author2_role author
author
author
dc.subject.none.fl_str_mv GEOFFROEA DECORTICANS
BURK
ANTIBACTERIAL
topic GEOFFROEA DECORTICANS
BURK
ANTIBACTERIAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Geoffroea decorticans, popularly known as "chañar", is traditionally used as anti-inflammatory, to treat infected wounds and dysentery. Chañar bark is an external tissue known for its medicinal properties, but its bioactive components were not reported yet. Objectives: To evaluate the antibacterial activity of polyphenolics (PG) and triterpenoids (TG) enriched fractions obtained from Geoffroea decorticans bark (CC) against Escherichia coli and Staphylococcus aureus. Methodology: CC grounded powder was washed with n-hexane and extracted with methanol. The extract was dried and fractionated by precipitation with acetone to separate TG from PG. The extracted material (EM) was determined for TG and PG. Antibacterial activity was evaluated against E. Coli (ATCC 25922) and S. aureus (ATCC 29213) by dot-blot bioautography and disk diffusion assay. Samples were assayed at 250 - 750 μg of EM, Ciprofloxacin was used as control. The plates were incubated at 37° C for 24 h. The bioactive fractions were analyzed by GC-MS. Results: PG (250 μg) was active against both strains. The inhibitory halo obtained against S. aureus was significant higher than for E. coli (p>0.05). TG did not showed activity against the assayed strains. GC-MS experiments performed for PG showed the presence of Dihydroquercetin, 3,4´-Dimethoxy-5,7,3´-trihydroxyflavone and protocatechic acid. Conclusions: CC exhibited antibacterial activity against Gram positive and Gram negative strains, which support ethnomedicinal use. This activity could be attributed to the phenolic compounds constituents.
Fil: Pastoriza, Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina
Fil: Iriarte, María Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina
Fil: Soberon, Jose Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina
Fil: Sgariglia, Melina Araceli. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales. Cátedra de Fitoquímica; Argentina
XXXVI Annual Scientific Meeting of the Tucuman Biology Association
Tafí Viejo
Argentina
Asociación de Biología de Tucumán
description Geoffroea decorticans, popularly known as "chañar", is traditionally used as anti-inflammatory, to treat infected wounds and dysentery. Chañar bark is an external tissue known for its medicinal properties, but its bioactive components were not reported yet. Objectives: To evaluate the antibacterial activity of polyphenolics (PG) and triterpenoids (TG) enriched fractions obtained from Geoffroea decorticans bark (CC) against Escherichia coli and Staphylococcus aureus. Methodology: CC grounded powder was washed with n-hexane and extracted with methanol. The extract was dried and fractionated by precipitation with acetone to separate TG from PG. The extracted material (EM) was determined for TG and PG. Antibacterial activity was evaluated against E. Coli (ATCC 25922) and S. aureus (ATCC 29213) by dot-blot bioautography and disk diffusion assay. Samples were assayed at 250 - 750 μg of EM, Ciprofloxacin was used as control. The plates were incubated at 37° C for 24 h. The bioactive fractions were analyzed by GC-MS. Results: PG (250 μg) was active against both strains. The inhibitory halo obtained against S. aureus was significant higher than for E. coli (p>0.05). TG did not showed activity against the assayed strains. GC-MS experiments performed for PG showed the presence of Dihydroquercetin, 3,4´-Dimethoxy-5,7,3´-trihydroxyflavone and protocatechic acid. Conclusions: CC exhibited antibacterial activity against Gram positive and Gram negative strains, which support ethnomedicinal use. This activity could be attributed to the phenolic compounds constituents.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/280956
Antibacterial activity of fractions obtained from stem bark of geoffroea decorticans tincture; XXXVI Annual Scientific Meeting of the Tucuman Biology Association; Tafí Viejo; Argentina; 2019; 25-25
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/280956
identifier_str_mv Antibacterial activity of fractions obtained from stem bark of geoffroea decorticans tincture; XXXVI Annual Scientific Meeting of the Tucuman Biology Association; Tafí Viejo; Argentina; 2019; 25-25
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
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application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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