Prenatal exposure to bisphenol A promotes angiogenesis and alters steroid-mediated responses in the mammary glands of cycling rats

Autores
Durando, Milena de Lourdes; Kass, Laura; Perdomo, Virginia; Bosquiazzo, Veronica Lis; Luque, Enrique Hugo; Muñoz de Toro, Monica Milagros
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Prenatal exposure to BPA disturbs mammary gland histoarchitecture and increases thecarcinogenic susceptibility to chemical challenges administered long after BPA exposure.Our aim was to assess the effect of prenatal BPA exposure on mammary glandangiogenesis and steroid hormone pathways in virgin cycling rats. Pregnant Wistar ratswere exposed to either 25 or 250 μg/kg/day (25 and 250 BPA, respectively) or to vehicle.Female offspring were autopsied on postnatal day (PND) 50 or 110. Ovarian steroid serumlevels, the expression of steroid receptors and their co-regulators SRC-3 and SMRT in themammary gland, and angiogenesis were evaluated. At PND 50, all BPA-treated animalshad lower serum levels of progesterone, while estradiol levels remained unchanged. Thehigher dose of BPA increased mammary ERa and decreased SRC-3 expression at PND 50and PND 110. SMRT protein levels were similar among groups at PND 50, whereas atPND 110, animals exposed to 250 BPA showed a lower SMRT expression. Interestingly, inthe control and 25 BPA groups, SMRT increased from PND 50 to PND 110. At PND 50, anincreased vascular area associated with higher VEGF expression was observed in the 250BPA-treated rats. At PND 110, the vascular area was still increased, but VEGF expressionwas similar to that of control rats. The present results demonstrate that prenatal exposure toBPA alters the endocrine environment of the mammary gland and its angiogenic process.Increased angiogenesis and altered steroid hormone signals could explain the higherfrequency of pre-neoplastic lesions found later in life.
Fil: Durando, Milena de Lourdes. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Fil: Kass, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Perdomo, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Bosquiazzo, Veronica Lis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Muñoz de Toro, Monica Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Materia
ANGIOGENESIS
BISPHENOL A
ENDOCRINE DISRUPTORS
HYPERPLASTIC DUCTS
MAMMARY GLANDS
STEROID RECEPTORS
STEROID RECPETOR CO-ACTIVATORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103199
Acceder
id CONICETDig_efbcebfdb69204fae76404f2646d5a8d
oai_identifier_str oai:ri.conicet.gov.ar:11336/103199
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Prenatal exposure to bisphenol A promotes angiogenesis and alters steroid-mediated responses in the mammary glands of cycling ratsDurando, Milena de LourdesKass, LauraPerdomo, VirginiaBosquiazzo, Veronica LisLuque, Enrique HugoMuñoz de Toro, Monica MilagrosANGIOGENESISBISPHENOL AENDOCRINE DISRUPTORSHYPERPLASTIC DUCTSMAMMARY GLANDSSTEROID RECEPTORSSTEROID RECPETOR CO-ACTIVATORShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Prenatal exposure to BPA disturbs mammary gland histoarchitecture and increases thecarcinogenic susceptibility to chemical challenges administered long after BPA exposure.Our aim was to assess the effect of prenatal BPA exposure on mammary glandangiogenesis and steroid hormone pathways in virgin cycling rats. Pregnant Wistar ratswere exposed to either 25 or 250 μg/kg/day (25 and 250 BPA, respectively) or to vehicle.Female offspring were autopsied on postnatal day (PND) 50 or 110. Ovarian steroid serumlevels, the expression of steroid receptors and their co-regulators SRC-3 and SMRT in themammary gland, and angiogenesis were evaluated. At PND 50, all BPA-treated animalshad lower serum levels of progesterone, while estradiol levels remained unchanged. Thehigher dose of BPA increased mammary ERa and decreased SRC-3 expression at PND 50and PND 110. SMRT protein levels were similar among groups at PND 50, whereas atPND 110, animals exposed to 250 BPA showed a lower SMRT expression. Interestingly, inthe control and 25 BPA groups, SMRT increased from PND 50 to PND 110. At PND 50, anincreased vascular area associated with higher VEGF expression was observed in the 250BPA-treated rats. At PND 110, the vascular area was still increased, but VEGF expressionwas similar to that of control rats. The present results demonstrate that prenatal exposure toBPA alters the endocrine environment of the mammary gland and its angiogenic process.Increased angiogenesis and altered steroid hormone signals could explain the higherfrequency of pre-neoplastic lesions found later in life.Fil: Durando, Milena de Lourdes. