Pitfalls in using fluorescence tagging of nanomaterials: Tecto-dendrimers in skin tissue as investigated by cluster-FLIM

Autores
Volz, Pierre; Schilrreff, Priscila; Brodwolf, Robert; Wolff, Christopher; Stellmacher, Johannes; Balke, Jens; Morilla, María José; Zoschke, Christian; Schäfer-Korting, Monika; Alexiev, Ulrike
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Targeted topical application promises high drug concentrations in the skin and low systemic adverse effects. To locate drugs and drug‐delivery systems like nanocarriers, fluorescent dyes are commonly used as drug surrogates or nanocarrier labels in micrographs of tissue sections. Here, we investigate how labeling degree, concentration of fluorophore, and nanocarrier may affect the interpretation of these micrographs. False‐negative penetration results due to inter‐ and intramolecular quenching effects are likely. Using tecto‐dendrimers as an example, we present a detailed analysis of pitfalls in the (semi‐)quantitative evaluation of skin nanocarrier penetration. Fluorescence lifetime imaging microscopy (FLIM) allows distinguishing the target fluorescence of dye‐tagged nanocarriers from skin autofluorescence, providing a highly sensitive tool for clear‐cut localization of the nanocarriers. Cluster‐FLIM images reveal that FITC‐labeled tecto‐dendrimers penetrate the stratum corneum of human skin ex vivo and reconstructed human skin but do not cross the tight junction barrier.
Fil: Volz, Pierre. Freie Universitat Berlin; Alemania
Fil: Schilrreff, Priscila. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brodwolf, Robert. Freie Universitat Berlin; Alemania
Fil: Wolff, Christopher. Freie Universitat Berlin; Alemania
Fil: Stellmacher, Johannes. Freie Universitat Berlin; Alemania
Fil: Balke, Jens. Freie Universitat Berlin; Alemania
Fil: Morilla, María José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zoschke, Christian. Freie Universitat Berlin; Alemania
Fil: Schäfer-Korting, Monika. Freie Universitat Berlin; Alemania
Fil: Alexiev, Ulrike. Freie Universitat Berlin; Alemania
Materia
Biomacromolecules
Drug Delivery System
Flim
Reconstructed Human Skin
Tight Junctions
Time-Resolved Fluorescence Spectroscopy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42383
Acceder
id CONICETDig_f46dace26548d51c1e6e50378372217d
oai_identifier_str oai:ri.conicet.gov.ar:11336/42383
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pitfalls in using fluorescence tagging of nanomaterials: Tecto-dendrimers in skin tissue as investigated by cluster-FLIMVolz, PierreSchilrreff, PriscilaBrodwolf, RobertWolff, ChristopherStellmacher, JohannesBalke, JensMorilla, María JoséZoschke, ChristianSchäfer-Korting, MonikaAlexiev, UlrikeBiomacromoleculesDrug Delivery SystemFlimReconstructed Human SkinTight JunctionsTime-Resolved Fluorescence Spectroscopyhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Targeted topical application promises high drug concentrations in the skin and low systemic adverse effects. To locate drugs and drug‐delivery systems like nanocarriers, fluorescent dyes are commonly used as drug surrogates or nanocarrier labels in micrographs of tissue sections. Here, we investigate how labeling degree, concentration of fluorophore, and nanocarrier may affect the interpretation of these micrographs. False‐negative penetration results due to inter‐ and intramolecular quenching effects are likely. Using tecto‐dendrimers as an example, we present a detailed analysis of pitfalls in the (semi‐)quantitative evaluation of skin nanocarrier penetration. Fluorescence lifetime imaging microscopy (FLIM) allows distinguishing the target fluorescence of dye‐tagged nanocarriers from skin autofluorescence, providing a highly sensitive tool for clear‐cut localization of the nanocarriers. Cluster‐FLIM images reveal that FITC‐labeled tecto‐dendrimers penetrate the stratum corneum of human skin ex vivo and reconstructed human skin but do not cross the tight junction barrier.Fil: Volz, Pierre. Freie Universitat Berlin; AlemaniaFil: Schilrreff, Priscila. