Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice
- Autores
- Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Ielpi, Marcelo; Ojea Quintana, Ignacio María; Hidalgo, Alejandra M.; Loresi, Monica Alejandra; Argibay, Pablo
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Several glycoproteins in mammalian brains contain α2,8-linked disialic acid residues. We previously showed a constant expression of disialic acid (DiSia) in the hippocampus, olfactory bulb and cortex, and a gradual decrease of expression in the cerebellum from neonatal to senile mice. Previous publications indicate that neurite extension of neuroblastoma-derived Neuro2A cells is inhibited in the presence of DiSia antibody. Based on this, we treated Neuro2A cell cultures with RNA interference for ST8SiaIII mRNA, the enzyme responsible for DiSia formation. We observed that neurite extension was inhibited by this treatment. Taking this evidence into consideration and the relationship of the cerebellum with learning and memory, we studied the role of DiSia expression in a learning task. Through delivery of pST8SiaIII into the brains of C57BL/6 neonatal mice, we inhibited the expression of ST8SiaIII. ST8SiaIII mRNA and protein expressions were analyzed by real-time PCR and western blot, respectively. In this work, we showed that pST8SiaIII-treated mice presented a significantly reduced level of ST8SiaIII mRNA in the cerebellum (p < 0.01) in comparison to control mice at 8 days after treatment. It is also noted that these levels returned to baseline values in the adulthood. Then, we evaluated behavioural performance in the T-Maze, a learning task that estimates procedural memory. At all ages, pST8SiaIII-treated mice showed a lower performance in the test session, being most evident at older ages (p < 0.001). Taken all together, we conclude that gene expression of ST8SiaIII is necessary for some cognitive tasks at early postnatal ages, since reduced levels impaired procedural memory in adult mice.
Fil: Rinflerch, Adriana Raquel. Hospital Italiano. Instituto de Cs.basicas y Medicina Experimental; Argentina
Fil: Burgos, Valeria Laura. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Ielpi, Marcelo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Ojea Quintana, Ignacio María. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Hidalgo, Alejandra M.. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Loresi, Monica Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Argibay, Pablo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Cerebellum
Disialic Acid
St8siaiii
Sialyltransferase - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/3642
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Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in miceRinflerch, Adriana RaquelBurgos, Valeria LauraIelpi, MarceloOjea Quintana, Ignacio MaríaHidalgo, Alejandra M.Loresi, Monica AlejandraArgibay, PabloCerebellumDisialic AcidSt8siaiiiSialyltransferasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Several glycoproteins in mammalian brains contain α2,8-linked disialic acid residues. We previously showed a constant expression of disialic acid (DiSia) in the hippocampus, olfactory bulb and cortex, and a gradual decrease of expression in the cerebellum from neonatal to senile mice. Previous publications indicate that neurite extension of neuroblastoma-derived Neuro2A cells is inhibited in the presence of DiSia antibody. Based on this, we treated Neuro2A cell cultures with RNA interference for ST8SiaIII mRNA, the enzyme responsible for DiSia formation. We observed that neurite extension was inhibited by this treatment. Taking this evidence into consideration and the relationship of the cerebellum with learning and memory, we studied the role of DiSia expression in a learning task. Through delivery of pST8SiaIII into the brains of C57BL/6 neonatal mice, we inhibited the expression of ST8SiaIII. ST8SiaIII mRNA and protein expressions were analyzed by real-time PCR and western blot, respectively. In this work, we showed that pST8SiaIII-treated mice presented a significantly reduced level of ST8SiaIII mRNA in the cerebellum (p < 0.01) in comparison to control mice at 8 days after treatment. It is also noted that these levels returned to baseline values in the adulthood. Then, we evaluated behavioural performance in the T-Maze, a learning task that estimates procedural memory. At all ages, pST8SiaIII-treated mice showed a lower performance in the test session, being most evident at older ages (p < 0.001). Taken all together, we conclude that gene expression of ST8SiaIII is necessary for some cognitive tasks at early postnatal ages, since reduced levels impaired procedural memory in adult mice.Fil: Rinflerch, Adriana Raquel. Hospital Italiano. Instituto de Cs.basicas y Medicina Experimental; ArgentinaFil: Burgos, Valeria Laura. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Ielpi, Marcelo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Ojea Quintana, Ignacio María. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Hidalgo, Alejandra M.. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Loresi, Monica Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Argibay, Pablo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2013-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3642Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Ielpi, Marcelo; Ojea Quintana, Ignacio María; Hidalgo, Alejandra M.; et al.; Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice; Elsevier; Neurochemistry International; 63; 5; 7-8-2013; 397-4040197-0186enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0197018613002088info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuint.2013.07.013info:eu-repo/semantics/altIdentifier/issn/0197-0186info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:48Zoai:ri.conicet.gov.ar:11336/3642instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:48.782CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice |
title |
Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice |
spellingShingle |
Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice Rinflerch, Adriana Raquel Cerebellum Disialic Acid St8siaiii Sialyltransferase |
title_short |
Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice |
title_full |
Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice |
title_fullStr |
Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice |
title_full_unstemmed |
Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice |
title_sort |
Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice |
dc.creator.none.fl_str_mv |
Rinflerch, Adriana Raquel Burgos, Valeria Laura Ielpi, Marcelo Ojea Quintana, Ignacio María Hidalgo, Alejandra M. Loresi, Monica Alejandra Argibay, Pablo |
author |
Rinflerch, Adriana Raquel |
author_facet |
Rinflerch, Adriana Raquel Burgos, Valeria Laura Ielpi, Marcelo Ojea Quintana, Ignacio María Hidalgo, Alejandra M. Loresi, Monica Alejandra Argibay, Pablo |
author_role |
author |
author2 |
Burgos, Valeria Laura Ielpi, Marcelo Ojea Quintana, Ignacio María Hidalgo, Alejandra M. Loresi, Monica Alejandra Argibay, Pablo |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Cerebellum Disialic Acid St8siaiii Sialyltransferase |
topic |
Cerebellum Disialic Acid St8siaiii Sialyltransferase |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Several glycoproteins in mammalian brains contain α2,8-linked disialic acid residues. We previously showed a constant expression of disialic acid (DiSia) in the hippocampus, olfactory bulb and cortex, and a gradual decrease of expression in the cerebellum from neonatal to senile mice. Previous publications indicate that neurite extension of neuroblastoma-derived Neuro2A cells is inhibited in the presence of DiSia antibody. Based on this, we treated Neuro2A cell cultures with RNA interference for ST8SiaIII mRNA, the enzyme responsible for DiSia formation. We observed that neurite extension was inhibited by this treatment. Taking this evidence into consideration and the relationship of the cerebellum with learning and memory, we studied the role of DiSia expression in a learning task. Through delivery of pST8SiaIII into the brains of C57BL/6 neonatal mice, we inhibited the expression of ST8SiaIII. ST8SiaIII mRNA and protein expressions were analyzed by real-time PCR and western blot, respectively. In this work, we showed that pST8SiaIII-treated mice presented a significantly reduced level of ST8SiaIII mRNA in the cerebellum (p < 0.01) in comparison to control mice at 8 days after treatment. It is also noted that these levels returned to baseline values in the adulthood. Then, we evaluated behavioural performance in the T-Maze, a learning task that estimates procedural memory. At all ages, pST8SiaIII-treated mice showed a lower performance in the test session, being most evident at older ages (p < 0.001). Taken all together, we conclude that gene expression of ST8SiaIII is necessary for some cognitive tasks at early postnatal ages, since reduced levels impaired procedural memory in adult mice. Fil: Rinflerch, Adriana Raquel. Hospital Italiano. Instituto de Cs.basicas y Medicina Experimental; Argentina Fil: Burgos, Valeria Laura. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina Fil: Ielpi, Marcelo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina Fil: Ojea Quintana, Ignacio María. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina Fil: Hidalgo, Alejandra M.. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina Fil: Loresi, Monica Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina Fil: Argibay, Pablo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Several glycoproteins in mammalian brains contain α2,8-linked disialic acid residues. We previously showed a constant expression of disialic acid (DiSia) in the hippocampus, olfactory bulb and cortex, and a gradual decrease of expression in the cerebellum from neonatal to senile mice. Previous publications indicate that neurite extension of neuroblastoma-derived Neuro2A cells is inhibited in the presence of DiSia antibody. Based on this, we treated Neuro2A cell cultures with RNA interference for ST8SiaIII mRNA, the enzyme responsible for DiSia formation. We observed that neurite extension was inhibited by this treatment. Taking this evidence into consideration and the relationship of the cerebellum with learning and memory, we studied the role of DiSia expression in a learning task. Through delivery of pST8SiaIII into the brains of C57BL/6 neonatal mice, we inhibited the expression of ST8SiaIII. ST8SiaIII mRNA and protein expressions were analyzed by real-time PCR and western blot, respectively. In this work, we showed that pST8SiaIII-treated mice presented a significantly reduced level of ST8SiaIII mRNA in the cerebellum (p < 0.01) in comparison to control mice at 8 days after treatment. It is also noted that these levels returned to baseline values in the adulthood. Then, we evaluated behavioural performance in the T-Maze, a learning task that estimates procedural memory. At all ages, pST8SiaIII-treated mice showed a lower performance in the test session, being most evident at older ages (p < 0.001). Taken all together, we conclude that gene expression of ST8SiaIII is necessary for some cognitive tasks at early postnatal ages, since reduced levels impaired procedural memory in adult mice. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/3642 Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Ielpi, Marcelo; Ojea Quintana, Ignacio María; Hidalgo, Alejandra M.; et al.; Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice; Elsevier; Neurochemistry International; 63; 5; 7-8-2013; 397-404 0197-0186 |
url |
http://hdl.handle.net/11336/3642 |
identifier_str_mv |
Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Ielpi, Marcelo; Ojea Quintana, Ignacio María; Hidalgo, Alejandra M.; et al.; Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice; Elsevier; Neurochemistry International; 63; 5; 7-8-2013; 397-404 0197-0186 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0197018613002088 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuint.2013.07.013 info:eu-repo/semantics/altIdentifier/issn/0197-0186 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268818029674496 |
score |
13.13397 |