A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy

Autores
Dong, Pin; Stellmacher, Johannes; Bouchet, Lydia María; Nieke, Marius; Kumar, Amit; Osorio-Blanco, Ernesto R.; Nagel, Gregor; Lohan, Silke B.; Teutloff, Christian; Patzelt, Alexa; Schäfer Korting, Monika; Calderón, Marcelo; Meinke, Martina C.; Alexiev, Ulrike
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Simultaneous visualization and concentration quantification of molecules in biological tissue is an important though challenging goal. The advantages of fluorescence lifetime imaging microscopy (FLIM) for visualization, and electron paramagnetic resonance (EPR) spectroscopy for quantification are complementary. Their combination in a multiplexed approach promises a successful but ambitious strategy because of spin label-mediated fluorescence quenching. Here, we solved this problem and present the molecular design of a dual label (DL) compound comprising a highly fluorescent dye together with an EPR spin probe, which also renders the fluorescence lifetime to be concentration sensitive. The DL can easily be coupled to the biomolecule of choice, enabling in vivo and in vitro applications. This novel approach paves the way for elegant studies ranging from fundamental biological investigations to preclinical drug research, as shown in proof-of-principle penetration experiments in human skin ex vivo.
Fil: Dong, Pin. Freie Universität Berlin; Alemania. Humboldt-Universität zu Berlin; Alemania
Fil: Stellmacher, Johannes. Freie Universität Berlin; Alemania
Fil: Bouchet, Lydia María. Freie Universität Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Nieke, Marius. Freie Universität Berlin; Alemania. Humboldt-Universität zu Berlin; Alemania
Fil: Kumar, Amit. Freie Universität Berlin; Alemania
Fil: Osorio-Blanco, Ernesto R.. Freie Universität Berlin; Alemania
Fil: Nagel, Gregor. Freie Universität Berlin; Alemania
Fil: Lohan, Silke B.. Humboldt-Universität zu Berlin; Alemania. Freie Universität Berlin; Alemania
Fil: Teutloff, Christian. Freie Universität Berlin; Alemania
Fil: Patzelt, Alexa. Humboldt-Universität zu Berlin; Alemania. Freie Universität Berlin; Alemania
Fil: Schäfer Korting, Monika. Freie Universität Berlin; Alemania
Fil: Calderón, Marcelo. Freie Universität Berlin; Alemania. Universidad del País Vasco; España
Fil: Meinke, Martina C.. Humboldt-Universität zu Berlin; Alemania. Freie Universität Berlin; Alemania
Fil: Alexiev, Ulrike. Freie Universität Berlin; Alemania
Materia
3-CARBOXY-PROXYL (PCA)
FLUORESCENCE
MULTIPLEXED FLIM–EPR SPECTROSCOPY
RHODAMINE B
SPIN LABELS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/172668
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/172668
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR SpectroscopyDong, PinStellmacher, JohannesBouchet, Lydia MaríaNieke, MariusKumar, AmitOsorio-Blanco, Ernesto R.Nagel, GregorLohan, Silke B.Teutloff, ChristianPatzelt, AlexaSchäfer Korting, MonikaCalderón, MarceloMeinke, Martina C.Alexiev, Ulrike3-CARBOXY-PROXYL (PCA)FLUORESCENCEMULTIPLEXED FLIM–EPR SPECTROSCOPYRHODAMINE BSPIN LABELShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Simultaneous visualization and concentration quantification of molecules in biological tissue is an important though challenging goal. The advantages of fluorescence lifetime imaging microscopy (FLIM) for visualization, and electron paramagnetic resonance (EPR) spectroscopy for quantification are complementary. Their combination in a multiplexed approach promises a successful but ambitious strategy because of spin label-mediated fluorescence quenching. Here, we solved this problem and present