Perinatal exposure to diethylstilbestrol alters the functional differentiation of the adult rat uterus

Autores
Bosquiazzo, Veronica Lis; Vigezzi, Lucía; Muñoz de Toro, Mónica; Luque, Enrique Hugo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The exposure to endocrine disrupters and female reproductive tract disorders has not been totally clarified. The present study assessed the long-term effect of perinatal (gestation + lactation) exposure to diethylstilbestrol (DES) on the rat uterus and the effect of estrogen replacement therapy. DES (5 g/kg bw/day) was administered in the drinking water from gestational day 9 until weaning and we studied the uterus of young adult (PND90) and adult (PND360) females. To investigate whether perinatal exposure to DES modified the uterine response to a long-lasting estrogen treatment, 12-month-old rats exposed to DES were ovariectomized and treated with 17-estradiol for 3 months (PND460). In young adult rats (PND90), the DES treatment decreased both the proliferation of glandular epithelial cells and the percentage of glandular perimeter occupied by -smooth muscle actin-positive cells. The other tissue compartments remained unchanged. Cell apoptosis was not altered in DES-exposed females. In control adult rats (PND360), there were some morphologically abnormal uterine glands. In adult rats exposed to DES, the incidence of glands with cellular anomalies increased. In response to estrogens (PND460), the incidence of cystic glands increased in the DES group. We observed glands with daughter glands and conglomerates of glands only on PND460 and in response to estrogen replacement therapy, independently of DES exposure. The p63 isoforms were expressed without changes on PND460. Estrogen receptors and showed no changes, while the progesterone receptor decreased in the subepithelial stroma of DESexposed animals with estrogen treatment. The long-lasting effects of perinatal exposure to DES included the induction of abnormalities in uterine tissues of aged female rats and an altered response of the adult uterus to estradiol.
Fil: Bosquiazzo, Veronica Lis. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Vigezzi, Lucía. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Muñoz de Toro, Mónica. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Luque, Enrique Hugo. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
Materia
Diethylstilbestrol
Uterus
Steroid Receptors
Uterine Glands
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6402
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/6402
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Perinatal exposure to diethylstilbestrol alters the functional differentiation of the adult rat uterusBosquiazzo, Veronica LisVigezzi, LucíaMuñoz de Toro, MónicaLuque, Enrique HugoDiethylstilbestrolUterusSteroid ReceptorsUterine Glandshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The exposure to endocrine disrupters and female reproductive tract disorders has not been totally clarified. The present study assessed the long-term effect of perinatal (gestation + lactation) exposure to diethylstilbestrol (DES) on the rat uterus and the effect of estrogen replacement therapy. DES (5 g/kg bw/day) was administered in the drinking water from gestational day 9 until weaning and we studied the uterus of young adult (PND90) and adult (PND360) females. To investigate whether perinatal exposure to DES modified the uterine response to a long-lasting estrogen treatment, 12-month-old rats exposed to DES were ovariectomized and treated with 17-estradiol for 3 months (PND460). In young adult rats (PND90), the DES treatment decreased both the proliferation of glandular epithelial cells and the percentage of glandular perimeter occupied by -smooth muscle actin-positive cells. The other tissue compartments remained unchanged. Cell apoptosis was not altered in DES-exposed females. In control adult rats (PND360), there were some morphologically abnormal uterine glands. In adult rats exposed to DES, the incidence of glands with cellular anomalies increased. In response to estrogens (PND460), the incidence of cystic glands increased in the DES group. We observed glands with daughter glands and conglomerates of glands only on PND460 and in response to estrogen replacement therapy, independently of DES exposure. The p63 isoforms were expressed without changes on PND460. Estrogen receptors and showed no changes, while the progesterone receptor decreased in the subepithelial stroma of DESexposed animals with estrogen treatment. The long-lasting effects of perinatal exposure to DES included the induction of abnormalities in uterine tissues of aged female rats and an altered response of the adult uterus to estradiol.Fil: Bosquiazzo, Veronica Lis. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; ArgentinaFil: Vigezzi, Lucía. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; ArgentinaFil: Muñoz de Toro, Mónica. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; ArgentinaFil: Luque, Enrique Hugo. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; ArgentinaElsevier2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6402Bosquiazzo, Veronica Lis; Vigezzi, Lucía; Muñoz de Toro, Mónica; Luque, Enrique Hugo; Perinatal exposure to diethylstilbestrol alters the functional differentiation of the adult rat uterus; Elsevier; Journal of Steroid Biochemistry and Molecular Biology; 138; 2-2013; 1-90960-0760enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0960076013000423info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jsbmb.2013.02.011info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:11Zoai:ri.conicet.gov.ar:11336/6402instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:12.202CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Perinatal exposure to diethylstilbestrol alters the functional differentiation of the adult rat uterus
title Perinatal exposure to diethylstilbestrol alters the functional differentiation of the adult rat uterus
spellingShingle Perinatal exposure to diethylstilbestrol alters the functional differentiation of the adult rat uterus
Bosquiazzo, Veronica Lis
Diethylstilbestrol
Uterus
Steroid Receptors
Uterine Glands