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; ArgentinaFil: Kass, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Perdomo, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Bosquiazzo, Veronica Lis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Muñoz de Toro, Monica Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaPergamon-Elsevier Science Ltd2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103199Durando, Milena de Lourdes; Kass, Laura; Perdomo, Virginia; Bosquiazzo, Veronica Lis; Luque, Enrique Hugo; et al.; Prenatal exposure to bisphenol A promotes angiogenesis and alters steroid-mediated responses in the mammary glands of cycling rats; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 127; 1-2; 10-2011; 35-430960-0760CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0960076011000823info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jsbmb.2011.04.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:34Zoai:ri.conicet.gov.ar:11336/103199instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:35.316CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prenatal exposure to bisphenol A promotes angiogenesis and alters steroid-mediated responses in the mammary glands of cycling rats
title Prenatal exposure to bisphenol A promotes angiogenesis and alters steroid-mediated responses in the mammary glands of cycling rats
spellingShingle Prenatal exposure to bisphenol A promotes angiogenesis and alters steroid-mediated responses in the mammary glands of cycling rats
Durando, Milena de Lourdes
ANGIOGENESIS
BISPHENOL A
ENDOCRINE DISRUPTORS
HYPERPLASTIC DUCTS
MAMMARY GLANDS
STEROID RECEPTORS
STEROID RECPETOR CO-ACTIVATORS
title_short Prenatal exposure to bisphenol A promotes angiogenesis and alters steroid-mediated responses in the mammary glands of cycling rats
title_full Prenatal exposure to bisphenol A promotes angiogenesis and alters steroid-mediated responses in the mammary glands of cycling rats
title_fullStr Prenatal exposure to bisphenol A promotes angiogenesis and alters steroid-mediated responses in the mammary glands of cycling rats
title_full_unstemmed Prenatal exposure to bisphenol A promotes angiogenesis and alters steroid-mediated responses in the mammary glands of cycling rats
title_sort Prenatal exposure to bisphenol A promotes angiogenesis and alters steroid-mediated responses in the mammary glands of cycling rats
dc.creator.none.fl_str_mv Durando, Milena de Lourdes
Kass, Laura
Perdomo, Virginia
Bosquiazzo, Veronica Lis
Luque, Enrique Hugo
Muñoz de Toro, Monica Milagros
author Durando, Milena de Lourdes
author_facet Durando, Milena de Lourdes
Kass, Laura
Perdomo, Virginia
Bosquiazzo, Veronica Lis
Luque, Enrique Hugo
Muñoz de Toro, Monica Milagros
author_role author
author2 Kass, Laura
Perdomo, Virginia
Bosquiazzo, Veronica Lis
Luque, Enrique Hugo
Muñoz de Toro, Monica Milagros
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ANGIOGENESIS
BISPHENOL A
ENDOCRINE DISRUPTORS
HYPERPLASTIC DUCTS
MAMMARY GLANDS
STEROID RECEPTORS
STEROID RECPETOR CO-ACTIVATORS
topic ANGIOGENESIS
BISPHENOL A
ENDOCRINE DISRUPTORS
HYPERPLASTIC DUCTS
MAMMARY GLANDS
STEROID RECEPTORS
