Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuli

Autores
Achikanu, Cosmas; Correia, Joao; Guidobaldi, Héctor Alejandro; Giojalas, Laura Cecilia; Barratt, Christopher; Da Silva, Sarah Martins; Publicover, Stephen
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Human sperm show a variety of different behaviours (types of motility) that have different functional roles. Previous reports suggest that sperm may reversibly switch between these behaviours. We have recorded and analysed the behaviour of individual human sperm (180 cells in total), each cell monitored continuously for 3-3.5 min either under control conditions or in the presence of Ca2+-mobilising stimuli. Switching between different behaviours was assessed visually (1 s bins using four behaviour categories), and was verified by fractal dimension analysis of sperm head tracks. In the absence of stimuli, ~90% of cells showed at least one behavioural transition (mean rate under control conditions = 6.4 ± 0.8 transitions.min-1). Type 1 behaviour (progressive, activated-like motility) was most common, but the majority of cells (>70%) displayed at least three behaviour types. Treatment of sperm with Ca2+-mobilising agonists had negligible effects on the rate of switching but increased the time spent in type 2 and type 3 (hyperactivation-like) behaviours (P < 2∗10-8; chi-square). Treatment with 4-aminopyridine under alkaline conditions (pHo = 8.5), a highly-potent Ca2+-mobilising stimulus, was the most effective in increasing the proportion of type 3 behaviour, biasing switching away from type 1 (P < 0.005) and dramatically extending the duration of type 3 events (P < 10-16). Other stimuli, including 300 nM progesterone and 1% human follicular fluid, had qualitatively similar effects but were less potent. We conclude that human sperm observed in vitro constitutively display a range of behaviours and regulation of motility by [Ca2+]i, at the level of the single cell, is achieved not by causing cells to adopt a 'new' behaviour but by changing the relative contributions of those behaviours.
Fil: Achikanu, Cosmas. University of Birmingham; Reino Unido
Fil: Correia, Joao. University of Birmingham; Reino Unido
Fil: Guidobaldi, Héctor Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Giojalas, Laura Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Barratt, Christopher. University of Dundee; Reino Unido
Fil: Da Silva, Sarah Martins. University of Dundee; Reino Unido
Fil: Publicover, Stephen. University of Birmingham; Reino Unido
Materia
BEHAVIOUR
CALCIUM
MOTILITY
PH
SPERMATOZOA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/100724
Acceder
id CONICETDig_461cfd4ea10083ed353c8ee6d5e13d02
oai_identifier_str oai:ri.conicet.gov.ar:11336/100724
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuliAchikanu, CosmasCorreia, JoaoGuidobaldi, Héctor AlejandroGiojalas, Laura CeciliaBarratt, ChristopherDa Silva, Sarah MartinsPublicover, StephenBEHAVIOURCALCIUMMOTILITYPHSPERMATOZOAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Human sperm show a variety of different behaviours (types of motility) that have different functional roles. Previous reports suggest that sperm may reversibly switch between these behaviours. We have recorded and analysed the behaviour of individual human sperm (180 cells in total), each cell monitored continuously for 3-3.5 min either under control conditions or in the presence of Ca2+-mobilising stimuli. Switching between different behaviours was assessed visually (1 s bins using four behaviour categories), and was verified by fractal dimension analysis of sperm head tracks. In the absence of stimuli, ~90% of cells showed at least one behavioural transition (mean rate under control conditions = 6.4 ± 0.8 transitions.min-1). Type 1 behaviour (progressive, activated-like motility) was most common, but the majority of cells (>70%) displayed at least three behaviour types. Treatment of sperm with Ca2+-mobilising agonists had negligible effects on the rate of switching but increased the time spent in type 2 and type 3 (hyperactivation-like) behaviours (P < 2∗10-8; chi-square). Treatment with 4-aminopyridine under alkaline conditions (pHo = 8.5), a highly-potent Ca2+-mobilising stimulus, was the most effective in increasing the proportion of type 3 behaviour, biasing switching away from type 1 (P < 0.005) and dramatically extending the duration of type 3 events (P < 10-16). Other stimuli, including 300 nM progesterone and 1% human follicular fluid, had qualitatively similar effects but were less potent. We conclude that human sperm observed in vitro constitutively display a range of behaviours and regulation of motility by [Ca2+]i, at the level of the single cell, is achieved not by causing cells to adopt a 'new' behaviour but by changing the relative contributions of those behaviours.Fil: Achikanu, Cosmas. University of Birmingham; Reino UnidoFil: Correia, Joao. University of Birmingham; Reino UnidoFil: Guidobaldi, Héctor Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; ArgentinaFil: Giojalas, Laura Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Barratt, Christopher. University of Dundee; Reino UnidoFil: Da Silva, Sarah Martins. University of Dundee; Reino UnidoFil: Publicover, Stephen. University of Birmingham; Reino UnidoOxford University Press2019-07-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100724Achikanu, Cosmas; Correia, Joao; Guidobaldi, Héctor Alejandro; Giojalas, Laura Cecilia; Barratt, Christopher; et al.; Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuli; Oxford University Press; Molecular Human Reproduction; 25; 8; 13-7-2019; 423-4321360-99471460-2407CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/molehr/gaz034info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/molehr/article/25/8/423/5514444info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:22:26Zoai:ri.conicet.gov.ar:11336/100724instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:22:26.946CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuli
title Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuli
spellingShingle Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuli
