Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice

Autores
Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Ielpi, Marcelo; Ojea Quintana, Ignacio María; Hidalgo, Alejandra M.; Loresi, Monica Alejandra; Argibay, Pablo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several glycoproteins in mammalian brains contain α2,8-linked disialic acid residues. We previously showed a constant expression of disialic acid (DiSia) in the hippocampus, olfactory bulb and cortex, and a gradual decrease of expression in the cerebellum from neonatal to senile mice. Previous publications indicate that neurite extension of neuroblastoma-derived Neuro2A cells is inhibited in the presence of DiSia antibody. Based on this, we treated Neuro2A cell cultures with RNA interference for ST8SiaIII mRNA, the enzyme responsible for DiSia formation. We observed that neurite extension was inhibited by this treatment. Taking this evidence into consideration and the relationship of the cerebellum with learning and memory, we studied the role of DiSia expression in a learning task. Through delivery of pST8SiaIII into the brains of C57BL/6 neonatal mice, we inhibited the expression of ST8SiaIII. ST8SiaIII mRNA and protein expressions were analyzed by real-time PCR and western blot, respectively. In this work, we showed that pST8SiaIII-treated mice presented a significantly reduced level of ST8SiaIII mRNA in the cerebellum (p < 0.01) in comparison to control mice at 8 days after treatment. It is also noted that these levels returned to baseline values in the adulthood. Then, we evaluated behavioural performance in the T-Maze, a learning task that estimates procedural memory. At all ages, pST8SiaIII-treated mice showed a lower performance in the test session, being most evident at older ages (p < 0.001). Taken all together, we conclude that gene expression of ST8SiaIII is necessary for some cognitive tasks at early postnatal ages, since reduced levels impaired procedural memory in adult mice.
Fil: Rinflerch, Adriana Raquel. Hospital Italiano. Instituto de Cs.basicas y Medicina Experimental; Argentina
Fil: Burgos, Valeria Laura. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Ielpi, Marcelo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Ojea Quintana, Ignacio María. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Hidalgo, Alejandra M.. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Loresi, Monica Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Argibay, Pablo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Cerebellum
Disialic Acid
St8siaiii
Sialyltransferase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3642
Acceder
id CONICETDig_ca57f5c43ad8e20ba546ff9b5350a742
oai_identifier_str oai:ri.conicet.gov.ar:11336/3642
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in miceRinflerch, Adriana RaquelBurgos, Valeria LauraIelpi, MarceloOjea Quintana, Ignacio MaríaHidalgo, Alejandra M.Loresi, Monica AlejandraArgibay, PabloCerebellumDisialic AcidSt8siaiiiSialyltransferasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Several glycoproteins in mammalian brains contain α2,8-linked disialic acid residues. We previously showed a constant expression of disialic acid (DiSia) in the hippocampus, olfactory bulb and cortex, and a gradual decrease of expression in the cerebellum from neonatal to senile mice. Previous publications indicate that neurite extension of neuroblastoma-derived Neuro2A cells is inhibited in the presence of DiSia antibody. Based on this, we treated Neuro2A cell cultures with RNA interference for ST8SiaIII mRNA, the enzyme responsible for DiSia formation. We observed that neurite extension was inhibited by this treatment. Taking this evidence into consideration and the relationship of the cerebellum with learning and memory, we studied the role of DiSia expression in a learning task. Through delivery of pST8SiaIII into the brains of C57BL/6 neonatal mice, we inhibited the expression of ST8SiaIII. ST8SiaIII mRNA and protein expressions were analyzed by real-time PCR and western blot, respectively. In this work, we showed that pST8SiaIII-treated mice presented a significantly reduced level of ST8SiaIII mRNA in the cerebellum (p < 0.01) in comparison to control mice at 8 days after treatment. It is also noted that these levels returned to baseline values in the adulthood. Then, we evaluated behavioural performance in the T-Maze, a learning task that estimates procedural memory. At all ages, pST8SiaIII-treated mice showed a lower performance in the test session, being most evident at older ages (p < 0.001). Taken all together, we conclude that gene expression of ST8SiaIII is necessary for some cognitive tasks at early postnatal ages, since reduced levels impaired procedural memory in adult mice.Fil: Rinflerch, Adriana Raquel. Hospital Italiano. Instituto de Cs.basicas y Medicina Experimental; ArgentinaFil: Burgos, Valeria Laura. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Ielpi, Marcelo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Ojea Quintana, Ignacio María. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Hidalgo, Alejandra M.. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Loresi, Monica Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; ArgentinaFil: Argibay, Pablo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2013-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3642Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Ielpi, Marcelo; Ojea Quintana, Ignacio María; Hidalgo, Alejandra M.; et al.; Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice; Elsevier; Neurochemistry International; 63; 5; 7-8-2013; 397-4040197-0186enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0197018613002088info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuint.2013.07.013info:eu-repo/semantics/altIdentifier/issn/0197-0186info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:17Zoai:ri.conicet.gov.ar:11336/3642instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:17.282CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice
title Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice
spellingShingle Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice
Rinflerch, Adriana Raquel
Cerebellum
Disialic Acid
St8siaiii
Sialyltransferase
title_short Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice
title_full Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice
title_fullStr Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice
title_full_unstemmed Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice
title_sort Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice
dc.creator.none.fl_str_mv Rinflerch, Adriana Raquel
Burgos, Valeria Laura
Ielpi, Marcelo
Ojea Quintana, Ignacio María
Hidalgo, Alejandra M.
