Serotonergic system involvement in the inhibitory action of estrogen on induced sodium appetite in female rats

Autores
Dalmasso, Carolina; Amigone, José Luis; Vivas, Laura Marta
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study of the participation of the serotonergic system in the inhibitory effect of estrogen on induced sodium appetite in female rats explores sodiumappetite induced by Furosemide and lowsodiumdiet treatment (DEP) in normally cycling rats and in ovariectomized rats with and without estradiol replacement (OVX, OVX+E2) .We also analyzed the neural activity of serotonergic neurons of the dorsal raphe nucleus (DRN) aswell as the activity of other brain nuclei previously found to be involved in sodium and water balance in sodium depleted animals without access to the intake test. For this purpose,we examined the brain Fos, Fos-serotonin and Fos-vasopressin immunoreactivity patterns in diestrus (D), estrus (E), OVX and OVX+E2 rats subjected to DEP. Female rats in E and OVX+E2 exhibited a significant decrease in induced sodiumintake comparedwith females in D and OVX. This estrogen-dependent inhibition on induced sodium appetite (approximately 50% reduction) can be correlated with changes in Fos activation observed in the organum vasculosum of the lamina terminalis (OVLT) and DRN, in response to sodium depletion. Given our previous observations in males, the expected sodium depletion-induced activity of the OVLT was found to be absent in OVX+E2 females, while the usual inhibitory tonic activity of serotonergic neurons of the DRN, instead of decreasing after sodium depletion, increases or remains unchanged in OVX+E2-DEP and E-DEP females, respectively. Regarding urinary water and sodium excretion 3 h after furosemide treatment, E-DEP and OVX+E2-DEP animals excreted smaller volumes of more highly concentrated urine than depleted D and OVX rats. Twenty hours after sodium depletion, the same groups of animals also showed a significant increase in the number of Fos-AVP immunoreactive neurons within the supraoptic nucleus, compared with D-DEP. In summary, our results demonstrate an estrogen-dependent inhibition of induced sodiumappetite in normally cycling rats and ovariectomized animals with estradiol replacement, whichmay involve an interaction between excitatory neurons of the OVLT and inhibitory serotonergic cells of the DRN. The main finding is thus serotonergic system involvement as a possible mechanism in the inhibitory action of estrogen on induced sodium appetite.
Fil: Dalmasso, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Amigone, José Luis. No especifíca;
Fil: Vivas, Laura Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Materia
SEROTONERGIC SYSTEM
SODIUM APPETITE
ESTROUS CYCLE
VASOPRESSINERGIC NEURONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281109
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/281109
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Serotonergic system involvement in the inhibitory action of estrogen on induced sodium appetite in female ratsDalmasso, CarolinaAmigone, José LuisVivas, Laura MartaSEROTONERGIC SYSTEMSODIUM APPETITEESTROUS CYCLEVASOPRESSINERGIC NEURONShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3This study of the participation of the serotonergic system in the inhibitory effect of estrogen on induced sodium appetite in female rats explores sodiumappetite induced by Furosemide and lowsodiumdiet treatment (DEP) in normally cycling rats and in ovariectomized rats with and without estradiol replacement (OVX, OVX+E2) .We also analyzed the neural activity of serotonergic neurons of the dorsal raphe nucleus (DRN) aswell as the activity of other brain nuclei previously found to be involved in sodium and water balance in sodium depleted animals without access to the intake test. For this purpose,we examined the brain Fos, Fos-serotonin and Fos-vasopressin immunoreactivity patterns in diestrus (D), estrus (E), OVX and OVX+E2 rats subjected to DEP. Female rats in E and OVX+E2 exhibited a significant decrease in induced sodiumintake comparedwith females in D and OVX. This estrogen-dependent inhibition on induced sodium appetite (approximately 50% reduction) can be correlated with changes in Fos activation observed in the organum vasculosum of the lamina terminalis (OVLT) and DRN, in response to sodium depletion. Given our previous observations in males, the expected sodium depletion-induced activity of the OVLT was found to be absent in OVX+E2 females, while the usual inhibitory tonic activity of serotonergic neurons of the DRN, instead of decreasing after sodium depletion, increases or remains unchanged in OVX+E2-DEP and E-DEP females, respectively. Regarding urinary water and sodium excretion 3 h after furosemide treatment, E-DEP and OVX+E2-DEP animals excreted smaller volumes of more highly concentrated urine than depleted D and OVX rats. Twenty hours after sodium depletion, the same groups of animals also showed a significant increase in the number of Fos-AVP immunoreactive neurons within the supraoptic nucleus, compared with D-DEP. In summary, our results demonstrate an estrogen-dependent