Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis
- Autores
- Lichtenstein, Gabriel; Elisio, Mariano; Miranda, Leandro Andres
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The pejerrey, Odontesthes bonariensis, is being considered for aquaculture due to its high demand and market price. Reproduction and larviculture studies have demonstrated the feasibility of massive fingerling production, and techniques that prolong life and increase gamete viability can assist in the culture development of this species. In this regard, the main objective of this study was to develop freezing protocols for pejerrey sperm. For this purpose, two extenders: Ex1, a modified Mounib solution (127 mM NaHCO3, 159 mM sucrose, 0.025 g/ml reduced glutathione; pH: 8; osmolality 400 mOsm/kg) and Ex2, a saline based solution (250 mM NaHCO3, 100 mM trehalose; pH: 8; osmolality 450 mOsm/kg) were developed. Dimethyl sulfoxide (DMSO) and ethylene glycol, (EG) were added at 10% as cryoprotectants and two types of containers were used: cryovials (1 ml of volume) and French straws (0.250 ml). Cryopreservation was made without equilibration time using dry ice or liquid nitrogen. The results obtained by freezing pejerrey semen with dry ice showed that all the combinations tested were suitable because high motility indexes (among 4 or 5) and good fertility percentages (between 46% and 56%) were obtained after thawing. However, the fertilization percentage obtained with control semen was significantly higher (80%). No significant statistical differences were observed on the fertilization percentages between experimental combinations or the containers used. In the case of liquid nitrogen as freezing method, it was possible to obtain for all the combinations motility indexes among 3 or 4 after thawing. In the case of fertilization trials, similar percentages (around 80%) were found for control semen and for the majority of cryopreserved samples. However, statistically lower results (among 44%–60%) were found for Ex1DMSO (cryotubes) samples and for Ex1EG samples in both containers. In summary, the feasibility to cryopreserve pejerrey sperm was demonstrated for the first time using simple and practical protocols.
Fil: Lichtenstein, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Elisio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Miranda, Leandro Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina - Materia
-
Cryopreservation
Sperm
Pejerrey
Reproduction - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/243596
Ver los metadatos del registro completo
id |
CONICETDig_3d44ac5a77d602513e661a269b1ecf74 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/243596 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensisLichtenstein, GabrielElisio, MarianoMiranda, Leandro AndresCryopreservationSpermPejerreyReproductionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The pejerrey, Odontesthes bonariensis, is being considered for aquaculture due to its high demand and market price. Reproduction and larviculture studies have demonstrated the feasibility of massive fingerling production, and techniques that prolong life and increase gamete viability can assist in the culture development of this species. In this regard, the main objective of this study was to develop freezing protocols for pejerrey sperm. For this purpose, two extenders: Ex1, a modified Mounib solution (127 mM NaHCO3, 159 mM sucrose, 0.025 g/ml reduced glutathione; pH: 8; osmolality 400 mOsm/kg) and Ex2, a saline based solution (250 mM NaHCO3, 100 mM trehalose; pH: 8; osmolality 450 mOsm/kg) were developed. Dimethyl sulfoxide (DMSO) and ethylene glycol, (EG) were added at 10% as cryoprotectants and two types of containers were used: cryovials (1 ml of volume) and French straws (0.250 ml). Cryopreservation was made without equilibration time using dry ice or liquid nitrogen. The results obtained by freezing pejerrey semen with dry ice showed that all the combinations tested were suitable because high motility indexes (among 4 or 5) and good fertility percentages (between 46% and 56%) were obtained after thawing. However, the fertilization percentage obtained with control semen was significantly higher (80%). No significant statistical differences were observed on the fertilization percentages between experimental combinations or the containers used. In the case of liquid nitrogen as freezing method, it was possible to obtain for all the combinations motility indexes among 3 or 4 after thawing. In the case of fertilization trials, similar percentages (around 80%) were found for control semen and for the majority of cryopreserved samples. However, statistically lower results (among 44%–60%) were found for Ex1DMSO (cryotubes) samples and for Ex1EG samples in both containers. In summary, the feasibility to cryopreserve pejerrey sperm was demonstrated for the first time using simple and practical protocols.Fil: Lichtenstein, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Elisio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Miranda, Leandro Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaElsevier Science2010-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243596Lichtenstein, Gabriel; Elisio, Mariano; Miranda, Leandro Andres; Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis; Elsevier Science; Aquaculture; 306; 1-4; 8-2010; 357-3610044-8486CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S004484861000325Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2010.05.016info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:44Zoai:ri.conicet.gov.ar:11336/243596instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:44.423CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis |
title |
Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis |
spellingShingle |
Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis Lichtenstein, Gabriel Cryopreservation Sperm Pejerrey Reproduction |
