Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri ) by Electron Paramagnetic Spin Resonance Spectrometry

Autores
Díaz, Ana Cristina; Fernandez Gimenez, Analia Veronica; Mendiara, Sara Noemí; Fenucci, Jorge Lino
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Free radical scavenging properties of hepatopancreas extracts of Pleoticus muelleri were evaluated by electron paramagnetic spin resonance spectrometry methods (EPR) against the stable 1,1-diphenyl2-picrylhydrazyl (DPPH) radical. The present study was carried out to characterize different physiological stages of the shrimp under environmental and nutritional stress, evaluating the effect on growth, survival, and functional morphology of the hepatopancreas. Feeding trials were carried out on juveniles (1 g initial weight) held in aquaria. Each diet, with different concentrations of vitamins A and E, was tested in triplicate groups during 25 days. The control groups were fed with fresh squid mantle and with a vitamin-free diet. For all of the diets, the extracts exhibited strong DPPH radical scavenging activity, suggesting that the tissue is a powerful natural antioxidant. Individuals fed with different concentrations of vitamin E showed the strongest effect on the DPPH radicals, reducing the DPPH radicals to 50%, after an incubation period of 3 min. In contrast, the extracts of control animals, fed with squid mantle, had the weakest antioxidant activity (4%). These data indicated that the presence of vitamin E in the diet can provide immediate protection against free radicals.
Fil: Díaz, Ana Cristina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; Argentina
Fil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; Argentina
Fil: Mendiara, Sara Noemí. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; Argentina
Fil: Fenucci, Jorge Lino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; Argentina
Materia
ANTIOXIDANT
HEPATOPANCREAS
SHRIMP
ELECTRON PARAMAGNETIC SPIN RESONANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/240908
Acceder
id CONICETDig_43488e238fbcb9491e95d00b43e49e0f
oai_identifier_str oai:ri.conicet.gov.ar:11336/240908
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri ) by Electron Paramagnetic Spin Resonance SpectrometryDíaz, Ana CristinaFernandez Gimenez, Analia VeronicaMendiara, Sara NoemíFenucci, Jorge LinoANTIOXIDANTHEPATOPANCREASSHRIMPELECTRON PARAMAGNETIC SPIN RESONANCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Free radical scavenging properties of hepatopancreas extracts of Pleoticus muelleri were evaluated by electron paramagnetic spin resonance spectrometry methods (EPR) against the stable 1,1-diphenyl2-picrylhydrazyl (DPPH) radical. The present study was carried out to characterize different physiological stages of the shrimp under environmental and nutritional stress, evaluating the effect on growth, survival, and functional morphology of the hepatopancreas. Feeding trials were carried out on juveniles (1 g initial weight) held in aquaria. Each diet, with different concentrations of vitamins A and E, was tested in triplicate groups during 25 days. The control groups were fed with fresh squid mantle and with a vitamin-free diet. For all of the diets, the extracts exhibited strong DPPH radical scavenging activity, suggesting that the tissue is a powerful natural antioxidant. Individuals fed with different concentrations of vitamin E showed the strongest effect on the DPPH radicals, reducing the DPPH radicals to 50%, after an incubation period of 3 min. In contrast, the extracts of control animals, fed with squid mantle, had the weakest antioxidant activity (4%). These data indicated that the presence of vitamin E in the diet can provide immediate protection against free radicals.Fil: Díaz, Ana Cristina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; ArgentinaFil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; ArgentinaFil: Mendiara, Sara Noemí. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; ArgentinaFil: Fenucci, Jorge Lino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; ArgentinaAmerican Chemical Society2004-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/240908Díaz, Ana Cristina; Fernandez Gimenez, Analia Veronica; Mendiara, Sara Noemí; Fenucci, Jorge Lino; Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri ) by Electron Paramagnetic Spin Resonance Spectrometry; American Chemical Society; Journal of Agricultural and Food Chemistry; 52; 10; 4-2004; 3189-31930021-8561CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/jf0346962info:eu-repo/semantics/altIdentifier/doi/10.1021/jf0346962info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:03:19Zoai:ri.conicet.gov.ar:11336/240908instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:03:19.826CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri ) by Electron Paramagnetic Spin Resonance Spectrometry
title Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri ) by Electron Paramagnetic Spin Resonance Spectrometry
spellingShingle Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri ) by Electron Paramagnetic Spin Resonance Spectrometry
Díaz, Ana Cristina
ANTIOXIDANT
HEPATOPANCREAS
SHRIMP
ELECTRON PARAMAGNETIC SPIN RESONANCE