inhibition of induced sodiumappetite in normally cycling rats and ovariectomized animals with estradiol replacement, whichmay involve an interaction between excitatory neurons of the OVLT and inhibitory serotonergic cells of the DRN. The main finding is thus serotonergic system involvement as a possible mechanism in the inhibitory action of estrogen on induced sodium appetite.Fil: Dalmasso, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Amigone, José Luis. No especifíca;Fil: Vivas, Laura Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaPergamon-Elsevier Science Ltd2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281109Dalmasso, Carolina; Amigone, José Luis; Vivas, Laura Marta; Serotonergic system involvement in the inhibitory action of estrogen on induced sodium appetite in female rats; Pergamon-Elsevier Science Ltd; Physiology And Behavior; 104; 3; 4-2011; 398-4070031-9384CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/10.1016/j.physbeh.2011.04.029info:eu-repo/semantics/altIdentifier/doi/10.1016/j.physbeh.2011.04.029info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:26:15Zoai:ri.conicet.gov.ar:11336/281109instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:26:15.441CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Serotonergic system involvement in the inhibitory action of estrogen on induced sodium appetite in female rats
title Serotonergic system involvement in the inhibitory action of estrogen on induced sodium appetite in female rats
spellingShingle Serotonergic system involvement in the inhibitory action of estrogen on induced sodium appetite in female rats
Dalmasso, Carolina
SEROTONERGIC SYSTEM
SODIUM APPETITE
ESTROUS CYCLE
VASOPRESSINERGIC NEURONS
title_short Serotonergic system involvement in the inhibitory action of estrogen on induced sodium appetite in female rats
title_full Serotonergic system involvement in the inhibitory action of estrogen on induced sodium appetite in female rats
title_fullStr Serotonergic system involvement in the inhibitory action of estrogen on induced sodium appetite in female rats
title_full_unstemmed Serotonergic system involvement in the inhibitory action of estrogen on induced sodium appetite in female rats
title_sort Serotonergic system involvement in the inhibitory action of estrogen on induced sodium appetite in female rats
dc.creator.none.fl_str_mv Dalmasso, Carolina
Amigone, José Luis
Vivas, Laura Marta
author Dalmasso, Carolina
author_facet Dalmasso, Carolina
Amigone, José Luis
Vivas, Laura Marta
author_role author
author2 Amigone, José Luis
Vivas, Laura Marta
author2_role author
author
dc.subject.none.fl_str_mv SEROTONERGIC SYSTEM
SODIUM APPETITE
ESTROUS CYCLE
VASOPRESSINERGIC NEURONS
topic SEROTONERGIC SYSTEM
SODIUM APPETITE
ESTROUS CYCLE
VASOPRESSINERGIC NEURONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv This study of the participation of the serotonergic system in the inhibitory effect of estrogen on induced sodium appetite in female rats explores sodiumappetite induced by Furosemide and lowsodiumdiet treatment (DEP) in normally cycling rats and in ovariectomized rats with and without estradiol replacement (OVX, OVX+E2) .We also analyzed the neural activity of serotonergic neurons of the dorsal raphe nucleus (DRN) aswell as the activity of other brain nuclei previously found to be involved in sodium and water balance in sodium depleted animals without access to the intake test. For this purpose,we examined the brain Fos, Fos-serotonin and Fos-vasopressin immunoreactivity patterns in diestrus (D), estrus (E), OVX and OVX+E2 rats subjected to DEP. Female rats in E and OVX+E2 exhibited a significant decrease in induced sodiumintake comparedwith females in D and OVX. This estrogen-dependent inhibition on induced sodium appetite (approximately 50% reduction) can be correlated with changes in Fos activation observed in the organum vasculosum of the lamina terminalis (OVLT) and DRN, in response to sodium depletion. Given our previous observations in males, the expected sodium depletion-induced activity of the OVLT was found to be absent in OVX+E2 females, while the usual inhibitory tonic activity of serotonergic neurons of the DRN, instead of decreasing after sodium depletion, increases or remains unchanged in OVX+E2-DEP and E-DEP females, respectively. Regarding urinary water and sodium excretion 3 h after furosemide treatment, E-DEP and OVX+E2-DEP animals excreted smaller volumes of more highly concentrated urine than depleted D and OVX rats. Twenty hours after sodium depletion, the same groups of animals also showed a significant increase in the number of Fos-AVP immunoreactive neurons within the supraoptic nucleus, compared with D-DEP. In summary, our results demonstrate an estrogen-dependent inhibition of induced sodiumappetite in normally cycling rats and ovariectomized animals with estradiol replacement, whichmay involve an interaction between excitatory neurons of the OVLT and inhibitory serotonergic cells of the DRN. The main finding is thus serotonergic system involvement as a possible mechanism in the inhibitory action of estrogen on induced sodium appetite.
