On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments

Autores
Priotto, Santiago; Lara, Ruben Jose
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present work different approaches were tested for the optimization of the hydrolysis conditions for the joint determination of amino acids and amino sugars in chitin, an important structural polymer in fungi, invertebrates, algae and the exoskeleton of zooplankton. Ten treatments were evaluated, involving different combinations of hydrolysis temperature and duration. The obtained results indicate that slightly lower temperatures (105ºC) than for the classical combined amino acid hydrolysis and longer times (22 hours) than for chitin minimized losses and maximized recoveries of monomeric amino acids and amino sugars. This represents a large advantage in comparison to the usual methods of separate hydrolysis for high-performance liquid chromatography (HPLC) determination, allowing a simultaneous analysis of both compound classes in a single run.
Fil: Priotto, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina
Fil: Lara, Ruben Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina. Leibniz Centre for Tropical Marine Ecology; Alemania
Materia
Marine Sediments
Amino Acids
Amino Sugars
Determination
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2999
Acceder
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network_name_str CONICET Digital (CONICET)
spelling On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine SedimentsPriotto, SantiagoLara, Ruben JoseMarine SedimentsAmino AcidsAmino SugarsDeterminationhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1In the present work different approaches were tested for the optimization of the hydrolysis conditions for the joint determination of amino acids and amino sugars in chitin, an important structural polymer in fungi, invertebrates, algae and the exoskeleton of zooplankton. Ten treatments were evaluated, involving different combinations of hydrolysis temperature and duration. The obtained results indicate that slightly lower temperatures (105ºC) than for the classical combined amino acid hydrolysis and longer times (22 hours) than for chitin minimized losses and maximized recoveries of monomeric amino acids and amino sugars. This represents a large advantage in comparison to the usual methods of separate hydrolysis for high-performance liquid chromatography (HPLC) determination, allowing a simultaneous analysis of both compound classes in a single run.Fil: Priotto, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); ArgentinaFil: Lara, Ruben Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina. Leibniz Centre for Tropical Marine Ecology; AlemaniaSciTecnhol2013-07-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2999Priotto, Santiago; Lara, Ruben Jose; On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments; SciTecnhol; Journal of Marine Biology & Oceanography; 2; 3; 18-7-2013; 1-52324-8661enginfo:eu-repo/semantics/altIdentifier/url/http://www.scitechnol.com/on-optimization-hydrolysis-conditions-for-simultaneous-determination-amino-acids-and-amino-sugars-marine-sediments-8KfH.php?article_id=1331info:eu-repo/semantics/altIdentifier/doi/10.4172/2324-8661.1000112info:eu-repo/semantics/altIdentifier/issn/2324-8661info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:36:21Zoai:ri.conicet.gov.ar:11336/2999instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:36:22.108CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments
title On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments
spellingShingle On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments
Priotto, Santiago
Marine Sediments
Amino Acids
Amino Sugars
Determination
title_short On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments
title_full On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments
title_fullStr On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments
title_full_unstemmed On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments
title_sort On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments
dc.creator.none.fl_str_mv Priotto, Santiago
Lara, Ruben Jose
author Priotto, Santiago
author_facet Priotto, Santiago
Lara, Ruben Jose
author_role author
author2 Lara, Ruben Jose
author2_role author
dc.subject.none.fl_str_mv Marine Sediments
Amino Acids
Amino Sugars
Determination
topic Marine Sediments
Amino Acids
Amino Sugars
Determination
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the present work different approaches were tested for the optimization of the hydrolysis conditions for the joint determination of amino acids and amino sugars in chitin, an important structural polymer in fungi, invertebrates, algae and the exoskeleton of zooplankton. Ten treatments were evaluated, involving different combinations of hydrolysis temperature and duration. The obtained results indicate that slightly lower temperatures (105ºC) than for the classical combined amino acid hydrolysis and longer times (22 hours) than for chitin minimized losses and maximized recoveries of monomeric amino acids and amino sugars. This represents a large advantage in comparison to the usual methods of separate hydrolysis for high-performance liquid chromatography (HPLC) determination, allowing a simultaneous analysis of both compound classes in a single run.
Fil: Priotto, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina
Fil: Lara, Ruben Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina. Leibniz Centre for Tropical Marine Ecology; Alemania
description In the present work different approaches were tested for the optimization of the hydrolysis conditions for the joint determination of amino acids and amino sugars in chitin, an important structural polymer in fungi, invertebrates, algae and the exoskeleton of zooplankton. Ten treatments were evaluated, involving different combinations of hydrolysis temperature and duration. The obtained results indicate that slightly lower temperatures (105ºC) than for the classical combined amino acid hydrolysis and longer times (22 hours) than for chitin minimized losses and maximized recoveries of monomeric amino acids and amino sugars. This represents a large advantage in comparison to the usual methods of separate hydrolysis for high-performance liquid chromatography (HPLC) determination, allowing a simultaneous analysis of both compound classes in a single run.
publishDate 2013
dc.date.none.fl_str_mv 2013-07-18
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2999
Priotto, Santiago; Lara, Ruben Jose; On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments; SciTecnhol; Journal of Marine Biology & Oceanography; 2; 3; 18-7-2013; 1-5
2324-8661
url http://hdl.handle.net/11336/2999
identifier_str_mv Priotto, Santiago; Lara, Ruben Jose; On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments; SciTecnhol; Journal of Marine Biology & Oceanography; 2; 3; 18-7-2013; 1-5
2324-8661
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scitechnol.com/on-optimization-hydrolysis-conditions-for-simultaneous-determination-amino-acids-and-amino-sugars-marine-sediments-8KfH.php?article_id=1331
info:eu-repo/semantics/altIdentifier/doi/10.4172/2324-8661.1000112
info:eu-repo/semantics/altIdentifier/issn/2324-8661
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv SciTecnhol
publisher.none.fl_str_mv SciTecnhol
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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