Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorillonite

Autores
Magnoli, Alejandra Paola; Alonso, Veronica Andrea; Cavaglieri, Lilia Reneé; Dalcero, Ana Maria; Chiacchiera, Stella Maris
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The main objective of this study was to evaluate the interference of environment components on the in vitro evaluation of aflatoxin B1 adsorption capacity of sodium bentonite under simulated gastrointestinal conditions of monogastric and ruminant animals. Sodium bentonite showed a high aflatoxin B1 affinity with all of the assays. Langmuir or sigmoid isotherms were found in different assays. Both the affinities and the surface excesses at monolayer saturation were affected by the buffer components. The specific influence of ions in each buffer solution was investigated. A significant decrease in the surface excess at monolayer saturation was observed under ionic strength control. A change in the isotherm shape from sigmoidal to Langmuir was observed with the increase in the sodium chloride concentration. This was attributed to the decrease in the importance of lateral interaction between adsorbed toxin molecules compared with surface-molecules interactions under a high salt coverage. The presence of rumen fluid components in the adsorption environment decreased the aflatoxin B1 maximum adsorption capacity of sodium bentonite. Despite the high affinity of this adsorbent to capture aflatoxin B1, different substances present in the environment could affect the adsorption capacity, at least at low toxin concentrations that mimic chronic exposure. The environment of the gastrointestinal tract, in either monogastric or ruminant animals, affect in vivo aflatoxin B1 adsorption by sodium bentonite and should be taken into account when an in vitro performance evaluation is done.
Fil: Magnoli, Alejandra Paola. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso, Veronica Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chiacchiera, Stella Maris. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Aflatoxina B1
Sodium Bentonite
Adsorption
Hplc
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22952
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/22952
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorilloniteMagnoli, Alejandra PaolaAlonso, Veronica AndreaCavaglieri, Lilia ReneéDalcero, Ana MariaChiacchiera, Stella MarisAflatoxina B1Sodium BentoniteAdsorptionHplchttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The main objective of this study was to evaluate the interference of environment components on the in vitro evaluation of aflatoxin B1 adsorption capacity of sodium bentonite under simulated gastrointestinal conditions of monogastric and ruminant animals. Sodium bentonite showed a high aflatoxin B1 affinity with all of the assays. Langmuir or sigmoid isotherms were found in different assays. Both the affinities and the surface excesses at monolayer saturation were affected by the buffer components. The specific influence of ions in each buffer solution was investigated. A significant decrease in the surface excess at monolayer saturation was observed under ionic strength control. A change in the isotherm shape from sigmoidal to Langmuir was observed with the increase in the sodium chloride concentration. This was attributed to the decrease in the importance of lateral interaction between adsorbed toxin molecules compared with surface-molecules interactions under a high salt coverage. The presence of rumen fluid components in the adsorption environment decreased the aflatoxin B1 maximum adsorption capacity of sodium bentonite. Despite the high affinity of this adsorbent to capture aflatoxin B1, different substances present in the environment could affect the adsorption capacity, at least at low toxin concentrations that mimic chronic exposure. The environment of the gastrointestinal tract, in either monogastric or ruminant animals, affect in vivo aflatoxin B1 adsorption by sodium bentonite and should be taken into account when an in vitro performance evaluation is done.Fil: Magnoli, Alejandra Paola. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alonso, Veronica Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chiacchiera, Stella Maris. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaTaylor & Francis2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22952Magnoli, Alejandra Paola; Alonso, Veronica Andrea; Cavaglieri, Lilia Reneé; Dalcero, Ana Maria; Chiacchiera, Stella Maris; Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorillonite; Taylor & Francis; Food Additives And Contaminants. Part B - Surveillance Communications; 30; 4; 4-2013; 743-7491939-3210CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/19440049.2013.784398info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/19440049.2013.784398info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:15:45Zoai:ri.conicet.gov.ar:11336/22952instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:15:46.061CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorillonite
title Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorillonite
spellingShingle Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorillonite
Magnoli, Alejandra Paola
Aflatoxina B1
Sodium Bentonite
Adsorption
Hplc
title_short Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorillonite
title_full Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorillonite
title_fullStr Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorillonite
title_full_unstemmed Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorillonite
title_sort Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorillonite
dc.creator.none.fl_str_mv Magnoli, Alejandra Paola
Alonso, Veronica Andrea
Cavaglieri, Lilia Reneé
Dalcero, Ana Maria
Chiacchiera, Stella Maris
author Magnoli, Alejandra Paola
author_facet Magnoli, Alejandra Paola
Alonso, Veronica Andrea
Cavaglieri, Lilia Reneé
Dalcero, Ana Maria
Chiacchiera, Stella Maris
author_role author
author2 Alonso, Veronica Andrea
Cavaglieri, Lilia Reneé
Dalcero, Ana Maria
Chiacchiera, Stella Maris
author2_role author
author
author
author
dc.subject.none.fl_str_mv Aflatoxina B1
Sodium Bentonite
Adsorption
Hplc
topic Aflatoxina B1
Sodium Bentonite
Adsorption
Hplc
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The main objective of this study was to evaluate the interference of environment components on the in vitro evaluation of aflatoxin B1 adsorption capacity of sodium bentonite under simulated gastrointestinal conditions of monogastric and ruminant animals. Sodium bentonite showed a high aflatoxin B1 affinity with all of the assays. Langmuir or sigmoid isotherms were found in different assays. Both the affinities and the surface excesses at monolayer saturation were affected by the buffer components. The specific influence of ions in each buffer solution was investigated. A significant decrease in the surface excess at monolayer saturation was observed under ionic strength control. A change in the isotherm shape from sigmoidal to Langmuir was observed with the increase in the sodium chloride concentration. This was attributed to the decrease in the importance of lateral interaction between adsorbed toxin molecules compared with surface-molecules interactions under a high salt coverage. The presence of rumen fluid components in the adsorption environment decreased the aflatoxin B1 maximum adsorption capacity of sodium bentonite. Despite the high affinity of this adsorbent to capture aflatoxin B1, different substances present in the environment could affect the adsorption capacity, at least at low toxin concentrations that mimic chronic exposure. The environment of the gastrointestinal tract, in either monogastric or ruminant animals, affect in vivo aflatoxin B1 adsorption by sodium bentonite and should be taken into account when an in vitro performance evaluation is done.
Fil: Magnoli, Alejandra Paola. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso, Veronica Andrea. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chiacchiera, Stella Maris. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The main objective of this study was to evaluate the interference of environment components on the in vitro evaluation of aflatoxin B1 adsorption capacity of sodium bentonite under simulated gastrointestinal conditions of monogastric and ruminant animals. Sodium bentonite showed a high aflatoxin B1 affinity with all of the assays. Langmuir or sigmoid isotherms were found in different assays. Both the affinities and the surface excesses at monolayer saturation were affected by the buffer components. The specific influence of ions in each buffer solution was investigated. A significant decrease in the surface excess at monolayer saturation was observed under ionic strength control. A change in the isotherm shape from sigmoidal to Langmuir was observed with the increase in the sodium chloride concentration. This was attributed to the decrease in the importance of lateral interaction between adsorbed toxin molecules compared with surface-molecules interactions under a high salt coverage. The presence of rumen fluid components in the adsorption environment decreased the aflatoxin B1 maximum adsorption capacity of sodium bentonite. Despite the high affinity of this adsorbent to capture aflatoxin B1, different substances present in the environment could affect the adsorption capacity, at least at low toxin concentrations that mimic chronic exposure. The environment of the gastrointestinal tract, in either monogastric or ruminant animals, affect in vivo aflatoxin B1 adsorption by sodium bentonite and should be taken into account when an in vitro performance evaluation is done.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22952
Magnoli, Alejandra Paola; Alonso, Veronica Andrea; Cavaglieri, Lilia Reneé; Dalcero, Ana Maria; Chiacchiera, Stella Maris; Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorillonite; Taylor & Francis; Food Additives And Contaminants. Part B - Surveillance Communications; 30; 4; 4-2013; 743-749
1939-3210
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22952
identifier_str_mv Magnoli, Alejandra Paola; Alonso, Veronica Andrea; Cavaglieri, Lilia Reneé; Dalcero, Ana Maria; Chiacchiera, Stella Maris; Effect of monogastric and ruminant gastrointestinal conditions on in vitro aflatoxin B1 adsorption ability by a montmorillonite; Taylor & Francis; Food Additives And Contaminants. Part B - Surveillance Communications; 30; 4; 4-2013; 743-749
1939-3210
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/19440049.2013.784398
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.1080/19440049.2013.784398
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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