Temporal dissociation between sodium depletion and sodium appetite appearance: Involvement of inhibitory and stimulatory signals

Autores
Margatho, L. O.; Porcari, Cintia Yamila; Macchione, Ana Fabiola; da Silva Souza, G. D.; Caeiro, Ximena Elizabeth; Antunes Rodrigues, J.; Vivas, Laura Marta; Godino, Maria Andrea del Milagro
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Our aim was to analyze the participation of inhibitory and stimulatory signals in the temporal dissociation between sodium depletion (SD) induced by peritoneal dialysis (PD) and the appearance of sodium appetite (SA), particularly 2. h after PD, when the rats are hypovolemic/natremic but SA is not evident. We investigated the effects of bilateral injections of the serotonin (5-HT) receptor antagonist, methysergide, into the lateral parabrachial nucleus (LPBN) on hypertonic NaCl and water intake 2. h vs. 24. h after PD. We also studied plasma renin activity (PRA) and aldosterone (ALDO) concentration 2. h vs. 24. h after PD. Additionally, we combined the analysis of brain Fos immunoreactivity (Fos-ir) with the detection of double immunoreactivity in 5HT and oxytocinergic (OT) cells 2. h after PD. Bilateral LPBN injections of methysergide (4. μg/200. nl at each site) increased NaCl intake when tested 2. h after PD compared to controls. We found a significant increase in PRA and ALDO concentration after PD but no differences between 2 and 24. h after PD. We also found for the first time a significant increase 2. h after PD in the number of Fos-ir neurons in the brainstem nuclei that have been shown to be involved in the inhibition of SA.In summary, the results show that 5HT-mechanisms in the LPBN modulate sodium intake during the delay of SA when the renin angiotensin aldosterone system (RAAS) is increased. In addition, the activation of brainstem areas previously associated with the satiety phase of SA is in part responsible for the temporal dissociation between SD and behavioral arousal.
Fil: Margatho, L. O.. Universidade de Sao Paulo; Brasil
Fil: Porcari, Cintia Yamila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: da Silva Souza, G. D.. Universidade de Sao Paulo; Brasil
Fil: Caeiro, Ximena Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Antunes Rodrigues, J.. Universidade de Sao Paulo; Brasil
Fil: Vivas, Laura Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina
Fil: Godino, Maria Andrea del Milagro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Materia
Lateral Parabrachial Nucleus
Serotonergic System
Sodium Appetite
Sodium Depletion
Temporary Dissociation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50937
Acceder
id CONICETDig_443182a5be21d42419e39e8219e07b79
oai_identifier_str oai:ri.conicet.gov.ar:11336/50937
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Temporal dissociation between sodium depletion and sodium appetite appearance: Involvement of inhibitory and stimulatory signalsMargatho, L. O.Porcari, Cintia YamilaMacchione, Ana Fabiolada Silva Souza, G. D.Caeiro, Ximena ElizabethAntunes Rodrigues, J.Vivas, Laura MartaGodino, Maria Andrea del MilagroLateral Parabrachial NucleusSerotonergic SystemSodium AppetiteSodium DepletionTemporary Dissociationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Our aim was to analyze the participation of inhibitory and stimulatory signals in the temporal dissociation between sodium depletion (SD) induced by peritoneal dialysis (PD) and the appearance of sodium appetite (SA), particularly 2. h after PD, when the rats are hypovolemic/natremic but SA is not evident. We investigated the effects of bilateral injections of the serotonin (5-HT) receptor antagonist, methysergide, into the lateral parabrachial nucleus (LPBN) on hypertonic NaCl and water intake 2. h vs. 24. h after PD. We also studied plasma renin activity (PRA) and aldosterone (ALDO) concentration 2. h vs. 24. h after PD. Additionally, we combined the analysis of brain Fos immunoreactivity (Fos-ir) with the detection of double immunoreactivity in 5HT and oxytocinergic (OT) cells 2. h after PD. Bilateral LPBN injections of methysergide (4. μg/200. nl at each site) increased NaCl intake when tested 2. h after PD compared to controls. We found a significant increase in PRA and ALDO concentration after PD but no differences between 2 and 24. h after PD. We also found for the first time a significant increase 2. h after PD in the number of Fos-ir neurons in the brainstem nuclei that have been shown to be involved in the inhibition of SA.In summary, the results show that 5HT-mechanisms in the LPBN modulate sodium intake during the delay of SA when the renin angiotensin aldosterone system (RAAS) is increased. In addition, the activation of brainstem areas previously associated with the satiety phase of SA is in part responsible for the temporal dissociation between SD and behavioral arousal.Fil: Margatho, L. O.. Universidade de Sao Paulo; BrasilFil: Porcari, Cintia Yamila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: da Silva Souza, G. D.. Universidade de Sao Paulo; BrasilFil: Caeiro, Ximena Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Antunes Rodrigues, J.. Universidade de Sao Paulo; BrasilFil: Vivas, Laura Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Godino, Maria Andrea del Milagro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaPergamon-Elsevier Science Ltd2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50937Margatho, L. O.; Porcari, Cintia Yamila; Macchione, Ana Fabiola; da Silva Souza, G. D.; Caeiro, Ximena Elizabeth; et al.; Temporal dissociation between sodium depletion and sodium appetite appearance: Involvement of inhibitory and stimulatory signals; Pergamon-Elsevier Science Ltd; Neuroscience; 297; 6-2015; 78-880306-4522CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuroscience.2015.03.059info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0306452215003085info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:25Zoai:ri.conicet.gov.ar:11336/50937instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:25.796CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Temporal dissociation between sodium depletion and sodium appetite appearance: Involvement of inhibitory and stimulatory signals
title Temporal dissociation between sodium depletion and sodium appetite appearance: Involvement of inhibitory and stimulatory signals
spellingShingle Temporal dissociation between sodium depletion and sodium appetite appearance: Involvement of inhibitory and stimulatory signals
Margatho, L. O.
Lateral Parabrachial Nucleus
Serotonergic System
Sodium Appetite
Sodium Depletion
Temporary Dissociation
title_short Temporal dissociation between sodium depletion and sodium appetite appearance: Involvement of inhibitory and stimulatory signals
title_full Temporal dissociation between sodium depletion and sodium appetite appearance: Involvement of inhibitory and stimulatory signals
title_fullStr Temporal dissociation between sodium depletion and sodium appetite appearance: Involvement of inhibitory and stimulatory signals
title_full_unstemmed Temporal dissociation between sodium depletion and sodium appetite appearance: Involvement of inhibitory and stimulatory signals
title_sort Temporal dissociation between sodium depletion and sodium appetite appearance: Involvement of inhibitory and stimulatory signals
dc.creator.none.fl_str_mv Margatho, L. O.
Porcari, Cintia Yamila
Macchione, Ana Fabiola
da Silva Souza, G. D.
Caeiro, Ximena Elizabeth
Antunes Rodrigues, J.
Vivas, Laura Marta
Godino, Maria Andrea del Milagro
author Margatho, L. O.
author_facet Margatho, L. O.
Porcari, Cintia Yamila
Macchione, Ana Fabiola
da Silva Souza, G. D.
Caeiro, Ximena Elizabeth
Antunes Rodrigues, J.
Vivas, Laura Marta
Godino, Maria Andrea del Milagro
author_role author
author2 Porcari, Cintia Yamila
Macchione, Ana Fabiola
da Silva Souza, G. D.
Caeiro, Ximena Elizabeth
Antunes Rodrigues, J.