title_short Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri ) by Electron Paramagnetic Spin Resonance Spectrometry
title_full Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri ) by Electron Paramagnetic Spin Resonance Spectrometry
title_fullStr Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri ) by Electron Paramagnetic Spin Resonance Spectrometry
title_full_unstemmed Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri ) by Electron Paramagnetic Spin Resonance Spectrometry
title_sort Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri ) by Electron Paramagnetic Spin Resonance Spectrometry
dc.creator.none.fl_str_mv Díaz, Ana Cristina
Fernandez Gimenez, Analia Veronica
Mendiara, Sara Noemí
Fenucci, Jorge Lino
author Díaz, Ana Cristina
author_facet Díaz, Ana Cristina
Fernandez Gimenez, Analia Veronica
Mendiara, Sara Noemí
Fenucci, Jorge Lino
author_role author
author2 Fernandez Gimenez, Analia Veronica
Mendiara, Sara Noemí
Fenucci, Jorge Lino
author2_role author
author
author
dc.subject.none.fl_str_mv ANTIOXIDANT
HEPATOPANCREAS
SHRIMP
ELECTRON PARAMAGNETIC SPIN RESONANCE
topic ANTIOXIDANT
HEPATOPANCREAS
SHRIMP
ELECTRON PARAMAGNETIC SPIN RESONANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Free radical scavenging properties of hepatopancreas extracts of Pleoticus muelleri were evaluated by electron paramagnetic spin resonance spectrometry methods (EPR) against the stable 1,1-diphenyl2-picrylhydrazyl (DPPH) radical. The present study was carried out to characterize different physiological stages of the shrimp under environmental and nutritional stress, evaluating the effect on growth, survival, and functional morphology of the hepatopancreas. Feeding trials were carried out on juveniles (1 g initial weight) held in aquaria. Each diet, with different concentrations of vitamins A and E, was tested in triplicate groups during 25 days. The control groups were fed with fresh squid mantle and with a vitamin-free diet. For all of the diets, the extracts exhibited strong DPPH radical scavenging activity, suggesting that the tissue is a powerful natural antioxidant. Individuals fed with different concentrations of vitamin E showed the strongest effect on the DPPH radicals, reducing the DPPH radicals to 50%, after an incubation period of 3 min. In contrast, the extracts of control animals, fed with squid mantle, had the weakest antioxidant activity (4%). These data indicated that the presence of vitamin E in the diet can provide immediate protection against free radicals.
Fil: Díaz, Ana Cristina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; Argentina
Fil: Fernandez Gimenez, Analia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; Argentina
Fil: Mendiara, Sara Noemí. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; Argentina
Fil: Fenucci, Jorge Lino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; Argentina
description Free radical scavenging properties of hepatopancreas extracts of Pleoticus muelleri were evaluated by electron paramagnetic spin resonance spectrometry methods (EPR) against the stable 1,1-diphenyl2-picrylhydrazyl (DPPH) radical. The present study was carried out to characterize different physiological stages of the shrimp under environmental and nutritional stress, evaluating the effect on growth, survival, and functional morphology of the hepatopancreas. Feeding trials were carried out on juveniles (1 g initial weight) held in aquaria. Each diet, with different concentrations of vitamins A and E, was tested in triplicate groups during 25 days. The control groups were fed with fresh squid mantle and with a vitamin-free diet. For all of the diets, the extracts exhibited strong DPPH radical scavenging activity, suggesting that the tissue is a powerful natural antioxidant. Individuals fed with different concentrations of vitamin E showed the strongest effect on the DPPH radicals, reducing the DPPH radicals to 50%, after an incubation period of 3 min. In contrast, the extracts of control animals, fed with squid mantle, had the weakest antioxidant activity (4%). These data indicated that the presence of vitamin E in the diet can provide immediate protection against free radicals.
publishDate 2004
dc.date.none.fl_str_mv 2004-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/240908
Díaz, Ana Cristina; Fernandez Gimenez, Analia Veronica; Mendiara, Sara Noemí; Fenucci, Jorge Lino; Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri ) by Electron Paramagnetic Spin Resonance Spectrometry; American Chemical Society; Journal of Agricultural and Food Chemistry; 52; 10; 4-2004; 3189-3193
0021-8561
CONICET Digital
CONICET
url http://hdl.handle.net/11336/240908
identifier_str_mv Díaz, Ana Cristina; Fernandez Gimenez, Analia Veronica; Mendiara, Sara Noemí; Fenucci, Jorge Lino; Antioxidant Activity in Hepatopancreas of the Shrimp (Pleoticus muelleri ) by Electron Paramagnetic Spin Resonance Spectrometry; American Chemical Society; Journal of Agricultural and Food Chemistry; 52; 10; 4-2004; 3189-3193
0021-8561
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/jf0346962
info:eu-repo/semantics/altIdentifier/doi/10.1021/jf0346962
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980075465605120
score 12.993085

Publicaciones similares