Effect of amino acids and peptides on growth of pediococcus pentosaceus from wine

Autores
Aredes Fernández, Pedro Adrián; Saguir de Zucal, Fabiana Maria; Manca, Maria Cristina
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactic acid bacteria are characterized by numerous nutritional requirements. The influence of amino acids and peptides on the growth of Pediococcus pentosaceus c1 isolated from argentinean wines was determined. Cells were growth in synthetic media and in the same media added with the following dipeptides: leucine-leucine; leucine-proline; methionine-proline and glycineglycine. In the complete medium, P. pentosaceus c1 reached a final biomass of 1x10(8) 6.5pt. When leucine, methionine and glycine were independently removed from complete medium, cell biomass was lower than 90% indicating that these amino acids are essentials to bacterial growth. In absence of proline the parameters of growth were similar to those obtained on control medium, indicating that proline was not essential for bacterial growth. The growth was partially restored in the medium without methionine (80 %), after addition of the dipeptide methionine proline, and a growth of 90 % was reached by the addition of leucine-proline to the medium without leucine. When leucine-leucine was added to the medium without leucine, 100 % of cell growth was observed and when glycine-glycine was incorporated to the medium without glycine, growth parameters were increased. Dipeptides could supply the essential amino acid requirements of P. pentosaceus c1. The lower specific growth rate (20 %) and cell biomass (70 %) obtained when the four dipeptides were added to the medium without leucine, methionine, glycine and proline, suggest that a limited uptake of amino acids by P. pentosaceus c1 could be caused by a substantial concentrations of dipeptides.
Fil: Aredes Fernández, Pedro Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Saguir de Zucal, Fabiana Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Manca, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
PEDIOCOCCUS PENTOSACEUS
WINE
AMINO ACIDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/57834
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/57834
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of amino acids and peptides on growth of pediococcus pentosaceus from wineAredes Fernández, Pedro AdriánSaguir de Zucal, Fabiana MariaManca, Maria CristinaPEDIOCOCCUS PENTOSACEUSWINEAMINO ACIDShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lactic acid bacteria are characterized by numerous nutritional requirements. The influence of amino acids and peptides on the growth of Pediococcus pentosaceus c1 isolated from argentinean wines was determined. Cells were growth in synthetic media and in the same media added with the following dipeptides: leucine-leucine; leucine-proline; methionine-proline and glycineglycine. In the complete medium, P. pentosaceus c1 reached a final biomass of 1x10(8) 6.5pt. When leucine, methionine and glycine were independently removed from complete medium, cell biomass was lower than 90% indicating that these amino acids are essentials to bacterial growth. In absence of proline the parameters of growth were similar to those obtained on control medium, indicating that proline was not essential for bacterial growth. The growth was partially restored in the medium without methionine (80 %), after addition of the dipeptide methionine proline, and a growth of 90 % was reached by the addition of leucine-proline to the medium without leucine. When leucine-leucine was added to the medium without leucine, 100 % of cell growth was observed and when glycine-glycine was incorporated to the medium without glycine, growth parameters were increased. Dipeptides could supply the essential amino acid requirements of P. pentosaceus c1. The lower specific growth rate (20 %) and cell biomass (70 %) obtained when the four dipeptides were added to the medium without leucine, methionine, glycine and proline, suggest that a limited uptake of amino acids by P. pentosaceus c1 could be caused by a substantial concentrations of dipeptides.Fil: Aredes Fernández, Pedro Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Saguir de Zucal, Fabiana Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Manca, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaPlapiqui2003-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/57834Aredes Fernández, Pedro Adrián; Saguir de Zucal, Fabiana Maria; Manca, Maria Cristina; Effect of amino acids and peptides on growth of pediococcus pentosaceus from wine; Plapiqui; Latin American Applied Research; 33; 9-2003; 225-2290327-07931851-8796CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.laar.uns.edu.ar/indexes/artic_v3303/p225.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:29Zoai:ri.conicet.gov.ar:11336/57834instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:29.3CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of amino acids and peptides on growth of pediococcus pentosaceus from wine
title Effect of amino acids and peptides on growth of pediococcus pentosaceus from wine
spellingShingle Effect of amino acids and peptides on growth of pediococcus pentosaceus from wine
Aredes Fernández, Pedro Adrián
PEDIOCOCCUS PENTOSACEUS
WINE
AMINO ACIDS
title_short Effect of amino acids and peptides on growth of pediococcus pentosaceus from wine
title_full Effect of amino acids and peptides on growth of pediococcus pentosaceus from wine
title_fullStr Effect of amino acids and peptides on growth of pediococcus pentosaceus from wine
title_full_unstemmed Effect of amino acids and peptides on growth of pediococcus pentosaceus from wine
title_sort Effect of amino acids and peptides on growth of pediococcus pentosaceus from wine
dc.creator.none.fl_str_mv Aredes Fernández, Pedro Adrián
Saguir de Zucal, Fabiana Maria
Manca, Maria Cristina
author Aredes Fernández, Pedro Adrián
author_facet Aredes Fernández, Pedro Adrián
Saguir de Zucal, Fabiana Maria
Manca, Maria Cristina
author_role author
author2 Saguir de Zucal, Fabiana Maria
Manca, Maria Cristina
author2_role author
author
dc.subject.none.fl_str_mv PEDIOCOCCUS PENTOSACEUS
WINE
AMINO ACIDS
topic PEDIOCOCCUS PENTOSACEUS
WINE
AMINO ACIDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lactic acid bacteria are characterized by numerous nutritional requirements. The influence of amino acids and peptides on the growth of Pediococcus pentosaceus c1 isolated from argentinean wines was determined. Cells were growth in synthetic media and in the same media added with the following dipeptides: leucine-leucine; leucine-proline; methionine-proline and glycineglycine. In the complete medium, P. pentosaceus c1 reached a final biomass of 1x10(8) 6.5pt. When leucine, methionine and glycine were independently removed from complete medium, cell biomass was lower than 90% indicating that these amino acids are essentials to bacterial growth. In absence of proline the parameters of growth were similar to those obtained on control medium, indicating that proline was not essential for bacterial growth. The growth was partially restored in the medium without methionine (80 %), after addition of the dipeptide methionine proline, and a growth of 90 % was reached by the addition of leucine-proline to the medium without leucine. When leucine-leucine was added to the medium without leucine, 100 % of cell growth was observed and when glycine-glycine was incorporated to the medium without glycine, growth parameters were increased. Dipeptides could supply the essential amino acid requirements of P. pentosaceus c1. The lower specific growth rate (20 %) and cell biomass (70 %) obtained when the four dipeptides were added to the medium without leucine, methionine, glycine and proline, suggest that a limited uptake of amino acids by P. pentosaceus c1 could be caused by a substantial concentrations of dipeptides.
Fil: Aredes Fernández, Pedro Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Saguir de Zucal, Fabiana Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Manca, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description Lactic acid bacteria are characterized by numerous nutritional requirements. The influence of amino acids and peptides on the growth of Pediococcus pentosaceus c1 isolated from argentinean wines was determined. Cells were growth in synthetic media and in the same media added with the following dipeptides: leucine-leucine; leucine-proline; methionine-proline and glycineglycine. In the complete medium, P. pentosaceus c1 reached a final biomass of 1x10(8) 6.5pt. When leucine, methionine and glycine were independently removed from complete medium, cell biomass was lower than 90% indicating that these amino acids are essentials to bacterial growth. In absence of proline the parameters of growth were similar to those obtained on control medium, indicating that proline was not essential for bacterial growth. The growth was partially restored in the medium without methionine (80 %), after addition of the dipeptide methionine proline, and a growth of 90 % was reached by the addition of leucine-proline to the medium without leucine. When leucine-leucine was added to the medium without leucine, 100 % of cell growth was observed and when glycine-glycine was incorporated to the medium without glycine, growth parameters were increased. Dipeptides could supply the essential amino acid requirements of P. pentosaceus c1. The lower specific growth rate (20 %) and cell biomass (70 %) obtained when the four dipeptides were added to the medium without leucine, methionine, glycine and proline, suggest that a limited uptake of amino acids by P. pentosaceus c1 could be caused by a substantial concentrations of dipeptides.
publishDate 2003
dc.date.none.fl_str_mv 2003-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/57834
Aredes Fernández, Pedro Adrián; Saguir de Zucal, Fabiana Maria; Manca, Maria Cristina; Effect of amino acids and peptides on growth of pediococcus pentosaceus from wine; Plapiqui; Latin American Applied Research; 33; 9-2003; 225-229
0327-0793
1851-8796
CONICET Digital
CONICET
url http://hdl.handle.net/11336/57834
identifier_str_mv Aredes Fernández, Pedro Adrián; Saguir de Zucal, Fabiana Maria; Manca, Maria Cristina; Effect of amino acids and peptides on growth of pediococcus pentosaceus from wine; Plapiqui; Latin American Applied Research; 33; 9-2003; 225-229
0327-0793
1851-8796
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.laar.uns.edu.ar/indexes/artic_v3303/p225.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Plapiqui
publisher.none.fl_str_mv Plapiqui
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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