Achikanu, Cosmas
BEHAVIOUR
CALCIUM
MOTILITY
PH
SPERMATOZOA
title_short Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuli
title_full Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuli
title_fullStr Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuli
title_full_unstemmed Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuli
title_sort Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuli
dc.creator.none.fl_str_mv Achikanu, Cosmas
Correia, Joao
Guidobaldi, Héctor Alejandro
Giojalas, Laura Cecilia
Barratt, Christopher
Da Silva, Sarah Martins
Publicover, Stephen
author Achikanu, Cosmas
author_facet Achikanu, Cosmas
Correia, Joao
Guidobaldi, Héctor Alejandro
Giojalas, Laura Cecilia
Barratt, Christopher
Da Silva, Sarah Martins
Publicover, Stephen
author_role author
author2 Correia, Joao
Guidobaldi, Héctor Alejandro
Giojalas, Laura Cecilia
Barratt, Christopher
Da Silva, Sarah Martins
Publicover, Stephen
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BEHAVIOUR
CALCIUM
MOTILITY
PH
SPERMATOZOA
topic BEHAVIOUR
CALCIUM
MOTILITY
PH
SPERMATOZOA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Human sperm show a variety of different behaviours (types of motility) that have different functional roles. Previous reports suggest that sperm may reversibly switch between these behaviours. We have recorded and analysed the behaviour of individual human sperm (180 cells in total), each cell monitored continuously for 3-3.5 min either under control conditions or in the presence of Ca2+-mobilising stimuli. Switching between different behaviours was assessed visually (1 s bins using four behaviour categories), and was verified by fractal dimension analysis of sperm head tracks. In the absence of stimuli, ~90% of cells showed at least one behavioural transition (mean rate under control conditions = 6.4 ± 0.8 transitions.min-1). Type 1 behaviour (progressive, activated-like motility) was most common, but the majority of cells (>70%) displayed at least three behaviour types. Treatment of sperm with Ca2+-mobilising agonists had negligible effects on the rate of switching but increased the time spent in type 2 and type 3 (hyperactivation-like) behaviours (P < 2∗10-8; chi-square). Treatment with 4-aminopyridine under alkaline conditions (pHo = 8.5), a highly-potent Ca2+-mobilising stimulus, was the most effective in increasing the proportion of type 3 behaviour, biasing switching away from type 1 (P < 0.005) and dramatically extending the duration of type 3 events (P < 10-16). Other stimuli, including 300 nM progesterone and 1% human follicular fluid, had qualitatively similar effects but were less potent. We conclude that human sperm observed in vitro constitutively display a range of behaviours and regulation of motility by [Ca2+]i, at the level of the single cell, is achieved not by causing cells to adopt a 'new' behaviour but by changing the relative contributions of those behaviours.
Fil: Achikanu, Cosmas. University of Birmingham; Reino Unido
Fil: Correia, Joao. University of Birmingham; Reino Unido
Fil: Guidobaldi, Héctor Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina
Fil: Giojalas, Laura Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Barratt, Christopher. University of Dundee; Reino Unido
Fil: Da Silva, Sarah Martins. University of Dundee; Reino Unido
Fil: Publicover, Stephen. University of Birmingham; Reino Unido
description Human sperm show a variety of different behaviours (types of motility) that have different functional roles. Previous reports suggest that sperm may reversibly switch between these behaviours. We have recorded and analysed the behaviour of individual human sperm (180 cells in total), each cell monitored continuously for 3-3.5 min either under control conditions or in the presence of Ca2+-mobilising stimuli. Switching between different behaviours was assessed visually (1 s bins using four behaviour categories), and was verified by fractal dimension analysis of sperm head tracks. In the absence of stimuli, ~90% of cells showed at least one behavioural transition (mean rate under control conditions = 6.4 ± 0.8 transitions.min-1). Type 1 behaviour (progressive, activated-like motility) was most common, but the majority of cells (>70%) displayed at least three behaviour types. Treatment of sperm with Ca2+-mobilising agonists had negligible effects on the rate of switching but increased the time spent in type 2 and type 3 (hyperactivation-like) behaviours (P < 2∗10-8; chi-square). Treatment with 4-aminopyridine under alkaline conditions (pHo = 8.5), a highly-potent Ca2+-mobilising stimulus, was the most effective in increasing the proportion of type 3 behaviour, biasing switching away from type 1 (P < 0.005) and dramatically extending the duration of type 3 events (P < 10-16). Other stimuli, including 300 nM progesterone and 1% human follicular fluid, had qualitatively similar effects but were less potent. We conclude that human sperm observed in vitro constitutively display a range of behaviours and regulation of motility by [Ca2+]i, at the level of the single cell, is achieved not by causing cells to adopt a 'new' behaviour but by changing the relative contributions of those behaviours.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/100724
Achikanu, Cosmas; Correia, Joao; Guidobaldi, Héctor Alejandro; Giojalas, Laura Cecilia; Barratt, Christopher; et al.; Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuli; Oxford University Press; Molecular Human Reproduction; 25; 8; 13-7-2019; 423-432
1360-9947
1460-2407
CONICET Digital
CONICET
url http://hdl.handle.net/11336/100724
identifier_str_mv Achikanu, Cosmas; Correia, Joao; Guidobaldi, Héctor Alejandro; Giojalas, Laura Cecilia; Barratt, Christopher; et al.; Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuli; Oxford University Press; Molecular Human Reproduction; 25; 8; 13-7-2019; 423-432
1360-9947
1460-2407
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/molehr/gaz034
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/molehr/article/25/8/423/5514444
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614215650770944
score 13.070432

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