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brodwolf, Robert. Freie Universitat Berlin; AlemaniaFil: Wolff, Christopher. Freie Universitat Berlin; AlemaniaFil: Stellmacher, Johannes. Freie Universitat Berlin; AlemaniaFil: Balke, Jens. Freie Universitat Berlin; AlemaniaFil: Morilla, María José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zoschke, Christian. Freie Universitat Berlin; AlemaniaFil: Schäfer-Korting, Monika. Freie Universitat Berlin; AlemaniaFil: Alexiev, Ulrike. Freie Universitat Berlin; AlemaniaBlackwell Publishing2017-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42383Volz, Pierre; Schilrreff, Priscila; Brodwolf, Robert; Wolff, Christopher; Stellmacher, Johannes; et al.; Pitfalls in using fluorescence tagging of nanomaterials: Tecto-dendrimers in skin tissue as investigated by cluster-FLIM; Blackwell Publishing; Annals of the New York Academy of Sciences; 1405; 1; 10-2017; 202-2140077-8923CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/nyas.13473info:eu-repo/semantics/altIdentifier/url/https://nyaspubs.onlinelibrary.wiley.com/doi/abs/10.1111/nyas.13473info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:25:14Zoai:ri.conicet.gov.ar:11336/42383instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:25:14.557CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pitfalls in using fluorescence tagging of nanomaterials: Tecto-dendrimers in skin tissue as investigated by cluster-FLIM
title Pitfalls in using fluorescence tagging of nanomaterials: Tecto-dendrimers in skin tissue as investigated by cluster-FLIM
spellingShingle Pitfalls in using fluorescence tagging of nanomaterials: Tecto-dendrimers in skin tissue as investigated by cluster-FLIM
Volz, Pierre
Biomacromolecules
Drug Delivery System
Flim
Reconstructed Human Skin
Tight Junctions
Time-Resolved Fluorescence Spectroscopy
title_short Pitfalls in using fluorescence tagging of nanomaterials: Tecto-dendrimers in skin tissue as investigated by cluster-FLIM
title_full Pitfalls in using fluorescence tagging of nanomaterials: Tecto-dendrimers in skin tissue as investigated by cluster-FLIM
title_fullStr Pitfalls in using fluorescence tagging of nanomaterials: Tecto-dendrimers in skin tissue as investigated by cluster-FLIM
title_full_unstemmed Pitfalls in using fluorescence tagging of nanomaterials: Tecto-dendrimers in skin tissue as investigated by cluster-FLIM
title_sort Pitfalls in using fluorescence tagging of nanomaterials: Tecto-dendrimers in skin tissue as investigated by cluster-FLIM
dc.creator.none.fl_str_mv Volz, Pierre
Schilrreff, Priscila
Brodwolf, Robert
Wolff, Christopher
Stellmacher, Johannes
Balke, Jens
Morilla, María José
Zoschke, Christian
Schäfer-Korting, Monika
Alexiev, Ulrike
author Volz, Pierre
author_facet Volz, Pierre
Schilrreff, Priscila
Brodwolf, Robert
Wolff, Christopher
Stellmacher, Johannes
Balke, Jens
Morilla, María José
Zoschke, Christian
Schäfer-Korting, Monika
Alexiev, Ulrike
author_role author
author2 Schilrreff, Priscila
Brodwolf, Robert
Wolff, Christopher
Stellmacher, Johannes
Balke, Jens
Morilla, María José
Zoschke, Christian
Schäfer-Korting, Monika
Alexiev, Ulrike
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biomacromolecules
Drug Delivery System
Flim
Reconstructed Human Skin
Tight Junctions
Time-Resolved Fluorescence Spectroscopy
topic Biomacromolecules
Drug Delivery System
Flim
Reconstructed Human Skin
Tight Junctions
Time-Resolved Fluorescence Spectroscopy
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Targeted topical application promises high drug concentrations in the skin and low systemic adverse effects. To locate drugs and drug‐delivery systems like nanocarriers, fluorescent dyes are commonly used as drug surrogates or nanocarrier labels in micrographs of tissue sections. Here, we investigate how labeling degree, concentration of fluorophore, and nanocarrier may affect the interpretation of these micrographs. False‐negative penetration results due to inter‐ and intramolecular quenching effects are likely. Using tecto‐dendrimers as an example, we present a detailed analysis of pitfalls in the (semi‐)quantitative evaluation of skin nanocarrier penetration. Fluorescence lifetime imaging microscopy (FLIM) allows distinguishing the target fluorescence of dye‐tagged nanocarriers from skin autofluorescence, providing a highly sensitive tool for clear‐cut localization of the nanocarriers. Cluster‐FLIM images reveal that FITC‐labeled tecto‐dendrimers penetrate the stratum corneum of human skin ex vivo and reconstructed human skin but do not cross the tight junction barrier.