Vivas, Laura Marta
Godino, Maria Andrea del Milagro
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Lateral Parabrachial Nucleus
Serotonergic System
Sodium Appetite
Sodium Depletion
Temporary Dissociation
topic Lateral Parabrachial Nucleus
Serotonergic System
Sodium Appetite
Sodium Depletion
Temporary Dissociation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Our aim was to analyze the participation of inhibitory and stimulatory signals in the temporal dissociation between sodium depletion (SD) induced by peritoneal dialysis (PD) and the appearance of sodium appetite (SA), particularly 2. h after PD, when the rats are hypovolemic/natremic but SA is not evident. We investigated the effects of bilateral injections of the serotonin (5-HT) receptor antagonist, methysergide, into the lateral parabrachial nucleus (LPBN) on hypertonic NaCl and water intake 2. h vs. 24. h after PD. We also studied plasma renin activity (PRA) and aldosterone (ALDO) concentration 2. h vs. 24. h after PD. Additionally, we combined the analysis of brain Fos immunoreactivity (Fos-ir) with the detection of double immunoreactivity in 5HT and oxytocinergic (OT) cells 2. h after PD. Bilateral LPBN injections of methysergide (4. μg/200. nl at each site) increased NaCl intake when tested 2. h after PD compared to controls. We found a significant increase in PRA and ALDO concentration after PD but no differences between 2 and 24. h after PD. We also found for the first time a significant increase 2. h after PD in the number of Fos-ir neurons in the brainstem nuclei that have been shown to be involved in the inhibition of SA.In summary, the results show that 5HT-mechanisms in the LPBN modulate sodium intake during the delay of SA when the renin angiotensin aldosterone system (RAAS) is increased. In addition, the activation of brainstem areas previously associated with the satiety phase of SA is in part responsible for the temporal dissociation between SD and behavioral arousal.
Fil: Margatho, L. O.. Universidade de Sao Paulo; Brasil
Fil: Porcari, Cintia Yamila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: da Silva Souza, G. D.. Universidade de Sao Paulo; Brasil
Fil: Caeiro, Ximena Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Antunes Rodrigues, J.. Universidade de Sao Paulo; Brasil
Fil: Vivas, Laura Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina
Fil: Godino, Maria Andrea del Milagro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
description Our aim was to analyze the participation of inhibitory and stimulatory signals in the temporal dissociation between sodium depletion (SD) induced by peritoneal dialysis (PD) and the appearance of sodium appetite (SA), particularly 2. h after PD, when the rats are hypovolemic/natremic but SA is not evident. We investigated the effects of bilateral injections of the serotonin (5-HT) receptor antagonist, methysergide, into the lateral parabrachial nucleus (LPBN) on hypertonic NaCl and water intake 2. h vs. 24. h after PD. We also studied plasma renin activity (PRA) and aldosterone (ALDO) concentration 2. h vs. 24. h after PD. Additionally, we combined the analysis of brain Fos immunoreactivity (Fos-ir) with the detection of double immunoreactivity in 5HT and oxytocinergic (OT) cells 2. h after PD. Bilateral LPBN injections of methysergide (4. μg/200. nl at each site) increased NaCl intake when tested 2. h after PD compared to controls. We found a significant increase in PRA and ALDO concentration after PD but no differences between 2 and 24. h after PD. We also found for the first time a significant increase 2. h after PD in the number of Fos-ir neurons in the brainstem nuclei that have been shown to be involved in the inhibition of SA.In summary, the results show that 5HT-mechanisms in the LPBN modulate sodium intake during the delay of SA when the renin angiotensin aldosterone system (RAAS) is increased. In addition, the activation of brainstem areas previously associated with the satiety phase of SA is in part responsible for the temporal dissociation between SD and behavioral arousal.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50937
Margatho, L. O.; Porcari, Cintia Yamila; Macchione, Ana Fabiola; da Silva Souza, G. D.; Caeiro, Ximena Elizabeth; et al.; Temporal dissociation between sodium depletion and sodium appetite appearance: Involvement of inhibitory and stimulatory signals; Pergamon-Elsevier Science Ltd; Neuroscience; 297; 6-2015; 78-88
0306-4522
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50937
identifier_str_mv Margatho, L. O.; Porcari, Cintia Yamila; Macchione, Ana Fabiola; da Silva Souza, G. D.; Caeiro, Ximena Elizabeth; et al.; Temporal dissociation between sodium depletion and sodium appetite appearance: Involvement of inhibitory and stimulatory signals; Pergamon-Elsevier Science Ltd; Neuroscience; 297; 6-2015; 78-88
0306-4522
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuroscience.2015.03.059
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0306452215003085
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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