Differential expression of disialic acids in the cerebellum of senile mice

Autores
Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Hidalgo, Alejandra Mabel; Loresi, Monica Alejandra; Argibay, Pablo F.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It is known that disialic acids (diSia) are present in the mammalian brain. However, the precise anatomical distribution and the chronology of its expression along life are not well studied yet. It is accepted that the transfer of diSia in the brain is mediated mainly by the enzyme ST8Sia III (α2,8-sialyltransferase III). We studied the expression of diSia glycoepitopes and of the ST8Sia III gene in different structures of the mouse brain at different postnatal stages by immunohistochemistry and real-time polymerase chain reaction, respectively. C57BL/6 mice of different stages were used. Samples of hippocampus, olfactory bulb, cortex and cerebellum were processed for studies of molecular biology and immunohistochemistry. Histological analysis revealed an important decrease in diSia labeling in the senile cerebellum compared with other structures and stages (P ≪ 0.001). In concordance with these results, a significant decrease in ST8Sia III gene expression was found in the cerebellum of senile animals (P < 0.001). These results suggest that diSia are constantly expressed but with differential expression in various areas of the mouse central nervous system. On the other hand, the concordance in the decreased expression of ST8Sia III and the diSia epitope in the cerebellum of senile animals suggest a role of diSia in this structure or, inversely, an influence of aging on the expression of diSia in the cerebellum. Further research in that direction could elucidate the roles of diSia in brain function in health and disease.
Fil: Rinflerch, Adriana Raquel. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Burgos, Valeria Laura. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hidalgo, Alejandra Mabel. Hospital Italiano; Argentina
Fil: Loresi, Monica Alejandra. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Argibay, Pablo F.. Hospital Italiano; Argentina
Materia
BRAIN
CEREBELLUM
DISIALIC ACID
SENILE
ST8SIA III
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194718

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network_name_str CONICET Digital (CONICET)
spelling Differential expression of disialic acids in the cerebellum of senile miceRinflerch, Adriana RaquelBurgos, Valeria LauraHidalgo, Alejandra MabelLoresi, Monica AlejandraArgibay, Pablo F.BRAINCEREBELLUMDISIALIC ACIDSENILEST8SIA IIIhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3It is known that disialic acids (diSia) are present in the mammalian brain. However, the precise anatomical distribution and the chronology of its expression along life are not well studied yet. It is accepted that the transfer of diSia in the brain is mediated mainly by the enzyme ST8Sia III (α2,8-sialyltransferase III). We studied the expression of diSia glycoepitopes and of the ST8Sia III gene in different structures of the mouse brain at different postnatal stages by immunohistochemistry and real-time polymerase chain reaction, respectively. C57BL/6 mice of different stages were used. Samples of hippocampus, olfactory bulb, cortex and cerebellum were processed for studies of molecular biology and immunohistochemistry. Histological analysis revealed an important decrease in diSia labeling in the senile cerebellum compared with other structures and stages (P ≪ 0.001). In concordance with these results, a significant decrease in ST8Sia III gene expression was found in the cerebellum of senile animals (P < 0.001). These results suggest that diSia are constantly expressed but with differential expression in various areas of the mouse central nervous system. On the other hand, the concordance in the decreased expression of ST8Sia III and the diSia epitope in the cerebellum of senile animals suggest a role of diSia in this structure or, inversely, an influence of aging on the expression of diSia in the cerebellum. Further research in that direction could elucidate the roles of diSia in brain function in health and disease.Fil: Rinflerch, Adriana Raquel. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Burgos, Valeria Laura. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hidalgo, Alejandra Mabel. Hospital Italiano; ArgentinaFil: Loresi, Monica Alejandra. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Argibay, Pablo F.. Hospital Italiano; ArgentinaOxford Univ Press Inc2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194718Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Hidalgo, Alejandra Mabel; Loresi, Monica Alejandra; Argibay, Pablo F.; Differential expression of disialic acids in the cerebellum of senile mice; Oxford Univ Press Inc; Glycobiology; 22; 3; 3-2012; 411-4160959-6658CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/glycob/article/22/3/411/2005013info:eu-repo/semantics/altIdentifier/doi/110.1093/glycob/cwr161info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:55Zoai:ri.conicet.gov.ar:11336/194718instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:55.605CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential expression of disialic acids in the cerebellum of senile mice
title Differential expression of disialic acids in the cerebellum of senile mice
spellingShingle Differential expression of disialic acids in the cerebellum of senile mice
Rinflerch, Adriana Raquel
BRAIN
CEREBELLUM
DISIALIC ACID
SENILE
ST8SIA III
title_short Differential expression of disialic acids in the cerebellum of senile mice
title_full Differential expression of disialic acids in the cerebellum of senile mice
title_fullStr Differential expression of disialic acids in the cerebellum of senile mice
title_full_unstemmed Differential expression of disialic acids in the cerebellum of senile mice
title_sort Differential expression of disialic acids in the cerebellum of senile mice
dc.creator.none.fl_str_mv Rinflerch, Adriana Raquel
Burgos, Valeria Laura
Hidalgo, Alejandra Mabel
Loresi, Monica Alejandra
Argibay, Pablo F.
