On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments
- Autores
- Priotto, Santiago; Lara, Ruben Jose
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In the present work different approaches were tested for the optimization of the hydrolysis conditions for the joint determination of amino acids and amino sugars in chitin, an important structural polymer in fungi, invertebrates, algae and the exoskeleton of zooplankton. Ten treatments were evaluated, involving different combinations of hydrolysis temperature and duration. The obtained results indicate that slightly lower temperatures (105ºC) than for the classical combined amino acid hydrolysis and longer times (22 hours) than for chitin minimized losses and maximized recoveries of monomeric amino acids and amino sugars. This represents a large advantage in comparison to the usual methods of separate hydrolysis for high-performance liquid chromatography (HPLC) determination, allowing a simultaneous analysis of both compound classes in a single run.
Fil: Priotto, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina
Fil: Lara, Ruben Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina. Leibniz Centre for Tropical Marine Ecology; Alemania - Materia
-
Marine Sediments
Amino Acids
Amino Sugars
Determination - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/2999
Ver los metadatos del registro completo
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On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine SedimentsPriotto, SantiagoLara, Ruben JoseMarine SedimentsAmino AcidsAmino SugarsDeterminationhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1In the present work different approaches were tested for the optimization of the hydrolysis conditions for the joint determination of amino acids and amino sugars in chitin, an important structural polymer in fungi, invertebrates, algae and the exoskeleton of zooplankton. Ten treatments were evaluated, involving different combinations of hydrolysis temperature and duration. The obtained results indicate that slightly lower temperatures (105ºC) than for the classical combined amino acid hydrolysis and longer times (22 hours) than for chitin minimized losses and maximized recoveries of monomeric amino acids and amino sugars. This represents a large advantage in comparison to the usual methods of separate hydrolysis for high-performance liquid chromatography (HPLC) determination, allowing a simultaneous analysis of both compound classes in a single run.Fil: Priotto, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); ArgentinaFil: Lara, Ruben Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina. Leibniz Centre for Tropical Marine Ecology; AlemaniaSciTecnhol2013-07-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2999Priotto, Santiago; Lara, Ruben Jose; On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments; SciTecnhol; Journal of Marine Biology & Oceanography; 2; 3; 18-7-2013; 1-52324-8661enginfo:eu-repo/semantics/altIdentifier/url/http://www.scitechnol.com/on-optimization-hydrolysis-conditions-for-simultaneous-determination-amino-acids-and-amino-sugars-marine-sediments-8KfH.php?article_id=1331info:eu-repo/semantics/altIdentifier/doi/10.4172/2324-8661.1000112info:eu-repo/semantics/altIdentifier/issn/2324-8661info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:10Zoai:ri.conicet.gov.ar:11336/2999instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:10.983CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments |
title |
On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments |
spellingShingle |
On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments Priotto, Santiago Marine Sediments Amino Acids Amino Sugars Determination |
title_short |
On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments |
title_full |
On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments |
title_fullStr |
On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments |
title_full_unstemmed |
On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments |
title_sort |
On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments |
dc.creator.none.fl_str_mv |
Priotto, Santiago Lara, Ruben Jose |
author |
Priotto, Santiago |
author_facet |
Priotto, Santiago Lara, Ruben Jose |
author_role |
author |
author2 |
Lara, Ruben Jose |
author2_role |
author |
dc.subject.none.fl_str_mv |
Marine Sediments Amino Acids Amino Sugars Determination |
topic |
Marine Sediments Amino Acids Amino Sugars Determination |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.5 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In the present work different approaches were tested for the optimization of the hydrolysis conditions for the joint determination of amino acids and amino sugars in chitin, an important structural polymer in fungi, invertebrates, algae and the exoskeleton of zooplankton. Ten treatments were evaluated, involving different combinations of hydrolysis temperature and duration. The obtained results indicate that slightly lower temperatures (105ºC) than for the classical combined amino acid hydrolysis and longer times (22 hours) than for chitin minimized losses and maximized recoveries of monomeric amino acids and amino sugars. This represents a large advantage in comparison to the usual methods of separate hydrolysis for high-performance liquid chromatography (HPLC) determination, allowing a simultaneous analysis of both compound classes in a single run. Fil: Priotto, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina Fil: Lara, Ruben Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto Argentino de Oceanografía (i); Argentina. Leibniz Centre for Tropical Marine Ecology; Alemania |
description |
In the present work different approaches were tested for the optimization of the hydrolysis conditions for the joint determination of amino acids and amino sugars in chitin, an important structural polymer in fungi, invertebrates, algae and the exoskeleton of zooplankton. Ten treatments were evaluated, involving different combinations of hydrolysis temperature and duration. The obtained results indicate that slightly lower temperatures (105ºC) than for the classical combined amino acid hydrolysis and longer times (22 hours) than for chitin minimized losses and maximized recoveries of monomeric amino acids and amino sugars. This represents a large advantage in comparison to the usual methods of separate hydrolysis for high-performance liquid chromatography (HPLC) determination, allowing a simultaneous analysis of both compound classes in a single run. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-18 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/2999 Priotto, Santiago; Lara, Ruben Jose; On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments; SciTecnhol; Journal of Marine Biology & Oceanography; 2; 3; 18-7-2013; 1-5 2324-8661 |
url |
http://hdl.handle.net/11336/2999 |
identifier_str_mv |
Priotto, Santiago; Lara, Ruben Jose; On the Optimization of Hydrolysis Conditions for Simultaneous Determination of Amino Acids and Amino Sugars in Marine Sediments; SciTecnhol; Journal of Marine Biology & Oceanography; 2; 3; 18-7-2013; 1-5 2324-8661 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.scitechnol.com/on-optimization-hydrolysis-conditions-for-simultaneous-determination-amino-acids-and-amino-sugars-marine-sediments-8KfH.php?article_id=1331 info:eu-repo/semantics/altIdentifier/doi/10.4172/2324-8661.1000112 info:eu-repo/semantics/altIdentifier/issn/2324-8661 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
SciTecnhol |
publisher.none.fl_str_mv |
SciTecnhol |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |