Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray

Autores
Fernandino, Juan Ignacio; Popesku, J. T.; Paul Prasanth, B.; Xiong, H.; Hattori, R.S.; Oura, M.; Strüssmann, C. A.; Somoza, Gustavo Manuel; Matsuda, M.; Nagahama, Y.; Trudeau, V. L.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The process of morphological development of a differentiated gonad from an undifferentiated primordium is a very important step of gonadogenesis. Studies on sexually dimorphic gene expression are important to increase our understanding of this process and to investigate how environmental factors such as temperature can regulate gonadal development. The aim of this study was to identify putative genes involved in sex differentiation in pejerrey (Odontesthes bonariensis) reared at male- and female-producing temperatures (MPT and FPT, respectively) using a microarray heterologous from the medaka (Oryzias latipes), a closely phylogenetic species. Genes related to numerous processes presented higher expression at MPT, including those involved in muscular contraction, metabolic pathways, developmental processes, and reproduction. Genes induced by FPT were classified under the gene ontology terms of response to stimulus, transport and proteolysis. From genes selected for validation, at MPT ndrg3 expression was observed in the somatic cells, whereas pen-2 was detected in germ cells in the caudal portion of the gonads, where no apoptotic signals were observed. Finally, hsp90 was highly expressed in somatic cells of the gonads at the FPT. The results suggest that the interplay of pro-apoptotic and anti-apoptotic genes is important during the masculinization process and for the prevention of sterility following exposure to warm temperatures.
Fil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Popesku, J. T.. University of Ottawa. Faculty of Science. Centre for Advanced Research in Environmental Genomics; Canadá
Fil: Paul Prasanth, B.. Tokyo University of Marine Science and Technology; Japón
Fil: Xiong, H.. University of Ottawa. Faculty of Science. Centre for Advanced Research in Environmental Genomics; Canadá
Fil: Hattori, R.S.. Tokyo University of Marine Science and Technology; Japón
Fil: Oura, M.. Tokyo University of Marine Science and Technology; Japón
Fil: Strüssmann, C. A.. Tokyo University of Marine Science and Technology; Japón
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Matsuda, M.. Tokyo University of Marine Science and Technology; Japón
Fil: Nagahama, Y.. Tokyo University of Marine Science and Technology; Japón
Fil: Trudeau, V. L.. University of Ottawa. Faculty of Science. Centre for Advanced Research in Environmental Genomics; Canadá
Materia
APOPTOSIS
GONADOGENESIS
MICROARRAY
PEJERREY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/108682
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/108682
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarrayFernandino, Juan IgnacioPopesku, J. T.Paul Prasanth, B.Xiong, H.Hattori, R.S.Oura, M.Strüssmann, C. A.Somoza, Gustavo ManuelMatsuda, M.Nagahama, Y.Trudeau, V. L.APOPTOSISGONADOGENESISMICROARRAYPEJERREYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The process of morphological development of a differentiated gonad from an undifferentiated primordium is a very important step of gonadogenesis. Studies on sexually dimorphic gene expression are important to increase our understanding of this process and to investigate how environmental factors such as temperature can regulate gonadal development. The aim of this study was to identify putative genes involved in sex differentiation in pejerrey (Odontesthes bonariensis) reared at male- and female-producing temperatures (MPT and FPT, respectively) using a microarray heterologous from the medaka (Oryzias latipes), a closely phylogenetic species. Genes related to numerous processes presented higher expression at MPT, including those involved in muscular contraction, metabolic pathways, developmental processes, and reproduction. Genes induced by FPT were classified under the gene ontology terms of response to stimulus, transport and proteolysis. From genes selected for validation, at MPT ndrg3 expression was observed in the somatic cells, whereas pen-2 was detected in germ cells in the caudal portion of the gonads, where no apoptotic signals were observed. Finally, hsp90 was highly expressed in somatic cells of the gonads at the FPT. The results suggest that the interplay of pro-apoptotic and anti-apoptotic genes is important during the masculinization process and for the prevention of sterility following exposure to warm temperatures.Fil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Popesku, J. T.. University of Ottawa. Faculty of Science. Centre for Advanced Research in Environmental Genomics; CanadáFil: Paul Prasanth, B.. Tokyo University of Marine Science and Technology; JapónFil: Xiong, H.. University of Ottawa. Faculty of Science. Centre for Advanced Research in Environmental Genomics; CanadáFil: Hattori, R.S.. Tokyo University of Marine Science and Technology; JapónFil: Oura, M.. Tokyo University of Marine Science and Technology; JapónFil: Strüssmann, C. A.. Tokyo University of Marine Science and Technology; JapónFil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Matsuda, M.. Tokyo University of Marine Science and Technology; JapónFil: Nagahama, Y.. Tokyo University of Marine Science and Technology; JapónFil: Trudeau, V. L.. University of Ottawa. Faculty of Science. Centre for Advanced Research in Environmental Genomics; CanadáKarger2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/108682Fernandino, Juan Ignacio; Popesku, J. T.; Paul Prasanth, B.; Xiong, H.; Hattori, R.S.; et al.; Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray; Karger; Sexual Development; 5; 2; 3-2011; 89-1011661-5425CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/324423info:eu-repo/semantics/altIdentifier/doi/10.1159/000324423info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:51:29Zoai:ri.conicet.gov.ar:11336/108682instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:51:29.979CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray
title Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray
spellingShingle Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray
Fernandino, Juan Ignacio
APOPTOSIS
GONADOGENESIS
MICROARRAY
PEJERREY
title_short Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray
title_full Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray
title_fullStr Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray
title_full_unstemmed Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray
title_sort Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray
dc.creator.none.fl_str_mv Fernandino, Juan Ignacio
Popesku, J. T.
