Differential expression of disialic acids in the cerebellum of senile mice
- Autores
- Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Hidalgo, Alejandra Mabel; Loresi, Monica Alejandra; Argibay, Pablo F.
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- It is known that disialic acids (diSia) are present in the mammalian brain. However, the precise anatomical distribution and the chronology of its expression along life are not well studied yet. It is accepted that the transfer of diSia in the brain is mediated mainly by the enzyme ST8Sia III (α2,8-sialyltransferase III). We studied the expression of diSia glycoepitopes and of the ST8Sia III gene in different structures of the mouse brain at different postnatal stages by immunohistochemistry and real-time polymerase chain reaction, respectively. C57BL/6 mice of different stages were used. Samples of hippocampus, olfactory bulb, cortex and cerebellum were processed for studies of molecular biology and immunohistochemistry. Histological analysis revealed an important decrease in diSia labeling in the senile cerebellum compared with other structures and stages (P ≪ 0.001). In concordance with these results, a significant decrease in ST8Sia III gene expression was found in the cerebellum of senile animals (P < 0.001). These results suggest that diSia are constantly expressed but with differential expression in various areas of the mouse central nervous system. On the other hand, the concordance in the decreased expression of ST8Sia III and the diSia epitope in the cerebellum of senile animals suggest a role of diSia in this structure or, inversely, an influence of aging on the expression of diSia in the cerebellum. Further research in that direction could elucidate the roles of diSia in brain function in health and disease.
Fil: Rinflerch, Adriana Raquel. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Burgos, Valeria Laura. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hidalgo, Alejandra Mabel. Hospital Italiano; Argentina
Fil: Loresi, Monica Alejandra. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Argibay, Pablo F.. Hospital Italiano; Argentina - Materia
-
BRAIN
CEREBELLUM
DISIALIC ACID
SENILE
ST8SIA III - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/194718
Ver los metadatos del registro completo
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Differential expression of disialic acids in the cerebellum of senile miceRinflerch, Adriana RaquelBurgos, Valeria LauraHidalgo, Alejandra MabelLoresi, Monica AlejandraArgibay, Pablo F.BRAINCEREBELLUMDISIALIC ACIDSENILEST8SIA IIIhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3It is known that disialic acids (diSia) are present in the mammalian brain. However, the precise anatomical distribution and the chronology of its expression along life are not well studied yet. It is accepted that the transfer of diSia in the brain is mediated mainly by the enzyme ST8Sia III (α2,8-sialyltransferase III). We studied the expression of diSia glycoepitopes and of the ST8Sia III gene in different structures of the mouse brain at different postnatal stages by immunohistochemistry and real-time polymerase chain reaction, respectively. C57BL/6 mice of different stages were used. Samples of hippocampus, olfactory bulb, cortex and cerebellum were processed for studies of molecular biology and immunohistochemistry. Histological analysis revealed an important decrease in diSia labeling in the senile cerebellum compared with other structures and stages (P ≪ 0.001). In concordance with these results, a significant decrease in ST8Sia III gene expression was found in the cerebellum of senile animals (P < 0.001). These results suggest that diSia are constantly expressed but with differential expression in various areas of the mouse central nervous system. On the other hand, the concordance in the decreased expression of ST8Sia III and the diSia epitope in the cerebellum of senile animals suggest a role of diSia in this structure or, inversely, an influence of aging on the expression of diSia in the cerebellum. Further research in that direction could elucidate the roles of diSia in brain function in health and disease.Fil: Rinflerch, Adriana Raquel. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Burgos, Valeria Laura. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hidalgo, Alejandra Mabel. Hospital Italiano; ArgentinaFil: Loresi, Monica Alejandra. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Argibay, Pablo F.. Hospital Italiano; ArgentinaOxford Univ Press Inc2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194718Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Hidalgo, Alejandra Mabel; Loresi, Monica Alejandra; Argibay, Pablo F.; Differential expression of disialic acids in the cerebellum of senile mice; Oxford Univ Press Inc; Glycobiology; 22; 3; 3-2012; 411-4160959-6658CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/glycob/article/22/3/411/2005013info:eu-repo/semantics/altIdentifier/doi/110.1093/glycob/cwr161info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:12Zoai:ri.conicet.gov.ar:11336/194718instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:12.583CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Differential expression of disialic acids in the cerebellum of senile mice |
| title |
Differential expression of disialic acids in the cerebellum of senile mice |
| spellingShingle |
Differential expression of disialic acids in the cerebellum of senile mice Rinflerch, Adriana Raquel BRAIN CEREBELLUM DISIALIC ACID SENILE ST8SIA III |
| title_short |
Differential expression of disialic acids in the cerebellum of senile mice |
| title_full |
Differential expression of disialic acids in the cerebellum of senile mice |
