Role of amino acids and endogenous lipids in sperm capacitation of porcine spermatozoa

Autores
Satorre, María Mercedes; Rodriguez, Pablo Cristian; Breininger, Elizabeth; Cetica, Pablo Daniel
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The role of oxidative substrates and metabolic pathways in providing energy for porcine sperm capacitation is not yet fully understood. Our aim was to study the role of amino acids and endogenous lipids as oxidative substrates in porcine sperm capacitation, comparing them to classical oxidative substrates such as glucose and pyruvate. Sperm samples were incubated in capacitation media with or without classical oxidative substrates. Amino acids were added in the presence or absence of salicylate, an oxidative deamination inhibitor, to assess their potential as oxidative substrates. To evaluate endogenous lipid consumption, L-carnitine (a fatty acid β-oxidation inducer) and etomoxir (a fatty acid β-oxidation inhibitor) were utilized. Sperm motility, viability, capacitation, and ammonia production were evaluated for each treatment. In capacitation media without oxidative substrates, spermatozoa preserved motility and viability but failed to undergo capacitation. The addition of amino acids to the medium without oxidative substrates increased ammonia production but did not support sperm capacitation and diminished sperm motility. These effects were not observed when glucose and pyruvate were present in culture medium, although ammonia production was still increased. The addition of L-carnitine to the medium without oxidative substrates significantly improved sperm capacitation, whereas etomoxir had no effect. Boar spermatozoa have deamination activity, but amino acids by themselves cannot sustain sperm capacitation. Contrarily, catabolism of endogenous lipids can partially support sperm capacitation when they are the only oxidative substrates available. This study provides new insights into the role of metabolic pathways during porcine sperm capacitation and has significant implications for the development of assisted reproductive technologies in this species.
Fil: Satorre, María Mercedes. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
Fil: Rodriguez, Pablo Cristian. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
Fil: Breininger, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Materia
Amino acids
Endogenous lipids
Porcine
Sperm capacitation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/280839
Acceder
id CONICETDig_ad23eb31061022bbde157820bd8aa417
oai_identifier_str oai:ri.conicet.gov.ar:11336/280839
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of amino acids and endogenous lipids in sperm capacitation of porcine spermatozoaSatorre, María MercedesRodriguez, Pablo CristianBreininger, ElizabethCetica, Pablo DanielAmino acidsEndogenous lipidsPorcineSperm capacitationhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The role of oxidative substrates and metabolic pathways in providing energy for porcine sperm capacitation is not yet fully understood. Our aim was to study the role of amino acids and endogenous lipids as oxidative substrates in porcine sperm capacitation, comparing them to classical oxidative substrates such as glucose and pyruvate. Sperm samples were incubated in capacitation media with or without classical oxidative substrates. Amino acids were added in the presence or absence of salicylate, an oxidative deamination inhibitor, to assess their potential as oxidative substrates. To evaluate endogenous lipid consumption, L-carnitine (a fatty acid β-oxidation inducer) and etomoxir (a fatty acid β-oxidation inhibitor) were utilized. Sperm motility, viability, capacitation, and ammonia production were evaluated for each treatment. In capacitation media without oxidative substrates, spermatozoa preserved motility and viability but failed to undergo capacitation. The addition of amino acids to the medium without oxidative substrates increased ammonia production but did not support sperm capacitation and diminished sperm motility. These effects were not observed when glucose and pyruvate were present in culture medium, although ammonia production was still increased. The addition of L-carnitine to the medium without oxidative substrates significantly improved sperm capacitation, whereas etomoxir had no effect. Boar spermatozoa have deamination activity, but amino acids by themselves cannot sustain sperm capacitation. Contrarily, catabolism of endogenous lipids can partially support sperm capacitation when they are the only oxidative substrates available. This study provides new insights into the role of metabolic pathways during porcine sperm capacitation and has significant implications for the development of assisted reproductive technologies in this species.Fil: Satorre, María Mercedes. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; ArgentinaFil: Rodriguez, Pablo Cristian. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; ArgentinaFil: Breininger, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaAssiut University. Faculty of Veterinary Medicine. Department of Animal Medicine2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/280839Satorre, María Mercedes; Rodriguez, Pablo Cristian; Breininger, Elizabeth; Cetica, Pablo Daniel; Role of amino acids and endogenous lipids in sperm capacitation of porcine spermatozoa; Assiut University. Faculty of Veterinary Medicine. Department of Animal Medicine; Journal of Advanced Veterinary Research; 15; 1; 1-2025; 81-862090-6269CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://advetresearch.com/index.php/AVR/article/view/2095info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T12:59:46Zoai:ri.conicet.gov.ar:11336/280839instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 12:59:46.375CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of amino acids and endogenous lipids in sperm capacitation of porcine spermatozoa
title Role of amino acids and endogenous lipids in sperm capacitation of porcine spermatozoa
spellingShingle Role of amino acids and endogenous lipids in sperm capacitation of porcine spermatozoa
Satorre, María Mercedes
Amino acids
Endogenous lipids
Porcine
Sperm capacitation
title_short Role of amino acids and endogenous lipids in sperm capacitation of porcine spermatozoa
