Selection and Performance of Antifungal Lactic Acid Bacteria in Corn Mini-Silos

Autores
Carrizo, Natalí Ivana; Carabajal Torrez, José Agustín; Molina, Fatima Romina Elizabeth; Fornaguera, Maria Jose; Martos, Gladys Irma; Bustos, Ana Yanina; Gerez, Carla Luciana
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Silage is a method of forage conservation based on lactic fermentation. In order to improve the fermentative performance in corn silages, the aims of the present work were as follows: (I) to screen lactic acid bacteria with acidifying activity, (II) to study their antifungal activity against filamentous fungi and yeasts and (III) to evaluate their performance as antifungal in mini-silos. The acidifying activity was determined by measuring the pH and total titratable acidity. Those most acidifying bacteria were incubated in plates together with fungal markers strains to observe the presence or absence of inhibition halos, in addition to studying the nature of the antifungal metabolites by means of the treatment with proteases. The strain with the widest spectrum of action was selected to formulate an inoculum used in corn mini-silos. During storage, both silos determined humidity, acidification and microbiological composition, and then aerobic stability. The results showed that Lactoplantibacillus (L.) plantarum cultures had a higher production of lactic acid (7.7–17.5 g/L) than Pediococcus (8.8–10.9 g/L). L. plantarum CRL363 could inhibit the growth of filamentous fungi and some yeasts and consequently it was used in mini-silos. Compared to the control, the inoculated silo presented low pH values (4.02–4.85) and low fungi and yeast count until 20 days of storage, demonstrating the great potential of CRL363 strain for the formulation of inoculants for corn silage.
Fil: Carrizo, Natalí Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Carabajal Torrez, José Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Molina, Fatima Romina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Fornaguera, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Martos, Gladys Irma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Bustos, Ana Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Gerez, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
ANTIFUNGAL ACTIVITY
FERMENTATION
LACTIC ACID BACTERIA
SILAGE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184329
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/184329
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Selection and Performance of Antifungal Lactic Acid Bacteria in Corn Mini-SilosCarrizo, Natalí IvanaCarabajal Torrez, José AgustínMolina, Fatima Romina ElizabethFornaguera, Maria JoseMartos, Gladys IrmaBustos, Ana YaninaGerez, Carla LucianaANTIFUNGAL ACTIVITYFERMENTATIONLACTIC ACID BACTERIASILAGEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Silage is a method of forage conservation based on lactic fermentation. In order to improve the fermentative performance in corn silages, the aims of the present work were as follows: (I) to screen lactic acid bacteria with acidifying activity, (II) to study their antifungal activity against filamentous fungi and yeasts and (III) to evaluate their performance as antifungal in mini-silos. The acidifying activity was determined by measuring the pH and total titratable acidity. Those most acidifying bacteria were incubated in plates together with fungal markers strains to observe the presence or absence of inhibition halos, in addition to studying the nature of the antifungal metabolites by means of the treatment with proteases. The strain with the widest spectrum of action was selected to formulate an inoculum used in corn mini-silos. During storage, both silos determined humidity, acidification and microbiological composition, and then aerobic stability. The results showed that Lactoplantibacillus (L.) plantarum cultures had a higher production of lactic acid (7.7–17.5 g/L) than Pediococcus (8.8–10.9 g/L). L. plantarum CRL363 could inhibit the growth of filamentous fungi and some yeasts and consequently it was used in mini-silos. Compared to the control, the inoculated silo presented low pH values (4.02–4.85) and low fungi and yeast count until 20 days of storage, demonstrating the great potential of CRL363 strain for the formulation of inoculants for corn silage.Fil: Carrizo, Natalí Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Carabajal Torrez, José Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Molina, Fatima Romina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Fornaguera, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Martos, Gladys Irma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Bustos, Ana Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaFil: Gerez, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaSpringer2022-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184329Carrizo, Natalí Ivana; Carabajal Torrez, José Agustín; Molina, Fatima Romina Elizabeth; Fornaguera, Maria Jose; Martos, Gladys Irma; et al.; Selection and Performance of Antifungal Lactic Acid Bacteria in Corn Mini-Silos; Springer; Arabian Journal for Science and Engineering; 47; 1; 1-2022; 119-1302193-567X2191-4281CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s13369-021-05511-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:03Zoai:ri.conicet.gov.ar:11336/184329instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:04.313CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Selection and Performance of Antifungal Lactic Acid Bacteria in Corn Mini-Silos
title Selection and Performance of Antifungal Lactic Acid Bacteria in Corn Mini-Silos
spellingShingle Selection and Performance of Antifungal Lactic Acid Bacteria in Corn Mini-Silos
Carrizo, Natalí Ivana
