Identification of resistant microorganisms and microbial activity in contaminated soils under three bioremediation approaches

Autores
Salinas, A.; Della Vedova, Maria Cecilia; Amieva, María Itatí; Lijteroff, Rubén Enrique; Delfini, Claudio Daniel; Villegas, Liliana Beatriz
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The success of a bioremediation process primarily depends on the intrinsic ability of the system to create and maintain conditions to promote the biodegradation of contaminants at a sufficiently high rate. Strategies to accelerate the biodegradation of hydrocarbons and other compounds in the soil include stimulating indigenous microorganisms (bio-stimulation) by optimizing factors such as the inoculation of a mixed microbial culture in the soil (bio-augmentation). The aim of this work was to identify effluent-resistant strains isolated from a landfarming and to evaluate three bioremediation approaches for a soil contaminated with glycols. For this, a series of laboratory-scale experiments were carried out with different experimental conditions: natural attenuation, biostimulation and bioaugmentation with previously selected native strains. The strains selected in previous studies were cultivated into LB-glucose (g L-1): NaCl 5.0; yeast extract 5.0; peptone 10.0; Glucose 10.0. The identification was realized by molecular techniques: DNA was obtained using a biology kit; PCR amplification with universal primers the DNA concentration in the PCR products was determined using Epoch (Biotek) and the integrity of the samples was evaluated through 1% Agarose gel electrophoresis. The PCR products were sent to CERELA (Tucuman-Argentina) for their purification and sequencing. The sequences were edited with Molecular Evolutionary Genetics Analysis (MEGA v7.0) and were analyzed with BLASTn using NCBI databases (www.ncbi.nlm.nih.gov). For the microbial activity test, 70 grams of soil were weighed in glass jars. Then, 4 ml of distilled water and 3 drops of Bromothymol Blue1 indicator were placed in the test tubes. The assembly of the devices was completed and then a “control” jar was made without the soil, to ensure that the change produced in the indicator was due to microbial respiration. The selected microorganisms were identified as coming: Penicillium, Bacillus and Acinetobacter. In the microbial activity test, different shades from yellow to green pH (6.7/7.4) could be seen: the one that received the mixed crop sowing presented lower pH values, indicating higher concentrations of CO2 coming from microbial respiration, followed by the biostimulation process and finally the natural attenuation process. Therefore, bioaugmentation and biostimulation increased microbial activity, indicating improved landfarming performance.
Fil: Salinas, A.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Della Vedova, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Amieva, María Itatí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Lijteroff, Rubén Enrique. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
XLII Reunión Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
Materia
Bioremediation
Contaminated soils
Resistant microorganisms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/275898
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/275898
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification of resistant microorganisms and microbial activity in contaminated soils under three bioremediation approachesSalinas, A.Della Vedova, Maria CeciliaAmieva, María ItatíLijteroff, Rubén EnriqueDelfini, Claudio DanielVillegas, Liliana BeatrizBioremediationContaminated soilsResistant microorganismshttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The success of a bioremediation process primarily depends on the intrinsic ability of the system to create and maintain conditions to promote the biodegradation of contaminants at a sufficiently high rate. Strategies to accelerate the biodegradation of hydrocarbons and other compounds in the soil include stimulating indigenous microorganisms (bio-stimulation) by optimizing factors such as the inoculation of a mixed microbial culture in the soil (bio-augmentation). The aim of this work was to identify effluent-resistant strains isolated from a landfarming and to evaluate three bioremediation approaches for a soil contaminated with glycols. For this, a series of laboratory-scale experiments were carried out with different experimental conditions: natural attenuation, biostimulation and bioaugmentation with previously selected native strains. The strains selected in previous studies were cultivated into LB-glucose (g L-1): NaCl 5.0; yeast extract 5.0; peptone 10.0; Glucose 10.0. The identification was realized by molecular techniques: DNA was obtained using a biology kit; PCR amplification with universal primers the DNA concentration in the PCR products was determined using Epoch (Biotek) and the integrity of the samples was evaluated through 1% Agarose gel electrophoresis. The PCR