Paul Prasanth, B.
Xiong, H.
Hattori, R.S.
Oura, M.
Strüssmann, C. A.
Somoza, Gustavo Manuel
Matsuda, M.
Nagahama, Y.
Trudeau, V. L.
author Fernandino, Juan Ignacio
author_facet Fernandino, Juan Ignacio
Popesku, J. T.
Paul Prasanth, B.
Xiong, H.
Hattori, R.S.
Oura, M.
Strüssmann, C. A.
Somoza, Gustavo Manuel
Matsuda, M.
Nagahama, Y.
Trudeau, V. L.
author_role author
author2 Popesku, J. T.
Paul Prasanth, B.
Xiong, H.
Hattori, R.S.
Oura, M.
Strüssmann, C. A.
Somoza, Gustavo Manuel
Matsuda, M.
Nagahama, Y.
Trudeau, V. L.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
GONADOGENESIS
MICROARRAY
PEJERREY
topic APOPTOSIS
GONADOGENESIS
MICROARRAY
PEJERREY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The process of morphological development of a differentiated gonad from an undifferentiated primordium is a very important step of gonadogenesis. Studies on sexually dimorphic gene expression are important to increase our understanding of this process and to investigate how environmental factors such as temperature can regulate gonadal development. The aim of this study was to identify putative genes involved in sex differentiation in pejerrey (Odontesthes bonariensis) reared at male- and female-producing temperatures (MPT and FPT, respectively) using a microarray heterologous from the medaka (Oryzias latipes), a closely phylogenetic species. Genes related to numerous processes presented higher expression at MPT, including those involved in muscular contraction, metabolic pathways, developmental processes, and reproduction. Genes induced by FPT were classified under the gene ontology terms of response to stimulus, transport and proteolysis. From genes selected for validation, at MPT ndrg3 expression was observed in the somatic cells, whereas pen-2 was detected in germ cells in the caudal portion of the gonads, where no apoptotic signals were observed. Finally, hsp90 was highly expressed in somatic cells of the gonads at the FPT. The results suggest that the interplay of pro-apoptotic and anti-apoptotic genes is important during the masculinization process and for the prevention of sterility following exposure to warm temperatures.
Fil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Popesku, J. T.. University of Ottawa. Faculty of Science. Centre for Advanced Research in Environmental Genomics; Canadá
Fil: Paul Prasanth, B.. Tokyo University of Marine Science and Technology; Japón
Fil: Xiong, H.. University of Ottawa. Faculty of Science. Centre for Advanced Research in Environmental Genomics; Canadá
Fil: Hattori, R.S.. Tokyo University of Marine Science and Technology; Japón
Fil: Oura, M.. Tokyo University of Marine Science and Technology; Japón
Fil: Strüssmann, C. A.. Tokyo University of Marine Science and Technology; Japón
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Matsuda, M.. Tokyo University of Marine Science and Technology; Japón
Fil: Nagahama, Y.. Tokyo University of Marine Science and Technology; Japón
Fil: Trudeau, V. L.. University of Ottawa. Faculty of Science. Centre for Advanced Research in Environmental Genomics; Canadá
description The process of morphological development of a differentiated gonad from an undifferentiated primordium is a very important step of gonadogenesis. Studies on sexually dimorphic gene expression are important to increase our understanding of this process and to investigate how environmental factors such as temperature can regulate gonadal development. The aim of this study was to identify putative genes involved in sex differentiation in pejerrey (Odontesthes bonariensis) reared at male- and female-producing temperatures (MPT and FPT, respectively) using a microarray heterologous from the medaka (Oryzias latipes), a closely phylogenetic species. Genes related to numerous processes presented higher expression at MPT, including those involved in muscular contraction, metabolic pathways, developmental processes, and reproduction. Genes induced by FPT were classified under the gene ontology terms of response to stimulus, transport and proteolysis. From genes selected for validation, at MPT ndrg3 expression was observed in the somatic cells, whereas pen-2 was detected in germ cells in the caudal portion of the gonads, where no apoptotic signals were observed. Finally, hsp90 was highly expressed in somatic cells of the gonads at the FPT. The results suggest that the interplay of pro-apoptotic and anti-apoptotic genes is important during the masculinization process and for the prevention of sterility following exposure to warm temperatures.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/108682
Fernandino, Juan Ignacio; Popesku, J. T.; Paul Prasanth, B.; Xiong, H.; Hattori, R.S.; et al.; Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray; Karger; Sexual Development; 5; 2; 3-2011; 89-101
1661-5425
CONICET Digital
CONICET
url http://hdl.handle.net/11336/108682
identifier_str_mv Fernandino, Juan Ignacio; Popesku, J. T.; Paul Prasanth, B.; Xiong, H.; Hattori, R.S.; et al.; Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray; Karger; Sexual Development; 5; 2; 3-2011; 89-101
1661-5425
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/324423
info:eu-repo/semantics/altIdentifier/doi/10.1159/000324423
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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