Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis

Autores
Lichtenstein, Gabriel; Elisio, Mariano; Miranda, Leandro Andres
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The pejerrey, Odontesthes bonariensis, is being considered for aquaculture due to its high demand and market price. Reproduction and larviculture studies have demonstrated the feasibility of massive fingerling production, and techniques that prolong life and increase gamete viability can assist in the culture development of this species. In this regard, the main objective of this study was to develop freezing protocols for pejerrey sperm. For this purpose, two extenders: Ex1, a modified Mounib solution (127 mM NaHCO3, 159 mM sucrose, 0.025 g/ml reduced glutathione; pH: 8; osmolality 400 mOsm/kg) and Ex2, a saline based solution (250 mM NaHCO3, 100 mM trehalose; pH: 8; osmolality 450 mOsm/kg) were developed. Dimethyl sulfoxide (DMSO) and ethylene glycol, (EG) were added at 10% as cryoprotectants and two types of containers were used: cryovials (1 ml of volume) and French straws (0.250 ml). Cryopreservation was made without equilibration time using dry ice or liquid nitrogen. The results obtained by freezing pejerrey semen with dry ice showed that all the combinations tested were suitable because high motility indexes (among 4 or 5) and good fertility percentages (between 46% and 56%) were obtained after thawing. However, the fertilization percentage obtained with control semen was significantly higher (80%). No significant statistical differences were observed on the fertilization percentages between experimental combinations or the containers used. In the case of liquid nitrogen as freezing method, it was possible to obtain for all the combinations motility indexes among 3 or 4 after thawing. In the case of fertilization trials, similar percentages (around 80%) were found for control semen and for the majority of cryopreserved samples. However, statistically lower results (among 44%–60%) were found for Ex1DMSO (cryotubes) samples and for Ex1EG samples in both containers. In summary, the feasibility to cryopreserve pejerrey sperm was demonstrated for the first time using simple and practical protocols.
Fil: Lichtenstein, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Elisio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Miranda, Leandro Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Materia
Cryopreservation
Sperm
Pejerrey
Reproduction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243596
Acceder
id CONICETDig_3d44ac5a77d602513e661a269b1ecf74
oai_identifier_str oai:ri.conicet.gov.ar:11336/243596
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensisLichtenstein, GabrielElisio, MarianoMiranda, Leandro AndresCryopreservationSpermPejerreyReproductionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The pejerrey, Odontesthes bonariensis, is being considered for aquaculture due to its high demand and market price. Reproduction and larviculture studies have demonstrated the feasibility of massive fingerling production, and techniques that prolong life and increase gamete viability can assist in the culture development of this species. In this regard, the main objective of this study was to develop freezing protocols for pejerrey sperm. For this purpose, two extenders: Ex1, a modified Mounib solution (127 mM NaHCO3, 159 mM sucrose, 0.025 g/ml reduced glutathione; pH: 8; osmolality 400 mOsm/kg) and Ex2, a saline based solution (250 mM NaHCO3, 100 mM trehalose; pH: 8; osmolality 450 mOsm/kg) were developed. Dimethyl sulfoxide (DMSO) and ethylene glycol, (EG) were added at 10% as cryoprotectants and two types of containers were used: cryovials (1 ml of volume) and French straws (0.250 ml). Cryopreservation was made without equilibration time using dry ice or liquid nitrogen. The results obtained by freezing pejerrey semen with dry ice showed that all the combinations tested were suitable because high motility indexes (among 4 or 5) and good fertility percentages (between 46% and 56%) were obtained after thawing. However, the fertilization percentage obtained with control semen was significantly higher (80%). No significant statistical differences were observed on the fertilization percentages between experimental combinations or the containers used. In the case of liquid nitrogen as freezing method, it was possible to obtain for all the combinations motility indexes among 3 or 4 after thawing. In the case of fertilization trials, similar percentages (around 80%) were found for control semen and for the majority of cryopreserved samples. However, statistically lower results (among 44%–60%) were found for Ex1DMSO (cryotubes) samples and for Ex1EG samples in both containers. In summary, the feasibility to cryopreserve pejerrey sperm was demonstrated for the first time using simple and practical protocols.Fil: Lichtenstein, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Elisio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Miranda, Leandro Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaElsevier Science2010-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243596Lichtenstein, Gabriel; Elisio, Mariano; Miranda, Leandro Andres; Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis; Elsevier Science; Aquaculture; 306; 1-4; 8-2010; 357-3610044-8486CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S004484861000325Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2010.05.016info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:05Zoai:ri.conicet.gov.ar:11336/243596instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:05.34CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis
title Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis
spellingShingle Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis
Lichtenstein, Gabriel
Cryopreservation
Sperm
Pejerrey
Reproduction