ANTIFUNGAL ACTIVITY
FERMENTATION
LACTIC ACID BACTERIA
SILAGE
title_short Selection and Performance of Antifungal Lactic Acid Bacteria in Corn Mini-Silos
title_full Selection and Performance of Antifungal Lactic Acid Bacteria in Corn Mini-Silos
title_fullStr Selection and Performance of Antifungal Lactic Acid Bacteria in Corn Mini-Silos
title_full_unstemmed Selection and Performance of Antifungal Lactic Acid Bacteria in Corn Mini-Silos
title_sort Selection and Performance of Antifungal Lactic Acid Bacteria in Corn Mini-Silos
dc.creator.none.fl_str_mv Carrizo, Natalí Ivana
Carabajal Torrez, José Agustín
Molina, Fatima Romina Elizabeth
Fornaguera, Maria Jose
Martos, Gladys Irma
Bustos, Ana Yanina
Gerez, Carla Luciana
author Carrizo, Natalí Ivana
author_facet Carrizo, Natalí Ivana
Carabajal Torrez, José Agustín
Molina, Fatima Romina Elizabeth
Fornaguera, Maria Jose
Martos, Gladys Irma
Bustos, Ana Yanina
Gerez, Carla Luciana
author_role author
author2 Carabajal Torrez, José Agustín
Molina, Fatima Romina Elizabeth
Fornaguera, Maria Jose
Martos, Gladys Irma
Bustos, Ana Yanina
Gerez, Carla Luciana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIFUNGAL ACTIVITY
FERMENTATION
LACTIC ACID BACTERIA
SILAGE
topic ANTIFUNGAL ACTIVITY
FERMENTATION
LACTIC ACID BACTERIA
SILAGE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Silage is a method of forage conservation based on lactic fermentation. In order to improve the fermentative performance in corn silages, the aims of the present work were as follows: (I) to screen lactic acid bacteria with acidifying activity, (II) to study their antifungal activity against filamentous fungi and yeasts and (III) to evaluate their performance as antifungal in mini-silos. The acidifying activity was determined by measuring the pH and total titratable acidity. Those most acidifying bacteria were incubated in plates together with fungal markers strains to observe the presence or absence of inhibition halos, in addition to studying the nature of the antifungal metabolites by means of the treatment with proteases. The strain with the widest spectrum of action was selected to formulate an inoculum used in corn mini-silos. During storage, both silos determined humidity, acidification and microbiological composition, and then aerobic stability. The results showed that Lactoplantibacillus (L.) plantarum cultures had a higher production of lactic acid (7.7–17.5 g/L) than Pediococcus (8.8–10.9 g/L). L. plantarum CRL363 could inhibit the growth of filamentous fungi and some yeasts and consequently it was used in mini-silos. Compared to the control, the inoculated silo presented low pH values (4.02–4.85) and low fungi and yeast count until 20 days of storage, demonstrating the great potential of CRL363 strain for the formulation of inoculants for corn silage.
Fil: Carrizo, Natalí Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Carabajal Torrez, José Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Molina, Fatima Romina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Fornaguera, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Martos, Gladys Irma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Bustos, Ana Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: Gerez, Carla Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Silage is a method of forage conservation based on lactic fermentation. In order to improve the fermentative performance in corn silages, the aims of the present work were as follows: (I) to screen lactic acid bacteria with acidifying activity, (II) to study their antifungal activity against filamentous fungi and yeasts and (III) to evaluate their performance as antifungal in mini-silos. The acidifying activity was determined by measuring the pH and total titratable acidity. Those most acidifying bacteria were incubated in plates together with fungal markers strains to observe the presence or absence of inhibition halos, in addition to studying the nature of the antifungal metabolites by means of the treatment with proteases. The strain with the widest spectrum of action was selected to formulate an inoculum used in corn mini-silos. During storage, both silos determined humidity, acidification and microbiological composition, and then aerobic stability. The results showed that Lactoplantibacillus (L.) plantarum cultures had a higher production of lactic acid (7.7–17.5 g/L) than Pediococcus (8.8–10.9 g/L). L. plantarum CRL363 could inhibit the growth of filamentous fungi and some yeasts and consequently it was used in mini-silos. Compared to the control, the inoculated silo presented low pH values (4.02–4.85) and low fungi and yeast count until 20 days of storage, demonstrating the great potential of CRL363 strain for the formulation of inoculants for corn silage.
publishDate 2022
dc.date.none.fl_str_mv 2022-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184329
Carrizo, Natalí Ivana; Carabajal Torrez, José Agustín; Molina, Fatima Romina Elizabeth; Fornaguera, Maria Jose; Martos, Gladys Irma; et al.; Selection and Performance of Antifungal Lactic Acid Bacteria in Corn Mini-Silos; Springer; Arabian Journal for Science and Engineering; 47; 1; 1-2022; 119-130
2193-567X
2191-4281
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184329
identifier_str_mv Carrizo, Natalí Ivana; Carabajal Torrez, José Agustín; Molina, Fatima Romina Elizabeth; Fornaguera, Maria Jose; Martos, Gladys Irma; et al.; Selection and Performance of Antifungal Lactic Acid Bacteria in Corn Mini-Silos; Springer; Arabian Journal for Science and Engineering; 47; 1; 1-2022; 119-130
2193-567X
2191-4281
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s13369-021-05511-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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