In vitro human umbilical vein endothelial cells response to ionic dissolution products from lithium-containing 45S5 bioactive glass
- Autores
- Haro Durand, Luis Alberto; Vargas, Gabriela Elizabet; Vera Mesones, Rosa; Baldi, Alberto; Zago, María Paola; Fanovich, Maria Alejandra; Boccaccini, Aldo R.; Gorustovich Alonso, Alejandro Adrian
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Since lithium (Li+) plays roles in angiogenesis, the localized and controlled release of Li+ ions from bioactive glasses (BGs) represents a promising alternative therapy for the regeneration and repair of tissues with a high degree of vascularization. Here, microparticles from a base 45S5 BG composition containing (wt %) 45% SiO2, 24.5% Na2O, 24.5% CaO, and 6% P2O5, in which Na2O was partially substituted by 5% Li2O (45S5.5Li), were obtained. The results demonstrate that human umbilical vein endothelial cells (HUVECs) have greater migratory and proliferative response and ability to form tubules in vitro after stimulation with the ionic dissolution products (IDPs) of the 45S5.5Li BG. The results also show the activation of the canonical Wnt/β-catenin pathway and the increase in expression of proangiogenic cytokines insulin like growth factor 1 (IGF1) and transforming growth factor beta (TGFβ). We conclude that the IDPs of 45S5.5Li BG would act as useful inorganic agents to improve tissue repair and regeneration, ultimately stimulating HUVECs behavior in the absence of exogenous growth factors.
Fil: Haro Durand, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Vargas, Gabriela Elizabet. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vera Mesones, Rosa. Universidad Nacional de Salta; Argentina
Fil: Baldi, Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Zago, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Fanovich, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; Alemania
Fil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina - Materia
-
BIOACTIVE GLASS
ENDOTHELIAL CELL
LITHIUM - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/64400
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oai:ri.conicet.gov.ar:11336/64400 |
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In vitro human umbilical vein endothelial cells response to ionic dissolution products from lithium-containing 45S5 bioactive glassHaro Durand, Luis AlbertoVargas, Gabriela ElizabetVera Mesones, RosaBaldi, AlbertoZago, María PaolaFanovich, Maria AlejandraBoccaccini, Aldo R.Gorustovich Alonso, Alejandro AdrianBIOACTIVE GLASSENDOTHELIAL CELLLITHIUMhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Since lithium (Li+) plays roles in angiogenesis, the localized and controlled release of Li+ ions from bioactive glasses (BGs) represents a promising alternative therapy for the regeneration and repair of tissues with a high degree of vascularization. Here, microparticles from a base 45S5 BG composition containing (wt %) 45% SiO2, 24.5% Na2O, 24.5% CaO, and 6% P2O5, in which Na2O was partially substituted by 5% Li2O (45S5.5Li), were obtained. The results demonstrate that human umbilical vein endothelial cells (HUVECs) have greater migratory and proliferative response and ability to form tubules in vitro after stimulation with the ionic dissolution products (IDPs) of the 45S5.5Li BG. The results also show the activation of the canonical Wnt/β-catenin pathway and the increase in expression of proangiogenic cytokines insulin like growth factor 1 (IGF1) and transforming growth factor beta (TGFβ). We conclude that the IDPs of 45S5.5Li BG would act as useful inorganic agents to improve tissue repair and regeneration, ultimately stimulating HUVECs behavior in the absence of exogenous growth factors.Fil: Haro Durand, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Vargas, Gabriela Elizabet. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vera Mesones, Rosa. Universidad Nacional de Salta; ArgentinaFil: Baldi, Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Zago, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Fanovich, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; AlemaniaFil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; ArgentinaMDPI2017-07-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64400Haro Durand, Luis Alberto; Vargas, Gabriela Elizabet; Vera Mesones, Rosa; Baldi, Alberto; Zago, María Paola; et al.; In vitro human umbilical vein endothelial cells response to ionic dissolution products from lithium-containing 45S5 bioactive glass; MDPI; Materials; 10; 7; 3-7-2017; 7401996-1944CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1996-1944/10/7/740info:eu-repo/semantics/altIdentifier/doi/10.3390/ma10070740info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:40:04Zoai:ri.conicet.gov.ar:11336/64400instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:40:04.658CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
In vitro human umbilical vein endothelial cells response to ionic dissolution products from lithium-containing 45S5 bioactive glass |
title |
In vitro human umbilical vein endothelial cells response to ionic dissolution products from lithium-containing 45S5 bioactive glass |
spellingShingle |
In vitro human umbilical vein endothelial cells response to ionic dissolution products from lithium-containing 45S5 bioactive glass Haro Durand, Luis Alberto BIOACTIVE GLASS ENDOTHELIAL CELL LITHIUM |
title_short |
In vitro human umbilical vein endothelial cells response to ionic dissolution products from lithium-containing 45S5 bioactive glass |