title_short Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis
title_full Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis
title_fullStr Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis
title_full_unstemmed Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis
title_sort Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis
dc.creator.none.fl_str_mv Lichtenstein, Gabriel
Elisio, Mariano
Miranda, Leandro Andres
author Lichtenstein, Gabriel
author_facet Lichtenstein, Gabriel
Elisio, Mariano
Miranda, Leandro Andres
author_role author
author2 Elisio, Mariano
Miranda, Leandro Andres
author2_role author
author
dc.subject.none.fl_str_mv Cryopreservation
Sperm
Pejerrey
Reproduction
topic Cryopreservation
Sperm
Pejerrey
Reproduction
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The pejerrey, Odontesthes bonariensis, is being considered for aquaculture due to its high demand and market price. Reproduction and larviculture studies have demonstrated the feasibility of massive fingerling production, and techniques that prolong life and increase gamete viability can assist in the culture development of this species. In this regard, the main objective of this study was to develop freezing protocols for pejerrey sperm. For this purpose, two extenders: Ex1, a modified Mounib solution (127 mM NaHCO3, 159 mM sucrose, 0.025 g/ml reduced glutathione; pH: 8; osmolality 400 mOsm/kg) and Ex2, a saline based solution (250 mM NaHCO3, 100 mM trehalose; pH: 8; osmolality 450 mOsm/kg) were developed. Dimethyl sulfoxide (DMSO) and ethylene glycol, (EG) were added at 10% as cryoprotectants and two types of containers were used: cryovials (1 ml of volume) and French straws (0.250 ml). Cryopreservation was made without equilibration time using dry ice or liquid nitrogen. The results obtained by freezing pejerrey semen with dry ice showed that all the combinations tested were suitable because high motility indexes (among 4 or 5) and good fertility percentages (between 46% and 56%) were obtained after thawing. However, the fertilization percentage obtained with control semen was significantly higher (80%). No significant statistical differences were observed on the fertilization percentages between experimental combinations or the containers used. In the case of liquid nitrogen as freezing method, it was possible to obtain for all the combinations motility indexes among 3 or 4 after thawing. In the case of fertilization trials, similar percentages (around 80%) were found for control semen and for the majority of cryopreserved samples. However, statistically lower results (among 44%–60%) were found for Ex1DMSO (cryotubes) samples and for Ex1EG samples in both containers. In summary, the feasibility to cryopreserve pejerrey sperm was demonstrated for the first time using simple and practical protocols.
Fil: Lichtenstein, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Elisio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Miranda, Leandro Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
description The pejerrey, Odontesthes bonariensis, is being considered for aquaculture due to its high demand and market price. Reproduction and larviculture studies have demonstrated the feasibility of massive fingerling production, and techniques that prolong life and increase gamete viability can assist in the culture development of this species. In this regard, the main objective of this study was to develop freezing protocols for pejerrey sperm. For this purpose, two extenders: Ex1, a modified Mounib solution (127 mM NaHCO3, 159 mM sucrose, 0.025 g/ml reduced glutathione; pH: 8; osmolality 400 mOsm/kg) and Ex2, a saline based solution (250 mM NaHCO3, 100 mM trehalose; pH: 8; osmolality 450 mOsm/kg) were developed. Dimethyl sulfoxide (DMSO) and ethylene glycol, (EG) were added at 10% as cryoprotectants and two types of containers were used: cryovials (1 ml of volume) and French straws (0.250 ml). Cryopreservation was made without equilibration time using dry ice or liquid nitrogen. The results obtained by freezing pejerrey semen with dry ice showed that all the combinations tested were suitable because high motility indexes (among 4 or 5) and good fertility percentages (between 46% and 56%) were obtained after thawing. However, the fertilization percentage obtained with control semen was significantly higher (80%). No significant statistical differences were observed on the fertilization percentages between experimental combinations or the containers used. In the case of liquid nitrogen as freezing method, it was possible to obtain for all the combinations motility indexes among 3 or 4 after thawing. In the case of fertilization trials, similar percentages (around 80%) were found for control semen and for the majority of cryopreserved samples. However, statistically lower results (among 44%–60%) were found for Ex1DMSO (cryotubes) samples and for Ex1EG samples in both containers. In summary, the feasibility to cryopreserve pejerrey sperm was demonstrated for the first time using simple and practical protocols.
publishDate 2010
dc.date.none.fl_str_mv 2010-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243596
Lichtenstein, Gabriel; Elisio, Mariano; Miranda, Leandro Andres; Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis; Elsevier Science; Aquaculture; 306; 1-4; 8-2010; 357-361
0044-8486
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243596
identifier_str_mv Lichtenstein, Gabriel; Elisio, Mariano; Miranda, Leandro Andres; Development of sperm cryopreservation techniques in pejerrey Odontesthes bonariensis; Elsevier Science; Aquaculture; 306; 1-4; 8-2010; 357-361
0044-8486
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S004484861000325X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aquaculture.2010.05.016
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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