Expression of melanocortin receptors mRNA, and direct effects of ACTH on steroids secretion in the bovine ovary

Autores
Amweg, Ayelen Noelia; Alfonso, Paredes; Salvetti, Natalia Raquel; Lara, Hernan E.; Ortega, Hugo Hector
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Melanocortin receptors (MCRs) are involved in physiological responses to ACTH, as well as to α-, β- and γ-melanocyte-stimulating hormone (α-, β- and γ-MSH). Their expression has previously been analyzed in various bovine tissues; however, there are apparently no reports regarding their localization in the ovaries. In the present study, the expression of MCR mRNA in various bovine ovarian structures was characterized with reverse transcription polymerase chain reaction (RT-PCR). Furthermore, whether ACTH affected follicular components by affecting steroid secretion in fragments of ovarian follicular wall of medium and large antral follicles cultured in serum free medium with 1, 10, and 100 nM ACTH, was also determined. Melanocortin receptors mRNA was localized in the theca cells of various follicular stages, whereas only MC3R mRNA was weakly evident in granulosa cells. Melanocortin receptors 1, 2, and 3 mRNA were present in the CL, whereas in stroma, only MC2R mRNA was expressed. There were significant increases in estradiol and cortisol concentrations in response to ACTH in medium follicles, as well as increased concentrations of testosterone and cortisol in large follicles. These results confirmed earlier reports in other species, and demonstrated that MCRs were present in bovine ovaries. Since ACTH induced steroid secretion from the ovary in vitro, we inferred that melanocortin peptides could be involved in regulatory mechanisms related to ovarian functions, e.g. ovulation, steroidogenesis, and luteal function.
Fil: Amweg, Ayelen Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Alfonso, Paredes. Universidad de Chile; Chile
Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Lara, Hernan E.. Universidad de Chile; Chile
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Materia
Ovary
Pomc
Melanocortin
Cattle
Acth
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/74057
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/74057
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression of melanocortin receptors mRNA, and direct effects of ACTH on steroids secretion in the bovine ovaryAmweg, Ayelen NoeliaAlfonso, ParedesSalvetti, Natalia RaquelLara, Hernan E.Ortega, Hugo HectorOvaryPomcMelanocortinCattleActhhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Melanocortin receptors (MCRs) are involved in physiological responses to ACTH, as well as to α-, β- and γ-melanocyte-stimulating hormone (α-, β- and γ-MSH). Their expression has previously been analyzed in various bovine tissues; however, there are apparently no reports regarding their localization in the ovaries. In the present study, the expression of MCR mRNA in various bovine ovarian structures was characterized with reverse transcription polymerase chain reaction (RT-PCR). Furthermore, whether ACTH affected follicular components by affecting steroid secretion in fragments of ovarian follicular wall of medium and large antral follicles cultured in serum free medium with 1, 10, and 100 nM ACTH, was also determined. Melanocortin receptors mRNA was localized in the theca cells of various follicular stages, whereas only MC3R mRNA was weakly evident in granulosa cells. Melanocortin receptors 1, 2, and 3 mRNA were present in the CL, whereas in stroma, only MC2R mRNA was expressed. There were significant increases in estradiol and cortisol concentrations in response to ACTH in medium follicles, as well as increased concentrations of testosterone and cortisol in large follicles. These results confirmed earlier reports in other species, and demonstrated that MCRs were present in bovine ovaries. Since ACTH induced steroid secretion from the ovary in vitro, we inferred that melanocortin peptides could be involved in regulatory mechanisms related to ovarian functions, e.g. ovulation, steroidogenesis, and luteal function.Fil: Amweg, Ayelen Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Alfonso, Paredes. Universidad de Chile; ChileFil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Lara, Hernan E.. Universidad de Chile; ChileFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaElsevier Science Inc2011-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/74057Amweg, Ayelen Noelia; Alfonso, Paredes; Salvetti, Natalia Raquel; Lara, Hernan E.; Ortega, Hugo Hector; Expression of melanocortin receptors mRNA, and direct effects of ACTH on steroids secretion in the bovine ovary; Elsevier Science Inc; Theriogenology; 75; 4; 9-2011; 628-6370093-691XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2010.10.003info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0093691X10005157info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:06Zoai:ri.conicet.gov.ar:11336/74057instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:07.025CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression of melanocortin receptors mRNA, and direct effects of ACTH on steroids secretion in the bovine ovary
title Expression of melanocortin receptors mRNA, and direct effects of ACTH on steroids secretion in the bovine ovary
spellingShingle Expression of melanocortin receptors mRNA, and direct effects of ACTH on steroids secretion in the bovine ovary
Amweg, Ayelen Noelia
Ovary
Pomc
Melanocortin
Cattle
Acth
title_short Expression of melanocortin receptors mRNA, and direct effects of ACTH on steroids secretion in the bovine ovary
title_full Expression of melanocortin receptors mRNA, and direct effects of ACTH on steroids secretion in the bovine ovary
title_fullStr Expression of melanocortin receptors mRNA, and direct effects of ACTH on steroids secretion in the bovine ovary
title_full_unstemmed Expression of melanocortin receptors mRNA, and direct effects of ACTH on steroids secretion in the bovine ovary
title_sort Expression of melanocortin receptors mRNA, and direct effects of ACTH on steroids secretion in the bovine ovary
dc.creator.none.fl_str_mv Amweg, Ayelen Noelia
Alfonso, Paredes
Salvetti, Natalia Raquel
Lara, Hernan E.