| title_fullStr |
Differential expression of disialic acids in the cerebellum of senile mice |
| title_full_unstemmed |
Differential expression of disialic acids in the cerebellum of senile mice |
| title_sort |
Differential expression of disialic acids in the cerebellum of senile mice |
| dc.creator.none.fl_str_mv |
Rinflerch, Adriana Raquel Burgos, Valeria Laura Hidalgo, Alejandra Mabel Loresi, Monica Alejandra Argibay, Pablo F. |
| author |
Rinflerch, Adriana Raquel |
| author_facet |
Rinflerch, Adriana Raquel Burgos, Valeria Laura Hidalgo, Alejandra Mabel Loresi, Monica Alejandra Argibay, Pablo F. |
| author_role |
author |
| author2 |
Burgos, Valeria Laura Hidalgo, Alejandra Mabel Loresi, Monica Alejandra Argibay, Pablo F. |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
BRAIN CEREBELLUM DISIALIC ACID SENILE ST8SIA III |
| topic |
BRAIN CEREBELLUM DISIALIC ACID SENILE ST8SIA III |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
It is known that disialic acids (diSia) are present in the mammalian brain. However, the precise anatomical distribution and the chronology of its expression along life are not well studied yet. It is accepted that the transfer of diSia in the brain is mediated mainly by the enzyme ST8Sia III (α2,8-sialyltransferase III). We studied the expression of diSia glycoepitopes and of the ST8Sia III gene in different structures of the mouse brain at different postnatal stages by immunohistochemistry and real-time polymerase chain reaction, respectively. C57BL/6 mice of different stages were used. Samples of hippocampus, olfactory bulb, cortex and cerebellum were processed for studies of molecular biology and immunohistochemistry. Histological analysis revealed an important decrease in diSia labeling in the senile cerebellum compared with other structures and stages (P ≪ 0.001). In concordance with these results, a significant decrease in ST8Sia III gene expression was found in the cerebellum of senile animals (P < 0.001). These results suggest that diSia are constantly expressed but with differential expression in various areas of the mouse central nervous system. On the other hand, the concordance in the decreased expression of ST8Sia III and the diSia epitope in the cerebellum of senile animals suggest a role of diSia in this structure or, inversely, an influence of aging on the expression of diSia in the cerebellum. Further research in that direction could elucidate the roles of diSia in brain function in health and disease. Fil: Rinflerch, Adriana Raquel. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Burgos, Valeria Laura. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hidalgo, Alejandra Mabel. Hospital Italiano; Argentina Fil: Loresi, Monica Alejandra. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Argibay, Pablo F.. Hospital Italiano; Argentina |
| description |
It is known that disialic acids (diSia) are present in the mammalian brain. However, the precise anatomical distribution and the chronology of its expression along life are not well studied yet. It is accepted that the transfer of diSia in the brain is mediated mainly by the enzyme ST8Sia III (α2,8-sialyltransferase III). We studied the expression of diSia glycoepitopes and of the ST8Sia III gene in different structures of the mouse brain at different postnatal stages by immunohistochemistry and real-time polymerase chain reaction, respectively. C57BL/6 mice of different stages were used. Samples of hippocampus, olfactory bulb, cortex and cerebellum were processed for studies of molecular biology and immunohistochemistry. Histological analysis revealed an important decrease in diSia labeling in the senile cerebellum compared with other structures and stages (P ≪ 0.001). In concordance with these results, a significant decrease in ST8Sia III gene expression was found in the cerebellum of senile animals (P < 0.001). These results suggest that diSia are constantly expressed but with differential expression in various areas of the mouse central nervous system. On the other hand, the concordance in the decreased expression of ST8Sia III and the diSia epitope in the cerebellum of senile animals suggest a role of diSia in this structure or, inversely, an influence of aging on the expression of diSia in the cerebellum. Further research in that direction could elucidate the roles of diSia in brain function in health and disease. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012-03 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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http://hdl.handle.net/11336/194718 Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Hidalgo, Alejandra Mabel; Loresi, Monica Alejandra; Argibay, Pablo F.; Differential expression of disialic acids in the cerebellum of senile mice; Oxford Univ Press Inc; Glycobiology; 22; 3; 3-2012; 411-416 0959-6658 CONICET Digital CONICET |
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http://hdl.handle.net/11336/194718 |
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Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Hidalgo, Alejandra Mabel; Loresi, Monica Alejandra; Argibay, Pablo F.; Differential expression of disialic acids in the cerebellum of senile mice; Oxford Univ Press Inc; Glycobiology; 22; 3; 3-2012; 411-416 0959-6658 CONICET Digital CONICET |
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eng |
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eng |
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Oxford Univ Press Inc |
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Oxford Univ Press Inc |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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