title_short Perinatal exposure to diethylstilbestrol alters the functional differentiation of the adult rat uterus
title_full Perinatal exposure to diethylstilbestrol alters the functional differentiation of the adult rat uterus
title_fullStr Perinatal exposure to diethylstilbestrol alters the functional differentiation of the adult rat uterus
title_full_unstemmed Perinatal exposure to diethylstilbestrol alters the functional differentiation of the adult rat uterus
title_sort Perinatal exposure to diethylstilbestrol alters the functional differentiation of the adult rat uterus
dc.creator.none.fl_str_mv Bosquiazzo, Veronica Lis
Vigezzi, Lucía
Muñoz de Toro, Mónica
Luque, Enrique Hugo
author Bosquiazzo, Veronica Lis
author_facet Bosquiazzo, Veronica Lis
Vigezzi, Lucía
Muñoz de Toro, Mónica
Luque, Enrique Hugo
author_role author
author2 Vigezzi, Lucía
Muñoz de Toro, Mónica
Luque, Enrique Hugo
author2_role author
author
author
dc.subject.none.fl_str_mv Diethylstilbestrol
Uterus
Steroid Receptors
Uterine Glands
topic Diethylstilbestrol
Uterus
Steroid Receptors
Uterine Glands
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The exposure to endocrine disrupters and female reproductive tract disorders has not been totally clarified. The present study assessed the long-term effect of perinatal (gestation + lactation) exposure to diethylstilbestrol (DES) on the rat uterus and the effect of estrogen replacement therapy. DES (5 g/kg bw/day) was administered in the drinking water from gestational day 9 until weaning and we studied the uterus of young adult (PND90) and adult (PND360) females. To investigate whether perinatal exposure to DES modified the uterine response to a long-lasting estrogen treatment, 12-month-old rats exposed to DES were ovariectomized and treated with 17-estradiol for 3 months (PND460). In young adult rats (PND90), the DES treatment decreased both the proliferation of glandular epithelial cells and the percentage of glandular perimeter occupied by -smooth muscle actin-positive cells. The other tissue compartments remained unchanged. Cell apoptosis was not altered in DES-exposed females. In control adult rats (PND360), there were some morphologically abnormal uterine glands. In adult rats exposed to DES, the incidence of glands with cellular anomalies increased. In response to estrogens (PND460), the incidence of cystic glands increased in the DES group. We observed glands with daughter glands and conglomerates of glands only on PND460 and in response to estrogen replacement therapy, independently of DES exposure. The p63 isoforms were expressed without changes on PND460. Estrogen receptors and showed no changes, while the progesterone receptor decreased in the subepithelial stroma of DESexposed animals with estrogen treatment. The long-lasting effects of perinatal exposure to DES included the induction of abnormalities in uterine tissues of aged female rats and an altered response of the adult uterus to estradiol.
Fil: Bosquiazzo, Veronica Lis. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Vigezzi, Lucía. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Muñoz de Toro, Mónica. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina
Fil: Luque, Enrique Hugo. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Departamento de Fisiologia. Laboratorio de Endocrinologia y Tumores Hormonodependientes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; Argentina
description The exposure to endocrine disrupters and female reproductive tract disorders has not been totally clarified. The present study assessed the long-term effect of perinatal (gestation + lactation) exposure to diethylstilbestrol (DES) on the rat uterus and the effect of estrogen replacement therapy. DES (5 g/kg bw/day) was administered in the drinking water from gestational day 9 until weaning and we studied the uterus of young adult (PND90) and adult (PND360) females. To investigate whether perinatal exposure to DES modified the uterine response to a long-lasting estrogen treatment, 12-month-old rats exposed to DES were ovariectomized and treated with 17-estradiol for 3 months (PND460). In young adult rats (PND90), the DES treatment decreased both the proliferation of glandular epithelial cells and the percentage of glandular perimeter occupied by -smooth muscle actin-positive cells. The other tissue compartments remained unchanged. Cell apoptosis was not altered in DES-exposed females. In control adult rats (PND360), there were some morphologically abnormal uterine glands. In adult rats exposed to DES, the incidence of glands with cellular anomalies increased. In response to estrogens (PND460), the incidence of cystic glands increased in the DES group. We observed glands with daughter glands and conglomerates of glands only on PND460 and in response to estrogen replacement therapy, independently of DES exposure. The p63 isoforms were expressed without changes on PND460. Estrogen receptors and showed no changes, while the progesterone receptor decreased in the subepithelial stroma of DESexposed animals with estrogen treatment. The long-lasting effects of perinatal exposure to DES included the induction of abnormalities in uterine tissues of aged female rats and an altered response of the adult uterus to estradiol.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6402
Bosquiazzo, Veronica Lis; Vigezzi, Lucía; Muñoz de Toro, Mónica; Luque, Enrique Hugo; Perinatal exposure to diethylstilbestrol alters the functional differentiation of the adult rat uterus; Elsevier; Journal of Steroid Biochemistry and Molecular Biology; 138; 2-2013; 1-9
0960-0760
url http://hdl.handle.net/11336/6402
identifier_str_mv Bosquiazzo, Veronica Lis; Vigezzi, Lucía; Muñoz de Toro, Mónica; Luque, Enrique Hugo; Perinatal exposure to diethylstilbestrol alters the functional differentiation of the adult rat uterus; Elsevier; Journal of Steroid Biochemistry and Molecular Biology; 138; 2-2013; 1-9
0960-0760
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0960076013000423
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jsbmb.2013.02.011
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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