title_short |
Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis |
title_full |
Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis |
title_fullStr |
Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis |
title_full_unstemmed |
Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis |
title_sort |
Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis |
dc.creator.none.fl_str_mv |
Lichtenstein, Gabriel Elisio, Mariano Miranda, Leandro Andres |
author |
Lichtenstein, Gabriel |
author_facet |
Lichtenstein, Gabriel Elisio, Mariano Miranda, Leandro Andres |
author_role |
author |
author2 |
Elisio, Mariano Miranda, Leandro Andres |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Cryopreservation Sperm Pejerrey Reproduction |
topic |
Cryopreservation Sperm Pejerrey Reproduction |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The pejerrey, Odontesthes bonariensis, is being considered for aquaculture due to its high demand and market price. Reproduction and larviculture studies have demonstrated the feasibility of massive fingerling production, and techniques that prolong life and increase gamete viability can assist in the culture development of this species. In this regard, the main objective of this study was to develop freezing protocols for pejerrey sperm. For this purpose, two extenders: Ex1, a modified Mounib solution (127 mM NaHCO3, 159 mM sucrose, 0.025 g/ml reduced glutathione; pH: 8; osmolality 400 mOsm/kg) and Ex2, a saline based solution (250 mM NaHCO3, 100 mM trehalose; pH: 8; osmolality 450 mOsm/kg) were developed. Dimethyl sulfoxide (DMSO) and ethylene glycol, (EG) were added at 10% as cryoprotectants and two types of containers were used: cryovials (1 ml of volume) and French straws (0.250 ml). Cryopreservation was made without equilibration time using dry ice or liquid nitrogen. The results obtained by freezing pejerrey semen with dry ice showed that all the combinations tested were suitable because high motility indexes (among 4 or 5) and good fertility percentages (between 46% and 56%) were obtained after thawing. However, the fertilization percentage obtained with control semen was significantly higher (80%). No significant statistical differences were observed on the fertilization percentages between experimental combinations or the containers used. In the case of liquid nitrogen as freezing method, it was possible to obtain for all the combinations motility indexes among 3 or 4 after thawing. In the case of fertilization trials, similar percentages (around 80%) were found for control semen and for the majority of cryopreserved samples. However, statistically lower results (among 44%–60%) were found for Ex1DMSO (cryotubes) samples and for Ex1EG samples in both containers. In summary, the feasibility to cryopreserve pejerrey sperm was demonstrated for the first time using simple and practical protocols. Fil: Lichtenstein, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Elisio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Miranda, Leandro Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina |
description |
The pejerrey, Odontesthes bonariensis, is being considered for aquaculture due to its high demand and market price. Reproduction and larviculture studies have demonstrated the feasibility of massive fingerling production, and techniques that prolong life and increase gamete viability can assist in the culture development of this species. In this regard, the main objective of this study was to develop freezing protocols for pejerrey sperm. For this purpose, two extenders: Ex1, a modified Mounib solution (127 mM NaHCO3, 159 mM sucrose, 0.025 g/ml reduced glutathione; pH: 8; osmolality 400 mOsm/kg) and Ex2, a saline based solution (250 mM NaHCO3, 100 mM trehalose; pH: 8; osmolality 450 mOsm/kg) were developed. Dimethyl sulfoxide (DMSO) and ethylene glycol, (EG) were added at 10% as cryoprotectants and two types of containers were used: cryovials (1 ml of volume) and French straws (0.250 ml). Cryopreservation was made without equilibration time using dry ice or liquid nitrogen. The results obtained by freezing pejerrey semen with dry ice showed that all the combinations tested were suitable because high motility indexes (among 4 or 5) and good fertility percentages (between 46% and 56%) were obtained after thawing. However, the fertilization percentage obtained with control semen was significantly higher (80%). No significant statistical differences were observed on the fertilization percentages between experimental combinations or the containers used. In the case of liquid nitrogen as freezing method, it was possible to obtain for all the combinations motility indexes among 3 or 4 after thawing. In the case of fertilization trials, similar percentages (around 80%) were found for control semen and for the majority of cryopreserved samples. However, statistically lower results (among 44%–60%) were found for Ex1DMSO (cryotubes) samples and for Ex1EG samples in both containers. In summary, the feasibility to cryopreserve pejerrey sperm was demonstrated for the first time using simple and practical protocols. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/243596 Lichtenstein, Gabriel; Elisio, Mariano; Miranda, Leandro Andres; Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis; Elsevier Science; Aquaculture; 306; 1-4; 8-2010; 357-361 0044-8486 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/243596 |
identifier_str_mv |
Lichtenstein, Gabriel; Elisio, Mariano; Miranda, Leandro Andres; Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis; Elsevier Science; Aquaculture; 306; 1-4; 8-2010; 357-361 0044-8486 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S004484861000325X info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2010.05.016 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842268619030921216 |
score |
13.13397 |