Fil: Dalmasso, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Amigone, José Luis. No especifíca;
Fil: Vivas, Laura Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
description This study of the participation of the serotonergic system in the inhibitory effect of estrogen on induced sodium appetite in female rats explores sodiumappetite induced by Furosemide and lowsodiumdiet treatment (DEP) in normally cycling rats and in ovariectomized rats with and without estradiol replacement (OVX, OVX+E2) .We also analyzed the neural activity of serotonergic neurons of the dorsal raphe nucleus (DRN) aswell as the activity of other brain nuclei previously found to be involved in sodium and water balance in sodium depleted animals without access to the intake test. For this purpose,we examined the brain Fos, Fos-serotonin and Fos-vasopressin immunoreactivity patterns in diestrus (D), estrus (E), OVX and OVX+E2 rats subjected to DEP. Female rats in E and OVX+E2 exhibited a significant decrease in induced sodiumintake comparedwith females in D and OVX. This estrogen-dependent inhibition on induced sodium appetite (approximately 50% reduction) can be correlated with changes in Fos activation observed in the organum vasculosum of the lamina terminalis (OVLT) and DRN, in response to sodium depletion. Given our previous observations in males, the expected sodium depletion-induced activity of the OVLT was found to be absent in OVX+E2 females, while the usual inhibitory tonic activity of serotonergic neurons of the DRN, instead of decreasing after sodium depletion, increases or remains unchanged in OVX+E2-DEP and E-DEP females, respectively. Regarding urinary water and sodium excretion 3 h after furosemide treatment, E-DEP and OVX+E2-DEP animals excreted smaller volumes of more highly concentrated urine than depleted D and OVX rats. Twenty hours after sodium depletion, the same groups of animals also showed a significant increase in the number of Fos-AVP immunoreactive neurons within the supraoptic nucleus, compared with D-DEP. In summary, our results demonstrate an estrogen-dependent inhibition of induced sodiumappetite in normally cycling rats and ovariectomized animals with estradiol replacement, whichmay involve an interaction between excitatory neurons of the OVLT and inhibitory serotonergic cells of the DRN. The main finding is thus serotonergic system involvement as a possible mechanism in the inhibitory action of estrogen on induced sodium appetite.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281109
Dalmasso, Carolina; Amigone, José Luis; Vivas, Laura Marta; Serotonergic system involvement in the inhibitory action of estrogen on induced sodium appetite in female rats; Pergamon-Elsevier Science Ltd; Physiology And Behavior; 104; 3; 4-2011; 398-407
0031-9384
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281109
identifier_str_mv Dalmasso, Carolina; Amigone, José Luis; Vivas, Laura Marta; Serotonergic system involvement in the inhibitory action of estrogen on induced sodium appetite in female rats; Pergamon-Elsevier Science Ltd; Physiology And Behavior; 104; 3; 4-2011; 398-407
0031-9384
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/10.1016/j.physbeh.2011.04.029
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.physbeh.2011.04.029
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.176822

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