Loresi, Monica Alejandra
Argibay, Pablo
author Rinflerch, Adriana Raquel
author_facet Rinflerch, Adriana Raquel
Burgos, Valeria Laura
Ielpi, Marcelo
Ojea Quintana, Ignacio María
Hidalgo, Alejandra M.
Loresi, Monica Alejandra
Argibay, Pablo
author_role author
author2 Burgos, Valeria Laura
Ielpi, Marcelo
Ojea Quintana, Ignacio María
Hidalgo, Alejandra M.
Loresi, Monica Alejandra
Argibay, Pablo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cerebellum
Disialic Acid
St8siaiii
Sialyltransferase
topic Cerebellum
Disialic Acid
St8siaiii
Sialyltransferase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Several glycoproteins in mammalian brains contain α2,8-linked disialic acid residues. We previously showed a constant expression of disialic acid (DiSia) in the hippocampus, olfactory bulb and cortex, and a gradual decrease of expression in the cerebellum from neonatal to senile mice. Previous publications indicate that neurite extension of neuroblastoma-derived Neuro2A cells is inhibited in the presence of DiSia antibody. Based on this, we treated Neuro2A cell cultures with RNA interference for ST8SiaIII mRNA, the enzyme responsible for DiSia formation. We observed that neurite extension was inhibited by this treatment. Taking this evidence into consideration and the relationship of the cerebellum with learning and memory, we studied the role of DiSia expression in a learning task. Through delivery of pST8SiaIII into the brains of C57BL/6 neonatal mice, we inhibited the expression of ST8SiaIII. ST8SiaIII mRNA and protein expressions were analyzed by real-time PCR and western blot, respectively. In this work, we showed that pST8SiaIII-treated mice presented a significantly reduced level of ST8SiaIII mRNA in the cerebellum (p < 0.01) in comparison to control mice at 8 days after treatment. It is also noted that these levels returned to baseline values in the adulthood. Then, we evaluated behavioural performance in the T-Maze, a learning task that estimates procedural memory. At all ages, pST8SiaIII-treated mice showed a lower performance in the test session, being most evident at older ages (p < 0.001). Taken all together, we conclude that gene expression of ST8SiaIII is necessary for some cognitive tasks at early postnatal ages, since reduced levels impaired procedural memory in adult mice.
Fil: Rinflerch, Adriana Raquel. Hospital Italiano. Instituto de Cs.basicas y Medicina Experimental; Argentina
Fil: Burgos, Valeria Laura. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Ielpi, Marcelo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Ojea Quintana, Ignacio María. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Hidalgo, Alejandra M.. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Loresi, Monica Alejandra. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina
Fil: Argibay, Pablo. Hospital Italiano. Instituto de Ciencias Básicas y Medicina Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Several glycoproteins in mammalian brains contain α2,8-linked disialic acid residues. We previously showed a constant expression of disialic acid (DiSia) in the hippocampus, olfactory bulb and cortex, and a gradual decrease of expression in the cerebellum from neonatal to senile mice. Previous publications indicate that neurite extension of neuroblastoma-derived Neuro2A cells is inhibited in the presence of DiSia antibody. Based on this, we treated Neuro2A cell cultures with RNA interference for ST8SiaIII mRNA, the enzyme responsible for DiSia formation. We observed that neurite extension was inhibited by this treatment. Taking this evidence into consideration and the relationship of the cerebellum with learning and memory, we studied the role of DiSia expression in a learning task. Through delivery of pST8SiaIII into the brains of C57BL/6 neonatal mice, we inhibited the expression of ST8SiaIII. ST8SiaIII mRNA and protein expressions were analyzed by real-time PCR and western blot, respectively. In this work, we showed that pST8SiaIII-treated mice presented a significantly reduced level of ST8SiaIII mRNA in the cerebellum (p < 0.01) in comparison to control mice at 8 days after treatment. It is also noted that these levels returned to baseline values in the adulthood. Then, we evaluated behavioural performance in the T-Maze, a learning task that estimates procedural memory. At all ages, pST8SiaIII-treated mice showed a lower performance in the test session, being most evident at older ages (p < 0.001). Taken all together, we conclude that gene expression of ST8SiaIII is necessary for some cognitive tasks at early postnatal ages, since reduced levels impaired procedural memory in adult mice.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3642
Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Ielpi, Marcelo; Ojea Quintana, Ignacio María; Hidalgo, Alejandra M.; et al.; Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice; Elsevier; Neurochemistry International; 63; 5; 7-8-2013; 397-404
0197-0186
url http://hdl.handle.net/11336/3642
identifier_str_mv Rinflerch, Adriana Raquel; Burgos, Valeria Laura; Ielpi, Marcelo; Ojea Quintana, Ignacio María; Hidalgo, Alejandra M.; et al.; Inhibition of brain ST8SiaIII sialyltransferase leads to impairment of procedural memory in mice; Elsevier; Neurochemistry International; 63; 5; 7-8-2013; 397-404
0197-0186
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0197018613002088
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuint.2013.07.013
info:eu-repo/semantics/altIdentifier/issn/0197-0186
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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