title_full Role of amino acids and endogenous lipids in sperm capacitation of porcine spermatozoa
title_fullStr Role of amino acids and endogenous lipids in sperm capacitation of porcine spermatozoa
title_full_unstemmed Role of amino acids and endogenous lipids in sperm capacitation of porcine spermatozoa
title_sort Role of amino acids and endogenous lipids in sperm capacitation of porcine spermatozoa
dc.creator.none.fl_str_mv Satorre, María Mercedes
Rodriguez, Pablo Cristian
Breininger, Elizabeth
Cetica, Pablo Daniel
author Satorre, María Mercedes
author_facet Satorre, María Mercedes
Rodriguez, Pablo Cristian
Breininger, Elizabeth
Cetica, Pablo Daniel
author_role author
author2 Rodriguez, Pablo Cristian
Breininger, Elizabeth
Cetica, Pablo Daniel
author2_role author
author
author
dc.subject.none.fl_str_mv Amino acids
Endogenous lipids
Porcine
Sperm capacitation
topic Amino acids
Endogenous lipids
Porcine
Sperm capacitation
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The role of oxidative substrates and metabolic pathways in providing energy for porcine sperm capacitation is not yet fully understood. Our aim was to study the role of amino acids and endogenous lipids as oxidative substrates in porcine sperm capacitation, comparing them to classical oxidative substrates such as glucose and pyruvate. Sperm samples were incubated in capacitation media with or without classical oxidative substrates. Amino acids were added in the presence or absence of salicylate, an oxidative deamination inhibitor, to assess their potential as oxidative substrates. To evaluate endogenous lipid consumption, L-carnitine (a fatty acid β-oxidation inducer) and etomoxir (a fatty acid β-oxidation inhibitor) were utilized. Sperm motility, viability, capacitation, and ammonia production were evaluated for each treatment. In capacitation media without oxidative substrates, spermatozoa preserved motility and viability but failed to undergo capacitation. The addition of amino acids to the medium without oxidative substrates increased ammonia production but did not support sperm capacitation and diminished sperm motility. These effects were not observed when glucose and pyruvate were present in culture medium, although ammonia production was still increased. The addition of L-carnitine to the medium without oxidative substrates significantly improved sperm capacitation, whereas etomoxir had no effect. Boar spermatozoa have deamination activity, but amino acids by themselves cannot sustain sperm capacitation. Contrarily, catabolism of endogenous lipids can partially support sperm capacitation when they are the only oxidative substrates available. This study provides new insights into the role of metabolic pathways during porcine sperm capacitation and has significant implications for the development of assisted reproductive technologies in this species.
Fil: Satorre, María Mercedes. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
Fil: Rodriguez, Pablo Cristian. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal; Argentina
Fil: Breininger, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
description The role of oxidative substrates and metabolic pathways in providing energy for porcine sperm capacitation is not yet fully understood. Our aim was to study the role of amino acids and endogenous lipids as oxidative substrates in porcine sperm capacitation, comparing them to classical oxidative substrates such as glucose and pyruvate. Sperm samples were incubated in capacitation media with or without classical oxidative substrates. Amino acids were added in the presence or absence of salicylate, an oxidative deamination inhibitor, to assess their potential as oxidative substrates. To evaluate endogenous lipid consumption, L-carnitine (a fatty acid β-oxidation inducer) and etomoxir (a fatty acid β-oxidation inhibitor) were utilized. Sperm motility, viability, capacitation, and ammonia production were evaluated for each treatment. In capacitation media without oxidative substrates, spermatozoa preserved motility and viability but failed to undergo capacitation. The addition of amino acids to the medium without oxidative substrates increased ammonia production but did not support sperm capacitation and diminished sperm motility. These effects were not observed when glucose and pyruvate were present in culture medium, although ammonia production was still increased. The addition of L-carnitine to the medium without oxidative substrates significantly improved sperm capacitation, whereas etomoxir had no effect. Boar spermatozoa have deamination activity, but amino acids by themselves cannot sustain sperm capacitation. Contrarily, catabolism of endogenous lipids can partially support sperm capacitation when they are the only oxidative substrates available. This study provides new insights into the role of metabolic pathways during porcine sperm capacitation and has significant implications for the development of assisted reproductive technologies in this species.
publishDate 2025
dc.date.none.fl_str_mv 2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/280839
Satorre, María Mercedes; Rodriguez, Pablo Cristian; Breininger, Elizabeth; Cetica, Pablo Daniel; Role of amino acids and endogenous lipids in sperm capacitation of porcine spermatozoa; Assiut University. Faculty of Veterinary Medicine. Department of Animal Medicine; Journal of Advanced Veterinary Research; 15; 1; 1-2025; 81-86
2090-6269
CONICET Digital
CONICET
url http://hdl.handle.net/11336/280839
identifier_str_mv Satorre, María Mercedes; Rodriguez, Pablo Cristian; Breininger, Elizabeth; Cetica, Pablo Daniel; Role of amino acids and endogenous lipids in sperm capacitation of porcine spermatozoa; Assiut University. Faculty of Veterinary Medicine. Department of Animal Medicine; Journal of Advanced Veterinary Research; 15; 1; 1-2025; 81-86
2090-6269
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://advetresearch.com/index.php/AVR/article/view/2095
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Assiut University. Faculty of Veterinary Medicine. Department of Animal Medicine
publisher.none.fl_str_mv Assiut University. Faculty of Veterinary Medicine. Department of Animal Medicine
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.106097

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