the molecular design of a dual label (DL) compound comprising a highly fluorescent dye together with an EPR spin probe, which also renders the fluorescence lifetime to be concentration sensitive. The DL can easily be coupled to the biomolecule of choice, enabling in vivo and in vitro applications. This novel approach paves the way for elegant studies ranging from fundamental biological investigations to preclinical drug research, as shown in proof-of-principle penetration experiments in human skin ex vivo.Fil: Dong, Pin. Freie Universität Berlin; Alemania. Humboldt-Universität zu Berlin; AlemaniaFil: Stellmacher, Johannes. Freie Universität Berlin; AlemaniaFil: Bouchet, Lydia María. Freie Universität Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Nieke, Marius. Freie Universität Berlin; Alemania. Humboldt-Universität zu Berlin; AlemaniaFil: Kumar, Amit. Freie Universität Berlin; AlemaniaFil: Osorio-Blanco, Ernesto R.. Freie Universität Berlin; AlemaniaFil: Nagel, Gregor. Freie Universität Berlin; AlemaniaFil: Lohan, Silke B.. Humboldt-Universität zu Berlin; Alemania. Freie Universität Berlin; AlemaniaFil: Teutloff, Christian. Freie Universität Berlin; AlemaniaFil: Patzelt, Alexa. Humboldt-Universität zu Berlin; Alemania. Freie Universität Berlin; AlemaniaFil: Schäfer Korting, Monika. Freie Universität Berlin; AlemaniaFil: Calderón, Marcelo. Freie Universität Berlin; Alemania. Universidad del País Vasco; EspañaFil: Meinke, Martina C.. Humboldt-Universität zu Berlin; Alemania. Freie Universität Berlin; AlemaniaFil: Alexiev, Ulrike. Freie Universität Berlin; AlemaniaWiley VCH Verlag2021-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/172668Dong, Pin; Stellmacher, Johannes; Bouchet, Lydia María; Nieke, Marius; Kumar, Amit; et al.; A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy; Wiley VCH Verlag; Angewandte Chemie; 60; 27; 6-2021; 14938-149441433-78511521-3773CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/anie.202012852info:eu-repo/semantics/altIdentifier/doi/10.1002/anie.202012852info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:21:14Zoai:ri.conicet.gov.ar:11336/172668instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:21:14.981CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy
title A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy
spellingShingle A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy
Dong, Pin
3-CARBOXY-PROXYL (PCA)
FLUORESCENCE
MULTIPLEXED FLIM–EPR SPECTROSCOPY
RHODAMINE B
SPIN LABELS
title_short A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy
title_full A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy
title_fullStr A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy
title_full_unstemmed A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy
title_sort A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy
dc.creator.none.fl_str_mv Dong, Pin
Stellmacher, Johannes
Bouchet, Lydia María
Nieke, Marius
Kumar, Amit
Osorio-Blanco, Ernesto R.
Nagel, Gregor
Lohan, Silke B.
Teutloff, Christian
Patzelt, Alexa
Schäfer Korting, Monika
Calderón, Marcelo
Meinke, Martina C.
Alexiev, Ulrike
author Dong, Pin
author_facet Dong, Pin
Stellmacher, Johannes
Bouchet, Lydia María
Nieke, Marius
Kumar, Amit
Osorio-Blanco, Ernesto R.
Nagel, Gregor
Lohan, Silke B.
Teutloff, Christian
Patzelt, Alexa
Schäfer Korting, Monika
Calderón, Marcelo
Meinke, Martina C.
Alexiev, Ulrike
author_role author
author2 Stellmacher, Johannes
Bouchet, Lydia María
Nieke, Marius
Kumar, Amit
Osorio-Blanco, Ernesto R.
Nagel, Gregor
Lohan, Silke B.
Teutloff, Christian
Patzelt, Alexa
Schäfer Korting, Monika
Calderón, Marcelo
Meinke, Martina C.