author Rinflerch, Adriana Raquel
author_facet Rinflerch, Adriana Raquel
Burgos, Valeria Laura
Hidalgo, Alejandra Mabel
Loresi, Monica Alejandra
Argibay, Pablo F.
author_role author
author2 Burgos, Valeria Laura
Hidalgo, Alejandra Mabel
Loresi, Monica Alejandra
Argibay, Pablo F.
author2_role author
author
author
author
dc.subject.none.fl_str_mv BRAIN
CEREBELLUM
DISIALIC ACID
SENILE
ST8SIA III
topic BRAIN
CEREBELLUM
DISIALIC ACID
SENILE
ST8SIA III
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv It is known that disialic acids (diSia) are present in the mammalian brain. However, the precise anatomical distribution and the chronology of its expression along life are not well studied yet. It is accepted that the transfer of diSia in the brain is mediated mainly by the enzyme ST8Sia III (α2,8-sialyltransferase III). We studied the expression of diSia glycoepitopes and of the ST8Sia III gene in different structures of the mouse brain at different postnatal stages by immunohistochemistry and real-time polymerase chain reaction, respectively. C57BL/6 mice of different stages were used. Samples of hippocampus, olfactory bulb, cortex and cerebellum were processed for studies of molecular biology and immunohistochemistry. Histological analysis revealed an important decrease in diSia labeling in the senile cerebellum compared with other structures and stages (P ≪ 0.001). In concordance with these results, a significant decrease in ST8Sia III gene expression was found in the cerebellum of senile animals (P < 0.001). These results suggest that diSia are constantly expressed but with differential expression in various areas of the mouse central nervous system. On the other hand, the concordance in the decreased expression of ST8Sia III and the diSia epitope in the cerebellum of senile animals suggest a role of diSia in this structure or, inversely, an influence of aging on the expression of diSia in the cerebellum. Further research in that direction could elucidate the roles of diSia in brain function in health and disease.
Fil: Rinflerch, Adriana Raquel. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Burgos, Valeria Laura. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hidalgo, Alejandra Mabel. Hospital Italiano; Argentina
Fil: Loresi, Monica Alejandra. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Argibay, Pablo F.. Hospital Italiano; Argentina
description It is known that disialic acids (diSia) are present in the mammalian brain. However, the precise anatomical distribution and the chronology of its expression along life are not well studied yet. It is accepted that the transfer of diSia in the brain is mediated mainly by the enzyme ST8Sia III (α2,8-sialyltransferase III). We studied the expression of diSia glycoepitopes and of the ST8Sia III gene in different structures of the mouse brain at different postnatal stages by immunohistochemistry and real-time polymerase chain reaction, respectively. C57BL/6 mice of different stages were used. Samples of hippocampus, olfactory bulb, cortex and cerebellum were processed for studies of molecular biology and immunohistochemistry. Histological analysis revealed an important decrease in diSia labeling in the senile cerebellum compared with other structures and stages (P ≪ 0.001). In concordance with these results, a significant decrease in ST8Sia III gene expression was found in the cerebellum of senile animals (P < 0.001). These results suggest that diSia are constantly expressed but with differential expression in various areas of the mouse central nervous system. On the other hand, the concordance in the decreased expression of ST8Sia III and the diSia epitope in the cerebellum of senile animals suggest a role of diSia in this structure or, inversely, an influence of aging on the expression of diSia in the cerebellum. Further research in that direction could elucidate the roles of diSia in brain function in health and disease.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194718
Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Hidalgo, Alejandra Mabel; Loresi, Monica Alejandra; Argibay, Pablo F.; Differential expression of disialic acids in the cerebellum of senile mice; Oxford Univ Press Inc; Glycobiology; 22; 3; 3-2012; 411-416
0959-6658
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194718
identifier_str_mv Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Hidalgo, Alejandra Mabel; Loresi, Monica Alejandra; Argibay, Pablo F.; Differential expression of disialic acids in the cerebellum of senile mice; Oxford Univ Press Inc; Glycobiology; 22; 3; 3-2012; 411-416
0959-6658
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/glycob/article/22/3/411/2005013
info:eu-repo/semantics/altIdentifier/doi/110.1093/glycob/cwr161
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford Univ Press Inc
publisher.none.fl_str_mv Oxford Univ Press Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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