Fil: Volz, Pierre. Freie Universitat Berlin; Alemania
Fil: Schilrreff, Priscila. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brodwolf, Robert. Freie Universitat Berlin; Alemania
Fil: Wolff, Christopher. Freie Universitat Berlin; Alemania
Fil: Stellmacher, Johannes. Freie Universitat Berlin; Alemania
Fil: Balke, Jens. Freie Universitat Berlin; Alemania
Fil: Morilla, María José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zoschke, Christian. Freie Universitat Berlin; Alemania
Fil: Schäfer-Korting, Monika. Freie Universitat Berlin; Alemania
Fil: Alexiev, Ulrike. Freie Universitat Berlin; Alemania
description Targeted topical application promises high drug concentrations in the skin and low systemic adverse effects. To locate drugs and drug‐delivery systems like nanocarriers, fluorescent dyes are commonly used as drug surrogates or nanocarrier labels in micrographs of tissue sections. Here, we investigate how labeling degree, concentration of fluorophore, and nanocarrier may affect the interpretation of these micrographs. False‐negative penetration results due to inter‐ and intramolecular quenching effects are likely. Using tecto‐dendrimers as an example, we present a detailed analysis of pitfalls in the (semi‐)quantitative evaluation of skin nanocarrier penetration. Fluorescence lifetime imaging microscopy (FLIM) allows distinguishing the target fluorescence of dye‐tagged nanocarriers from skin autofluorescence, providing a highly sensitive tool for clear‐cut localization of the nanocarriers. Cluster‐FLIM images reveal that FITC‐labeled tecto‐dendrimers penetrate the stratum corneum of human skin ex vivo and reconstructed human skin but do not cross the tight junction barrier.
publishDate 2017
dc.date.none.fl_str_mv 2017-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42383
Volz, Pierre; Schilrreff, Priscila; Brodwolf, Robert; Wolff, Christopher; Stellmacher, Johannes; et al.; Pitfalls in using fluorescence tagging of nanomaterials: Tecto-dendrimers in skin tissue as investigated by cluster-FLIM; Blackwell Publishing; Annals of the New York Academy of Sciences; 1405; 1; 10-2017; 202-214
0077-8923
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42383
identifier_str_mv Volz, Pierre; Schilrreff, Priscila; Brodwolf, Robert; Wolff, Christopher; Stellmacher, Johannes; et al.; Pitfalls in using fluorescence tagging of nanomaterials: Tecto-dendrimers in skin tissue as investigated by cluster-FLIM; Blackwell Publishing; Annals of the New York Academy of Sciences; 1405; 1; 10-2017; 202-214
0077-8923
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/nyas.13473
info:eu-repo/semantics/altIdentifier/url/https://nyaspubs.onlinelibrary.wiley.com/doi/abs/10.1111/nyas.13473
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981399803461632
score 12.493442

Publicaciones similares