Alexiev, Ulrike
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 3-CARBOXY-PROXYL (PCA)
FLUORESCENCE
MULTIPLEXED FLIM–EPR SPECTROSCOPY
RHODAMINE B
SPIN LABELS
topic 3-CARBOXY-PROXYL (PCA)
FLUORESCENCE
MULTIPLEXED FLIM–EPR SPECTROSCOPY
RHODAMINE B
SPIN LABELS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Simultaneous visualization and concentration quantification of molecules in biological tissue is an important though challenging goal. The advantages of fluorescence lifetime imaging microscopy (FLIM) for visualization, and electron paramagnetic resonance (EPR) spectroscopy for quantification are complementary. Their combination in a multiplexed approach promises a successful but ambitious strategy because of spin label-mediated fluorescence quenching. Here, we solved this problem and present the molecular design of a dual label (DL) compound comprising a highly fluorescent dye together with an EPR spin probe, which also renders the fluorescence lifetime to be concentration sensitive. The DL can easily be coupled to the biomolecule of choice, enabling in vivo and in vitro applications. This novel approach paves the way for elegant studies ranging from fundamental biological investigations to preclinical drug research, as shown in proof-of-principle penetration experiments in human skin ex vivo.
Fil: Dong, Pin. Freie Universität Berlin; Alemania. Humboldt-Universität zu Berlin; Alemania
Fil: Stellmacher, Johannes. Freie Universität Berlin; Alemania
Fil: Bouchet, Lydia María. Freie Universität Berlin; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Nieke, Marius. Freie Universität Berlin; Alemania. Humboldt-Universität zu Berlin; Alemania
Fil: Kumar, Amit. Freie Universität Berlin; Alemania
Fil: Osorio-Blanco, Ernesto R.. Freie Universität Berlin; Alemania
Fil: Nagel, Gregor. Freie Universität Berlin; Alemania
Fil: Lohan, Silke B.. Humboldt-Universität zu Berlin; Alemania. Freie Universität Berlin; Alemania
Fil: Teutloff, Christian. Freie Universität Berlin; Alemania
Fil: Patzelt, Alexa. Humboldt-Universität zu Berlin; Alemania. Freie Universität Berlin; Alemania
Fil: Schäfer Korting, Monika. Freie Universität Berlin; Alemania
Fil: Calderón, Marcelo. Freie Universität Berlin; Alemania. Universidad del País Vasco; España
Fil: Meinke, Martina C.. Humboldt-Universität zu Berlin; Alemania. Freie Universität Berlin; Alemania
Fil: Alexiev, Ulrike. Freie Universität Berlin; Alemania
description Simultaneous visualization and concentration quantification of molecules in biological tissue is an important though challenging goal. The advantages of fluorescence lifetime imaging microscopy (FLIM) for visualization, and electron paramagnetic resonance (EPR) spectroscopy for quantification are complementary. Their combination in a multiplexed approach promises a successful but ambitious strategy because of spin label-mediated fluorescence quenching. Here, we solved this problem and present the molecular design of a dual label (DL) compound comprising a highly fluorescent dye together with an EPR spin probe, which also renders the fluorescence lifetime to be concentration sensitive. The DL can easily be coupled to the biomolecule of choice, enabling in vivo and in vitro applications. This novel approach paves the way for elegant studies ranging from fundamental biological investigations to preclinical drug research, as shown in proof-of-principle penetration experiments in human skin ex vivo.
publishDate 2021
dc.date.none.fl_str_mv 2021-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/172668
Dong, Pin; Stellmacher, Johannes; Bouchet, Lydia María; Nieke, Marius; Kumar, Amit; et al.; A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy; Wiley VCH Verlag; Angewandte Chemie; 60; 27; 6-2021; 14938-14944
1433-7851
1521-3773
CONICET Digital
CONICET
url http://hdl.handle.net/11336/172668
identifier_str_mv Dong, Pin; Stellmacher, Johannes; Bouchet, Lydia María; Nieke, Marius; Kumar, Amit; et al.; A Dual Fluorescence–Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM–EPR Spectroscopy; Wiley VCH Verlag; Angewandte Chemie; 60; 27; 6-2021; 14938-14944
1433-7851
1521-3773
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/anie.202012852
info:eu-repo/semantics/altIdentifier/doi/10.1002/anie.202012852
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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