title_full |
In vitro human umbilical vein endothelial cells response to ionic dissolution products from lithium-containing 45S5 bioactive glass |
title_fullStr |
In vitro human umbilical vein endothelial cells response to ionic dissolution products from lithium-containing 45S5 bioactive glass |
title_full_unstemmed |
In vitro human umbilical vein endothelial cells response to ionic dissolution products from lithium-containing 45S5 bioactive glass |
title_sort |
In vitro human umbilical vein endothelial cells response to ionic dissolution products from lithium-containing 45S5 bioactive glass |
dc.creator.none.fl_str_mv |
Haro Durand, Luis Alberto Vargas, Gabriela Elizabet Vera Mesones, Rosa Baldi, Alberto Zago, María Paola Fanovich, Maria Alejandra Boccaccini, Aldo R. Gorustovich Alonso, Alejandro Adrian |
author |
Haro Durand, Luis Alberto |
author_facet |
Haro Durand, Luis Alberto Vargas, Gabriela Elizabet Vera Mesones, Rosa Baldi, Alberto Zago, María Paola Fanovich, Maria Alejandra Boccaccini, Aldo R. Gorustovich Alonso, Alejandro Adrian |
author_role |
author |
author2 |
Vargas, Gabriela Elizabet Vera Mesones, Rosa Baldi, Alberto Zago, María Paola Fanovich, Maria Alejandra Boccaccini, Aldo R. Gorustovich Alonso, Alejandro Adrian |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
BIOACTIVE GLASS ENDOTHELIAL CELL LITHIUM |
topic |
BIOACTIVE GLASS ENDOTHELIAL CELL LITHIUM |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.4 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Since lithium (Li+) plays roles in angiogenesis, the localized and controlled release of Li+ ions from bioactive glasses (BGs) represents a promising alternative therapy for the regeneration and repair of tissues with a high degree of vascularization. Here, microparticles from a base 45S5 BG composition containing (wt %) 45% SiO2, 24.5% Na2O, 24.5% CaO, and 6% P2O5, in which Na2O was partially substituted by 5% Li2O (45S5.5Li), were obtained. The results demonstrate that human umbilical vein endothelial cells (HUVECs) have greater migratory and proliferative response and ability to form tubules in vitro after stimulation with the ionic dissolution products (IDPs) of the 45S5.5Li BG. The results also show the activation of the canonical Wnt/β-catenin pathway and the increase in expression of proangiogenic cytokines insulin like growth factor 1 (IGF1) and transforming growth factor beta (TGFβ). We conclude that the IDPs of 45S5.5Li BG would act as useful inorganic agents to improve tissue repair and regeneration, ultimately stimulating HUVECs behavior in the absence of exogenous growth factors. Fil: Haro Durand, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Vargas, Gabriela Elizabet. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vera Mesones, Rosa. Universidad Nacional de Salta; Argentina Fil: Baldi, Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Zago, María Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina Fil: Fanovich, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina Fil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; Alemania Fil: Gorustovich Alonso, Alejandro Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long". Universidad de Buenos Aires. Facultad de Ingeniería. Instituto de Tecnologías y Ciencias de la Ingeniería "Hilario Fernández Long"; Argentina |
description |
Since lithium (Li+) plays roles in angiogenesis, the localized and controlled release of Li+ ions from bioactive glasses (BGs) represents a promising alternative therapy for the regeneration and repair of tissues with a high degree of vascularization. Here, microparticles from a base 45S5 BG composition containing (wt %) 45% SiO2, 24.5% Na2O, 24.5% CaO, and 6% P2O5, in which Na2O was partially substituted by 5% Li2O (45S5.5Li), were obtained. The results demonstrate that human umbilical vein endothelial cells (HUVECs) have greater migratory and proliferative response and ability to form tubules in vitro after stimulation with the ionic dissolution products (IDPs) of the 45S5.5Li BG. The results also show the activation of the canonical Wnt/β-catenin pathway and the increase in expression of proangiogenic cytokines insulin like growth factor 1 (IGF1) and transforming growth factor beta (TGFβ). We conclude that the IDPs of 45S5.5Li BG would act as useful inorganic agents to improve tissue repair and regeneration, ultimately stimulating HUVECs behavior in the absence of exogenous growth factors. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/64400 Haro Durand, Luis Alberto; Vargas, Gabriela Elizabet; Vera Mesones, Rosa; Baldi, Alberto; Zago, María Paola; et al.; In vitro human umbilical vein endothelial cells response to ionic dissolution products from lithium-containing 45S5 bioactive glass; MDPI; Materials; 10; 7; 3-7-2017; 740 1996-1944 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/64400 |
identifier_str_mv |
Haro Durand, Luis Alberto; Vargas, Gabriela Elizabet; Vera Mesones, Rosa; Baldi, Alberto; Zago, María Paola; et al.; In vitro human umbilical vein endothelial cells response to ionic dissolution products from lithium-containing 45S5 bioactive glass; MDPI; Materials; 10; 7; 3-7-2017; 740 1996-1944 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1996-1944/10/7/740 info:eu-repo/semantics/altIdentifier/doi/10.3390/ma10070740 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
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MDPI |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613267147718656 |
score |
13.070432 |