STEROID RECPETOR CO-ACTIVATORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Prenatal exposure to BPA disturbs mammary gland histoarchitecture and increases thecarcinogenic susceptibility to chemical challenges administered long after BPA exposure.Our aim was to assess the effect of prenatal BPA exposure on mammary glandangiogenesis and steroid hormone pathways in virgin cycling rats. Pregnant Wistar ratswere exposed to either 25 or 250 μg/kg/day (25 and 250 BPA, respectively) or to vehicle.Female offspring were autopsied on postnatal day (PND) 50 or 110. Ovarian steroid serumlevels, the expression of steroid receptors and their co-regulators SRC-3 and SMRT in themammary gland, and angiogenesis were evaluated. At PND 50, all BPA-treated animalshad lower serum levels of progesterone, while estradiol levels remained unchanged. Thehigher dose of BPA increased mammary ERa and decreased SRC-3 expression at PND 50and PND 110. SMRT protein levels were similar among groups at PND 50, whereas atPND 110, animals exposed to 250 BPA showed a lower SMRT expression. Interestingly, inthe control and 25 BPA groups, SMRT increased from PND 50 to PND 110. At PND 50, anincreased vascular area associated with higher VEGF expression was observed in the 250BPA-treated rats. At PND 110, the vascular area was still increased, but VEGF expressionwas similar to that of control rats. The present results demonstrate that prenatal exposure toBPA alters the endocrine environment of the mammary gland and its angiogenic process.Increased angiogenesis and altered steroid hormone signals could explain the higherfrequency of pre-neoplastic lesions found later in life.
Fil: Durando, Milena de Lourdes. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Fisiología. Laboratorio de Endocrinología y Tumores Hormonodependientes; Argentina
Fil: Kass, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Perdomo, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Bosquiazzo, Veronica Lis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Muñoz de Toro, Monica Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
description Prenatal exposure to BPA disturbs mammary gland histoarchitecture and increases thecarcinogenic susceptibility to chemical challenges administered long after BPA exposure.Our aim was to assess the effect of prenatal BPA exposure on mammary glandangiogenesis and steroid hormone pathways in virgin cycling rats. Pregnant Wistar ratswere exposed to either 25 or 250 μg/kg/day (25 and 250 BPA, respectively) or to vehicle.Female offspring were autopsied on postnatal day (PND) 50 or 110. Ovarian steroid serumlevels, the expression of steroid receptors and their co-regulators SRC-3 and SMRT in themammary gland, and angiogenesis were evaluated. At PND 50, all BPA-treated animalshad lower serum levels of progesterone, while estradiol levels remained unchanged. Thehigher dose of BPA increased mammary ERa and decreased SRC-3 expression at PND 50and PND 110. SMRT protein levels were similar among groups at PND 50, whereas atPND 110, animals exposed to 250 BPA showed a lower SMRT expression. Interestingly, inthe control and 25 BPA groups, SMRT increased from PND 50 to PND 110. At PND 50, anincreased vascular area associated with higher VEGF expression was observed in the 250BPA-treated rats. At PND 110, the vascular area was still increased, but VEGF expressionwas similar to that of control rats. The present results demonstrate that prenatal exposure toBPA alters the endocrine environment of the mammary gland and its angiogenic process.Increased angiogenesis and altered steroid hormone signals could explain the higherfrequency of pre-neoplastic lesions found later in life.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103199
Durando, Milena de Lourdes; Kass, Laura; Perdomo, Virginia; Bosquiazzo, Veronica Lis; Luque, Enrique Hugo; et al.; Prenatal exposure to bisphenol A promotes angiogenesis and alters steroid-mediated responses in the mammary glands of cycling rats; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 127; 1-2; 10-2011; 35-43
0960-0760
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103199
identifier_str_mv Durando, Milena de Lourdes; Kass, Laura; Perdomo, Virginia; Bosquiazzo, Veronica Lis; Luque, Enrique Hugo; et al.; Prenatal exposure to bisphenol A promotes angiogenesis and alters steroid-mediated responses in the mammary glands of cycling rats; Pergamon-Elsevier Science Ltd; Journal of Steroid Biochemistry and Molecular Biology; 127; 1-2; 10-2011; 35-43
0960-0760
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0960076011000823
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jsbmb.2011.04.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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