Ortega, Hugo Hector
author Amweg, Ayelen Noelia
author_facet Amweg, Ayelen Noelia
Alfonso, Paredes
Salvetti, Natalia Raquel
Lara, Hernan E.
Ortega, Hugo Hector
author_role author
author2 Alfonso, Paredes
Salvetti, Natalia Raquel
Lara, Hernan E.
Ortega, Hugo Hector
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ovary
Pomc
Melanocortin
Cattle
Acth
topic Ovary
Pomc
Melanocortin
Cattle
Acth
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Melanocortin receptors (MCRs) are involved in physiological responses to ACTH, as well as to α-, β- and γ-melanocyte-stimulating hormone (α-, β- and γ-MSH). Their expression has previously been analyzed in various bovine tissues; however, there are apparently no reports regarding their localization in the ovaries. In the present study, the expression of MCR mRNA in various bovine ovarian structures was characterized with reverse transcription polymerase chain reaction (RT-PCR). Furthermore, whether ACTH affected follicular components by affecting steroid secretion in fragments of ovarian follicular wall of medium and large antral follicles cultured in serum free medium with 1, 10, and 100 nM ACTH, was also determined. Melanocortin receptors mRNA was localized in the theca cells of various follicular stages, whereas only MC3R mRNA was weakly evident in granulosa cells. Melanocortin receptors 1, 2, and 3 mRNA were present in the CL, whereas in stroma, only MC2R mRNA was expressed. There were significant increases in estradiol and cortisol concentrations in response to ACTH in medium follicles, as well as increased concentrations of testosterone and cortisol in large follicles. These results confirmed earlier reports in other species, and demonstrated that MCRs were present in bovine ovaries. Since ACTH induced steroid secretion from the ovary in vitro, we inferred that melanocortin peptides could be involved in regulatory mechanisms related to ovarian functions, e.g. ovulation, steroidogenesis, and luteal function.
Fil: Amweg, Ayelen Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Alfonso, Paredes. Universidad de Chile; Chile
Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Lara, Hernan E.. Universidad de Chile; Chile
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
description Melanocortin receptors (MCRs) are involved in physiological responses to ACTH, as well as to α-, β- and γ-melanocyte-stimulating hormone (α-, β- and γ-MSH). Their expression has previously been analyzed in various bovine tissues; however, there are apparently no reports regarding their localization in the ovaries. In the present study, the expression of MCR mRNA in various bovine ovarian structures was characterized with reverse transcription polymerase chain reaction (RT-PCR). Furthermore, whether ACTH affected follicular components by affecting steroid secretion in fragments of ovarian follicular wall of medium and large antral follicles cultured in serum free medium with 1, 10, and 100 nM ACTH, was also determined. Melanocortin receptors mRNA was localized in the theca cells of various follicular stages, whereas only MC3R mRNA was weakly evident in granulosa cells. Melanocortin receptors 1, 2, and 3 mRNA were present in the CL, whereas in stroma, only MC2R mRNA was expressed. There were significant increases in estradiol and cortisol concentrations in response to ACTH in medium follicles, as well as increased concentrations of testosterone and cortisol in large follicles. These results confirmed earlier reports in other species, and demonstrated that MCRs were present in bovine ovaries. Since ACTH induced steroid secretion from the ovary in vitro, we inferred that melanocortin peptides could be involved in regulatory mechanisms related to ovarian functions, e.g. ovulation, steroidogenesis, and luteal function.
publishDate 2011
dc.date.none.fl_str_mv 2011-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/74057
Amweg, Ayelen Noelia; Alfonso, Paredes; Salvetti, Natalia Raquel; Lara, Hernan E.; Ortega, Hugo Hector; Expression of melanocortin receptors mRNA, and direct effects of ACTH on steroids secretion in the bovine ovary; Elsevier Science Inc; Theriogenology; 75; 4; 9-2011; 628-637
0093-691X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/74057
identifier_str_mv Amweg, Ayelen Noelia; Alfonso, Paredes; Salvetti, Natalia Raquel; Lara, Hernan E.; Ortega, Hugo Hector; Expression of melanocortin receptors mRNA, and direct effects of ACTH on steroids secretion in the bovine ovary; Elsevier Science Inc; Theriogenology; 75; 4; 9-2011; 628-637
0093-691X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.theriogenology.2010.10.003
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0093691X10005157
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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