products were sent to CERELA (Tucuman-Argentina) for their purification and sequencing. The sequences were edited with Molecular Evolutionary Genetics Analysis (MEGA v7.0) and were analyzed with BLASTn using NCBI databases (www.ncbi.nlm.nih.gov). For the microbial activity test, 70 grams of soil were weighed in glass jars. Then, 4 ml of distilled water and 3 drops of Bromothymol Blue1 indicator were placed in the test tubes. The assembly of the devices was completed and then a “control” jar was made without the soil, to ensure that the change produced in the indicator was due to microbial respiration. The selected microorganisms were identified as coming: Penicillium, Bacillus and Acinetobacter. In the microbial activity test, different shades from yellow to green pH (6.7/7.4) could be seen: the one that received the mixed crop sowing presented lower pH values, indicating higher concentrations of CO2 coming from microbial respiration, followed by the biostimulation process and finally the natural attenuation process. Therefore, bioaugmentation and biostimulation increased microbial activity, indicating improved landfarming performance.Fil: Salinas, A.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Della Vedova, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Amieva, María Itatí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Lijteroff, Rubén Enrique. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaXLII Reunión Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/275898Identification of resistant microorganisms and microbial activity in contaminated soils under three bioremediation approaches; XLII Reunión Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2024; 1-2CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/reuniones-anuales-anteriores/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-03T08:37:08Zoai:ri.conicet.gov.ar:11336/275898instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-03 08:37:08.323CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of resistant microorganisms and microbial activity in contaminated soils under three bioremediation approaches
title Identification of resistant microorganisms and microbial activity in contaminated soils under three bioremediation approaches
spellingShingle Identification of resistant microorganisms and microbial activity in contaminated soils under three bioremediation approaches
Salinas, A.
Bioremediation
Contaminated soils
Resistant microorganisms
title_short Identification of resistant microorganisms and microbial activity in contaminated soils under three bioremediation approaches
title_full Identification of resistant microorganisms and microbial activity in contaminated soils under three bioremediation approaches
title_fullStr Identification of resistant microorganisms and microbial activity in contaminated soils under three bioremediation approaches
title_full_unstemmed Identification of resistant microorganisms and microbial activity in contaminated soils under three bioremediation approaches
title_sort Identification of resistant microorganisms and microbial activity in contaminated soils under three bioremediation approaches
dc.creator.none.fl_str_mv Salinas, A.
Della Vedova, Maria Cecilia
Amieva, María Itatí
Lijteroff, Rubén Enrique
Delfini, Claudio Daniel
Villegas, Liliana Beatriz
author Salinas, A.
author_facet Salinas, A.
Della Vedova, Maria Cecilia
Amieva, María Itatí
Lijteroff, Rubén Enrique
Delfini, Claudio Daniel
Villegas, Liliana Beatriz
author_role author
author2 Della Vedova, Maria Cecilia
Amieva, María Itatí
Lijteroff, Rubén Enrique
Delfini, Claudio Daniel
Villegas, Liliana Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bioremediation
Contaminated soils
Resistant microorganisms
topic Bioremediation
Contaminated soils
Resistant microorganisms
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The success of a bioremediation process primarily depends on the intrinsic ability of the system to create and maintain conditions to promote the biodegradation of contaminants at a sufficiently high rate. Strategies to accelerate the biodegradation of hydrocarbons and other compounds in the soil include stimulating indigenous microorganisms (bio-stimulation) by optimizing factors such as the inoculation of a mixed microbial culture in the soil (bio-augmentation). The aim of this work was to identify effluent-resistant strains isolated from a landfarming and to evaluate three bioremediation approaches for a soil contaminated with glycols. For this, a series of laboratory-scale experiments were carried out with different experimental conditions: natural attenuation, biostimulation and bioaugmentation with previously selected native strains. The strains selected in previous studies were cultivated into LB-glucose (g L-1): NaCl 5.0; yeast extract 5.0; peptone 10.0; Glucose 10.0. The identification was realized by molecular techniques: DNA was obtained using a biology kit; PCR amplification with universal primers the DNA concentration in the PCR products was determined using Epoch (Biotek) and the integrity of the samples was evaluated through 1% Agarose gel electrophoresis. The PCR products were sent to CERELA (Tucuman-Argentina) for their purification and sequencing. The sequences were edited with Molecular Evolutionary Genetics Analysis (MEGA v7.0) and were analyzed with BLASTn using NCBI databases (www.ncbi.nlm.nih.gov). For the microbial activity test, 70 grams of soil were weighed in glass jars. Then, 4 ml of distilled water and 3 drops of Bromothymol Blue1 indicator were placed in the test tubes. The assembly of the devices was completed and then a “control” jar was made without the soil, to ensure that the change produced in the indicator was due to microbial respiration. The selected microorganisms were identified as coming: Penicillium, Bacillus and Acinetobacter. In the microbial activity test, different shades from yellow to green pH (6.7/7.4) could be seen: the one that received the mixed crop sowing presented lower pH values, indicating higher concentrations of CO2 coming from microbial respiration, followed by the biostimulation process and finally the natural attenuation process. Therefore, bioaugmentation and biostimulation increased microbial activity, indicating improved landfarming performance.
Fil: Salinas, A.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Della Vedova, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Amieva, María Itatí. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Lijteroff, Rubén Enrique. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Delfini, Claudio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
XLII Reunión Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
description The success of a bioremediation process primarily depends on the intrinsic ability of the system to create and maintain conditions to promote the biodegradation of contaminants at a sufficiently high rate. Strategies to accelerate the biodegradation of hydrocarbons and other compounds in the soil include stimulating indigenous microorganisms (bio-stimulation) by optimizing factors such as the inoculation of a mixed microbial culture in the soil (bio-augmentation). The aim of this work was to identify effluent-resistant strains isolated from a landfarming and to evaluate three bioremediation approaches for a soil contaminated with glycols. For this, a series of laboratory-scale experiments were carried out with different experimental conditions: natural attenuation, biostimulation and bioaugmentation with previously selected native strains. The strains selected in previous studies were cultivated into LB-glucose (g L-1): NaCl 5.0; yeast extract 5.0; peptone 10.0; Glucose 10.0. The identification was realized by molecular techniques: DNA was obtained using a biology kit; PCR amplification with universal primers the DNA concentration in the PCR products was determined using Epoch (Biotek) and the integrity of the samples was evaluated through 1% Agarose gel electrophoresis. The PCR products were sent to CERELA (Tucuman-Argentina) for their purification and sequencing. The sequences were edited with Molecular Evolutionary Genetics Analysis (MEGA v7.0) and were analyzed with BLASTn using NCBI databases (www.ncbi.nlm.nih.gov). For the microbial activity test, 70 grams of soil were weighed in glass jars. Then, 4 ml of distilled water and 3 drops of Bromothymol Blue1 indicator were placed in the test tubes. The assembly of the devices was completed and then a “control” jar was made without the soil, to ensure that the change produced in the indicator was due to microbial respiration. The selected microorganisms were identified as coming: Penicillium, Bacillus and Acinetobacter. In the microbial activity test, different shades from yellow to green pH (6.7/7.4) could be seen: the one that received the mixed crop sowing presented lower pH values, indicating higher concentrations of CO2 coming from microbial respiration, followed by the biostimulation process and finally the natural attenuation process. Therefore, bioaugmentation and biostimulation increased microbial activity, indicating improved landfarming performance.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/275898
Identification of resistant microorganisms and microbial activity in contaminated soils under three bioremediation approaches; XLII Reunión Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2024; 1-2
CONICET Digital
CONICET
url http://hdl.handle.net/11336/275898
identifier_str_mv Identification of resistant microorganisms and microbial activity in contaminated soils under three bioremediation approaches; XLII Reunión Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2024; 1-2
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/reuniones-anuales-anteriores/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad de Biología de Cuyo
publisher.none.fl_str_mv Sociedad de Biología de Cuyo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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